Versatile Potentiostat with Optional Computer Control

A versatile potentiostat which can supply a maximum of 125 ma is described. The potentiostat uses readily available electronic components and an interface is detailed which allows the potentiostat optional computer control

Complexity of Fetal Movement Detection Using a Single Doppler Ultrasound Transducer

The objective of this paper is to discuss the complexity of fetal movement detection encountered during development and implementation of an automated single Doppler ultrasonic transducer based instrument. The single transducer instrument was intended to better quantify the duration, velocity, and magnitude of fetal movements. A Corometrics Model 116 fetal heart rate monitor was modified, and a fetal movement detection algorithm (Russell Algorithm) was developed to detect fetal movements on one and two (data fusion) transducers. A Hewlett-Packard (HP) M-1350-A fetal monitor and the Russell Algorithm were used to detect and record fetal movements concurrently on sixty patients between the gestation ages of31 to 41 weeks. Using a computer-controlled SVHS PC-VCR, the instrumental detection of fetal movements was time-linked with real-time video ultrasound. This allowed the fetal movements to be scored by expert examiners on a second-per-second basis. A total of 52,478 seconds of fetal movements was scored using this system. Neither system could accurately define the entire duration, velocity, or magnitude of the fetal movements as detected by real-time ultrasound. The complexity of detecting fetal movements using only one transducer has many shortcomings, such as: the amplitude of the returning Doppler signal, the small area of the fetus monitored by a single transducer, the position of the fetus, the type and variety of fetal movements, and material size and shape

Complexity of Fetal Movement Detection Using a Single Doppler Ultrasound Transducer

The objective of this paper is to discuss the complexity of fetal movement detection encountered during development and implementation of an automated single Doppler ultrasonic transducer based instrument. The single transducer instrument was intended to better quantify the duration, velocity, and magnitude of fetal movements. A Corometrics Model 116 fetal heart rate monitor was modified, and a fetal movement detection algorithm (Russell Algorithm) was developed to detect fetal movements on one and two (data fusion) transducers. A Hewlett-Packard (HP) M-1350-A fetal monitor and the Russell Algorithm were used to detect and record fetal movements concurrently on sixty patients between the gestation ages of31 to 41 weeks. Using a computer-controlled SVHS PC-VCR, the instrumental detection of fetal movements was time-linked with real-time video ultrasound. This allowed the fetal movements to be scored by expert examiners on a second-per-second basis. A total of 52,478 seconds of fetal movements was scored using this system. Neither system could accurately define the entire duration, velocity, or magnitude of the fetal movements as detected by real-time ultrasound. The complexity of detecting fetal movements using only one transducer has many shortcomings, such as: the amplitude of the returning Doppler signal, the small area of the fetus monitored by a single transducer, the position of the fetus, the type and variety of fetal movements, and material size and shape

Gal4 turnover and transcription activation

Growing evidence supports the notion that proteasome-mediated destruction of transcriptional activators can be intimately coupled to their function. Recently, Nalley et al. challenged this view by reporting that the prototypical yeast activator Gal4 does not dynamically associate with chromatin, but rather 'locks in' to stable promoter complexes that are resistant to competition. Here we present evidence that the assay used to reach this conclusion is unsuitable, and that promoter-bound, active Gal4 is indeed susceptible to competition in vivo. Our data challenge the key evidence that Nalley et al. used to reach their conclusion, and indicate that Gal4 functions in vivo within the context of dynamic promoter complexes

Pennsylvania Folklife Vol. 29, No. 3

• Shuler Family Correspondence • Vestiges of the Markley Family • 30 Years of the Kutztown Folk Festival: A Photo Essay • The Rural Village • Father of the Fraternity: Christopher Schlegel and Rosicrucianism • A Lexical Comparison of Two Sister Languages: Pennsylvania German and Yiddish • Aldes un Neieshttps://digitalcommons.ursinus.edu/pafolklifemag/1087/thumbnail.jp

Linking Activity, Nutrition, and Child Health (Launch): Protocol for a Longitudinal Cohort Study of Children as They Develop From Infancy to Preschool Age

Background Physical activity is known to provide important health benefits in children ages 3 years and above, but little is known about the effects of physical activity on health in very young children under age 3. LAUNCH (Linking Activity, Nutrition, and Child Health) is a study designed to expand the body of knowledge on development of physical activity behavior and associations between physical activity and other health characteristics as children transition from infancy to preschool age. Methods Physical activity and sedentary behavior will be measured objectively in young children over a period of 30 months. Each child will complete a measurement protocol at 6, 12, 18, 24, 30 and 36 months of age. The following factors will be measured at each time point: physical activity, sedentary behavior, anthropometric characteristics, and motor developmental status. Objectively-measured sleep behavior will be included as an optional component of the protocol. Parents will provide information on demographic factors, parenting behaviors, home and childcare characteristics, and the child’s dietary and sleep behaviors. Discussion LAUNCH will employ a longitudinal study design and objective measures of physical activity, sedentary behavior and sleep in examining developmental trends for those characteristics in children between the ages of 6 and 36 months. Associations among physical activity, sedentary behavior, sleep, and weight status will be examined. Findings will inform public health guidance and intervention strategies for very young children

Communications Biophysics

Contains research objectives and reports on six research projects split into three sections.National Institutes of Health (Grant 5 P01 NS13126-07)National Institutes of Health (Training Grant 5 T32 NS07047-05)National Institutes of Health (Training Grant 2 T32 NS07047-06)National Science Foundation (Grant BNS 77-16861)National Institutes of Health (Grant 5 R01 NS1284606)National Institutes of Health (Grant 5 T32 NS07099)National Science Foundation (Grant BNS77-21751)National Institutes of Health (Grant 5 R01 NS14092-04)Gallaudet College SubcontractKarmazin Foundation through the Council for the Arts at M.I.T.National Institutes of Health (Grant 1 R01 NS1691701A1)National Institutes of Health (Grant 5 R01 NS11080-06)National Institutes of Health (Grant GM-21189

Limits to Rest-Frame Ultraviolet Emission From Far-Infrared-Luminous z~6 Quasar Hosts

We report on a Hubble Space Telescope search for rest-frame ultraviolet emission from the host galaxies of five far-infrared-luminous $z\simeq{}6$ quasars and the $z=5.85$ hot-dust free quasar SDSS J0005-0006. We perform 2D surface brightness modeling for each quasar using a Markov-Chain Monte-Carlo estimator, to simultaneously fit and subtract the quasar point source in order to constrain the underlying host galaxy emission. We measure upper limits for the quasar host galaxies of $m_J>22.7$ mag and $m_H>22.4$ mag, corresponding to stellar masses of $M_\ast<2\times10^{11}M_\odot$. These stellar mass limits are consistent with the local $M_{\textrm{BH}}$-$M_\ast$ relation. Our flux limits are consistent with those predicted for the UV stellar populations of $z\simeq6$ host galaxies, but likely in the presence of significant dust ($\langle A_{\mathrm{UV}}\rangle\simeq 2.6$ mag). We also detect a total of up to 9 potential $z\simeq6$ quasar companion galaxies surrounding five of the six quasars, separated from the quasars by 1.4''-3.2'', or 8.4-19.4 kpc, which may be interacting with the quasar hosts. These nearby companion galaxies have UV absolute magnitudes of -22.1 to -19.9 mag, and UV spectral slopes $\beta$ of -2.0 to -0.2, consistent with luminous star-forming galaxies at $z\simeq6$. These results suggest that the quasars are in dense environments typical of luminous $z\simeq6$ galaxies. However, we cannot rule out the possibility that some of these companions are foreground interlopers. Infrared observations with the James Webb Space Telescope will be needed to detect the $z\simeq6$ quasar host galaxies and better constrain their stellar mass and dust content.Comment: 22 pages, 13 figures. Accepted for publication in Ap

JWST NIRCam Photometry: A Study of Globular Clusters Surrounding Bright Elliptical Galaxy VV 191a at z=0.0513

James Webb Space Telescope NIRCam images have revealed 443 globular cluster (GC) candidates around the $z=0.0513$ elliptical galaxy VV 191a. NIRCam broadband observations are made at 0.9-4.5 $\mu$m using filters F090W, F150W, F356W, and F444W. Using photometry, the data is analyzed to present color-magnitude diagrams (CMDs) that suggest a fairly uniform population of GCs. Color histograms show a unimodal color distribution that is well fit by a single Gaussian, using color to primarily trace the metallicity. The findings show the sample's globular cluster luminosity function (GCLF) does not reach the turnover value and is, therefore, more luminous than what is typically expected, with an absolute AB magnitude, $M_{F090W} = -8.70$ mag, reaching within nearly one magnitude of the classical turnover value. We attribute this to the completeness in the sample. Models show that the mass estimate of the GCs detected tends to be more massive, reaching upward of $\simeq 10^7 M_{\odot}$. However, the results show that current GC models do not quite align with the data. We find that the models appear to be bluer than the JWST data in the reddest (F356W-F444W) filters and redder than the data in the bluest (F090W-F150W) filters and may need to be revised to improve the modeling of near-IR colors of old, metal-poor stellar populations.Comment: 11 pages, 7 figure

Ovine pedomics : the first study of the ovine foot 16S rRNA-based microbiome

We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing. Three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. The sheep were classified as having either healthy interdigital skin (H), interdigital dermatitis (ID) or virulent footrot (VFR). The ovine interdigital skin bacterial community varied significantly by flock and clinical condition. The diversity and richness of operational taxonomic units was greater in tissue from sheep with ID than H or VFR affected sheep. Actinobacteria, Bacteriodetes, Firmicutes and Proteobacteria were the most abundant phyla comprising 25 genera. Peptostreptococcus, Corynebacterium and Staphylococcus were associated with H, ID and VFR respectively. Sequences of Dichelobacter nodosus, the causal agent of ovine footrot, were not amplified due to mismatches in the 16S rRNA universal forward primer (27F). A specific real time PCR assay was used to demonstrate the presence of D. nodosus which was detected in all samples including the flock with no signs of ID or VFR. Sheep with ID had significantly higher numbers of D. nodosus (104-109 cells/g tissue) than those with H or VFR feet