Confirmation of symmetrical distributions of clinical attachment loss and tooth loss in a homogeneous Mexican adult male population

Background/purpose To ascertain whether or not clinical attachment loss and tooth loss are present with similar severity and prevalence across the two sides of the mouth in a homogeneous sample of urban male adults. Materials and methods A cross-sectional study was carried out on 161 policemen (a largely homogeneous group in terms of ethnic background, socioeconomic status, sex, occupation, and medical/dental insurance) in Campeche, Mexico. Periodontal examinations were undertaken using the Florida Probe System in a dental chair by one trained and standardized examiner (kappa ≥ 0.60) to determine clinical attachment loss and tooth loss. We examined six sites in all teeth present in the mouth (a maximum of 168 sites, no third molars). Because of correlated data between observations, McNemar (for tooth loss) and Wilcoxon (for attachment loss) signed-rank tests were used to compare right and left sites within the same patient. Results The mean age was 38.4 ± 11.0 years. The mean number of teeth present was 24.4 ± 4.6; the mean number of periodontal sites/person was 146.7 ± 27.8. All P values were ≥ 0.05 (except for attachment loss in the upper first premolars), suggesting that there were no statistically significant differences between the right and left sides for the frequency of presentation of these two conditions. Conclusion Tooth loss and attachment loss measurements largely resemble each other on both sides of the mouth

“Today I Have Left My Armor at Home” Revisiting Jean Rhys’s Interwar Novels after the Ethical Turn

Within the critical interpretation that too often conflates life and fiction, the so-called "Jean Rhys woman" has resisted full assimilation by a feminist literary canon that advocates empowerment and agency. Browsing through Rhys's bibliography, it is interesting to note the considerable number of titles that suggest a commiserating attitude towards both the novelist and her female characters. Rhys's nomads, expatriates and zombielike figures have been analysed from the prism of postcolonial criticism as strongly inspired by the novelist's Caribbean background, their undecidable and precarious nature might be explained as part of what Butler has termed "certain exclusionary conceptions of who is normatively human". Other instances of abject corporealities can be found in Rhys's allusion to mannequins and inanimate bodies that elicited particular fascination in the interwar period, when huge numbers of injured soldiers acquired arms and legs prostheses, thus imposing new ways of thinking the body and its limits

Extreme Floods in Small Mediterranean Catchments: Long-Term Response to Climate Variability and Change

Climate change implies changes in the frequency and magnitude of flood events. The influence of climate variability on flooding was evaluated by an analysis of sedimentary (palaeofloods) and documentary archives. A 500-year palaeoflood record at Montlleó River (657 km2 in catchment area), eastern Spain, revealed up to 31 palaeofloods with a range of discharges of 20–950 m3 s−1, and with at least five floods exceeding 740–950 m3 s−1. This information contrasts with the available gauged flood registers (since year 1971) with an annual maximum daily discharge of 129 m3 s−1. Our palaeoflood dataset indicates flood cluster episodes at (1) 1570–1620, (2) 1775–1795, (3) 1850–1890, and (4) 1920–1969. Flood rich periods 1 and 3 corresponded to cooler than usual (about 0.3 °C and 0.2 °C) climate oscillations, whereas 2 and 4 were characterised by higher inter-annual climatic variability (floods and droughts). This high inter-annual rainfall variability increased over the last 150 years, leading to a reduction of annual maximum flow. Flood quantiles (>50 years) calculated from palaeoflood+gauged data showed 30%–40% higher peak discharges than those using only instrumental records, whereas when increasing the catchment area (1500 km2) the discharge estimation variance decreased to ~15%. The results reflect the higher sensitivity of small catchments to changes on flood magnitude and frequency due to climate variability whereas a larger catchment buffers the response due to the limited extent of convective storms. Our findings show that extended flood records provide robust knowledge about hazardous flooding that can assist in the prioritization of low-regret actions for flood-risk adaptation to climate change

Co-administration of human MSC overexpressing HIF-1α increases human CD34+ cell engraftment in vivo

Background: Poor graft function or graft failure after allogeneic stem cell transplantation is an unmet medical need, in which mesenchymal stromal cells (MSC) constitute an attractive potential therapeutic approach. Hypoxia-inducible factor-1α (HIF-1α) overexpression in MSC (HIF-MSC) potentiates the angiogenic and immunomodulatory properties of these cells, so we hypothesized that co-transplantation of MSC-HIF with CD34+ human cord blood cells would also enhance hematopoietic stem cell engraftment and function both in vitro and in vivo. Methods: Human MSC were obtained from dental pulp. Lentiviral overexpression of HIF-1α was performed transducing cells with pWPI-green fluorescent protein (GFP) (MSC WT) or pWPI-HIF-1α-GFP (HIF-MSC) expression vectors. Human cord blood CD34+ cells were co-cultured with MSC WT or HIF-MSC (4:1) for 72 h. Then, viability (Annexin V and 7-AAD), cell cycle, ROS expression and immunophenotyping of key molecules involved in engraftment (CXCR4, CD34, ITGA4, c-KIT) were evaluated by flow cytometry in CD34+ cells. In addition, CD34+ cells clonal expansion was analyzed by clonogenic assays. Finally, in vivo engraftment was measured by flow cytometry 4-weeks after CD34+ cell transplantation with or without intrabone MSC WT or HIF-MSC in NOD/SCID mice. Results: We did not observe significant differences in viability, cell cycle and ROS expression between CD34+ cells co-cultured with MSC WT or HIF-MSC. Nevertheless, a significant increase in CD34, CXCR4 and ITGA4 expression (p = 0.009; p = 0.001; p = 0.013, respectively) was observed in CD34+ cells co-cultured with HIF-MSC compared to MSC WT. In addition, CD34+ cells cultured with HIF-MSC displayed a higher CFU-GM clonogenic potential than those cultured with MSC WT (p = 0.048). We also observed a significant increase in CD34+ cells engraftment ability when they were co-transplanted with HIF-MSC compared to CD34+ co-transplanted with MSC WT (p = 0.016) or alone (p = 0.015) in both the injected and contralateral femurs (p = 0.024, p = 0.008 respectively). Conclusions: Co-transplantation of human CD34+ cells with HIF-MSC enhances cell engraftment in vivo. This is probably due to the ability of HIF-MSC to increase clonogenic capacity of hematopoietic cells and to induce the expression of adhesion molecules involved in graft survival in the hematopoietic niche

Will there be cold-related mortality in Spain over the 2021–2050 and 2051–2100 time horizons despite the increase in temperatures as a consequence of climate change?

Global warming is resulting in an increase in temperatures which is set to become more marked by the end of the century and depends on the accelerating pace of greenhouse gas emissions into the atmosphere. Yet even in this scenario, so-called “cold waves” will continue to be generated and have an impact on health. Objectives: This study sought to analyse the impact of cold waves on daily mortality at a provincial level in Spain over the 2021–2050 and 2051–2100 time horizons under RCP4.5 and RCP 8.5 emission scenarios, on the basis of two hypotheses: (1) that the cold-wave definition temperature (T threshold) would not vary over time; and, (2) that there would be a variation in T threshold