29,451 research outputs found
Stimulation-dependent uptake of an extracellular marker to subcellular fractions of isolated neurohypophysial tissue
Optimal land cover mapping and change analysis in northeastern oregon using landsat imagery.
Abstract The necessity for the development of repeatable, efficient, and accurate monitoring of land cover change is paramount to successful management of our planet’s natural resources. This study evaluated a number of remote sensing methods for classifying land cover and land cover change throughout a two-county area in northeastern Oregon (1986 to 2011). In the past three decades, this region has seen significant changes in forest management that have affected land use and land cover. This study employed an accuracy assessment-based empirical approach to test the optimality of a number of advanced digital image processing techniques that have recently emerged in the field of remote sensing. The accuracies are assessed using traditional error matrices, calculated using reference data obtained in the field. We found that, for single-time land cover classification, Bayes pixel-based classification using samples created with scale and shape segmentation parameters of 8 and 0.3, respectively, resulted in the highest overall accuracy. For land cover change detection, using Landsat-5 TM band 7 with a change threshold of 1.75 standard deviations resulted in the highest accuracy for forest harvesting and regeneration mapping
Double-discharge copper-vapor laser
Power supply for discharge pulses consists of two capacitors that are made to discharge synchronously with adjustable time intervals. First pulse is switched with hydrogen thyratron, and second by spark gap. Lasing action peaks for appropriate combination of these two parameters
Nonlinear optics in Xe-filled hollow-core PCF in high pressure and supercritical regimes
Supercritical Xe at 293 K offers a Kerr nonlinearity that can exceed that of
fused silica while being free of Raman scattering. It also has a much higher
optical damage threshold and a transparency window that extends from the UV to
the infrared. We report the observation of nonlinear phenomena, such as
self-phase modulation, in hollow-core photonic crystal fiber filled with
supercritical Xe. In the subcritical regime, intermodal four-wave-mixing
resulted in the generation of UV light in the HE12 mode. The normal dispersion
of the fiber at high pressures means that spectral broadening can clearly
obtained without influence from soliton effects or material damage
p-Wave stabilization of three-dimensional Bose-Fermi solitons
We explore bright soliton solutions of ultracold Bose-Fermi gases, showing
that the presence of p-wave interactions can remove the usual collapse
instability and support stable soliton solutions that are global energy minima.
A variational model that incorporates the relevant s- and p-wave interactions
in the system is established analytically and solved numerically to probe the
dependencies of the solitons on key experimental parameters. Under attractive
s-wave interactions, bright solitons exist only as meta-stable states
susceptible to collapse. Remarkably, the presence of repulsive p-wave
interactions alleviates this collapse instability. This dramatically widens the
range of experimentally-achievable soliton solutions and indicates greatly
enhanced robustness. While we focus specifically on the boson-fermion pairing
of 87Rb and 40K, the stabilization inferred by repulsive p-wave interactions
should apply to the wider remit of ultracold Bose-Fermi mixtures.Comment: 9 pages, 6 figure
Modified protein expression in the tectorial membrane of the cochlea reveals roles for the striated sheet matrix
The tectorial membrane (TM) of the mammalian cochlea is a complex extracellular matrix which, in response to acoustic stimulation, displaces the hair bundles of outer hair cells (OHCs), thereby initiating sensory transduction and amplification. Here, using TM segments from the basal, high-frequency region of the cochleae of genetically modified mice (including models of human hereditary deafness) with missing or modified TM proteins, we demonstrate that frequency-dependent stiffening is associated with the striated sheet matrix (SSM). Frequency-dependent stiffening largely disappeared in all three TM mutations studied where the SSM was absent either entirely or at least from the stiffest part of the TM overlying the OHCs. In all three TM mutations, dissipation of energy is decreased at low (<8 kHz) and increased at high (>8 kHz) stimulus frequencies. The SSM is composed of polypeptides carrying fixed charges, and electrostatic interaction between them may account for frequency-dependent stiffness changes in the material properties of the TM. Through comparison with previous in vivo measurements, it is proposed that implementation of frequency-dependent stiffening of the TM in the OHC attachment region facilitates interaction among tones, backward transmission of energy, and amplification in the cochlea
Fusion of neurohypophyseal membranes in vitro
Freeze cleaving electron microscopy has shown that fusion of isolated secretory vesicles from bovine neurohypophyses was induced by Ca
2+ in micromolar concentrations. Mg
2+ and Sr
2+ were ineffective. Mg
2+ inhibited Ca
2+-induced fusion.
In suspensions containing secretory vesicles as well as sheets of cell membrane, release of vasopressin parallel to intervesicular fusion of secretory vesicles with sheets of cell membrane was observed after exposure to Ca
2+. Mg
2+ and Sr
2+ were ineffective in replacing Ca
2+ as trigger for fusion or vasopressin release.
Intervesicular fusion and exocytotic profiles were observed when isolated neurohypophyses or neurosecretosome were exposed to cold
Effects of marine reserves in the context of spatial and temporal variation:an analysis using Bayesian zero-inflated mixed models
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