Promotions and Game Attendance: A Case Study of Collegiate Hockey in the South

Undergraduate Applie

Knowledge Summary 27: Death reviews: maternal, perinatal and child

Many maternal, perinatal and child deaths are preventable and progress towards Millennium Development Goals 4&5, to reduce child mortality and improve maternal health, has been insufficient in many parts of the world. Well-implemented death reviews provide opportunities to examine the circumstances surrounding a woman’s or child’s death, and improve the delivery of health services to prevent such deaths in the future. Several types of review processes exist to evaluate deaths in diverse settings, given different data availability and levels of service delivery. Both consistent surveillance and effective response are needed to ensure that maternal, perinatal and child deaths are identified and reviewed, so that recommendations can be made, and action can be taken to prevent further deaths

LG MS 079 Steven G. Bull Papers

Description: Papers include correspondence, photographs, publications, and ephemera documenting the beginning of the gay liberation movement in Maine and Steve Bull\u27s participation in the movement both in Maine and nationally, especially through his involvement with the founding of the Wilde-Stein Club at University of Maine Orono in 1973 and his chairmanship of the first Maine Gay Symposium in 1974. Letters received by Bull and his friends, both personally and as Wilde-Stein Club officials, are evidence of the attitudes of both supporters of gay liberation and its opponents in the 1970s. Bull\u27s research papers document the University of Maine\u27s reaction to the Wilde-Stein Club and national media coverage and opposition leading up to the first Maine Gay Symposium, and the University\u27s ultimate acceptance and support of the Club. Organizational records document Bull\u27s parents\u27 involvement in Parents of Gays, the Wilde-Stein Club\u27s early activities and members (including Karen Bye, John Frank, and Sturgis Haskins), and include photographs of the first Maine Gay Symposium. Publications include contemporaneous coverage of regional and national gay liberation, Marxism, and labor movements, and communication within the gay and lesbian communities. Date Range: 1974-2016 Size of Collection: 1 Linear Feet (2 Boxes); 1 Oversize Folder

Glyphosate Induces Neurotoxicity in Zebrafish

Glyphosate based herbicides (GBH) like Roundup® are used extensively in agriculture as well as in urban and rural settings as a broad spectrum herbicide. Its mechanism of action was thought to be specific only to plants and thus considered safe and non-toxic. However, mounting evidence suggests that GBHs may not be as safe as once thought as initial studies in frogs suggest that GBHs may be teratogenic. Here we utilize the zebrafish vertebrate model system to study early effects of glyphosate exposure using technical grade glyphosate and the Roundup® Classic formulation. We find morphological abnormalities including cephalic and eye reductions and a loss of delineated brain ventricles. Concomitant with structural changes in the developing brain, using in situ hybridization analysis, we detect decreases in genes expressed in the eye, fore and midbrain regions of the brain including pax2, pax6, otx2 and ephA4. However, we do not detect changes in hindbrain expression domains of ephA4 nor exclusive hindbrain markers krox-20 and hoxb1a. Additionally, using a Retinoic Acid (RA) mediated reporter transgenic, we detect no alterations in the RA expression domains in the hindbrain and spinal cord, but do detect a loss of expression in the retina. We conclude that glyphosate and the Roundup® formulation is developmentally toxic to the forebrain and midbrain but does not affect the hindbrain after 24 hour exposure

Enzyme activity and dynamics in near-anhydrous conditions

Water is widely assumed to be essential for life 1, although the exact molecular basis of this requirement is unclear 2-4. Water facilitates protein motions 5-9 and although enzyme activity has been demonstrated at low hydrations in organic solvents 10-13, such non-aqueous solvents may allow the necessary motions for catalysis. To examine enzyme function in the absence of solvation and bypass diffusional constraints we have tested the ability of an esterase to catalyse alcoholysis as an anhydrous powder, using a closed reaction system in which the substrates and products of the enzyme reaction are gaseous 14-15, and where the water content can be well defined 16. At hydrations equivalent to 3 (±2) molecules of water per molecule of enzyme, activity is observed that is several orders of magnitude greater than non-enzymatic catalysis. Neutron spectroscopy indicates that the fast (≤nanosecond) global anharmonic dynamics of the anhydrous functional enzyme are heavily suppressed. The results indicate that neither hydration water nor the solvent-activated fast anharmonic dynamics are required for enzyme function. An implication of these results is that one of the essential requirements of water for life may lie with its role as a diffusion medium rather than any of its more specific properties

Enzyme activity below the dynamical transition at 220 K

Enzyme activity requires the activation of anharmonic motions, such as jumps between potential energy wells. However, in general, the forms and time scales of the functionally important anharmonic dynamics coupled to motion along the reaction coordinate remain to be determined. In particular, the question arises whether the temperature-dependent dynamical transition from harmonic to anharmonic motion in proteins, which has been observed experimentally and using molecular dynamics simulation, involves the activation of motions required for enzyme function. Here we present parallel measurements of the activity and dynamics of a cryosolution of glutamate dehydrogenase as a function of temperature. The dynamical atomic fluctuations faster than ~100 ps were determined using neutron scattering. The results show that the enzyme remains active below the dynamical transition observed at ~220 K, i.e., at temperatures where no anharmonic motion is detected. Furthermore, the activity shows no significant deviation from Arrhenius behavior down to 190 K. The results indicate that the observed transition in the enzyme's dynamics is decoupled from the rate-limiting step along the reaction coordinate

Direct determination of vibrational density of states change on ligand binding to a protein

The change in the vibrational density of states of a protein (dihydrofolate reductase) on binding a ligand (methotrexate) is determined using inelastic neutron scattering. The vibrations of the complex soften significantly relative to the unbound protein. The resulting free-energy change, which is directly determined by the density of states change, is found to contribute significantly to the binding equilibrium

We All Go Home! The Evolution of Firefighter Accountability Systems

Accountability is arguably the single most important tool that a fire department has at its disposal. Although accountability systems have been used throughout history, one thing is proven: when there is no accountability system in place, it is only a matter of time before firefighters will get injured, or worse, lose their lives. This work will discuss the history of fire department methodology dealing with the creation of incident management accountability systems. In order to provide historical evidence that lack of an accountability system can cause injury or death, case studies of multiple fire departments that have reported line of duty deaths will be discussed. Next, the promising future of firefighter accountability systems will show that the previously discussed issues are actively being addressed. Finally, my personal experience within the fire service as well as accountability systems used in my department will be revealed