Understanding the spectral energy distributions of the galactic star forming regions IRAS 18314-0720, 18355-0532 & 18316-0602

Embedded Young Stellar Objects (YSO) in dense interstellar clouds is treated self-consistently to understand their spectral energy distributions (SED). Radiative transfer calculations in spherical geometry involving the dust as well as the gas component, have been carried out to explain observations covering a wide spectral range encompassing near-infrared to radio continuum wavelengths. Various geometric and physical details of the YSOs are determined from this modelling scheme. In order to assess the effectiveness of this self-consistent scheme, three young Galactic star forming regions associated with IRAS 18314-0720, 18355-0532 and 18316-0602 have been modelled as test cases. They cover a large range of luminosity (≈ 40). The modelling of their SEDs has led to information about various details of these sources, e.g. embedded energy source, cloud structure & size, density distribution, composition & abundance of dust grains etc. In all three cases, the best fit model corresponds to the uniform density distribution

Decade-long timing of four GMRT discovered millisecond pulsars

The discovery and timing follow-up of millisecond pulsars (MSPs) are necessary not just for their usefulness in Pulsar Timing Arrays (PTAs) but also for investigating their own intriguing properties. In this work, we provide the findings of the decade-long timing of the four MSPs discovered by the Giant Metre-wave Radio Telescope (GMRT), including their timing precision, model parameters, and newly detected proper motions. We compare the timing results for these MSPs before and after the GMRT upgrade in 2017, characterise the improvement in timing precision due to the bandwidth upgrade. We discuss the suitability of these four GMRT MSPs as well as the usefulness of their decade-long timing data for the PTA {experiments. It may aid} in the global effort to improve the signal-to-noise (S/N) of recently detected signature of gravitational waves in cross-correlation statistics of residuals of MSPs.Comment: Accepted in Astrophysical Journal (APJ) on October 31, 202

The fine structure line deficit in S 140

We try to understand the gas heating and cooling in the S 140 star forming region by spatially and spectrally resolving the distribution of the main cooling lines with GREAT/SOFIA. We mapped the fine structure lines of [OI] (63 {\mu}m) and [CII] (158 {\mu}m) and the rotational transitions of CO 13-12 and 16-15 with GREAT/SOFIA and analyzed the spatial and velocity structure to assign the emission to individual heating sources. We measure the optical depth of the [CII] line and perform radiative transfer computations for all observed transitions. By comparing the line intensities with the far-infrared continuum we can assess the total cooling budget and measure the gas heating efficiency. The main emission of fine structure lines in S 140 stems from a 8.3'' region close to the infrared source IRS 2 that is not prominent at any other wavelength. It can be explained by a photon-dominated region (PDR) structure around the embedded cluster if we assume that the [OI] line intensity is reduced by a factor seven due to self-absorption. The external cloud interface forms a second PDR at an inclination of 80-85 degrees illuminated by an UV field of 60 times the standard interstellar radiation field. The main radiation source in the cloud, IRS 1, is not prominent at all in the fine structure lines. We measure line-to-continuum cooling ratios below 10^(-4), i.e. values lower than in any other Galactic source, rather matching the far-IR line deficit seen in ULIRGs. In particular the low intensity of the [CII] line can only be modeled by an extreme excitation gradient in the gas around IRS 1. We found no explanation why IRS 1 shows no associated fine-structure line peak, while IRS 2 does. The inner part of S 140 mimics the far-IR line deficit in ULIRGs thereby providing a template that may lead to a future model.Comment: Accepted for publication in Astronomy & Astrophysic

Method development and validation for acrylamide in potato cutlet by UHPLC-MS/MS

Surge in consumption of healthy and safe foods has challenged researchers to develop sensitive, precise, robust detection and quantification of food contaminants like acrylamide even if they are present in trace. Keeping this in view, a robust and sensitive analytical method was developed and validated for acrylamide quantification in a potato-based food product (potato cutlet), using LC-MSMS with positive electrospray ionization (+ESI). The method consists of adding acrylamide-d3 (deuterium labelled acrylamide) as an internal standard, extraction by modified QuEChERS method with d-SPE clean-up. Extracts were run on a reverse phase C-18 column for analysis by liquid chromatography. The limit of detection (LOD) and limit of quantification (LOQ) were determined as 0.7 and 2.0 μg kg− 1 , respectively, demonstrating the sensitivity of the method for trace detection. The developed method showed excellent results in terms of recoveries (91.0–109.16%), repeatability (RSD 1.8–10.60%), reproducibility (RSD 2.3–11.24%) and robustness (RSD 1.74–4.54%). The repeatability, reproducibility and robustness respectively, show the consistency, accuracy and stability of the developed method. The deviations in ion ratio and retention time were 17.25% and − 0.01 min, respectively. Compared to previous findings, the current study has achieved lower LOD and LOQ levels that reveals the higher sensitivity of this method for acrylamide quantification in potato cutletMinistry of Food Processing Industries, Government of India | Ref. Q-29/11/2018-R&DUniversidade de Vigo/CISU

Understanding the Spectral Energy Distributions of the Galactic Star Forming Regions IRAS 18314-0720, 18355-0532 & 18316-0602

Embedded Young Stellar Objects (YSO) in dense interstellar clouds is treated self-consistently to understand their spectral energy distributions (SED). Radiative transfer calculations in spherical geometry involving the dust as well as the gas component, have been carried out to explain observations covering a wide spectral range encompassing near-infrared to radio continuum wavelengths. Various geometric and physical details of the YSOs are determined from this modelling scheme. In order to assess the effectiveness of this self-consistent scheme, three young Galactic star forming regions associated with IRAS 18314-0720, 18355-0532 and 18316-0602 have been modelled as test cases. They cover a large range of luminosity ($\approx$ 40). The modelling of their SEDs has led to information about various details of these sources, e.g. embedded energy source, cloud structure & size, density distribution, composition & abundance of dust grains etc. In all three cases, the best fit model corresponds to the uniform density distribution.Comment: AAMS style manuscript with 3 tables (in a separate file) and 4 figures. To appear in Journal of Astronophysics & Astronom

Establishment of reference CD4+ T cell values for adult Indian population

<p>Abstract</p> <p>Background</p> <p>CD4+ T lymphocyte counts are the most important indicator of disease progression and success of antiretroviral treatment in HIV infection in resource limited settings. The nationwide reference range of CD4+ T lymphocytes was not available in India. This study was conducted to determine reference values of absolute CD4+ T cell counts and percentages for adult Indian population.</p> <p>Methods</p> <p>A multicentric study was conducted involving eight sites across the country. A total of 1206 (approximately 150 per/centre) healthy participants were enrolled in the study. The ratio of male (N = 645) to female (N = 561) of 1.14:1. The healthy status of the participants was assessed by a pre-decided questionnaire. At all centers the CD4+ T cell count, percentages and absolute CD3+ T cell count and percentages were estimated using a single platform strategy and lyse no wash technique. The data was analyzed using the Statistical Package for the Social Scientist (SPSS), version 15) and Prism software version 5.</p> <p>Results</p> <p>The absolute CD4+ T cell counts and percentages in female participants were significantly higher than the values obtained in male participants indicating the true difference in the CD4+ T cell subsets. The reference range for absolute CD4 count for Indian male population was 381-1565 cells/μL and for female population was 447-1846 cells/μL. The reference range for CD4% was 25-49% for male and 27-54% for female population. The reference values for CD3 counts were 776-2785 cells/μL for Indian male population and 826-2997 cells/μL for female population.</p> <p>Conclusion</p> <p>The study used stringent procedures for controlling the technical variation in the CD4 counts across the sites and thus could establish the robust national reference ranges for CD4 counts and percentages. These ranges will be helpful in staging the disease progression and monitoring antiretroviral therapy in HIV infection in India.</p

Evaluation of Two Internalizing Carcinoembryonic Antigen Reporter Genes for Molecular Imaging

PurposeThe objective of this article is to develop internalizing positron emission tomography (PET) reporter genes for tracking genetically modified T cells in vivo.ProceduresThe transmembrane and cytoplasmic domains of the human transferrin receptor (TfR) and CD5 were each fused to the carcinoembryonic (CEA) minigene N-A3 and expressed in Jurkat T cells. Internalization was evaluated by confocal microscopy or by intracellular uptake of ¹²⁵I-labeled anti-CEA scFv-Fc. Reporter gene-transfected Jurkat xenografts in mice were analyzed by immunohistochemistry (IHC) and imaged by PET using ¹²⁴I- or ⁶⁴Cu-scFv-Fc as tracers.ResultsSurface expression of TR(1-99)-NA3 was lower than that of NA3-CD5. Both reporter genes were internalized following binding of the anti-CEA antibody fragment. IHC of tumors showed strong staining of NA3-CD5, whereas TR(1-99)-NA3 stained weakly. Specific targeting of TR(1-99)-NA3 or NA3-CD5 was shown by PET in xenografted mice.ConclusionsThe in vivo imaging studies suggest a potential application of the internalizing form of CEA (N-A3) as a PET reporter gene

A detailed Hapmap of the Sitosterolemia locus spanning 69 kb; differences between Caucasians and African-Americans

BACKGROUND: Sitosterolemia is an autosomal recessive disorder that maps to the sitosterolemia locus, STSL, on human chromosome 2p21. Two genes, ABCG5 and ABCG8, comprise the STSL and mutations in either cause sitosterolemia. ABCG5 and ABCG8 are thought to have evolved by gene duplication event and are arranged in a head-to-head configuration. We report here a detailed characterization of the STSL in Caucasian and African-American cohorts. METHODS: Caucasian and African-American DNA samples were genotypes for polymorphisms at the STSL locus and haplotype structures determined for this locus RESULTS: In the Caucasian population, 13 variant single nucleotide polymorphisms (SNPs) were identified and resulting in 24 different haplotypes, compared to 11 SNPs in African-Americans resulting in 40 haplotypes. Three polymorphisms in ABCG8 were unique to the Caucasian population (E238L, INT10-50 and G575R), whereas one variant (A259V) was unique to the African-American population. Allele frequencies of SNPs varied also between these populations. CONCLUSION: We confirmed that despite their close proximity to each other, significantly more variations are present in ABCG8 compared to ABCG5. Pairwise D' values showed wide ranges of variation, indicating some of the SNPs were in strong linkage disequilibrium (LD) and some were not. LD was more prevalent in Caucasians than in African-Americans, as would be expected. These data will be useful in analyzing the proposed role of STSL in processes ranging from responsiveness to cholesterol-lowering drugs to selective sterol absorption

Natural Abundant Solid State NMR Studies in Designed Tripeptides for Differentiation of Multiple Conformers

Solid state NMR (SSNMR) experiments on heteronuclei in natural abundance are described for three synthetically designed tripeptides Piv-(L)Pro_(L)Pro-(L)Phe-OMe (1), Piv-(D)Pro_(L)Pro_(L)Phe-OMe (2), and Piv-(D)Pro_(L)Pro_(L)Phe-NHMe (3). These peptides exist in different conformation as shown by solution state NMR and single crystal X-ray analysis (Chatterjee et al., Chem Eur J 2008, 14, 6192). In this study, SSNMR has been used to probe the conformations of these peptides in their powder form. The C-13 spectrum of peptide (1) showed doubling of resonances corresponding to cis/cis form, unlike in solution where the similar doubling is attributed to cis/trans form. This has been confirmed by the chemical shift differences of C-beta and C-gamma carbon of Proline in peptide (1) both in solution and SSNMR. Peptide (2) and (3) provided single set of resonances which represented all transform across the di-Proline segment. The results are In agreement with the X-ray analysis. Solid state N-15 resonances, especially from Proline residues provided additional information, which is normally not observable in solution state NMR. H-1 chemical shifts are also obtained from a two-dimensional heteronuclear correlation experiment between H-1-C-13. The results confirm the utility of NMR as a useful tool for identifying different conformers in peptides in the solid state. (C) 2009 Wiley Periodicals, Inc. Biopolymers 91: 851-860, 2009