Assessing DESS solution for the long-term preservation of nematodes from faecal samples

Preservation of biological samples is a relevant issue for many scientific disciplines. Although traditional preservers, such as formaldehyde or ethanol, imply major disadvantages related to health risks, DNA degradation and distortion of structures, they are widely used. Hence, the search for viable alternatives preserving morphometry and genetics seems necessary. Here we assess the suitability of DESS solution to preserve adult nematodes and their eggs in faeces. Concretely, faecal samples of terrestrial tortoises with oxyurids were used to: (i) compare the 1-month storage efficacy of eggs from different conservation protocols (faeces without preserver at -20 °C, faeces with DESS solution at room temperature, faeces with DESS solution at -20 °C and faeces with ethanol 70% at room temperature); (ii) address morphological nematode identification after 2 years of storage with DESS. We also corroborated that nematode DNA remained viable after 2 years. Overall, our results showed that DESS solution at room temperature is an advisable alternative to conserve both parasite eggs and adult nematodes for morphological identification and genetic purposes. It also offers the advantages of being low-cost, safe and suitable for fieldwork conditions and shipments without refrigeration for nematode preservation.MG was supported by a contract for postdoctoral researchers from the Generalitat Valenciana with reference APOSTD/2021/181. RCRC was supported by the European Union-Next Generation EU in the Maria Zambrano Program (ZAMBRANO 21-26). This research had the financial support of project PID2019-105682RA-I00, funded by the Spanish Ministry of Science and Innovation MCIN/AEI/10.13039/501100011033

Helmintos gastrointestinales en liebres (Lepus granatensis) del sureste de la Península Ibérica

The abdominal cavity and digestive organs of 85 Iberian hares (Lepus granatensis) from the Altiplano of the Region of Murcia (southeastern Spain) were studied. The overall prevalence of helminths was 87.7%, including nematodes (82.2% of prevalence), metacestodes (44.7%) and adult cestodes (5.9%). Three nematode species were found: Nematodiroides zembrae (78.8%), Passalurus ambiguus (9.4%) and Trichostrongylus retortaeformis (2.4%), while two cestode species were detected: Cittotaenia denticulata (5.9%) and Cysticercus pisiformis (44.7%). Our results show a positive relationship between N. zembrae intensity and the presence of C. pisiformis.La liebre ibérica (Lepus granatensis) es un mamífero ubicuo en la península ibérica con importantes implicaciones ecológicas y económicas. Para conocer el estatus sanitario de esta especie, se han estudiado la cavidad abdominal y órganos del aparato digestivo de 85 liebres ibéricas procedentes del Altiplano de la Región de Murcia (sureste de España). La prevalencia total de helmintos hallada fue del 87,7%, siendo la prevalencia de nematodos del 82,2%, la de metacestodos del 44,7% y, en el caso de las formas adultas de cestodos, del 5,9%. Las especies de nematodos encontradas fueron Nematodiroides zembrae (78.8%), Passalurus ambiguus (9,4%) y Trichostrongylus retortaeformis (2,4%), en tanto que las especies de cestodos detectadas fueron Cittotaenia denticulata (5,9%) y Cysticercus pisiformis (44,7%). Los resultados obtenidos demuestran la existencia de una relación positiva entre la intensidad de parasitación de N. zembrae y la presencia de C. pisiformis

Cardiopulmonary nematode infections in wild canids: Does the key lie on host-prey-parasite evolution?

©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2019.08.008Cardiopulmonary nematodes are among the most pathogenic parasites of domestic and wild canids. The aim of this study was to describe the species diversity, prevalence and infection intensity of these parasites in the Iberian wolf (Canis lupus signatus) and the red fox (Vulpes vulpes) in the northwest of the Iberian Peninsula. 257 foxes and 74 wolves were necropsied between 2008 and 2014. Four nematode species were identified: Angiostrongylus vasorum, Eucoleus aerophilus, Crenosoma vulpis and Filaroides hirthi. This last species was only found in wolves, being the first time that is cited worldwide in this wild canid. The overall parasite prevalence was significantly higher in foxes (70%) than in wolves (28%). Specifically, prevalences in foxes and wolves were, respectively, 43% and 22% for A. vasorum, 33% and 5% for E. aerophilus, and 30% and 9% for C. vulpis. The prevalence of F. hirthi was 16%. The A. vasorum intensity was significantly higher in foxes than in wolves. Differences between host species in the risk of infection would be associated to diverging feeding behavior, and possibly reflects a parasite-host adaptation related to host's hunting strategies and cardiorespiratory requirements. This study revealed an association between infection and environmental factors, and highlighted a wide variation in the spatial distribution of A. vasorum. Our results indicate that cardiopulmonary parasites are widespread in wild canids in northwest Spain, and further agrees with other studies indicating the expansion of A. vasorum in Europe and, therefore, the urgent need to investigate infection in dogs in sympatric areas

Studying the effects of multiple invasive mammals on Cory’s shearwater nest survival

The most common invasive mammals—mice, rats, and cats—have been introduced to islands around the world, where they continue to negatively affect native biodiversity. The eradication of those invasive mammals has had positive effects on many species of seabirds. However, the removal of one invasive mammal species may result in abundance changes of other species due to trophic and competitive interactions among species. Understanding the overall impact of several invasive species is a key challenge when evaluating the possible effects of eradication programmes. Here we assess the influence of the three most common invasive mammals on nest survival of Cory’s shearwater (Calonectris diomedea). We monitored six breeding colonies over 3 years and measured the activity of mice, rats and cats to examine the influence of invasive mammals on nest survival. We found that nest survival showed a similar temporal trend in all years, with lowest weekly survival probabilities shortly after chicks hatched. Cats were identified as major predators of chicks, but no measure of colony-specific cat activity was able to adequately explain variation in shearwater nest survival. Nest survival was on average 0.38 (95 % confidence interval 0.20–0.53) and varied among colonies as well as over time. We found a small positive influence of rats on nest survival, which may indicate that the presence of small rodents as alternative prey may reduce cat predation of chicks. Our findings suggest that the eradication of rodents alone may exacerbate the adverse effects of cats on shearwater nest survival.This work was included in the project LIFE07 NAT/P/000649 ‘Safe Islands for Seabirds’, coordinated by the Portuguese Society for the Study of Birds and co-financed by the European Commission.Peer Reviewe

The physiological cost of male-biased parasitism in a nearly monomorphic mammal

Background: Even though male-biased parasitism is common in mammals, little effort has been made to evaluate whether higher parasitic burden in males results in an extra biological cost, and thus a decrease in fitness. Body condition impairment and the augmentation of oxidative stress can be used as indicators of the cost of parasite infections. Here, we examined relationships between gastrointestinal and respiratory helminths, body condition and oxidative stress markers (glutathione peroxidase, paraoxonase-1) in 28 Pyrenean chamois (Rupicapra p. pyrenaica) sampled in autumn. Results: Only male chamois showed a reduction in body condition and higher oxidative stress due to parasite infection, likely because of the extremely high parasite burdens observed in males. Conclusions: This study made evident a disparity in the physiological cost of multiple parasitism between sexes in a wild mammal, mainly due to parasitic richness. Because of the similar life expectancy in male and female chamois, we suggest that males may have developed natural mechanisms to compensate for higher parasite loads during the rut

Questionnaire survey on Canine Leishmaniosis in southeastern Spain.

A veterinary practitioner's questionnaire survey on Canine Leishmaniosis (CanL) was carried out in southeast Spain to provide information on disease incidence, diagnosis and recommended preventive measures. A third of 101 responding veterinarians reported seeing more than 20 CanL cases during the previous year and clinical signs considered frequent or very frequent included weight loss, areas of exfoliative dermatitis, lymphadenomegaly, kidney dysfunction, onychogryphosis, cutaneous ulcers, anaemia, asthenia and apathy. Almost a third of veterinarians reported diagnosing CanL solely on the basis of lack of response to treatment for other chronic illnesses. The frequency of individual signs did not always reflect their diagnostic value. The majority of veterinarians, considered lymphadenomegaly, exfoliative dermatitis, onychogryphosis, weight loss and epistaxis to be the best disease indicators. However, up to 93% of veterinarians reported using laboratory tests to confirm infection, including indirect (biochemical and antibody assays) and direct (PCR and microscopy) methods. Among recommended measures to prevent CanL, insecticide impregnated collars and topical spot-on capsules were prescribed by 92% and 74% of respondents; however, at least 11% of veterinarians believed existing CanL preventive measures were ineffective. Multivariable statistical analysis indicated that seeing more than 20 CanL cases during the previous year was independently associated with being located in Alicante province, having a rural clientele and recognising that dogs with no other sign but a lack of response to other chronic disease treatments may be infected with CanL. There was a weak association between CanL incidence and rainfall in the months prior to typical sandfly peak abundance

What do we know about Angiostrongylus cantonensisin Spain? Current knowledge and future perspectives in a globalized world

© 2021 Wiley-VCH GmbH. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the published version of a published Work that appeared in final form in Transboundary and Emerging Diseases. To access the final edited and published work see https://doi.org/10.1111/tbed.14393Angiostrongylus cantonensis is an important emerging and zoonotic nematode that has been reported worldwide since its first description in Asia 86 years ago. Among places where this parasite has been recently described, insular regions of northwest Africa seem to be particularly relevant. We performed a systematic review in scientific databases to search and extract information about all reports describing cases of A. cantonensis in Spain until July 2021. As a result, seven surveys about the presence of this pathogen in Spanish insular regions were found, especially in the Canary Islands, as well as a non-autochthonous report of A. cantonensis in the mainland Spain. The lack of reliable information about the presence or absence of this nematode in the Iberian Peninsula highlights the need for further studies concerning this emerging pathogen since rodents acting as competent definitive hosts of A. cantonensis are widely distributed in the mainland. Moreover, the high maritime traffic daily registered in Spanish ports could be a main entrance pathway for A. cantonensis or its intermediate hosts in the country due to the frequent and direct connection between these points and regions where this pathogen is endemic. We encourage to focus further efforts on investigating the current epidemiological situation of A. cantonensis in the Iberian Peninsula, just as in other Mediterranean countries with similar epidemiological, ecological and geographical characteristics. In this sense, the relevant implications of this parasite for animal and public health make necessary to act integrating different viewpoints under the One Health perspectiv

Effect of exotic mammalian predators on parasites of Cory's shearwater: Ecological effect on population health and breeding success

Predator activity around the nests induces stress in breeding birds, which may have weaker immunity and are therefore more susceptible to parasite infections. The influence of predators on parasites has only been observed in land birds, and most studies are experimental. As seabird colonies offer excellent conditions for parasites, here we assess the influence of mouse, rat, and cat activity on parasites in Cory's shearwater (Calonectris diomedea borealis). Adults were examined for blood parasites and one adult and the juvenile from 53 nests were inspected for ectoparasites over two consecutive years (2010 and 2011). Nests differed in their physical characteristics and indices of mammal predator activity and were checked weekly to assess breeding success. Our results showed absence of blood parasites. Among the environmental factors, predator pressure received the most support (89 %) from the data as influencing nest ectoparasite intensity. Birds most infected had worse body condition, and breeding success was negatively influenced by predator activity and ectoparasite intensity. To our knowledge, this is the first analysis of the interaction between mammal predators and ectoparasites in seabird species and supports greater protection through eradication efforts. In addition, we provide the first data on the endoparasite fauna of Cory's shearwater.His study was made possible by LIFE07 NAT/P/ 000649 ‘Safe Islands for Seabirds’, coordinated by the Portuguese Society for the Study of Birds (SPEA) and co-financed by the European Union Commissio