Hydrologic Observation, Model, and Theory Congruence on Evapotranspiration Variance: Diagnosis of Multiple Observations and Land Surface Models

This paper reconciles the state-of-the-art observations and simulations of evapotranspiration (ET) temporal variability through a diagnostic framework composed of an observation-model-theory triplet. Specifically, a confirmed theoretical tool, Evapotranspiration Temporal VARiance Decomposition (EVARD), is used as a benchmark to estimate ET monthly variance (σ2ET) across the contiguous United States (CONUS) with inputs including hydroclimatic observations, Gravity Recovery and Climate Experiment-based terrestrial water storage, four observation-based products (ETRSUW by the University of Washington, ETRSMOD16 from MOD16 Global Terrestrial ET Data Set, ETFLUXNET upscaled from of fluxtower observations, and ETGLEAM from Global Land Evaporation Amsterdam Model), and four operational land surface models (LSMs: MOSAIC, NOAH, NOAH-MP, and VIC). Five experiments are systematically designed to evaluate and diagnose possible errors and uncertainties in ET temporal variance estimated by the four observation-based ET products and the four LSM simulations. Based on the results of these experiments, the following diagnostic hypotheses regarding the uncertainty of the observation-based ET products are illustrated: ETRSUW captures the high σ2ET signals in the Midwest with negligible bias and moderate uncertainty over the contiguous United States; ETFLUXNET systematically underestimates σ2ET over CONUS but with the lowest level of uncertainty; ETRSMOD16 has medium bias with the highest level of uncertainty, and the spatial distribution of high σ2ET signal from ETRSMOD16 is different from other estimates; ETGLEAM has slight negative bias and medium uncertainty, and σ2ET in the West Coast is smaller than that from ETVARD. Regarding the LSMs, it is found that any of the four LSMs can be the best depending on a certain set of reference observations. The study reveals that LSMs have shown a reasonably worthy, though not perfect, capability in estimating ET and its variability in regions/aquifers with limited human interference. However, RS-based observations and theoretical estimates suggest that all the four LSMs examined in this study are not able to accurately predict the ET variability in regions/aquifers heavily influenced by human activities like Central Valley and High Plains aquifers; they all underestimate ET variability along the West Coast due to seasonal vegetation responses to Mediterranean climate and human water use. In addition, LSMs underestimate intraannual ET variance in California and the High Plains with underestimated terrestrial storage change components in ET variance, due to the inappropriate representation of groundwater pumping and its impact on ET and other hydrologic processes. This paper urges advancing hydrologic knowledge by finding congruence among models, data, and theories

Study on factors influencing social network service addiction among junior college students based on problem behavior theory

Objective·To construct a structural equation model based on problem behavior theory to conduct a study on social network addiction among junior college students.Methods·A cross-sectional questionnaire survey was conducted in a college in Shanghai. Logistic regression analysis was used to analyze the effects of gender, grade, study pressure, self-esteem, loneliness, depression, entrapment, defeat, interpersonal needs, perceived social support, smoking, alcohol, exercise, and academic achievement on social network service addiction. Based on the problem behavior theory, the structural equation model was used to construct a theoretical framework model of social network service addiction of junior college students.Results·60.31% of the total 980 participants had social network service addiction. The univariate Logistic regression results showed that depression, self-esteem, loneliness, frustration, drowsiness, social support, interpersonal needs, exercise, and academic performance had a significant impact on social network addiction. The structural equation model fitted well [S－Bχ2/df=8.03, goodness-of-fit index (GFI)=0.924, comparative fit index (CFI)=0.909, Tucker-Lewis index (TLI)=0.872, root mean square error of approximation (RMSEA)=0.096, standardized root mean square residual (SRMR)=0.070], suggesting the mutual influence between the personality system and the perceived environment system, between the personality system and the behavioral system, and between the perceived environment system and the behavior system interact (β=1.018, P=0.000; β=0.218, P=0.003; β=0.268, P=0.000). The influence of personality system and behavior system on social network service addiction was not statistically significant, while the perceived environment system had a significant positive impact on social network service addiction (β=0.481, P=0.001).Conclusion·Personality system and behavior system indirectly affect social network service addiction by influencing perceived environment system, and perceived environment system directly affects social network service addiction. For the problem of social network addiction among lower grade college students, it is necessary to fully respect the characteristics of college students, and work together from three levels of the system to reduce the risk of social network addiction among college students

A newly isolated roseophage represents a distinct member of Siphoviridae family.

BACKGROUND(#br)Members of the Roseobacter lineage are a major group of marine heterotrophic bacteria because of their wide distribution, versatile lifestyles and important biogeochemical roles. Bacteriophages, the most abundant biological entities in the ocean, play important roles in shaping their hosts’ population structures and mediating genetic exchange between hosts. However, our knowledge of roseophages (bacteriophages that infect Roseobacter) is far behind that of their host counterparts, partly reflecting the need to isolate and analyze the phages associated with this ecologically important bacterial clade.(#br)METHODS(#br)vB_DshS-R4C (R4C), a novel virulent roseophage that infects Dinoroseobacter shibae DFL12T, was isolated with the double-layer agar method. The phage morphology was visualized with transmission electron microscopy. We characterized R4C in-depth with a genomic analysis and investigated the distribution of the R4C genome in different environments with a metagenomic recruitment analysis.(#br)RESULTS(#br)The double-stranded DNA genome of R4C consists of 36,291 bp with a high GC content of 66.75%. It has 49 genes with low DNA and protein homologies to those of other known phages. Morphological and phylogenetic analyses suggested that R4C is a novel member of the family Siphoviridae and is most closely related to phages in the genus Cronusvirus. However, unlike the Cronusvirus phages, R4C encodes an integrase, implying its ability to establish a lysogenic life cycle. A terminal analysis shows that, like that of λ phage, the R4C genome utilize the ’cohesive ends’ DNA-packaging mechanism. Significantly, homologues of the R4C genes are more prevalent in coastal areas than in the open ocean.(#br)CONCLUSIONS(#br)Information about this newly discovered phage extends our understanding of bacteriophage diversity, evolution, and their roles in different environments

Directed Evolution of a Bright Near-Infrared Fluorescent Rhodopsin Using a Synthetic Chromophore

By engineering a microbial rhodopsin, Archaerhodopsin-3 (Arch), to bind a synthetic chromophore, merocyanine retinal, in place of the natural chromophore all-trans-retinal (ATR), we generated a protein with exceptionally bright and unprecedentedly red-shifted near-infrared (NIR) fluorescence. We show that chromophore substitution generates a fluorescent Arch complex with a 200-nm bathochromic excitation shift relative to ATR-bound wild-type Arch and an emission maximum at 772 nm. Directed evolution of this complex produced variants with pH-sensitive NIR fluorescence and molecular brightness 8.5-fold greater than the brightest ATR-bound Arch variant. The resulting proteins are well suited to bacterial imaging; expression and stability have not been optimized for mammalian cell imaging. By targeting both the protein and its chromophore, we overcome inherent challenges associated with engineering bright NIR fluorescence into Archaerhodopsin. This work demonstrates an efficient strategy for engineering non-natural, tailored properties into microbial opsins, properties relevant for imaging and interrogating biological systems

The Mediating Effect of Depression on the Relation Between Interpersonal Needs and Suicidal Ideation Among Chinese Transgender Women

Background: Transgender women are at high risk of depression and suicidal ideation. The interpersonal theory of suicide proposes that suicidal ideation could be a consequence of high interpersonal needs (thwarted belongingness and perceived burdensomeness). The current study tests this theory and investigates whether depression could mediate the relationship between interpersonal needs and suicidal ideation among transgender women in Shenyang, China. Methods: A total of 198 transgender women were recruited by snowball sampling. A cross-sectional study was conducted through a structured questionnaire. Suicidal ideation, depression, and interpersonal needs were assessed. Path analysis was used to carry out the research goals and the mediating effect of depression was tested. Results: There were nearly 37% of the participants reported lifetime suicidal ideation. Suicidal ideation was positively correlated with thwarted belongingness (t = −5.53, p \u3c 0.01) and perceived burdensomeness (t = −5.02, p \u3c 0.01). The direct effect from thwarted belongingness to suicidal ideation via depression was statistically significant (Std. β = 0.232, p \u3c 0.01). Depression could also mediate the indirect path from perceived burdensomeness to suicidal ideation through depression (Std. β = 0.222, p \u3c 0.01) although the direct path between them was not significant (Std. β = 0.046, p = 0.693). Conclusions: Depression fully mediated the relationship between perceived burdensomeness and suicidal ideation, and partially mediate the relationship between thwarted belongingness and suicidal ideation. To reduce the risk of suicidal ideation among transgender women, interventions targeting thwarted belongingness, perceived burdensomeness, and depression are needed

Gender-Specific and U-Shaped Relationship Between Serum Uric Acid and All-Cause Mortality Among Chinese Older Adults: A National Population-Based Longitudinal Study

Objectives: This study aimed to prospectively investigate gender-specific relationship between hyperuricemia and all-cause mortality among Chinese older adults.Methods: The study was based on the Chinese Longitudinal Healthy Longevity Survey (CLHLS) 2008–2018, a prospective nationwide cohort of older adults in China. Multivariate Cox proportional hazards models were used to estimate hazard ratios (HRs) and 95% CIs for all-cause mortality. Restricted cubic splines (RCS) were conducted to explore the dose-response relationship between SUA levels and all-cause mortality.Results: For older women, compared to the participants in the third quartile of SUA level, those in the highest quartile of SUA was associated with significantly higher risk of all-cause mortality in the fully adjusted model (HR: 1.41, 95% CI: 1.03–1.92). No significant associations between SUA levels and all-cause mortality were observed in older men. The present study further found a U-shaped non-linear relationship between SUA levels and all-cause mortality in both sexes of older population (P for non-linear <0.05).Conclusions: This study provided prospective epidemiological evidence for the predictive role of SUA on all-cause mortality among the Chinese aging population over 10 years of follow-up, while revealing considerable gender-related differences

The prevalence of childhood sexual experiences and intimate partner violence among transgender women in China: Risk factors for lifetime suicidal ideation

ObjectiveSeveral studies highlighted childhood sexual experiences (CSEs) and intimate partner violence (IPV) as risk factors that affected lifetime suicidal ideation. TW had higher rates of CSEs and IPV than cisgender people. The aim of this study was to comprehensively assess the prevalence of CSEs and IPV among TW and their association with lifetime suicidal ideation.MethodsA cross-sectional survey was conducted among 247 TW in Shenyang and Kunming, China, from April to September 2018. CSEs, IPV, and lifetime suicidal ideation were assessed. Logistic regression models were used to examine the association between self-reported CSEs under 18 years of age, IPV in adulthood, and lifetime suicidal ideation.ResultsIn the study, 14.2% (35/247) of the sample participants reported CSEs under 18 years of age; 44.9% (111/247) reported experiencing IPV in adulthood, including 18.6% (44/247) of physical IPV, 27.1% (67/247) of trans-specific identity IPV, 31.6% (78/247) of verbal IPV, and 19.4% (48/247) of sexual IPV; and 26.3% (65/247) had thought about attempting suicide at least one time. CSEs and any form of IPV were significantly associated with suicidal ideation in this sample population. A final stepwise multivariate logistic regression model found that both physical and verbal IPVs were significantly associated with suicidal ideation when controlling for other factors (ORm1 = 2.58, 95% confidence interval (CI) = 1.163–5.724; ORm2 = 2.72, 95% CI = 1.334–5.547).ConclusionsThe findings highlight the effects of CSEs and IPV among TW and suggest the need for research on suicide in the future. Suicide prevention efforts for this invisible and vulnerable population should focus on those with physical and verbal IPV

Alteration of Forkhead Box O (Foxo4) Acetylation Mediates Apoptosis of Podocytes in Diabetes Mellitus

The number of kidney podocytes is reduced in diabetic nephropathy. Advanced glycation end products (AGEs) accumulate in patients with diabetes and promote the apoptosis of podocyte by activating the forkhead box O4 (Foxo4) transcription factor to increase the expression of a pro-apoptosis gene, Bcl2l11. Using chromatin immunoprecipitation we demonstrate that AGE-modified bovine serum albumin (AGE-BSA) enhances Foxo4 binding to a forkhead binding element in the promoter of Bcl2lll. AGE-BSA also increases the acetylation of Foxo4. Lysine acetylation of Foxo4 is required for Foxo4 binding and transcription of Bcl2l11 in podocytes treated with AGE-BSA. The expression of a protein deacetylase that targets Foxo4 for deacetylation, sirtuin (Sirt1), is down regulated in cultured podocytes by AGE-BSA treatment and in glomeruli of diabetic patients. SIRT1 over expression in cultured murine podocytes prevents AGE-induced apoptosis. Glomeruli isolated from diabetic db/db mice have increased acetylation of Foxo4, suppressed expression of Sirt1, and increased expression of Bcl2l11 compared to non-diabetic littermates. Together, our data provide evidence that alteration of Foxo4 acetylation and down regulation of Sirt1 expression in diabetes promote podocyte apoptosis. Strategies to preserve Sirt1 expression or reduce Foxo4 acetylation could be used to prevent podocyte loss in diabetes