RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair

Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility, as it participates in different DNA repair and damage tolerance pathways in which gap-filling DNA synthesis is required. In this work we show that human Polλ is conjugated with Small Ubiquitin-like MOdifier (SUMO) proteins both in vitro and in vivo, with Lys27 being the main target of this covalent modification. Polλ SUMOylation takes place in the nuclear pore complex and is mediated by the E3 ligase RanBP2. This post-translational modification promotes Polλ entry into the nucleus, which is required for its recruitment to DNA lesions and stimulated by DNA damage induction. Our work represents an advance in the knowledge of molecular pathways that regulate cellular localization of human Polλ, which are essential to be able to perform its functions during repair of nuclear DNA, and that might constitute an important point for the modulation of its activity in human cells

Ethics on the corporate websites of the main advertising agencies in Spain

Although a significant number of studies have been carried out in relation to the ethical criteria of advertising messages in Spain, little or no research has been done on the corporate ethics of advertising agencies. Based on a content analysis methodology, the research presented here provides a twofold account of the ethical dimension of agency self-advertising on the Internet by reading (1) corporate ethics statements and (2) corporate identity statements. The results of such reading disclose that only a minimal percentage of companies is bound by particular ethical commitments and only one advertising agency makes explicit reference to ethical concerns in its corporate identity statemen

Dynamics of river plumes in the South Brazilian Bight and South Brazil

Research articleThe plumes from the rivers of the South Brazilian Bight (SBB) and South Brazil (SB) were studied using a realistic model configuration. River plume variability on continental shelves is driven by the input of river runoff into the shelf, by wind variability, and also by ambient currents and its seasonal variability, especially the Brazil Current, which are realistically modelled in this study. It is presented a simulation of 4 years using a nested configuration, which allows resolving the region around Florianópolis with very high resolution (∼150 m). The dispersion of river plumes was assessed not only with the hydrodynamical model results but also by using passive tracers whose dynamics was analyzed seasonally. Several dyes were released together with the river discharges. This approach allowed calculating the depths of the riverine freshwater, and the resulting regions affected by the plumes. Northward intrusions of waters from the southern region, under the potential influence of the distant La Plata river plume, were evaluated with a Lagrangian approach. The local river plumes are confined to the inner shelf, except south of 30°S where discharges from Lagoa dos Patos disperse over the shelf in the spring and summer. The Brazil Current flowing southward over the slope prevents the river plumes from interaction with oceanic mesoscale dynamics. The river plumes are, thus, mainly controlled by the wind forcing. The plumes from SBB are able to disperse until SB following the southward wind regime typical of the summer. And both the SB and La Plata river plumes are also able to reach SBB, forced by the northward wind typical of the winter season, until the latitude of 25.5°S. A low salinity belt (below 35) is present along the coastal region of SB and SBB year-round, supported by contributions from both the large and small rivers. The interaction between the different plumes influences the dispersion patterns, shielding the Florianṕolis coastal region from plumes of distant rivers, and dispersing the plume of SBB rivers away from Santa Catarina Island as it disperses southward during the summer months.Versión del edito

Regulation of human Polλ by ATM-mediated phosphorylation during Non-Homologous End Joining

DNA double strand breaks (DSBs) trigger a variety of cellular signaling processes, collectively termed the DNA-damage response (DDR), that are primarily regulated by protein kinase ataxia-telangiectasia mutated (ATM). Among DDR activated processes, the repair of DSBs by non-homologous end joining (NHEJ) is essential. The proper coordination of NHEJ factors is mainly achieved through phosphorylation by an ATM-related kinase, the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), although the molecular basis for this regulation has yet to be fully elucidated. In this study we identify the major NHEJ DNA polymerase, DNA polymerase lambda (Polλ), as a target for both ATM and DNA-PKcs in human cells. We show that Polλ is efficiently phosphorylated by DNA-PKcs in vitro and predominantly by ATM after DSB induction with ionizing radiation (IR) in vivo. We identify threonine 204 (T204) as a main target for ATM/DNA-PKcs phosphorylation on human Polλ, and establish that its phosphorylation may facilitate the repair of a subset of IR-induced DSBs and the efficient Polλ-mediated gap-filling during NHEJ. Molecular evidence suggests that Polλ phosphorylation might favor Polλ interaction with the DNA-PK complex at DSBs. Altogether, our work provides the first demonstration of how Polλ is regulated by phosphorylation to connect with the NHEJ core machinery during DSB repair in human cells.España MINECO y la Comisión Europea (European Regional Development Fund) to J.F.R. RYC-2011-08752, BFU2013-44343-P) and to F.C-L. (SAF2014-55532-R)

Materiales microporoso germanatos, como catalizadores

Referencia OEPM: P9900953.-- Fecha de solicitud: 07/05/1999.-- Titular: Consejo Superior de Investigaciones Científicas (CSIC).Materiales microporoso germanatos, como catalizadores. Compuestos que cristalizan en el sistema ortorrómbico. Se han obtenido por síntesis hidrotermal en un reactor revestido de teflón por reacción de una mezcla de GeO2, Cu(NO3)2.H2O o Ag(NO3), amoniaco, agua y etilenglicol, en relación molar 1:0.1:2:2:0.1, con adición de propilamina y piridina 0.3:1, para el compuesto de Cu. Estos compuestos son catalizadores para la ciclopropanación de olefinas con etil diazoacetato.Peer reviewe

Editorial:Subcellular compartmentalization of plant antioxidants and ROS generating systems, volume II

JP, MR-R and FC were financed by ERDF-co-financed grants from the Junta de Andalucía (P18-FR-1359) and the Ministry of Science and Innovation (PID2019-103924GB-I00), Spain. CF was financed by BBSRC/GCRF Grant (BB/T008865/1), UK

Age-length keys availability for Atlantic bluefin tuna captured in the eastern management area

This paper analyzes the available direct ageing information in the last decade from Atlantic bluefin tuna caught in the eastern management area. To investigate differences among ALKs, a standard Von Bertalanffy growth function (VB) was fit to length at age data for each stratum. A deficient convergence of VB fitting to the asymptotic length due to the scarcity of old specimens was found for all available ALKs. After these analyses some records were identified as outliers (reading methodological issues) and removed from the data base.En prensa0,000

Sampling protocol for skeletal structures of North Atlantic albacore tuna (Thunnus alalunga) and ageing interpretation

This paper presents a standardized protocol for sampling skeletal hard parts (dorsal fin ray and otoliths), preparation and age interpretation of albacore first dorsal fin ray. Ageing of albacore is focused in interpretation and reading of annual temporal marks (translucent bands) in first ray of dorsal fin. Preparation of fin ray sections (spines) is presented in detail using two different methods. The spines are usually cut individually using a low speed cutter. Depending of the size of spines, a new procedure has been developed for small spines based on encasing spines in a matrix of plastic resin allowing multiple spines cutting. Interpretation of growth marks on spine sections is explained and examples are presented for a range of size of albacore aged using this method.Ce document présente un protocole standardisé pour l’échantillonnage des pièces dures du squelette (rayon de la nageoire dorsale et otolithes), la préparation et l’interprétation de l’âge du rayon du premier rayon de la nageoire dorsale du germon. La détermination de l’âge du germon se centre sur l’interprétation et la lecture des marques temporelles annuelles (bandes translucides) du premier rayon de la nageoire dorsale. La préparation des sections du rayon de la nageoire (épines) est présentée en détail à l’aide de deux méthodes différentes. Les épines sont généralement sectionnées individuellement en utilisant un couteau basse vitesse. Selon la taille des épines, une nouvelle procédure a été élaborée pour les petites épines, consistant à enfermer les épines dans une matrice en résine plastique qui permet le découpage de plusieurs épines. L’interprétation des marques de croissance sur les sections des épines est expliquée et des exemples sont donnés pour une gamme de tailles de germon dont l’âge a été déterminé à l’aide de cette méthode.En este documento se presenta un protocolo estandarizado para el muestreo de partes duras del esqueleto (otolitos y rayo de la aleta dorsal) y para la preparación e interpretación de la edad del primer rayo de la aleta dorsal del atún blanco. La determinación de la edad del atún blanco se centra en la interpretación y lectura de las marcas temporales anuales (bandas traslucidas) en el primer rayo de la aleta dorsal. Se presenta en detalle la preparación de las secciones del rayo de la aleta (espinas) utilizando dos métodos diferentes. Las espinas suelen cortarse generalmente de forma individual utilizando un cortador de baja velocidad. Dependiendo del tamaño de las espinas, se ha desarrollado un nuevo procedimiento para las espinas pequeñas, que consiste en introducir las espinas en una matriz de resina plástica que permite cortar espinas múltiples. Se explica la interpretación de las marcas de crecimiento en las secciones de espinas y se presentan ejemplos para una gama de tallas de atún blanco cuya edad se determinó utilizando este método