514 research outputs found
Shedding light on sporopollenin chemistry, with reference to UV reconstructions
Sporopollenin, which forms the outer wall of pollen and spores, contains a chemical signature of ultraviolet-B flux via concentrations of UV-B absorbing compounds (UACs), providing a proxy for reconstructing UV irradiance through time. Although Fourier transform infrared (FTIR) spectroscopy provides an efficient means of measuring UAC concentrations, nitrogen-containing compounds have the potential to bias the aromatic and hydroxyl bands used to quantify and standardise UAC abundances. Here, we explore the presence and possible influence of nitrogen in UV reconstruction via an FTIR study of Lycopodium spores from a natural shading gradient. We show that the UV-sensitive aromatic peak at 1510 cm− 1 is clearly distinguishable from the amide II peak at 1550 cm− 1, and the decrease in aromatic content with increased shading can be reconstructed using standardisation approaches that do not rely on the 3300 cm− 1 hydroxyl band. Isolation of the sporopollenin results in the loss of nitrogen-related peaks from the FTIR spectra, while the aromatic gradient remains. This confirms the lack of nitrogen in sporopollenin and its limited potential for impacting on palaeo-UV reconstructions. FTIR is therefore an appropriate tool for quantifying UACs in spores and pollen, and information on UV flux should be obtainable from fossil or processed samples
Acid-Labile Traceless Click Linker for Protein Transduction
Intracellular delivery of active proteins presents an interesting approach in research and therapy. We created a protein transduction shuttle based on a new traceless click linker that combines the advantages of click reactions with implementation of reversible pH-sensitive bonds. The azidomethyl-methylmaleic anhydride (AzMMMan) linker was found compatible with different click chemistries, demonstrated in bioreversible protein modification with dyes, polyethylene glycol, or a transduction carrier. Linkages were stable at physiological pH but reversible at the mild acidic pH of endosomes or lysosomes. We show that pH-reversible attachment of a defined endosome-destabilizing three-arm oligo(ethane amino)amide carrier generates an effective shuttle for protein delivery. The cargo protein nlsEGFP, when coupled via the traceless AzMMMan linker, experiences efficient cellular uptake and endosomal escape into the cytosol, followed by import into the nucleus. In contrast, irreversible linkage to the same shuttle hampers nuclear delivery of nlsEGFP which after uptake remains trapped in the cytosol. Successful intracellular delivery of bioactive ß-galactosidase as a model enzyme was also demonstrated using the pH-controlled shuttle system
Cross-verification of independent quantum devices
Quantum computers are on the brink of surpassing the capabilities of even the
most powerful classical computers. This naturally raises the question of how
one can trust the results of a quantum computer when they cannot be compared to
classical simulation. Here we present a verification technique that exploits
the principles of measurement-based quantum computation to link quantum
circuits of different input size, depth, and structure. Our approach enables
consistency checks of quantum computations within a device, as well as between
independent devices. We showcase our protocol by applying it to five
state-of-the-art quantum processors, based on four distinct physical
architectures: nuclear magnetic resonance, superconducting circuits, trapped
ions, and photonics, with up to 6 qubits and 200 distinct circuits
Genuine Counterfactual Communication with a Nanophotonic Processor
In standard communication information is carried by particles or waves.
Counterintuitively, in counterfactual communication particles and information
can travel in opposite directions. The quantum Zeno effect allows Bob to
transmit a message to Alice by encoding information in particles he never
interacts with. The first suggested protocol not only required thousands of
ideal optical components, but also resulted in a so-called "weak trace" of the
particles having travelled from Bob to Alice, calling the scalability and
counterfactuality of previous proposals and experiments into question. Here we
overcome these challenges, implementing a new protocol in a programmable
nanophotonic processor, based on reconfigurable silicon-on-insulator waveguides
that operate at telecom wavelengths. This, together with our telecom
single-photon source and highly-efficient superconducting nanowire
single-photon detectors, provides a versatile and stable platform for a
high-fidelity implementation of genuinely trace-free counterfactual
communication, allowing us to actively tune the number of steps in the Zeno
measurement, and achieve a bit error probability below 1%, with neither
post-selection nor a weak trace. Our demonstration shows how our programmable
nanophotonic processor could be applied to more complex counterfactual tasks
and quantum information protocols.Comment: 6 pages, 4 figure
Structural similarity analysis of midfacial fractures:a feasibility study
The structural similarity index metric is used to measure the similarity between two images. The aim here was to study the feasibility of this metric to measure the structural similarity and fracture characteristics of midfacial fractures in computed tomography (CT) datasets following radiation dose reduction, iterative reconstruction (IR) and deep learning reconstruction. Zygomaticomaxillary fractures were inflicted on four human cadaver specimen and scanned with standard and low dose CT protocols. Datasets were reconstructed using varying strengths of IR and the subsequently applying the PixelShine™ deep learning algorithm as post processing. Individual small and non-dislocated fractures were selected for the data analysis. After attenuating the osseous anatomy of interest, registration was performed to superimpose the datasets and subsequently to measure by structural image quality. Changes to the fracture characteristics were measured by comparing each fracture to the mirrored contralateral anatomy. Twelve fracture locations were included in the data analysis. The most structural image quality changes occurred with radiation dose reduction (0.980036±0.011904), whilst the effects of IR strength (0.995399±0.001059) and the deep learning algorithm (0.999996±0.000002) were small. Radiation dose reduction and IR strength tended to affect the fracture characteristics. Both the structural image quality and fracture characteristics were not affected by the use of the deep learning algorithm. In conclusion, evidence is provided for the feasibility of using the structural similarity index metric for the analysis of structural image quality and fracture characteristics
Iterative reconstruction and deep learning algorithms for enabling low-dose computed tomography in midfacial trauma
OBJECTIVES: The objective of this study was to quantitatively assess the image quality of Advanced Modeled Iterative Reconstruction (ADMIRE) and the PixelShine (PS) deep learning algorithm for the optimization of low-dose computed tomography protocols in midfacial trauma. STUDY DESIGN: Six fresh frozen human cadaver head specimens were scanned by computed tomography using both standard and low-dose scan protocols. Three iterative reconstruction strengths were applied to reconstruct bone and soft tissue data sets and these were subsequently applied to the PS algorithm. Signal-to-noise ratios (SNRs) and contrast-to-noise ratios (CNRs) were calculated for each data set by using the image noise measurements of 10 consecutive image slices from a standardized region of interest template. RESULTS: The low-dose scan protocol resulted in a 61.7% decrease in the radiation dose. Radiation dose reduction significantly reduced, and iterative reconstruction and the deep learning algorithm significantly improved, the CNR for bone and soft tissue data sets. The algorithms improved image quality after substantial dose reduction. The greatest improvement in SNRs and CNRs was found using the iterative reconstruction algorithm. CONCLUSION: Both the ADMIRE and PS algorithms significantly improved image quality after substantial radiation dose reduction
Strengthening impact assessment: a call for integration and focus
We suggest that the impact assessment community has lost its way based on our observation that impact assessment is under attack because of a perceived lack of efficiency. Specifically, we contend that the proliferation of different impact assessment types creates separate silos of expertise and feeds arguments for not only a lack of efficiency but also a lack of effectiveness of the process through excessive specialisation and a lack of interdisciplinary practice. We propose that the solution is a return to the basics of impact assessment with a call for increased integration around the goal of sustainable development and focus through better scoping. We rehearse and rebut counter arguments covering silo-based expertise, advocacy, democracy, sustainability understanding and communication. We call on the impact assessment community to rise to the challenge of increasing integration and focus, and to engage in the debate about the means of strengthening impact assessment
Generating a 4-photon Tetrahedron State: Towards Simultaneous Super-sensitivity to Non-commuting Rotations
It is often thought that the super-sensitivity of a quantum state to an
observable comes at the cost of a decreased sensitivity to other non-commuting
observables. For example, a squeezed state squeezed in position quadrature is
super-sensitive to position displacements, but very insensitive to momentum
displacements. This misconception was cleared with the introduction of the
compass state, a quantum state equally super-sensitive to displacements in
position and momentum. When looking at quantum states used to measure spin
rotations, N00N states are known to be more advantageous than classical methods
as long as they are aligned to the rotation axis. When considering the
estimation of a rotation with unknown direction and amplitude, a certain class
of states stands out with interesting properties. These states are equally
sensitive to rotations around any axis, are second-order unpolarized, and can
possess the rotational properties of platonic solids in particular dimensions.
Importantly, these states are optimal for simultaneously estimating the three
parameters describing a rotation. In the asymptotic limit, estimating all d
parameters describing a transformation simultaneously rather than sequentially
can lead to a reduction of the appropriately-weighted sum of the measured
parameters' variances by a factor of d. We report the experimental creation and
characterization of the lowest-dimensional such state, which we call the
"tetrahedron state" due to its tetrahedral symmetry. This tetrahedron state is
created in the symmetric subspace of four optical photons' polarization in a
single spatial and temporal mode, which behaves as a spin-2 particle. While
imperfections due to the hardware limit the performance of our method, we argue
that better technology can improve our method to the point of outperforming any
other existing strategy in per-photon comparisons.Comment: 13 pages, 6 figure
A clinical decision aid to discern patients without and with midfacial and mandibular fractures that require treatment (the REDUCTION-II study):a prospective multicentre cohort study
Purpose: To assess the diagnostic accuracy of physical examination findings and to construct clinical decision aids to discern emergency department patients without and with midfacial and mandibular fractures that require treatment. Methods: A prospective multicentre cohort study was conducted in four hospitals in the Netherlands. Consecutive maxillofacial trauma patients were included whereupon each patient underwent a standardized physical examination consisting of 15 and 14 findings for midfacial and mandibular trauma, respectively. The primary outcome was the decision whether to treat during the emergency department stay or within 24 h of admission. The diagnostic accuracy was calculated for the individual physical examination findings and ensuing clinical decision aids with the focus being on detecting midfacial and mandibular fractures that require active treatment. Results: A total of 766 midfacial trauma patients were identified of whom 339 (44.3%) had midfacial fractures. Of those, 74 (21.8%) required active treatment. A total of 280 mandibular trauma patients were identified of whom 66 (23.6%) had mandibular fractures. Of those, 37 (56.0%) required active treatment. The decision aid for midfacial trauma consisting of facial depression, epistaxis, ocular movement limitation, palpable step-off, objective malocclusion and tooth mobility or avulsion had a sensitivity of 97.3 (90.7–99.3), a specificity of 38.6 (35.0–42.3), and a negative predictive value of 99.3 (97.3–99.8). The decision aid for mandibular trauma consisting of mouth opening limitation, jaw movement pain, objective malocclusion and tooth mobility or avulsion resulted in a sensitivity of 100.0 (90.6–100.0), a specificity of 39.1 (33.2–45.4), and a negative predictive value of 100.0 (96.1–100.0). Conclusion: The clinical decision aids successfully identified midfacial and mandibular trauma patients requiring active fracture treatment and so may be useful in preventing unnecessary radiological procedures in the future. Trial Registration: The study was registered at ClinicalTrials.gov with the identifier NCT03314480
A clinical decision aid for patients with suspected midfacial and mandibular fractures (the REDUCTION-I study):A prospective multicentre cohort study
Purpose To assess physical examination findings related to maxillofacial trauma to identify patients at risk of midfacial and mandibular fractures and then to construct a clinical decision aid to rule out the presence of midfacial and mandibular fractures in emergency department patients. Methods We performed a prospective multicentre cohort study in four hospitals in the Netherlands, including consecutive patients with maxillofacial trauma. Each patient received a standardized physical examination consisting of 15 and 14 findings for midfacial and mandibular traumas, respectively. Consequently, clinical decision aids were constructed with the focus being on ruling out the presence of midfacial and mandibular fractures, and diagnostic accuracy was calculated. Results A total of 993 consecutive patients were identified of whom 766 and 280 patients were suspected of midfacial and mandibular fractures, respectively. Midfacial fractures were diagnosed in 339 patients (44.3%), whereas mandibular fractures were observed in 66 patients (23.6%). The decision aid for midfacial trauma consisting of peri-orbital hematoma, epistaxis, ocular movement limitation, infra-orbital nerve paresthesia, palpable step-off and tooth mobility or avulsion, produced a sensitivity of 89.7 (86.0-92.5), a specificity of 42.6 (38.0-47.4), and a negative predictive value of 83.9% (78.4-88.2). The decision aid for mandibular trauma consisting of the angular compression test, axial chin pressure test, objective malocclusion, tooth mobility or avulsion and the tongue blade bite test resulted in a sensitivity of 98.5 (91.9-99.7), a specificity of 34.6 (28.5-41.2), and a negative predictive value of 98.7% (92.8-99.8). Conclusion The constructed clinical decision aids for maxillofacial trauma may aid in stratifying patients suspected for midfacial and mandibular fractures to reduce unnecessary diagnostic imaging
- …