Protein Prenylation in the moss Physcomitrium patens

Protein prenylation is the addition of a 15 or 20 carbon lipid to a cysteine near carboxyl terminus of target proteins. Prenylation increases hydrophobicity, which facilitates membrane associations and protein-protein interaction. Protein prenylation is generally conserved among eukaryotes, and mutations in genes that carry out prenylation are lethal in animals and yeast. In plants prenylation mutations are not always lethal, but they do affect development, disease resistance, biofuel production, and drought response, among other processes of agricultural interest. To understand the evolutionary and developmental implications of plant protein prenylation, we used the sequenced, annotated, and translated genome of the moss Physcomitrium patens to search for proteins that meet minimal criteria for prenylation; specifically the presence of a sequence that includes cysteine and one of six specific amino acids at the C terminus. We then analyzed these proteins with an online prenylation prediction program to assess their likelihood of being prenylated based on additional sequence motifs. We then determined potential biological function of putative target proteins by using BLAST sequence similarity searches to identify related genes with known function. We plan to use these data to select prenylated proteins with functions of interest for in vivo studies using genetic and molecular tools to investigate their roles in plant development and environmental response. REFERENCES http://www.jbc.org/cgi/doi/10.1074/jbc.M115.673491https://ir.library.louisville.edu/uars/1051/thumbnail.jp

The Economic and Social Impacts to India and Its Citizens from Inward Foreign Direct Investment

Developing countries greatly need to boost economic investment in order to spur growth, boost jobs, transfer advanced technologies, reduce poverty and increase their capacity to expand social welfare programmes. Towards this end, most have constructed and coordinated vigorous policies to attract new foreign direct investment (FDI) and India is no exception to this (OECD, 2002; Stiglitz, 2006; Rao and Dhar, 2011b). Transnational corporations (TNCs), the source of most FDI, are powerful actors in the global economy and they, in turn, try to get the best possible deals from governments who are desperate to host them. In such a charged economic and political environment, there is no guarantee that FDI will implant these desired assets (Nunnenkamp, 2002; OECD, 2002; 2008; Lipsey, 2003). Indeed, there is evidence to suggest that TNCs bring a great many risks, as well as benefits, including labour exploitation, corrupt practices, including bribery, and the ability to unduly influence policy outcomes and monopolise domestic markets (Madeley, 1999; Richter, 2001; Farnsworth, 2004). Thus, TNCs carry great risks, for the citizens, economies and local communities of host countries enticing FDI in the hopes of securing seriously needed development assistance. This thesis analyses the potential benefits and disadvantages of FDI to India and its citizens as reported by elite policy stakeholders. The research is based on qualitative interviews in New Delhi, India with 40 participants from NGOs, IGOs, and policy and research organisations that target economic and social development issues. In addition, it utilises documentary and policy analysis methods in order to investigate India’s investment and development strategy through the Indian investment bureaux. Through this analysis, the thesis reveals that FDI to India brings both benefits and disadvantages to its citizens and economy. India’s current growth model is catering to the middle class consumer and employment needs and in this regard, FDI has increased opportunities and brought advantages. However, FDI is not bringing much benefit for those in the lower social classes. What’s worse, it is attributed to socioeconomic ills such as widening inequalities, increased social tensions, land displacement and the transport of low levels of global value chains which are rife with poor working conditions and exploitation

TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2

As multicellular organisms grow, spatial and temporal patterns of gene expression are strictly regulated to ensure that developmental programs are invoked at appropriate stages. In this work, we describe a putative transcriptional regulator in Arabidopsis, TACO LEAF (TCO), whose overexpression results in the ectopic activation of reproductive genes during vegetative growth. Isolated as an activation-tagged allele, tco-1D displays gene misexpression and phenotypic abnormalities, such as curled leaves and early flowering, characteristic of chromatin regulatory mutants. A role for TCO in this mode of transcriptional regulation is further supported by the subnuclear accumulation patterns of TCO protein and genetic interactions between tco-1D and chromatin modifier mutants. The endogenous expression pattern of TCO and gene misregulation in tco loss-of-function mutants indicate that this factor is involved in seed development. We also demonstrate that specific serine residues of TCO protein are targeted by the ubiquitous kinase CK2. Collectively, these results identify TCO as a novel regulator of gene expression whose activity is likely influenced by phosphorylation, as is the case with many chromatin regulators

Association mapping of resistance to tan spot in the Global Durum Panel

Tan spot, caused by the necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr), is an important disease of durum and common wheat worldwide. Compared to common wheat, less is known about the genetics and molecular basis of tan spot resistance in durum wheat. We evaluated 510 durum lines from the Global Durum wheat Panel (GDP) for sensitivity to the necrotrophic effectors (NEs) Ptr ToxA and Ptr ToxB, and for reaction to Ptr isolates representing races 1–5. Overall, susceptible durum lines were most prevalent in South Asia, the Middle East, and North Africa. Genome-wide association analysis showed the resistance locus Tsr7 was significantly associated with tan spot caused by races 2 and 3, but not races 1, 4, or 5. The NE sensitivity genes Tsc1 and Tsc2 were associated with susceptibility to Ptr ToxC- and Ptr ToxB-producing isolates, respectively, but Tsn1 was not associated with tan spot caused by Ptr ToxA-producing isolates, which further validates that the Tsn1-Ptr ToxA interaction does not play a significant role in tan spot development in durum. A unique locus on chromosome arm 2AS was associated with tan spot caused by race 4, a race once considered avirulent. A novel trait characterized by expanding chlorosis leading to increased disease severity caused by the Ptr ToxB-producing race 5 isolate DW5 was identified, and this trait was governed by a locus on chromosome 5B. We recommend that durum breeders select resistance alleles at the Tsr7, Tsc1, Tsc2, and the chromosome 2AS loci to obtain broad resistance to tan spot

The Planet in 2050

In 2050, the billions of people living on Earth have found a way to manage the planetary system effectively. Everyone has access to adequate food, shelter, and clean water. Human health is no longer considered outside of the health of the ecosystems in which people live. Ecological awareness is an integral part of education. People respond effectively to social and environmental hazards, and societies care for the most vulnerable amongst them. The economy, too, has shifted. Carbon dioxide management is under control, and energy efficiency is the norm. The remaining rainforests have been preserved. Coral reefs are recovering. Fish stocks are thriving. Is any of this really possible? How can our complex social and economic systems interact with a complex planetary system undergoing rapid change to create a future we all want? This book is a contextualised collation of ideas articulated by the 50 participants of the Planet 2050 workshop held in Lund in October 2008, as part of The Planet in 2050, an interdisciplinary Fast Track Initiative of the International Geosphere-Biosphere Programme. Participants were selected from academia and the sustainability practice community to give a wide-ranging, multi-cultural, trans-disciplinary set of perspectives. This collection explores four broad sectoral themes: energy and technologies; development, economies and culture; environment; and land use change. By doing so, this book emphasises the importance of a social dialogue on our collective future, and our responsibility to the Earth. It makes strong statements about what needs to happen to the global economy for a sustainable future and documents a new kind of scholarly discussion, engaging people from diverse knowledge communities in a spirit of exploration and reflexivity. The book provides a focus for dialogue and further study for postgraduates and researchers interested in global change as a multi-faceted, socio-environmental phenomenon, and as the book is written in an accessible scholarly style, assuming no prior specialist knowledge, it is also suitable for those involved in sustainability initiatives and polic

Widespread horse-based mobility arose around 2,200 BCE in Eurasia

Horses revolutionized human history with fast mobility. However, the timeline between their domestication and widespread integration as a means of transportation remains contentious. Here we assemble a large collection of 475 ancient horse genomes to assess the period when these animals were first reshaped by human agency in Eurasia. We find that reproductive control of the modern domestic lineage emerged ~2,200 BCE (Before Common Era), through close kin mating and shortened generation times. Reproductive control emerged following a severe domestication bottleneck starting no earlier than ~2,700 BCE, and coincided with a sudden expansion across Eurasia that ultimately resulted in the replacement of nearly every local horse lineage. This expansion marked the rise of widespread horse-based mobility in human history, which refutes the commonly-held narrative of large horse herds accompanying the massive migration of steppe peoples across Europe ~3,000 BCE and earlier. Finally, we detect significantly shortened generation times at Botai ~3,500 BCE, a settlement from Central Asia associated with corrals and a subsistence economy centered on horses. This supports local horse husbandry before the rise of modern domestic bloodlines.info:eu-repo/semantics/publishedVersio