MICROBIOTA INTESTINAL E ASSOCIAÇÕES COM DESORDENS CLÍNICAS EM FUNÇÃO DA FAIXA ETÁRIA DE IDOSOS: UM ESTUDO ANALÍTICO TRANSVERSAL

O envelhecimento da população e as projeções para o aumento crescente de idosos é uma realidade mundial, que desperta interesse da comunidade científica no intuito de esclarecer os aspectos relacionados ao envelhecimento saudável. As características da microbiota intestinal podem estar relacionadas com indicadores de saúde/doença e a sua relação com o processo de envelhecimento é parcialmente compreendida. O objetivo deste estudo foi quantificar dois grupos de bactérias intestinais em indivíduos idosos institucionalizados, em função da faixa etária, e verificar possíveis associações a variáveis clínicas. Trata-se de uma pesquisa quantitativa, analítica e transversal, realizada com 62 idosos residentes em uma instituição de longa permanência para idosos no estado de Minas Gerais, Brasil. Foram realizadas análises parasitológica e microbiológica em amostras de fezes e aplicação de um questionário individual. Os resultados demonstraram um aumento de enterobactérias totais com o avançar da idade em mulheres de 70-79 anos com relação aos homens da mesma faixa etária. Verificou-se, ainda, que os idosos com idade superior a 80 anos e dependentes nas atividades de vida diária apresentam um aumento de lactobacilos presuntivos. Não se observou relação significativa entre gêneros e contagens de lactobacilos presuntivos nem houve associação entre as contagens e constipação intestinal, incontinência urinária e hipertensão

Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country

Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is difficult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identified by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identification at the species level. Microbial identification revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied

Agreement between RAPD, API20C AUX, CHROMagar Candida and microculture on oral Candida identification

Abstract Aim: To measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. After this, agreement among methods using Kappa test was performed. Results: Agreement rates between RAPD and CHROMagar Candida, showed significant accuracy for C. albicans, C. tropicalis, C. dubliniensis and C. krusei (Kappa: 0.760, 0.640, 0.416 and 0.360, respectively, p<0.05). Comparing RAPD results with microculture, the highest agreement was for C. albicans (Kappa: 0.851 -p<0.05) but no significant agreement for C. lusitaniae, C. krusei and C. guilliermondii was obtained (p>0.05). The agreement was significant for all identified species when RAPD (OPE-18) and API 20C AUX (p<0.05) were used. Critical levels of agreement between RAPD and microculture were observed when C. lusitaniae, C. krusei and C. guilliermondii were identified. Conclusions: API 20C AUX presented the best agreement with molecular random identification and CHROMagar showed good agreement for C. albicans, C. tropicalis, C. dubliniensis and C. krusei identification

Agreement between RAPD, API20C AUX, CHROMagar Candida and microculture on oral Candida identification

measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. After this, agreement among methods using Kappa test was performed. Results: Agreement rates between RAPD and CHROMagar Candida, showed significant accuracy for C. albicans, C. tropicalis, C. dubliniensis and C. krusei (Kappa: 0.760, 0.640, 0.416 and 0.360, respectively, p0.05). The agreement was significant for all identified species when RAPD (OPE-18) and API 20C AUX (p<0.05). Comparing RAPD results with microculture, the highest agreement was for C. albicans (Kappa: 0.851 p<0.05) but no significant agreement for C. lusitaniae, C. krusei and C. guilliermondii was obtained (p<0.05). The agreement was significant for all identified species when RAPD (OPE-18) and API 20C AUX (p<0.05) were used. Critical levels of agreement between RAPD and microculture were observed when C. lusitaniae, C. krusei and C. guilliermondii were identified. Conclusions: API 20C AUX presented the best agreement with molecular random identification and CHROMagar showed good agreement for C. albicans, C. tropicalis, C. dubliniensis and C. krusei identification

Polymicrobial Leakage and Retention of MTA and Portland Cement in a Model of Apexification

Objective:To evaluate the sealing capacity and retention of apical barriers made with mineral trioxide aggregate (MTA) and Portland cement (PC). Material and Methods:Fifty-six bovine incisors were sectioned 8 mm above and 12 mm below the cement-enamel junction. The root canal was enlarged with a diamond drill to create a standard 2.5 mm diameter opening. Apical sheets of 5 mm thickness were placed using white MTA-Angelus or white PC. Fifteen samples of each material were exposed to human saliva in a dual chamber apparatus and casting was evaluated at 30 days. Samples without apical barriers and fully sealed samples were used as positive and negative controls (n = 3), respectively. Data were analyzed by Fisher's exact test (p0.05). Conclusion:Mineral trioxide aggregateand Portland cementapical barriers presented similar sealing ability and bond strength values