112 research outputs found
Développement de nouvelles stratégies de prévention et de résistance aux infections opportunistes chez l'Omble de fontaine (Salvelinus fontinalis)
Les bactĂ©ries peuvent exploiter quasiment toutes les niches Ă©cologiques, dont les organismes multicellulaires. Les relations hĂŽte-bactĂ©ries se classent sur une grande gamme dâinteractions allant du mutualisme au parasitisme. Dans ce contexte, lâobjectif thĂ©orique de mon doctorat est dâĂ©tudier les relations complexes existant entre un poisson, lâOmble de fontaine, et le cortĂšge microbien qui lâaccompagne. Cette espĂšce est la cible dâinfections opportunistes par des bactĂ©ries pathogĂšnes du genre Flavobacterium. Lâobjectif appliquĂ© visait Ă dĂ©velopper une approche alternative aux antibiotiques pour prĂ©venir et traiter les infections opportunistes. Le premier rĂ©sultat du projet a mis en lumiĂšre quâun stress hypoxique entraĂźnait une diminution des bactĂ©ries Ă potentiel bĂ©nĂ©fique provoquant une dysbiose, favorisant Ă son tour lâinfection par les pathogĂšnes. Nous avons donc ensuite isolĂ© des bactĂ©ries issues du microbiome pour tester leurs effets antagonistes vis-Ă -vis des pathogĂšnes F. columnare et F. psychophilum. Nous avons pu ainsi dĂ©couvrir sept candidats potentiels in vitro et tester un candidat qui sâest montrĂ© efficace par compĂ©tition par interfĂ©rence dans le traitement de la maladie de lâeau froide in vivo. Le dernier objectif de cette thĂšse consistait Ă tester la prĂ©sence de rĂ©gions gĂ©niques chez lâhĂŽte permettant le recrutement des bactĂ©ries bĂ©nĂ©fiques dans le microbiome. Nous avons donc analysĂ© le microbiome cutanĂ© de 86 individus dâune famille dâhybrides de deuxiĂšme gĂ©nĂ©ration. Nous avons observĂ© une forte variabilitĂ© interindividuelle et dĂ©terminĂ© trois rĂ©gions gĂ©niques impliquĂ©es dans la modulation de trois taxons bactĂ©riens dont Methylobacterium, qui est primordial dans le maintien de lâhomĂ©ostasie du microbiome. Tous nos rĂ©sultats appuient le rĂŽle du microbionte cutanĂ© dans la protection contre les infections opportunistes.Bacteria can colonize every ecological niche including multicellular organisms, due to their fast growth and their biochemical abilities, . Host-bacteria relationships are complex and range from parasitism to mutualism. Under this framework, my theoretical objective is to study the relationship complexity between brook charr and its microbial partners. This fish species is targeted by opportunistic infections from bacterial pathogens belonging to the Flavobacterium genus. My applied objective focuse on the development of alternative strategies to prevent and treat these infections without antibiotics. The first result of this project highlight that hypoxic stress induce a diminution of the potentially beneficial bacteria leading to a dysbiosis, which in turn triggers opportunistic infections. Then, we isolated bacteria from the microbiome to test their antagonistic effects on two pathogens Flavobacterium columnare and F. psychrophilum. We identify seven potential candidates in vitro, one tested in vivo and being effective to prevent cold-water disease via interference competition. The last objective is to test the existence of host genetic regions associated with the recruitment capacity of beneficial genera in the microbiome. To this end, we analyze the skin microbiome of 86 progenies issued from a dihybrid cross. A strong inter-individual variability is observed and we identify three genetic regions involved in the recruitment of three bacterial genera including Methylobacterium, which is very important to maintain microbiome homeostasis. Overall, the results demonstrate the role of skin microbiota in brook charr resistance against opportunistic infections
High order numerical schemes for transport equations on bounded domains
This article is an account of the NABUCO project achieved during the summer camp CEMRACS 2019 devoted to geophysical fluids and gravity flows. The goal is to construct finite difference approximations of the transport equation with nonzero incoming boundary data that achieve the best possible convergence rate in the maximum norm. We construct, implement and analyze the so-called inverse Lax-Wendroff procedure at the incoming boundary. Optimal convergence rates are obtained by combining sharp stability estimates for extrapolation boundary conditions with numerical boundary layer expansions. We illustrate the results with the Lax-Wendroff and O3 schemes
Dental Biofilm and Saliva Microbiome and Its Interplay with Pediatric Allergies
Little is known about the interplay and contribution of oral microorganisms to allergic
diseases, especially in children. The aim of the clinical study was to associate saliva and dental
biofilm microbiome with allergic disease, in particular with allergic asthma. In a single-center
study, allergic/asthmatic children (n = 15; AA-Chd; age 10.7 ± 2.9), atopic/allergic children (n = 16;
AT/AL-Chd; 11.3 ± 2.9), and healthy controls (n = 15; CON-Chd; age 9.9 ± 2.2) were recruited.
After removing adhering biofilms from teeth and collecting saliva, microbiome was analyzed by
using a 16s-rRNA gene-based next-generation sequencing in these two mediums. Microbiome
structure differed significantly between saliva and dental biofilms (ÎČ-diversity). Within the groups,
the dental biofilm microbiome of AA-Chd and AT/AL-Chd showed a similar microbial fingerprint
characterized by only a small number of taxa that were enriched or depleted (4) compared to the
CON-Chd, while both diseased groups showed a stronger microbial shift compared to CON-Chd,
revealing 14 taxa in AA-Chd and 15 taxa in AT/AL-Chd that were different. This could be the first
note to the contribution of dental biofilm and its metabolic activity to allergic health or disease
Staphylococcus massiliensis isolated from human blood cultures, Germany, 2017-2020
Clinical and laboratory data on newly described staphylococcal species is rare, which hampers decision-making when such
pathogens are detected in clinical specimens. Here, we describe Staphylococcus massiliensis detected in three patients at a
university hospital in southwest Germany. We report the discrepancy of microbiological fndings between matrix-assisted
laser desorption/ionization time-of-fight mass spectrometry, 16S-rRNA polymerase chain reaction, and whole-genome
sequencing for all three isolates. Our fndings highlight the diagnostic pitfalls pertinent to novel and non-model organisms
in daily microbiological practice, in whom the correct identifcation is dependent on database accuracy
Postoperative complications are associated with long-term changes in the gut microbiota following colorectal cancer surgery
Changes in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term changes in the gut microbiota and to correlate these changes with the clinical course of the patient. Methods: In total, stool samples of 62 newly diagnosed colorectal cancer patients undergoing primary tumor resection were analyzed by 16S-rDNA next-generation sequencing. Stool samples were collected preoperatively in order to determine the gut microbiome at baseline as well as at 6, 12, and 24 months thereafter to observe longitudinal changes. Postoperatively, the study patients were separated into two groups-patients who suffered from postoperative complications
Gut microbiome patterns correlate with higher postoperative complication rates after pancreatic surgery
Abstract Background Postoperative complications are of great relevance in daily clinical practice, and the gut microbiome might play an important role by preventing pathogens from crossing the intestinal barrier. The two aims of this prospective clinical pilot study were: (1) to examine changes in the gut microbiome following pancreatic surgery, and (2) to correlate these changes with the postoperative course of the patient. Results In total, 116 stool samples of 32 patients undergoing pancreatic surgery were analysed by 16S-rRNA gene next-generation sequencing. One sample per patient was collected preoperatively in order to determine the baseline gut microbiome without exposure to surgical stress and/or antibiotic use. At least two further samples were obtained within the first 10âdays following the surgical procedure to observe longitudinal changes in the gut microbiome. Whenever complications occurred, further samples were examined. Based on the structure of the gut microbiome, the samples could be allocated into three different microbial communities (A, B and C). Community B showed an increase in Akkermansia, Enterobacteriaceae and Bacteroidales as well as a decrease in Lachnospiraceae, Prevotella and Bacteroides. Patients showing a microbial composition resembling community B at least once during the observation period were found to have a significantly higher risk for developing postoperative complications (B vs. A, odds ratioâ=â4.96, pâ<â0.01**; B vs. C, odds ratioâ=â2.89, pâ=â0.019*). Conclusions The structure of the gut microbiome is associated with the development of postoperative complications
Satellite and in situ sampling mismatches: Consequences for the estimation of satellite sea surface salinity uncertainties
Validation of satellite sea surface salinity (SSS) products is typically based on comparisons with in-situ measurements at a few metersâ depth, which are mostly done at a single location and time. The difference in term of spatio-temporal resolution between the in-situ near-surface salinity and the two-dimensional satellite SSS results in a sampling mismatch uncertainty. The Climate Change Initiative (CCI) project has merged SSS from three satellite missions. Using an optimal interpolation, weekly and monthly SSS and their uncertainties are estimated at a 50 km spatial resolution over the global ocean. Over the 2016â2018 period, the mean uncertainty on weekly CCI SSS is 0.13, whereas the standard deviation of weekly CCI minus in-situ Argo salinities is 0.24. Using SSS from a high-resolution model reanalysis, we estimate the expected uncertainty due to the CCI versus Argo sampling mismatch. Most of the largest spatial variability of the satellite minus Argo salinity is observed in regions with large estimated sampling mismatch. A quantitative validation is performed by considering the statistical distribution of the CCI minus Argo salinity normalized by the sampling and retrieval uncertainties. This quantity should follow a Gaussian distribution with a standard deviation of 1, if all uncertainty contributions are properly taken into account. We find that (1) the observed differences between Argo and CCI data in dynamical regions (river plumes, fronts) are mainly due to the sampling mismatch; (2) overall, the uncertainties are well estimated in CCI version 3, much improved compared to CCI version 2. There are a few dynamical regions where discrepancies remain and where the satellite SSS, their associated uncertainties and the sampling mismatch estimates should be further validated
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