29 research outputs found
Cannabinoid receptors expression in bone marrow trephine biopsy of chronic lymphocytic leukaemia patients treated with purine analogues
Background: Cannabinoid receptors CB1 and CB2 are part the endocannabinoid system that plays an important role in the process of proliferation and apoptosis of different neoplastic cells. B-cell chronic lymphocytic leukaemia is one of the diseases in which these processes are altered. Aim: The aim of our study was the assessment of cannabinoid receptor expression on the B-lymphocytes in bone marrow trephine biopsy from leukaemic patients at diagnosis and after purine analogue treatment. Methods: The biopsy was taken routinely and standard immunohistochemical staining procedure for paraffin embedded sections was applied. The cannabinoid receptors were detected using specific primary polyclonal antibody anti-CB1 and anti-CB2. Additionally, an existence of cannabinoid receptors was confirmed by flow cytometry. Results: The results showed that the expression of CB1 receptor on the surface of neoplastic cells was lower than that of CB2 (17.0 Β± 3.1% and 92.1 Β± 1.7% respectively, p < 0.001). Nine of the patients responded to applied treatment with a reduction in leukaemic infiltration (77.2 Β± 6.9% to 30.2 Β± 6.5%, p = 0.007) and CB1 receptor expression (24.4 Β± 4.8% to 8.6 Β± 2.9%, p = 0.01), but there was no change in CB2 expression (91.7 Β± 2.7% vs 90.9 Β± 2.8%, p = 0.69). Four patients without remission expressed even greater number of the receptors. In all of the cases both cannabinoid receptor types antibodies gave positive reaction. Furthermore, the existence of cannabinoid receptors on neoplastic lymphocytes was confirmed by flow cytometry. Conclusion: The study provides original evidence for the existence of cannabinoid receptors on B-lymphocytes in chronic lymphocytic leukaemia patients. The receptors are thought to be a new structure that can modify the course of the disease and may be considered as a new target in leukaemia treatment.ΠΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅: ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΡ ΠΊΠ°Π½Π½Π°Π±ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² CB1 ΠΈ CB2 ΡΠ² Π»ΡΡΡΡΡ ΡΠ°ΡΡΡΡ ΡΠΈΡΡΠ΅ΠΌΡ ΡΠ½Π΄ΠΎΠΊΠ°Π½Π½Π°Π±ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ², ΠΊΠΎΡΠΎΡΠ°Ρ ΠΈΠ³ΡΠ°Π΅Ρ Π²Π°ΠΆΠ½ΡΡ
ΡΠΎΠ»Ρ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ
ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΠ΄Π½ΠΈΠΌ ΠΈΠ· Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΠΏΡΠΈ ΠΊΠΎΡΠΎΡΡΡ
ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ
Π½Π°ΡΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ², ΡΠ²Π»ΡΠ΅ΡΡΡ Π-ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΉ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π»ΠΈΠΌΡΠ»Π΅ΠΉΠΊΠΎΠ·. Π¦Π΅Π»Ρ: ΠΎΡΠ΅Π½ΠΊΠ° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ²
ΠΊΠ°Π½Π½Π°Π±ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² Π½Π° Π-Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°Ρ
Π² ΡΡΠ΅ΠΏΠ°Π½ΠΎΠ±ΠΈΠΎΠΏΡΠ°ΡΠ°Ρ
Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
Π»Π΅ΠΉΠΊΠΎΠ·ΠΎΠΌ Π΄ΠΎ ΠΈ ΠΏΠΎΡΠ»Π΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π»Π΅ΡΠ΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ
ΠΏΡΡΠΈΠ½ΠΎΠ²ΡΡ
Π°Π½Π°Π»ΠΎΠ³ΠΎΠ². ΠΠ΅ΡΠΎΠ΄Ρ: Π±ΠΈΠΎΠΏΡΠ°ΡΡ ΠΏΠΎΠ»ΡΡΠΈΠ»ΠΈ ΡΡΡΠΈΠ½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ; ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π΄Π΅ΠΏΠ°ΡΠ°ΡΠΈΠ½ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΡΠ΅Π·ΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΠΎ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π΄ΡΡΠ΅. Π Π΅ΡΠ΅ΠΏΡΠΎΡΡ ΠΊΠ°Π½Π½Π°Π±ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ
ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π» Π°Π½ΡΠΈ-CB1 ΠΈ Π°Π½ΡΠΈ-CB2. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π½Π°Π»ΠΈΡΠΈΠ΅ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΎ ΠΏΡΠΈ ΠΏΠΎ-
ΠΌΠΎΡΠΈ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠ° CB1 Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π½ΠΈΠΆΠ΅, ΡΠ΅ΠΌ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ CB2 (17,0Β Β± 3,1% ΠΈ 92,1 1,7% ΡΠ²Π΅Π½Π½ΠΎ, p < 0,001). ΠΠΎΡΠ»Π΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ
Ρ 9 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΎΡΡ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ Π»Π΅ΠΉΠΊΠΎΠ·Π½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠ° (77,2 6,9% Π΄ΠΎ 30,2 6,5%, p = 0,007) ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ
ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠ° CB1 (24,4 4,8% Π΄ΠΎ 8,6 2,9%, p = 0,01), ΠΎΠ΄Π½Π°ΠΊΠΎ ΡΠ°Π·Π»ΠΈΡΠΈΠΉ Π² ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ CB2 Π½Π΅ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ (91,7
2,7% ΠΏΡΠΎΡΠΈΠ² 90,9 2,8%, p = 0,69). Π£ 4 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², Ρ ΠΊΠΎΡΠΎΡΡΡ
Π½Π΅ ΡΠ΄Π°Π»ΠΎΡΡ Π΄ΠΎΡΡΠΈΡΡ ΡΠ΅ΠΌΠΈΡΡΠΈΠΈ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π΄Π°ΠΆΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅
ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ². ΠΠΎ Π²ΡΠ΅Ρ
ΡΠ»ΡΡΠ°ΡΡ
ΠΌΠ°ΡΠΊΠΈΡΠΎΠ²ΠΊΠΈ Π°Π½ΡΠΈΡΠ΅Π»Π°ΠΌΠΈ ΠΊ ΠΎΠ±ΠΎΠΈΠΌ ΡΠΈΠΏΠ°ΠΌ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΠΊΠ°Π½Π½Π°Π±ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΠΏΠΎΠ»ΠΎ-
ΠΆΠΈΡΠ΅Π»ΡΠ½ΡΡ ΡΠ΅Π°ΠΊΡΠΈΡ. ΠΠΎΠ»Π΅Π΅ ΡΠΎΠ³ΠΎ, ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΠΊΠ°Π½Π½Π°Π±ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² Π½Π° Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°Π»ΠΈ ΠΏΡΠΈ
ΠΏΠΎΠΌΠΎΡΠΈ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ. ΠΡΠ²ΠΎΠ΄Ρ: Π² Ρ
ΠΎΠ΄Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ ΠΈΠ·Π½Π°ΡΠ°Π»ΡΠ½ΠΎΠ΅ Π½Π°Π»ΠΈΡΠΈΠ΅ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΠΊΠ°Π½Π½Π°Π±ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ²
Π½Π° Π-Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°Ρ
Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ Π»ΠΈΠΌΡΠΎΠ»Π΅ΠΉΠΊΠΎΠ·ΠΎΠΌ. Π£ΠΊΠ°Π·Π°Π½Π½ΡΠ΅ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΡ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ Π½ΠΎΠ²ΠΎΠΉ ΡΡΡΡΠΊΡΡΡΠΎΠΉ, ΠΊΠΎΡΠΎΡΠ°Ρ
ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π° Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π±ΠΎΠ»Π΅Π·Π½ΠΈ, ΠΈ ΠΌΠΎΠ³ΡΡ ΡΡΠΈΡΠ°ΡΡΡΡ Π½ΠΎΠ²ΠΎΠΉ ΠΌΠΈΡΠ΅Π½ΡΡ ΠΏΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
Π»Π΅ΠΉΠΊΠΎΠ·ΠΎΠΌ
Rapid and highly variable warming of lake surface waters around the globe
In this first worldwide synthesis of in situ and satellite-derived lake data, we find that lake summer surface water temperatures rose rapidly (global meanβ=β0.34Β°C decadeβ1) between 1985 and 2009. Our analyses show that surface water warming rates are dependent on combinations of climate and local characteristics, rather than just lake location, leading to the counterintuitive result that regional consistency in lake warming is the exception, rather than the rule. The most rapidly warming lakes are widely geographically distributed, and their warming is associated with interactions among different climatic factorsβfrom seasonally ice-covered lakes in areas where temperature and solar radiation are increasing while cloud cover is diminishing (0.72Β°C decadeβ1) to ice-free lakes experiencing increases in air temperature and solar radiation (0.53Β°C decadeβ1). The pervasive and rapid warming observed here signals the urgent need to incorporate climate impacts into vulnerability assessments and adaptation efforts for lakes.Peer reviewe
A global database of lake surface temperatures collected by in situ and satellite methods from 1985β2009
Peer reviewe
Rapid and highly variable warming of lake surface waters around the globe
Peer reviewed. Β©2015. The Authors.This is an open access article under theterms of the Creative CommonsAttribution-NonCommercial-N oDerivsLicense, which permits use and distri-bution in any medium, provided theoriginal work is properly cited, the use isnon-commerc ial and no modiο¬cationsor adaptations are made.In this first worldwide synthesis of in situ and satellite-derived lake data, we find that lake summer surface water temperatures rose rapidly (global mean = 0.34Β°C decade 1) between 1985 and 2009. Our analyses show that surface water warming rates are dependent on combinations of climate and
local characteristics, rather than just lake location, leading to the counterintuitive result that regional consistency in lake warming is the exception, rather than the rule. The most rapidly warming lakes are widely geographically distributed, and their warming is associated with interactions among different climatic factors βfrom seasonally ice-covered lakes in areas where temperature and solar radiation are increasing while cloud cover is diminishing (0.72Β°C decade 1) to ice-free lakes experiencing increases in air temperature
and solar radiation (0.53Β°C decade 1). The pervasive and rapid warming observed here signals the urgent need to incorporate climate impacts into vulnerability assessments and adaptation efforts for lakes
Rapid and highly variable warming of lake surface waters around the globe
peer reviewedIn this first worldwide synthesis of in situ and satellite-derived lake data, we find that lake summer surface water temperatures rose rapidly (global mean = 0.34Β°C decade-1) between 1985 and 2009. Our analyses show that surface water warming rates are dependent on combinations of climate and local characteristics, rather than just lake location, leading to the counterintuitive result that regional consistency in lake warming is the exception, rather than the rule. The most rapidly warming lakes are widely geographically distributed, and their warming is associated with interactions among different climatic factors - from seasonally ice-covered lakes in areas where temperature and solar radiation are increasing while cloud cover is diminishing (0.72Β°C decade-1) to ice-free lakes experiencing increases in air temperature and solar radiation (0.53Β°C decade-1). The pervasive and rapid warming observed here signals the urgent need to incorporate climate impacts into vulnerability assessments and adaptation efforts for lakes. Β© 2015. American Geophysical Union. All Rights Reserved
Global data set of long-term summertime vertical temperature profiles in 153 lakes
peer reviewedClimate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change. Β© 2021, The Author(s)
The unique methodological challenges of winter limnology
Winter is an important season for many limnological processes, which can range from biogeochemical transformations to ecological interactions. Interest in the structure and function of lake ecosystems under ice is on the rise. Although limnologists working at polar latitudes have a long history of winter work, the required knowledge to successfully sample under winter conditions is not widely available and relatively few limnologists receive formal training. In particular, the deployment and operation of equipment in below 0 C temperatures pose considerable logistical and methodological challenges, as do the safety risks of sampling during the ice covered period. Here, we consolidate information on winter lake sampling and describe effective methods to measure physical, chemical, and biological variables in and under ice. We describe variation in snow and ice conditions and discuss implications for sampling logistics and safety. We outline commonly encountered methodological challenges and make recommendations for best practices to maximize safety and efficiency when sampling through ice or deploying instruments in ice-covered lakes. Application of such practices over a broad range of ice-covered lakes will contribute to a better understanding of the factors that regulate lakes during winter and how winter conditions affect the subsequent ice-free period
Synchronous dynamics of zooplankton competitors prevail in temperate lake ecosystems
Although competing species are expected to exhibit compensatory dynamics (negative temporal covariation), empirical work has demonstrated that competitive communities often exhibit synchronous dynamics (positive temporal covariation). This has led to the suggestion that environmental forcing dominates species dynamics; however, synchronous and compensatory dynamics may appear at different length scales and/or at different times, making it challenging to identify their relative importance. We compiled 58 long-term datasets of zooplankton abundance in north-temperate and subtropical lakes and used wavelet analysis to quantify general patterns in the times and scales at which synchronous/compensatory dynamics dominated zooplankton communities in different regions and across the entire dataset. Synchronous dynamics were far more prevalent at all scales and times and were ubiquitous at the annual scale. Although we found compensatory dynamics in approximately 14% of all combinations of time period/scale/ lake, there were no consistent scales or time periods during which compensatory dynamics were apparent across different regions. Our results suggest that the processes driving compensatory dynamics may be local in their extent, while those generating synchronous dynamics operate at much larger scales. This highlights an important gap in our understanding of the interaction between environmental and biotic forces that structure communities. Β© 2014 The Author(s) Published by the Royal Society. All rights reserved