Interdyscyplinarna opieka nad chorymi po zabiegach z zakresu chirurgii szczękowo-twarzowej

Wstęp: Zabiegi z zakresu chirurgii czaszkowo-szczękowo-twarzowej ze względu na swoją specyfikę mogą prowadzić do znacznych upośledzeń czynnościowych. Zniekształcenia pooperacyjne i pourazowe twarzy oraz wady ortognatyczne zaburzają estetykę i harmonię rysów twarzy, utrudniając choremu wyrażanie emocji i porozumiewanie się z otoczeniem. Dlatego priorytetem jest interdyscyplinarna opieka przedi pooperacyjna. Zespół lekarzy, fizjoterapeutów, logopedów, foniatrów, dietetyków oraz psychologów ma przywrócić pacjenta do życia w społeczeństwie w zakresie najbardziej zbliżonym do tego sprzed choroby. Materiał i metody: Badaniem objęto 132 pacjentów Przyklinicznego Zespołu Rehabilitacyjnego Kliniki Chirurgii Czaszkowo-Szczękowo-Twarzowej SUM w Katowicach w latach 2020–2022. Wiek chorych wahał się w zakresie 20–70 lat, średnia wieku wynosiła 44,02. Wśród badanych osób było 71 kobiet (53,8%); 45,5% pacjentów stanowiły osoby po urazach twarzoczaszki, 20,5% osoby z dysfunkcjami stawów skroniowo-żuchwowych, 18,9% chorzy onkologiczni, a 15,2% pacjenci z innymi schorzeniami. Chorzy poddani zabiegom uczestniczyli w rehabilitacji przed- i pooperacyjnej, która odbywała się w trakcie hospitalizacji oraz ambulatoryjnie. Wykonywano zabiegi rehabilitacyjne z zakresu nowoczesnej terapii ruchowej, terapii oddechowej, manualnego drenażu limfatycznego, manualnej terapii mięśniowo-powięziowej oraz procedury wspomagające, jak: suche igłowanie, kinesiotaping i techniki fizjoterapeutyczne. Pacjenci korzystali dodatkowo z pomocy dietetyka, psychologa oraz logopedy. Wyniki: Skuteczność przeprowadzonej rehabilitacji uzyskano w około 90% w zakresie zmniejszenia obrzęku, terapii blizn oraz w działaniu przeciwbólowym. Wzrost odwodzenia żuchwy notowano u 79,2% pacjentów. Porównanie pacjentów po przebytym urazie twarzoczaszki i z nowotworem tej okolicy wskazuje na uzyskanie gorszych wyników u pacjentów onkologicznych oraz występowanie u nich dodatkowych problemów z mową i oddychaniem. Wnioski: Wielospecjalistyczna terapia jest nieodłącznym elementem uzupełniającym leczenie chirurgiczne. Uzyskane wyniki wskazują na potrzebę przeprowadzenia dalszych badań klinicznych na poszczególnych grupach chorych, aby dać możliwość opracowania algorytmów interdyscyplinarnego postępowania z chorym na oddziale chirurgii szczękowo-twarzowej

Cancer incidence and mortality in Greater Poland Province in 2006 – Report

Greater Poland is a region of high cancer risk in Poland. Standardized rates for cancer incidence among man and woman are the highest in Poland. Standardized rates for cancer deaths are also very high (4th place for men and 2nd for woman).When analysing the absolute numbers of cases and deaths among men in Greater Poland for last eight years, we observe increase of cases number by 16,14% and deaths by 10,85%.Most common cancer incidence sites in Greater Poland for man are: lung, colorectum and prostate. Among woman breast, colorectum and lung.Most common cancer deaths sites in men are: lung, colorectum and stomach. Among woman breast, lung and colorectum.When analysing standardized rates for malignant tumors morbidity and mortality among men and women, we see clearly that increase of number of new cancer cases and deaths is determined first of all by ageing in Greater Poland population.Lung cancer in Greater Poland is situated in first three most common cancer sites by incidence and mortality. Region has a medium lung cancer incidence and mortality compare to other voivodships. In analyzed period (1999–2006) lung cancer incidence and mortality among men has decreased. Unfortunately the same trend is not observed among women.High breast cancer incidence is very typical for Greater Poland. Analysis of standardized rates shows that Greater Poland is on 3rd place by breast cancer incidence and on the 4th place by breast cancer mortality. The most important and at the same time the cheapest method to eliminate tumors is prophylactics and screening

Zarządzanie i handel zagraniczny w małych i średnich przedsiębiorstwach w warunkach integracji europejskiej: materiały z konferencji

Z przedmowy: "Integracja europejska to proces łączenia, scalania się odrębnych ekonomicznie, społecznie, kulturowo gospodarek europejskich krajów. Proces integracji prowadzi do istotnych przekształceń w sferze gospodarki, strategiach organizacji i funkcjonowania przedsiębiorstw, handlu międzynarodowym, działalności marketingowej, strukturach organizacyjnych i mechanizmach ekonomicznych przedsiębiorstw i instytucji działających w krajach integrujących się. Proces integracji to w praktyce proces dostosowywania się struktur gospodarczych; tworzenia związków kooperacyjno-produkcyjnych; powstawania trwałych więzi ekonomicznych między przedsiębiorstwami integrujących się krajów a więc proces kształtowania jednolitego obszaru gospodarczego z odrębnych a często także wzajemnie konkurencyjnych krajów, gospodarek, regionów, gałęzi, branż. Proces międzynarodowej integracji gospodarczej to w dużej mierze proces tworzenia komplementamości przedsiębiorstw i instytucji, komplementamości międzygałęziowej i wewnątrz gałęziowej, w produkcji i wymianie jak też kształtowanie niezbędnej infrastruktury technicznej i ekonomicznej umożliwiającej tworzenie sytemu trwałych powiązań gospodarczych między poszczególnymi krajami."(...

Switching handedness : fMRI study of hand motor control in right-handers, left-handers and converted left-handers

The purpose of this study was to investigate the differences in the brain organization of motor control in left- and righthanders and to study whether early left-to-right handwriting switch changes the cortical representation of finger movements in the left and right hemispheres. Echo-planar MR imaging was performed in 52 subjects: consistent right-handers (RH), consistent left-handers (LH), and subjects who had been forced at an early age to switch their left-hand preferences toward the right side. The scanning was performed during simple (flexion/extension of the index finger) and complex (successive finger-thumb opposition) tasks. Subjects performed the tasks using both the preferred and non-preferred hand. In righthanders, there was a general predominance of left-hemisphere activation relative to right hemisphere activation. In left-handers this pattern was reversed. The switched subjects showed no such volumetric asymmetry. Increasing levels of complexity of motor activity resulted in an increase in the volume of consistently activated areas and the involvement of the ipsilateral in addition to contralateral activations. In both right-and left-handers, movements of the preferred hand activated mainly the contralateral hemisphere, whereas movements of the non-preferred hand resulted in a more balanced pattern of activation in the two hemispheres, indicating greater involvement of the ipsilateral activations. Overall, this study shows that in both left-and right-handed subjects, the preferred hand is controlled mainly by the hemisphere contralateral to that hand, whereas the non-preferred hand is controlled by both hemispheres. The switched individuals share features of both left-handers and right-handers regarding their motor control architectures

Heurs et malheurs de la décentralisation en France, de la fin de l’Ancien Régime à la troisième République

20-hydroxyecdysone (20E) and juvenile hormone (JH) signaling pathways interact to regulate insect development. Recently,two proteins, a calponin-like Chd64 and immunophilin FKBP39 have been found to play a pivotal role in the cross-talkbetween 20E and JH, although the molecular basis of interaction remains unknown. The aim of this work was to identify thestructural features that would provide understanding of the role of Chd64 in multiple and dynamic complex that cross-linksthe signaling pathways. Here, we demonstrate the results of in silico and in vitro analyses of the structural organization ofChd64 from Drosophila melanogaster and its homologue from Tribolium castaneum. Computational analysis predicted theexistence of disordered regions on the termini of both proteins, while the central region appeared to be globular, probablycorresponding to the calponin homology (CH) domain. In vitro analyses of the hydrodynamic properties of the proteinsfrom analytical size-exclusion chromatography and analytical ultracentrifugation revealed that DmChd64 and TcChd64 hadan asymmetrical, elongated shape, which was further confirmed by small angle X-ray scattering (SAXS). The Kratky plotindicated disorderness in both Chd64 proteins, which could possibly be on the protein termini and which would give rise tospecific hydrodynamic properties. Disordered tails are often involved in diverse interactions. Therefore, it is highly possiblethat there are intrinsically disordered regions (IDRs) on both termini of the Chd64 proteins that serve as platforms formultiple interaction with various partners and constitute the foundation for their regulatory function

Brain correlates of right-handedness

Recent development of neuroimaging techniques has opened new possibilities for the study of the relation between handedness and the brain functional architecture. Here we report fMRI measurements of dominant and non-dominant hand movement representation in 12 right-handed subjects using block design. We measured possible asymmetry in the total volume of activated neural tissue in the two hemispheres during simple and complex finger movements performed either with the right hand or with the left hand. Simple movements consisted in contraction/extension of the index finger and complex movements in successive finger-thumb opposition from little finger to index finger. A general predominance of left-hemisphere activation relative to right hemisphere activation was found. Increasing the complexity of the motor activity resulted in an enlargement of the volume of consistently activated areas and greater involvement of ipsilateral areas, especially in the left hemisphere. Movements of the dominant hand elicited large contralateral activation (larger than movements of the non-dominant hand) and relatively smaller ipsilateral activation. Movements of the non-dominant hand resulted in a more balanced pattern of activation in the two hemispheres, due to relatively greater ipsilateral activation. This suggests that the dominant (right) hand is controlled mainly by the contralateral (left) hemisphere, whereas the non-dominant hand is controlled by both left and right hemispheres. This effect is especially apparent during execution of complex movements. The expansion of brain areas involved in motor control in the hemisphere contralateral to the dominant hand may provide neural substrate for higher efficiency and a greater motor skill repertoire of the preferred hand

Hydrodynamic properties of DmChd64 and TcChd64.

<p>(A) Analytical size-exclusion chromatography of DmChd64 (dashed line) and TcChd64 (solid lane). Experiments were performed on a Superdex 200 10/300 GL column equilibrated with 50 mM Na₂HPO4, 150 mM NaCl, pH 7 at room temperature and at a flow rate of 0.5 ml/min; an injection volume of 0.1 ml was used and the protein concentration was 1 mg/ml. The inset shows the calibration curve determined using standard proteins (black dots). The black square corresponds to DmChd64 and the grey dot to TcChd64. (B), (D) Sedimentation velocity analytical ultracentrifugation analysis. Superposition of the distribution of sedimentation coefficients, c(s) derived via SEDFIT from SV data for DmChd64 in two different concentrations (B) and for TcChd64 in two different concentrations (D) measured using absorbance optics at 280 nm during an SV experiment at 30 000 rpm at 20°C, standardised to water at 20°C. (C), (E) Example of the sedimentation profile of DmChd64 at 0.53 mg/ml (C) and TcChd64 at 0.6 mg/ml (E). Superposition of selected experimental (circles) and fitted (solid lines) SV profiles corrected for all systematic noises, with an rmsd of 0.004531 for DmChd64 and 0.003496 for TcChd64 indicating a good fit of the SV data. The inset shows the superposition of the residuals between the experimental and fitted curves.</p

3D structure predictions of DmChd64 and TcChd64.

<p>The model of the DmChd64 structure is presented on panel (A) and TcChd64 on (B). Structure conformation was generated by web-based tool ITASSER <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096809#pone.0096809-Roy1" target="_blank">[57]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096809#pone.0096809-Zhang1" target="_blank">[58]</a> and results were visualised by PyMOL <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096809#pone.0096809-DeLano1" target="_blank">[59]</a>. N-terminal tails are highlighted in blue and C-terminals are in red. The CH domain is marked in grey, conserved C residues are yellow. The arrows point to non-conserved C residues marked in orange.</p

Schematic illustration and purification of recombinant DmChd64 and TcChd64.

<p>(A) Schematic illustration of recombinant DmChd64 and TcChd64. Recombinant constructs for DmChd64 and TcChd64 were prepared. DmChd64/TcChd64was N-terminally tagged with the Strep II-tag, and C-terminally tagged with the 8×His-tag. DmChd64/TcChd64 was N-terminally tagged with the Strep II-tag and DmChd64/TcChd64 was C-terminally tagged with the 8×His-tag. (B) Coomasie Brilliant Blue R-250-stained SDS-PAGE analysis of the expression and purification procedure of DmChd64. Lane 1, molecular mass standards; lane 2 and 3, whole cell extracts from bacterial cells containing the pQE80L-SXH plasmid with the cDNA insert (pQE80L-SXH/DmChd64) harvested before (T_NI) and 3 h after expression induction with IPTG (T_{3 h}); lane 4, soluble protein fractions obtained after cell lysis (Sup); lane 5, the flow-through (FT) fraction obtained from the Co²⁺-TALON column; lane 6, protein fractions containing impurities washed (W) from the Co²⁺-TALON column using buffer A; lane 7, protein fractions containing impurities eluted during the imidazole gradient sub-step using buffer A and B solutions containing 20 mM imidazole (Im₂₀); lane 8, pooled fractions eluted from Co²⁺-TALON resin with buffer B containing 200 mM imidazole (Im₂₀₀); lane 9, protein fractions containing impurities washed (W) from the Strep-Trap HP column using buffer C; lane 10, pooled fractions eluted from the Strep-TrapHP column with buffer D containing 2.5 mM desthiobiotin (D_2.5); lane 11, pooled fractions of DmChd64 from the Superdex 200 10/300 GL column (S). (C) Coomasie Brilliant Blue R-250-stained SDS-PAGE analysis of the expression and purification procedure of TcChd64. Lane 1, molecular mass standards; lane 2 and 3, whole cell extracts from bacterial cells containing the pQE80L-XH plasmid with the cDNA insert (pQE80L-XH/TcChd64) harvested before (T_NI) and 3 h after expression induction with IPTG (T_{3 h}); lane 4, soluble protein fractions obtained after cell lysis (Sup); lane 5, the flow-through (FT) fraction obtained from the Co²⁺-TALON column; lane 6, protein fractions containing impurities washed (W) from the Co²⁺-TALON column using buffer E; lane 7, protein fractions containing impurities eluted during the imidazole gradient sub-step using buffer E and F solutions containing 20 mM imidazole (Im₂₀); lane 8, pooled fractions eluted from the Co²⁺-TALON resin with buffer F containing 200 mM imidazole (Im₂₀₀); lane 9, protein fractions containing impurities from the first peak from tandem connected Superdex 75 10/300 GL columns (S₁)_; lane 10, pooled fractions of TcChd64 from the two tandem Superdex 75 10/300 GL columns (S₂).</p