37 research outputs found
Conformational dynamics of a single protein monitored for 24 hours at video rate
We use plasmon rulers to follow the conformational dynamics of a single
protein for up to 24 h at a video rate. The plasmon ruler consists of two gold
nanospheres connected by a single protein linker. In our experiment, we follow
the dynamics of the molecular chaperone heat shock protein 90, which is known
to show open and closed conformations. Our measurements confirm the previously
known conformational dynamics with transition times in the second to minute
time scale and reveals new dynamics on the time scale of minutes to hours.
Plasmon rulers thus extend the observation bandwidth 3/4 orders of magnitude
with respect to single-molecule fluorescence resonance energy transfer and
enable the study of molecular dynamics with unprecedented precision
METHOD FOR DETECTING AND/OR QUANTIFYING THE BINDING AFFINITIES OF A TARGET MOLECULE TO A PLURALITY OF DIFFERENT BINDING PARTNERS BY PLASMON RESONANCE OF NANOPARTICLES AND A POSITION-ENCODED SENSOR THEREFORE
Depto. de QuĂmica FĂsicaFac. de Ciencias QuĂmicaspu
Alivisatos. Controlled synthesis of hyper-branched inorganic nanocrystals with rich three-dimentional structures. Nano Lett
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