Stream VByte: Faster Byte-Oriented Integer Compression

Arrays of integers are often compressed in search engines. Though there are many ways to compress integers, we are interested in the popular byte-oriented integer compression techniques (e.g., VByte or Google's Varint-GB). They are appealing due to their simplicity and engineering convenience. Amazon's varint-G8IU is one of the fastest byte-oriented compression technique published so far. It makes judicious use of the powerful single-instruction-multiple-data (SIMD) instructions available in commodity processors. To surpass varint-G8IU, we present Stream VByte, a novel byte-oriented compression technique that separates the control stream from the encoded data. Like varint-G8IU, Stream VByte is well suited for SIMD instructions. We show that Stream VByte decoding can be up to twice as fast as varint-G8IU decoding over real data sets. In this sense, Stream VByte establishes new speed records for byte-oriented integer compression, at times exceeding the speed of the memcpy function. On a 3.4GHz Haswell processor, it decodes more than 4 billion differentially-coded integers per second from RAM to L1 cache

Information content of colored motifs in complex networks

We study complex networks in which the nodes of the network are tagged with different colors depending on the functionality of the nodes (colored graphs), using information theory applied to the distribution of motifs in such networks. We find that colored motifs can be viewed as the building blocks of the networks (much more so than the uncolored structural motifs can be) and that the relative frequency with which these motifs appear in the network can be used to define the information content of the network. This information is defined in such a way that a network with random coloration (but keeping the relative number of nodes with different colors the same) has zero color information content. Thus, colored motif information captures the exceptionality of coloring in the motifs that is maintained via selection. We study the motif information content of the C. elegans brain as well as the evolution of colored motif information in networks that reflect the interaction between instructions in genomes of digital life organisms. While we find that colored motif information appears to capture essential functionality in the C. elegans brain (where the color assignment of nodes is straightforward) it is not obvious whether the colored motif information content always increases during evolution, as would be expected from a measure that captures network complexity. For a single choice of color assignment of instructions in the digital life form Avida, we find rather that colored motif information content increases or decreases during evolution, depending on how the genomes are organized, and therefore could be an interesting tool to dissect genomic rearrangements.Comment: 21 pages, 8 figures, to appear in Artificial Lif

Mikroverkapselung von Hopfenextrakt zur Entwicklung eines biologischen Pflanzenschutzmittels gegen den Falschen Mehltau

The plant disease downy mildew threatens the organic production of numerous food plants, such as grapevine, hops and some fruits and vegetables. Today the only available fungicides in organic farming are based in copper. However, due to its accumulation in soils and negative effects on birds, small mammals and earthworms, the European Union demands a reduction of the heavy metal and even considers a ban, if an environmentally friendly alternative is found. Therefore, a new substance must be discovered or else the organic production of mentioned foods is in jeopardy. During the investigation of possible alternatives, an extract from hop cones was reported to have a fungicidal effect against downy mildew. However, to use the highly viscous, poorly water soluble and unstable extract as a plant protecting agent, its product properties need to be adapted to the application. The formulation method of microencapsulation was used in this research project in order to transform the hop extract into an applicable and highly active powder, which should provide a long-lasting effect. Using the spray congealing process, the active ingredients were encapsulated into a solid lipid matrix particle. Adjuvants, such as emulsifiers, dispersing agents and antioxidants, were included into the capsule to optimize the product features for its use in plant protection. Finally, leaf-disc-assays showed, that the developed capsule-prototypes can prevent an infection with the pathogen completely. Thereby it could be proven that microencapsulated hop extract is a promising plant protecting agent against downy mildew that might be able to replace copper in the future.Der Falsche Mehltau bedroht den ökologischen Anbau verschiedener Kulturen, wie Wein, Hopfen und einiger Obst- und Gemüsesorten. Zur Bekämpfung der Pflanzenkrankheit wurde bisher auf kupferhaltige Fungizide zurückgegriffen. Aufgrund der Anreicherung des Schwermetalls im Boden und seiner negativen Auswirkungen auf Vögel, Kleinsäuger und Regenwürmer fordert die Europäische Union eine Reduktion und zieht sogar ein Verbot in Betracht, sobald eine umweltfreundliche Alternative zur Verfügung steht. Damit weiterhin eine ökologische Produktion möglich bleibt, mussten neue wirksame Substanzen gefunden werden. Auf der Suche nach Alternativen konnte einem Extrakt aus den Dolden des Hopfens eine fungizide Wirkung gegen den Falschen Mehltau nachgewiesen werden. Damit der hochviskose, schlecht wasserlösliche und instabile Extrakt als Pflanzenschutzmittel eingesetzt werden kann, müssen die Produkteigenschaften an die Anwendung angepasst werden. Im Rahmen dieser Forschungsarbeit wurde die Mikroverkapselung als Formulierungsmethode gewählt, um den Hopfenextrakt in ein anwendbares und wirksames Pulver zu verwandeln, das einen möglichst lange anhaltenden Schutz bieten soll. Mithilfe der Sprüherstarrung wurden die Wirkstoffe in Lipid-Matrixkapseln eingebaut. Additive, wie Emulgatoren, Dispergiermittel und Antioxidantien, wurden verwendet, um die Kapseleigenschaften für die Anforderungen im Pflanzenschutz zu optimieren. Anhand von Blattscheibenversuchen konnte gezeigt werden, dass die entwickelten Kapselprototypen eine Infektion mit dem Schaderreger verhindern können. Die Untersuchungen lieferten den Beleg, dass mithilfe der Mikroverkapselung ein vielversprechendes biologisches Pflanzenschutzmittel aus dem Hopfenextrakt entwickelt wurde, welches in Zukunft Kupfer ersetzen könnte

112.social: Design and Evaluation of a Mobile Crisis App for Bidirectional Communication between Emergency Services and Citizens

Emergencies threaten human lives and overall societal continuity, whether or not the crises and disasters are induced by nature, such as earthquakes, floods and hurricanes, or by human beings, such as accidents, terror attacks and uprisings. In such situations, not only do citizens demand information about the damage and safe behaviour, but emergency services also require high quality information to improve situational awareness. For this purpose, there are currently two kinds of apps available: General-purpose apps, such as Facebook Safety Check or Twitter Alerts, already integrate safety features. Specific crisis apps, such as KATWARN in Germany or FEMA in the US, provide information on how to behave before, during and after emergencies, and capabilities for reporting incidents or receiving disaster warnings. In this paper, we analyse authorities’ and citizens’ information demands and features of crisis apps. Moreover, we present the concept, implementation and evaluation of a crisis app for incident reporting and bidirectional communication between authorities and citizens. Using the app, citizens may (1) report incidents by providing a category, description, location and multimedia files and (2) receive broadcasts and responses from authorities. Finally, we outline features, requirements and contextual factors for incident reporting and bidirectional communication via mobile app

Intracellular S. aureus in Osteoblasts in a Clinical Sample from a Patient with Chronic Osteomyelitis—A Case Report

The pathophysiological role of intracellular bacteria in osteomyelitis is still a matter of debate. Here, we demonstrate for the first time the presence of Staphylococcus aureus internalized into osteoblasts in human tissue samples of a case with a chronic osteomyelitis using ultrastructural transmission electron microscope analysis

The epidemiology and direct healthcare costs of aseptic nonunions in Germany – a descriptive report

Aims This observational cross-sectional study aimed to answer the following questions: 1) how has nonunion incidence developed from 2009 to 2019 in a nationwide cohort; 2) what is the age and sex distribution of nonunions for distinct anatomical nonunion localizations; and 3) how high were the costs for surgical nonunion treatment in a level 1 trauma centre in Germany? Methods Data consisting of annual International Classification of Diseases (ICD)-10 diagnosis codes from German medical institutions from 2009 to 2019, provided by the Federal Statistical Office of Germany (Destatis), were analyzed. Nonunion incidence was calculated for anatomical localization, sex, and age groups. Incidence rate ratios (IRRs) were determined and compared with a two-sample z-test. Diagnosis-related group (DRG)-reimbursement and length of hospital stay were retrospectively retrieved for each anatomical localization, considering 210 patients. Results In 2019, a total of 11,840 nonunion cases (17.4/100,000 inhabitants) were treated. In comparison to 2018, the incidence of nonunion increased by 3% (IRR 1.03, 95% confidence interval (CI) 0.53 to 1.99, p = 0.935). The incidence was higher for male cases (IRR female/male: 0.79, 95% CI 0.76 to 0.82, p = 0.484). Most nonunions occurred at the pelvic and hip region (3.6/100,000 inhabitants, 95% CI 3.5 to 3.8), followed by the ankle and foot as well as the hand (2.9/100,000 inhabitants each). Mean estimated DRG reimbursement for in-hospital treatment of nonunions was highest for nonunions at the pelvic and hip region (€8,319 (SD 2,410), p < 0.001). Conclusion Despite attempts to improve fracture treatment in recent years, nonunions remain a problem for orthopaedic and trauma surgery, with a stable incidence throughout the last decade

112.SOCIAL: Design and Evaluation of a Mobile Crisis App for Bidirectional Communication between Emergency Services and Citizen

Emergencies threaten human lives and overall societal continuity, whether or not the crises and disasters are induced by nature, such as earthquakes, floods and hurricanes, or by human beings, such as accidents, terror attacks and uprisings. In such situations, not only do citizens demand information about the damage and safe behaviour, but emergency services also require high quality information to improve situational awareness. For this purpose, there are currently two kinds of apps available: General-purpose apps, such as Facebook Safety Check or Twitter Alerts, already integrate safety features. Specific crisis apps, such as KATWARN in Germany or FEMA in the US, provide information on how to behave before, during and after emergencies, and capabilities for reporting incidents or receiving disaster warnings. In this paper, we analyse authorities’ and citizens’ information demands and features of crisis apps. Moreover, we present the concept, implementation and evaluation of a crisis app for incident reporting and bidirectional communication between authorities and citizens. Using the app, citizens may (1) report incidents by providing a category, description, location and multimedia files and (2) receive broadcasts and responses from authorities. Finally, we outline features, requirements and contextual factors for incident reporting and bidirectional communication via mobile app

Ultrastructural Evidence of Mitochondrial Dysfunction in Osteomyelitis Patients

Osteomyelitis is a difficult-to-treat disease with high chronification rates. First studies suggest increases in mitochondrial fission and mitochondrial dysfunction as possible contributors to the accumulation of intracellular reactive oxygen species and thereby to the cell death of infected bone cells. The aim of the present study is to analyze the ultrastructural impact of bacterial infection on osteocytic and osteoblastic mitochondria. Human infected bone tissue samples were visualized via light microscopy and transmission electron microscopy. Osteoblasts, osteocytes and their mitochondria were analyzed histomorphometrically and compared with the control group of noninfectious human bone tissue samples. The results depicted swollen hydropic mitochondria including depleted cristae and a decrease in matrix density in the infected samples. Furthermore, perinuclear clustering of mitochondria could also be observed regularly. Additionally, increases in relative mitochondrial area and number were found as a correlate for increased mitochondrial fission. In conclusion, mitochondrial morphology is altered during osteomyelitis in a comparable way to mitochondria from hypoxic tissues. This gives new perspectives on the treatment strategies since the manipulation of mitochondrial dynamics may improve bone cell survival as a potential new target for the therapy of osteomyelitis

Rifampicin restores extracellular organic matrix formation and mineralization of osteoblasts after intracellular Staphylococcus aureus infection

Aims Bone regeneration during treatment of staphylococcal bone infection is challenging due to the ability of Staphylococcus aureus to invade and persist within osteoblasts. Here, we sought to determine whether the metabolic and extracellular organic matrix formation and mineralization ability of S. aureus-infected human osteoblasts can be restored after rifampicin (RMP) therapy. Methods The human osteoblast-like Saos-2 cells infected with S. aureus EDCC 5055 strain and treated with 8 µg/ml RMP underwent osteogenic stimulation for up to 21 days. Test groups were Saos-2 cells + S. aureus and Saos-2 cells + S. aureus + 8 µg/ml RMP, and control groups were uninfected untreated Saos-2 cells and uninfected Saos-2 cells + 8 µg/ml RMP. Results The S. aureus-infected osteoblasts showed a significant number of intracellular bacteria colonies and an unusual higher metabolic activity (p < 0.005) compared to uninfected osteoblasts. Treatment with 8 µg/ml RMP significantly eradicated intracellular bacteria and the metabolic activity was comparable to uninfected groups. The RMP-treated infected osteoblasts revealed a significantly reduced amount of mineralized extracellular matrix (ECM) at seven days osteogenesis relative to uninfected untreated osteoblasts (p = 0.007). Prolonged osteogenesis and RMP treatment at 21 days significantly improved the ECM mineralization level. Ultrastructural images of the mineralized RMP-treated infected osteoblasts revealed viable osteoblasts and densely distributed calcium crystal deposits within the extracellular organic matrix. The expression levels of prominent bone formation genes were comparable to the RMP-treated uninfected osteoblasts. Conclusion Intracellular S. aureus infection impaired osteoblast metabolism and function. However, treatment with low dosage of RMP eradicated the intracellular S. aureus, enabling extracellular organic matrix formation and mineralization of osteoblasts at later stage

Microbiological and ultrastructural evaluation of bacteriophage 191219 against planktonic, intracellular and biofilm infection with Staphylococcus aureus

Infections of orthopaedic implants, such as fracture fixation devices and total-joint prostheses, are devastating complications. Staphylococcus aureus (S. aureus) is a predominant pathogen causing orthopaedic-implant biofilm infections that can also internalise and persist in osteoblasts, thus resisting antibiotic therapy. Bacteriophages are a promising alternative treatment approach. However, data on the activity of bacteriophages against S. aureus, especially during intracellular growth, and against in vivo biofilm formation on metals are scarce. Therefore, the present study evaluated the in vitro efficacy of S. aureus bacteriophage 191219, alone as well as in combination with gentamicin and rifampicin, to eradicate S. aureus strains in their planktonic stage, during biofilm formation and after internalisation into osteoblasts. Further, the invertebrate model organism Galleria mellonella was used to assess the activity of the bacteriophage against S. aureus biofilm on metal implants with and without antibiotics. Results demonstrated the in vitro efficacy of bacteriophage 191219 against planktonic S. aureus. The phage was also effective against in vitro S. aureus biofilm formation in a dose-dependent manner and against S. aureus internalised in an osteoblastic cell line. Transmission electron microscopy (TEM) analysis showed bacteriophages on S. aureus inside the osteoblasts, with the destruction of the intracellular bacteria and formation of new bacteriophages. For the Galleria mellonella infection model, single administration of phage 191219 failed to show an improvement in survival rate but appeared to show a not statistically significant enhanced effect with gentamicin or rifampicin. In summary, bacteriophages could be a potential adjuvant treatment strategy for patients with implant-associated biofilm infections