牛尾椎椎間板におけるインスリン様成長因子-1(IGF-1)の自己分泌機構及びIGF-1が椎間板のプロテオグリカン合成に与える影響

The present study was undertaken to investigate the effect of IGF-1 on proteoglycan (PG) synthesis and the autocrine/paracrine mechanisms involving insulin-like growth factor-1 (IGF-1) in the bovine coccygeal intervertebral disc.IGF-1 stimulated PG synthesis in cultured cells of nucleus pulposus of bovine intervertebral discs in a dose-dependent manner, and the effect was inhibited by an anti-IGF-1 monoclonal antibody.Insitu hybridization histochemistry revealed the expression of IGF-1 mRNA in the cultured cells, and IGF-1 production in these cells was demonstrated by radioimmunoassay.IGF-1 receptor in the cultured cells was also immunohistochemically demonstrated.The Scatchard analysis using an ^I-IGF-1 binding assay showed that the cells cultured in monolayer had a single type of IGF-1 receptor, whose affinity and number were estimated to be 7.38 x 10^8/M and 9.27 x 10^4/cell, respectively. These results suggest that IGF-1 stimulates PG synthesis in cells of the nucleus pulposus, and that these cells in culture have an IGF-1 autocrine/paracrine mechanism.The expressions of IGF-1 mRNA and IGF-1 receptor in disc tissue were greater in cells of the nucleus pulposus of fetal bovine intervertebral discs than in those of the adult discs.These findings suggest that the autocrine/paracrine IGF-1 action is more active in cells of young nucleus pulposus than in cells of matured subjects.富山医科薬科大学・博士(医学)・甲第240号・長田龍介・1996/3/8富山医科薬科大

Active Voluntary Contraction of the Ruptured Muscle Tendon during the Wide-awake Tendon Reconstruction

Purpose:. The purpose of this study was to measure the active voluntary contraction distances of ruptured musculotendinous units during wide-awake tendon reconstruction surgery and to investigate the relationships between active contraction distance and the passive distraction distance and the time elapsed before reconstruction. Methods:. The passive distraction distance and active contraction distance of 36 tendons of 22 patients who underwent forearm tendon transfer or tendon graft during wide-awake surgery were measured. Results:. The passive distraction distance was significantly related to the active contraction distance of the ruptured musculotendinous unit (r = 0.60, P < 0.05). The passive distraction distance accounted for approximately half of the total excursion of the musculotendinous unit. There was no significant correlation between each distance and the time elapsed before reconstruction. Conclusions:. The passive distraction distance was significantly related to the active contraction distance of the ruptured musculotendinous unit in vivo during wide-awake surgery. Active musculotendinous unit contraction distance may be a useful index to evaluate ruptured musculotendinous unit degeneration and donor muscle function in standard tendon reconstruction. This study suggested that the advantages of the wide-awake approach were not just as a clinical tool but also as an in vivo research tool