Ultraviolet Resonant Nanogap Antennas with Rhodium Nanocube Dimers for Enhancing Protein Intrinsic Autofluorescence

Plasmonic optical nanoantennas offer compelling solutions for enhancing light-matter interactions at the nanoscale. However, until now, their focus has been mainly limited to the visible and near-infrared regions, overlooking the immense potential of the ultraviolet (UV) range, where molecules exhibit their strongest absorption. Here, we present the realization of UV resonant nanogap antennas constructed from paired rhodium nanocubes. Rhodium emerges as a robust alternative to aluminum, offering enhanced stability in wet environments and ensuring reliable performance in the UV range. Our results showcase the nanoantenna ability to enhance the UV autofluorescence of label-free streptavidin and hemoglobin proteins. We achieve significant enhancements of the autofluorescence brightness per protein by up to 120-fold, and reach zeptoliter detection volumes enabling UV autofluorescence correlation spectroscopy (UV-FCS) at high concentrations of several tens of micromolar. We investigate the modulation of fluorescence photokinetic rates and report excellent agreement between experimental results and numerical simulations. This work expands the applicability of plasmonic nanoantennas into the deep UV range, unlocking the investigation of label-free proteins at physiological concentrations

Drug resistance and treatment failure in leishmaniasis: A 21st century challenge

Reevaluation of treatment guidelines for Old and New World leishmaniasis is urgently needed on a global basis because treatment failure is an increasing problem. Drug resistance is a fundamental determinant of treatment failure, although other factors also contribute to this phenomenon, including the global HIV/AIDS epidemic with its accompanying impact on the immune system. Pentavalent antimonials have been used successfully worldwide for the treatment of leishmaniasis since the first half of the 20th century, but the last 10 to 20 years have witnessed an increase in clinical resistance, e.g., in North Bihar in India. In this review, we discuss the meaning of “resistance” related to leishmaniasis and discuss its molecular epidemiology, particularly for Leishmania donovani that causes visceral leishmaniasis. We also discuss how resistance can affect drug combination therapies. Molecular mechanisms known to contribute to resistance to antimonials, amphotericin B, and miltefosine are also outlined

Comparison between the three porcine RN genotypes for growth, carcass composition and meat quality traits

A three-step experimental design has been carried out to add evidence about the existence of the RN gene, with two segregating alleles RN- and rn+, having major effects on meat quality in pigs, to estimate its effects on production traits and to map the RN locus. In the present article, the experimental population and sampling procedures are described and discussed, and effects of the three RN genotypes on growth and carcass traits are presented. The RN genotype had no major effect on growth performance and killing out percentage. Variables pertaining to carcass tissue composition showed that the RN- allele is associated with leaner carcasses (about 1 s.d. effect without dominance for back fat thickness, 0.5 s.d. effect with dominance for weights of joints). Muscle glycolytic potential (GP) was considerably higher in RN- carriers, with a maximum of a 6.85 s.d. effect for the live longissimus muscle GP. Physico-chemical characteristics of meat were also influenced by the RN genotype in a dominant way, ultimate pH differing by about 2 s.d. between homozygous genotypes and meat colour by about 1 s.d. Technological quality was also affected, with a 1 s.d. decrease in technological yield for RN- carriers. The RN genotype had a more limited effect on eating quality. On the whole, the identity between the acid meat condition and the RN- allele effect is clearly demonstrated (higher muscle GP, lower ultimate pH, paler meat and lower protein content), and the unfavourable relationship between GP and carcass lean to fat ratio is confirmed

Does turbulence affect the habitat choice of Atlantic salmon parr?

Habitat preferences of Atlantic salmon parr are commonly described using mean flow velocity, water depth, and substrate as habitat variables, and a variety of habitat models have been developed using these variables to predict habitat quality. However, Atlantic salmon parr live in highly turbulent streams and rivers, in which intense fluctuations of flow velocity occur. Habitat preferences that consider the high variability of flow velocity have not been studied, and this although it has been shown in laboratory experiments that turbulence may affect the behavior and energetics of fish. Consequently, we studied the use of turbulent flow by Atlantic salmon parr in Patapédia River, Québec, Canada using radio-telemetry. We analyzed summer habitat preferences of individual parr in relation to several dynamic hydraulic variables such as standard deviation of flow velocity, turbulent kinetic energy, Froude number, and shear stress, and compared them with the habitat availability within the river reach. Our results revealed that in a natural flow environment, parr display a high individual variability in habitat preferences in relation to flow turbulence. Such heterogeneous habitat preferences suggest that individuals are not constrained to single habitat types and exhibit flexible habitat use. Furthermore, no differences were observed in habitat preferences between the four daily periods (dawn, day, dusk, and night) within individual parr

Effects of experimental warming on Carbon sink function of a temperate pristine mire : The project PEATWARM

International audienceWithin the PEATWARM project, we use Sphagnum peatlands as a model to analyse their vulnerability to climate change using an experimental system (ITEX) that simulates in situ an increase in average temperature. We aim to determine the effects of temperature increase on the vegetation, the balance of above- and belowground gas fluxes (CO2 and CH4), the microbial diversity and activity in Sphagnum mosses and in peat, and the dynamics of labile and recalcitrant organic matter of peat. The ultimate objective is the creation of a biogeochemical model of C coupled with N and S cycles that includes interactions between these key compartments. Keywords: Global warming, C, N, S cycles, ITEX manipulations, ecosystem structure and function, biogeochemical model of C