Testicular morphology of adult wistar rats treated with Rudgea viburnoides (Cham.) Benth. leaf infusion

The present study was carried out to investigate the effects of Rudgea viburnoides infusion on the body biometry and testicular morphometry and stereology of adult Wistar rats. Two groups received the infusion daily at the concentration of 3 or 6 mg/mL for 40 days. The control group received only water. Neither the biometrical parameters nor the tubular diameter and the height of the seminiferous epithelium showed any significant alterations in the treated animals. Leydig cells stereology did not show any significant alterations in the treated animals. These results indicated that R. viburnoides did not cause alterations in body biometry and testicular morphometry.10110

Hystometric evaluation of nickel chronic exposure effects on large instestine of adult Wistar male rats

The ingestion of considerable amounts of water or food contaminated with nickel can be very toxic. The present work was conducted aiming to evaluate the effects of nickel exposures on ascending colon of adult Wistar male rats at hystometric level. We used 12 animals that were divided in a control (ingested uncontaminated water) and a nickel-contaminated (i.e., 25 mg de nickel/L of water) groups. Nickel chloride was offered in declorinated water and the experiment had a 56 days exposure period. A portion of the ascending colon was removed of the animals and subjected to hystological labelling processes using blue toluidin (for general hystometric description), Alcian Blue (AB, for acid mucins) and periodic acid-Schiff (PAS) technique (for neutral mucins). The potential differences between groups were desgined by applying the Whitney test and t test (p < 0.05). The crypts were smaller for the nickel-contaminated group, even though these organism exhibited broader and higher crypts. Nickel-contaminated animals exhibited a smaller amount of calyceform cells with AB and PAS positive reactions as well as a less mucus quantities when compared with nickel-uncontaminated animals. Such reductions on the amount of calyceform cells with AB and PAS positive reactions may be related wiht the shallower crypts, which possibly reduced the synthesis and secretion of mucins, compromissing the functional aspects (e.g., lubrification and intestinal mucosa protection) of the nickel-contaminated large intestines. Interestingly, the wider and higher crypts and higher epithelium collumn on the nickel-contaminated animals may represent a relevant trade-off for the intestinal mucosa protection

Microscopic morphology and testis morphometry of captivity- bred Adult bullfrogs ( Lithobates catesbeianus Shaw, 1802)

The aim of this work was to study the testicular morphometry of captivity-bred adult bullfrogs. Fifteen young adult male were studied, in the rainy season and a lengthy photoperiod. The GSI was established at 0.15%. The nuclear diameter of germinative and Leydig cells, the nucleolus diameter of Sertoli cells and the area of cysts and tubules were determined and the mean number of ISPC, IISPC and SPT per cyst and the mean number of cysts per tubule was estimated. The nucleoplasmatic proportion of the nucleus of the Leydig cell was 76.22%, indicating less cytoplasmic activity. Eight generations of spermatogonia were found. The spermatogenesis efficiency in meiosis and in mitosis was 63 and 49%, respectively. The spermatogenesis of bullfrog fited in the pattern of other captivity Anurans, with differences as the morphology of Sertoli and Leydig cells nuclei.A morfometria é uma importante ferramenta para a biologia estrutural, permitindo estudos estereológicos e análises quantitativas. Existem muitos pontos a serem esclarecidos sobre a morfometria testicular desta espécie, que objetivamos desvendar neste trabalho. Quinze machos adultos foram estudados, em período chuvoso e de fotoperíodo longo (dezembro, 2000). O IGS encontrado foi de 0.15%. O diâmetro nuclear das células germinativas e da célula de Leydig, o diâmetro nucleolar das células de Sertoli e a área dos cistos e túbulos foram determinados. O número médio de ISPC, IISPC e SPT por cisto e o número médio de cisto por túbulo foi estimado. A proporção nucleoplasmática do núcleo da célula de Leydig foi de 76.22%, indicando pouca atividade citoplasmática. Oito gerações de espermatogônia foram estimadas. A eficiência da espermatogênese na meiose e mitose foi de 63% e49%, respectivamente. A espermatogênese de rãtouro segue os padrões dos demais Anuros de cativeiro, apresentando diferenças nos núcleos das células de Sertoli e Leydig

Aspectos reprodutivos de Dendropsophus minutus (PETERS, 1872) no município de Viçosa, Minas Gerais

A biologia reprodutiva de machos de Dendropsophus minutus foi estudada em Viçosa, Minas Gerais, no período de fevereiro de 2005 a janeiro de 2006. Procurou-se estabelecer o ciclo reprodutivo da espécie através da análise conjunta da histologia dos testículos e dos aspectos reprodutivos observados em campo, como distribuição temporal e espacial da espécie, abundância de machos em vocalização, casais em amplexo, desovas, e presença de formas larvais, juvenis e adultas. Vocalizações foram observadas ao longo de todo o período experimental, enquanto formas juvenis foram avistadas de setembro a abril. Nenhum casal em amplexo, nem desovas e girinos foram visualizados. Áreas recobertas por vegetação aquática e áreas marginais com gramíneas constituíram os principais sítios reprodutivos da espécie. Machos de D. minutus apresentaram variações anuais significativas no comprimento corporal, peso corporal, massa gonadal e no índice gonadossomático. Espermátides e espermatozóides foram observados nos túbulos seminíferos em todos os meses do ano, mostrando sincronia na atividade testicular. A partir da histologia dos testículos e dos aspectos reprodutivos observados em campo, podemos concluir que D. minutus possui ciclo reprodutivo prolongado, com maior potencial reprodutivo entre os meses de junho e dezembro.The reproductive biology of male Dendropsophus minutus was studied in Viçosa, Minas Gerais, from 2005 February to 2006 January. This study aimed to establish the reproductive cycle of the specie through a combined analysis of testicular histology and field observations, such as temporal and spatial distribution of specie, abundance of calling males, occurrence of amplectant pairs, spawning, and presence of larval, juveniles, and adult forms. Calls were detected during all the experimental period while juvenile forms were visualized from September to April. During this study neither amplectant pairs nor spawning and tadpole were visualized. Areas covered by aquatic vegetation as well as the border areas around the dam were the most used location for the specie ́s reproduction. Males of D. minutus showed annual variations in body length, body weight, gonadal mass and gonadosomatic index. Spermatids and spermatozoa were observed monthly in the seminiferous tubules, showing the continue pattern of reproduction. Based in testicular histology combined to reproductive field aspects we conclude that D.minutus have a prolonged reproductive cycle and peak of reproduction between June and December

Níveis de energia digestível na dieta de piauçu (Leporinus macrocephalus) no desenvolvimento testicular em estágio pós-larval

Objetivando-se determinar o efeito do nível de energia digestível da dieta no desenvolvimento testicular de piauçu (Leporinus macrocephalus) foram utilizados 600 peixes com peso e comprimento médio inicial de 0,33 ± 0,11 g e 2,94 ± 0,39 cm, respectivamente. Foram realizados quatro tratamentos (2.600, 2.700, 2.800 e 2.900 kcal de ED/kg de ração). Verificou-se que o nível de energia não influenciou o peso corporal médio final e comprimento total. Foi verificado efeito quadrático (P<0,02) no peso da gônada aos 60 dias. Foi verificado efeito quadrático (P<0,02) no índice gonadossomático aos 60 dias. Os peixes do tratamentos com 2.800 e 2.900 kcal de ED/kg apresentaram nítida formação de cistos de espermatogônias primárias e secundárias. Os animais dos grupos com 2.600 e 2.700 kcal de ED/kg apresentaram formação de cistos de espermatogônias primária, apenas

Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae)

Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT), alanine transaminase (ALT), aspartate transaminase (AST), chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG) occurred in the group that received the higher dose