55 research outputs found
Aspects of plant cell growth and the actin cytoskeleton : lessons from root hairs
The main topic the thesis addresses is the role of the actin cytoskeleton in the growth process of plant cells. Plant growth implies a combination of cell division and cell expansion. The cytoskeleton, which exists of microtubules and actin filaments, plays a major role in both processes. Before cell growth takes place, a new cell is formed by cell division. The orientation of the division plane most often predicts the orientation of cell expansion, and a correct positioning of the division plane is therefore important for plant morphogenesis. During most stages of cell division microtubules and actin filaments have a similar configuration.In Chapter 1 (De Ruijter et al. , 1997, Acta Bot. Neerl . 46: 279-290) the cytoskeleton of microtubules has been visualized during all stages of cell division for long and short root tip cells of broad bean ( Vicia faba L.). In all cells the preprophase band of microtubules was positioned in the midplane of the cell, and perpendicular to the long axis of the root. It was observed that the spindle axis in short cells increasingly tilted, from meta- to anaphase, giving rise to oblique cell plates . It appears that this is caused by spatial constraints. During late-telophase, cell plates first rotated towards the transversal plane before they fused with the parental wall at the site of the earlier preprophase band. When cell division is completed, cells grow.Plant cell growth is the insertion of Golgi vesicles into the plasma membrane and the delivery of their content into the existing wall. If this wall is flexible and under turgor pressure, the membrane becomes larger and the wall expands. The basic principles of plant cell growth can best be studied in cells where this growth process takes place abundantly, that is in the tip of tip-growing cells of higher plants, such as root hairs and pollen tubes. In Chapter 2 (De Ruijter et al ., 1998, Plant J. 13: 341-350) characteristics for cell tip growth are being reported, studied by comparison of developmental stages of root hairs of vetch ( Vicia sativa L .), from their emergence to their maturity. It is further shown that lipochito-oligosaccharides (LCOs), well-characterized molecules that are excreted by bacteria, reinitiate cell tip growth in hairs that are terminating growth. Tip growth and the site of growth re-initiation correlates with the presence of a steep cytoplasmic calcium gradient at the plasma membrane. Furthermore, it was found that a spectrin-like protein is a good marker for tip growth, and co-localizes with the vesicle rich region, which is known to be present at the tip.Immunolocalization of this spectrin-like protein in plants was extended to a variety of growing cells and shows, in Chapter 3 (De Ruijter and Emons, 1993, Cell Biol. Int. 17: 169-182), that this protein is especially present in young growing cells. Molecular weight and iso-electric point determination, by means of immuno-blotting identified the plant spectrin-like protein. The anti-spectrin antibody also labels nuclei, which is further investigated in Chapter 4.To analyze the presence and localization of nuclear spectrin-like proteins, various plant tissues and isolated pea nuclei were labeled. The data presented in Chapter 4, show that the spectrin-like proteins are distributed in a speckled pattern and occasionally in tracks. The extraction procedures used indicate that the spectrin-like protein is part of the nuclear matrix in which it may be a stabilizing factor.In Chapter 5 (Miller, De Ruijter et al., 1999, Plant J. 17: 141-154) the actin cytoskeleton of vetch root hairs at their initiation and during their development is described. Actin filament bundles are the dynamic backbone of the cytoplasmic strands. Growing hairs show dense sub-apical fine bundles of actin filaments (FB-actin) and the very tip is devoid of actin filament bundles, whereas full-grown hairs have actin bundles looping through the tip. Similar actin configurations were obtained when root hairs were freeze substituted and immunolabeled with anti-actin, or chemically fixed by an improved method and stained with fluorescent phalloidin. Since LCOs had been shown to reinitiate root hair growth (Chapter 2), this signal molecule was used to study the actin cytoskeleton during growth reinitiation. Manipulation of the actin cytoskeleton with the actin filament capping drug cytochalasin D inhibited polar growth. However, root hair initiation and swelling after LCO application were not affected. We concluded that elongating FB-actin is another characteristic for tip growth.Indeed, LCOs altered the configuration of the actin cytoskeleton, which was studied in Chapter 6 (De Ruijter et al. , 1999, MPMI 12: 829-832). The density of sub-apical actin filament bundles increased within 3-15 minutes after the application of LCOs. By a quantitative approach we were able to define the minimal FB-actin density and minimal length, of the area with the FB-actin, needed for growth. Only in hairs in which FB-actin exceeded these values, tip growth was sustained or resumed. The rapid response of actin filaments indicates a role for the actin cytoskeleton in signal transduction cascades.Such a dynamic actin cytoskeleton must be regulated. Part of this regulation will be done by actin binding proteins. Therefore, our limited knowledge of actin binding proteins in plant cells is reviewed in Chapter 7 (De Ruijter and Emons, 1999, Plant Biology 1: 26-35) of the thesis.Chapter 8 summarizes the characteristics for growth in tip-growing cells and extrapolates them to cells that expand isodiametrically or predominantly along one length axis. We conclude that tip growing cells, like root hairs, shed light on basic principles of plant growth, and provide a system to monitor the effect of signal molecules on cell growth.</p
Rapportage analyse boomaantastingen
In zomer en najaar van 2008 zijn door de gemeente Alphen aan den Rijn meerdere vormen van boomaantasting geconstateerd. Het gaat daarbij om meerdere typen aantastingen op de stam van verschillende boomsoorten. Om meer duidelijkheid te krijgen omtrent de aard van de aantastingen is een microscopische analyse van de aantastingen uitgevoerd. Met een overzicht van boomsoorten waarvan de aard van de aantasting is onderzocht. In dit rapport is tevens een beeldverslag opgenome
Protein transfer to membranes upon shape deformation
Red blood cells, milk fat droplets, or liposomes all have interfaces consisting of lipid membranes. These particles show significant shape deformations as a result of flow. Here we show that these shape deformations can induce adsorption of proteins to the membrane. Red blood cell deformability is an important factor in several diseases involving obstructions of the microcirculatory system, and deformation induced protein adsorption will alter the rigidity of their membranes. Deformation induced protein transfer will also affect adsorption of cells onto implant surfaces, and the performance of liposome based controlled release systems. Quantitative models describing this phenomenon in biomaterials do not exist. Using a simple quantitative model, we provide new insight in this phenomenon. We present data that show convincingly that for cells or droplets with diameters upwards of a few micrometers, shape deformations induce adsorption of proteins at their interface even at moderate flow rate
Improvement of flexible rotor/active magnetic bearings system performance using pi-d control
Proportional–integral–derivative (PID) control is the most common control approach used to control active magnetic bearings system, especially in the case of supporting rigid rotors. In the case of flexible rotor support, the most common control is again PID control in combination with notch filters. Other control approaches, known as modern control theory, are still in development process and cannot be commonly found in real life industrial application. Right now, they are mostly used in research applications. In comparison to PID control, PI-D control implies that derivate element is in feedback loop instead in main branch of the system. In this paper, performances of flexible rotor/active magnetic bearing system were investigated in the case of PID and PI-D control, both in combination with notch filters. The performances of the system were analysed using an analysis in time domain by observing system response to step input and in frequency domain by observing a frequency response of sensitivity function
Different crystal morphologies lead to slightly different conformations of light-harvesting complex II as monitored by variations of the intrinsic fluorescence lifetime
In 2005, it was found that the fluorescence of crystals of the major light-harvesting complex LHCII of green plants is significantly quenched when compared to the fluorescence of isolated LHCII (A. A. Pascal et al., Nature, 2005, 436, 134-137). The Raman spectrum of crystallized LHCII was also found to be different from that of isolated LHCII but very similar to that of aggregated LHCII, which has often been considered a good model system for studying nonphotochemical quenching (NPQ), the major protection mechanism of plants against photodamage in high light. It was proposed that in the crystal LHCII adopts a similar (quenching) conformation as during NPQ and indeed similar changes in the Raman spectrum were observed during NPQ in vivo (A. V. Ruban et al., Nature, 2007, 450, 575-579). We now compared the fluorescence of various types of crystals, differing in morphology and age. Each type gave rise to its own characteristic mono-exponential fluorescence lifetime, which was 5 to 10 times shorter than that of isolated LHCII. This indicates that fluorescence is not quenched by random impurities and packing defects (as proposed recently by T. Barros et al., EMBO Journal, 2009, 28, 298-306), but that LHCII adopts a particular structure in each crystal type, that leads to fluorescence quenching. Most interestingly, the extent of quenching appears to depend on the crystal morphology, indicating that also the crystal structure depends on this crystal morphology but at the moment no data are available to correlate the crystals' structural changes to changes in fluorescence lifetime
Rapportage analyse boomaantastingen
In zomer en najaar van 2008 zijn door de gemeente Alphen aan den Rijn meerdere vormen van boomaantasting geconstateerd. Het gaat daarbij om meerdere typen aantastingen op de stam van verschillende boomsoorten. Om meer duidelijkheid te krijgen omtrent de aard van de aantastingen is een microscopische analyse van de aantastingen uitgevoerd. Met een overzicht van boomsoorten waarvan de aard van de aantasting is onderzocht. In dit rapport is tevens een beeldverslag opgenome
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