The diffuse X-ray background spectrum from 3 to 50keV

The spectrum of the extragalactic diffuse X-ray background was measured with the GSFC cosmic X-ray experiment on HEAO-1 for regions of the sky away from known point sources and more than 20 deg from the galactic plane. A total exposure of 80 sq m-sec-sr is available at present. Free-free emission from an optically thin plasma of 40 plus or minus 5 keV provides an excellent description of the observed spectrum from 3 to 50 keV. This spectral shape is confirmed by measurements from 5 separate layers of three independent detectors. With an estimated absolute precision of about 10 percent, the intensity of the emission at 10 keV is 3.2 keV/keV-sq cm-sec-sr, a value consistent with the average of previously reported spectra. No other spectral features, such as iron line emission, are evident. This spectrum is not typical of known extragalactic objects. A uniform hot intergalactic medium of approximately 36 percent of the closure density of the universe would produce such a flux, although non-uniform models indicating less total matter are probably more realistic

X-ray observations of BL Lac objects

The BL Lac objects MK501 and MK421 were observed with OSO-8 and HEAO-1 X-ray detectors in the 2-60 keV band. Their spectra are similar with best fitting power laws having energy index -.4 alpha 4. A soft X-ray excess is indicated in their spectra. There was no detectable X-ray absorption with implied column densities N sub H 1.5 x 10 to the 22d power at sq cm. MK421 was a factor 6 weaker in November 1977 than in May 1977. An identification of PKS0548-322 with a new source H0548,-32 is suggested

Observations of the transient X-ray source 4U0115+63

Results of HEAO-2 pointed observations and Ariel 5 All Sky Monitor observations of the X-ray transient 4U0115+63 are presented. The transient source pulses with a period of 3.6136 s + or - .0004 s, has a hard spectrum typical of an X-ray binary pulsar, and has a broad iron line emission feature. A discussion of the transient behavior is given and inferences are made concerning the nature of the X-ray source based on the pointed data

Gene Discovery and Functional Genomics in the Pig

Advances in gene mapping and genomics in farm animals have been considerable over the past decade. Medium resolution linkage and physical maps have been reported, and specific chromosomal regions and genes associated with traits of biological and economic interest have been identified. We have reached an exciting stage in gene identification, mapping and quantitative trait locus discovery in pigs, as new molecular information is accumulating rapidly. Significant progress has been made by identifying candidate gene associations and low-resolution regions containing quantitative trail loci (QTL). However, we are still disadvantaged by the lack of tools available to efficiently use much of this new information. For example, current pig maps are neither of high enough resolution nor sufficiently informative at the comparative level for positional candidate gene cloning within QTL regions. As well, studying biological mechanisms underlying economically important traits such as reproduction is limited by the lack of molecular resources. This is especially important, as reproduction is very difficult to genetically improve by classical breeding methods due to the relatively low heritability and high expense in data collection. Thus, an improved understanding of porcine reproductive biology is of crucial economic importance, yet reproductive processes are poorly characterized at the molecular level. Recently, new methodologies have been brought to bear on a better understanding of pig molecular biology for accelerating genetic improvement in pigs. Several groups are developing molecular information in the pig, and the total Genbank sequence entries for porcine expressed genes have recently topped 100,000. Our Midwest EST Consortium has produced cDNA libraries containing the majority of genes expressed in major female reproductive tissues, and we have deposited nearly 15,000 gene sequences into public databases. These sequences represent over 8,900 different genes, based on sequence comparison among these data. Furthermore, we have developed computer software to automatically extract sequence similarity of these pig genes with their human counterparts, as well as the mapping information of these human homologues. Within our data set, we have identified nearly 1,500 pig genes with strong similarity to mapped human genes, and we are in the process of mapping 700 of these genes to improve the human-pig comparative map. This work and the complementary work of others can now be used to more rapidly understand and identify the genes controlling reproduction, so that genetic improvement of reproduction phenotypes can accelerate

Detection of selection signatures for ear carriage in Maltese goat breed

Selection and breeding practices in goats have led to the fixation of several traits. This is probably due to the standardization of several peculiar morphological characteristics that have always been one of the major exclusion criteria of individuals from selection. Among these, ear carriage is one of the most ancient and considered a signature of domestication in several species, such as the dog, pig, sheep and goat (Boyko et al., 2010). The availability of improved genomic analyses tools for goats may provide useful information on genes involved in this trait. By studying, for example, the homozygosity decay of haplotypes (contiguous length of alleles) such information can be detected. In the current study, we focused on the Maltese goat, a breed showing floppy ears, in comparison with other Italian breeds using a goat medium density SNP chip (Nicoloso et al., 2015). A total 48,767 SNP markers for 369 animals belonging to 16 breeds or populations were analyzed. Genotypes were imputed within population excluding markers without known position on the current genome assembly (ARS1, Bickhart et al., 2017). Population analysis using MDS, ADMIXTURE and fastSTRUCTURE confirmed the good differentiation among the populations. Integrated Haplotype Score (iHS, Sabeti et al., 2007) was performed for each population, comparing the regions detected on the Maltese breed with the others considered to detect genes that may be involved into shaping ear morphology. These results may provide new insights into ear carriage phenotype by detecting genes that play a pivotal role in shaping the goat phenotypic diversity

Mapping and Investigation of Two Novel Candidate Genes for Growth and Meat Quality traits in the Pig

Two new candidate genes for growth and meat quality traits were studied. The porcine beta-tropomyosin (TPM2) and the agouti-related protein (AGRP) genes were chosen based on their presumed role in growth and meat quality traits. These genes were genetically mapped and were linked to several markers on porcine chromosomes (SSC) 1 and 6, respectively. Both genes also were physically mapped using a pig/rodent somatic cell hybrid panel. The physical locations of the genes are consistent with linkage results and previous chromosome painting results indicating conserved (similar) regions between human and pig chromosomes. Association studies of the AGRP and TPM2 polymorphisms with growth and meat quality traits in commercial pig populations provided preliminary evidence that an AGRP polymorphism may be associated with variation in several traits of interest for pig breedin

Whole Genome Sequencing Provides Information on the Genomic Architecture and Diversity of Cultivated Gilthead Seabream (Sparus aurata) Broodstock Nuclei

The gilthead seabream (Sparus aurata) is a species of relevance for the Mediterranean aquaculture industry. Despite the advancement of genetic tools for the species, breeding programs still do not often include genomics. In this study, we designed a genomic strategy to identify signatures of selection and genomic regions of high differentiation among populations of farmed fish stocks. A comparative DNA pooling sequencing approach was applied to identify signatures of selection in gilthead seabream from the same hatchery and from different nuclei that had not been subjected to genetic selection. Identified genomic regions were further investigated to detect SNPs with predicted high impact. The analyses underlined major genomic differences in the proportion of fixed alleles among the investigated nuclei. Some of these differences highlighted genomic regions, including genes involved in general metabolism and development already detected in QTL for growth, size, skeletal deformity, and adaptation to variation of oxygen levels in other teleosts. The obtained results pointed out the need to control the genetic effect of breeding programs in this species to avoid the reduction of genetic variability within populations and the increase in inbreeding level that, in turn, might lead to an increased frequency of alleles with deleterious effects

EXIST: The Ultimate Spatial/Temporal Hard X-ray Survey

The Energetic X-ray Imaging Survey Telescope (EXIST) is a proposed mission to conduct an all-sky imaging hard x-ray (HX) survey (~5–600 keV) with ~0.05mCrab sensitivity (5σ; 6mo.; ~5–100keV) comparable to the ROSAT soft x-ray survey, and to provide the maximum sensitivity and resolution (spatial and temporal) HX imager as the Next Generation GRB mission. Its primary science goals are to i) identify and measure obscured AGN and constrain the accretion luminosity of the universe as well as the cosmic IR background from Blazar spectra coincident with GeV-TeV observations, ii) measure spectra, variability and locations for the faintest GRBs to study the most energetic events in the universe and the earliest epoch of star formation, and iii) study black holes on all scales, from x-ray transients to luminous AGN. EXIST would incorporate a very large area (~8m^2) imaging Cd-Zn-Te detector and coded aperture telescope array with nearly half-sky instantaneous view which images the full sky each orbit. With fixed zenith pointing, it could be mounted on the ISS or a free flyer and would complement both GLAST and Constellation-X science if launched before 2010, as recommended by the Astronomy and Astrophysics Decadal Survey

4U 1909+07: a Hidden Pearl

We present a detailed spectral and timing analysis of the High Mass X-ray Binary (HMXB) 4U 1909+07 with INTEGRAL and RXTE. 4U1909+07 is a persistent accreting X-ray pulsar with a period of approximately 605 s. The period changes erratically consistent with a random walk expected for a wind accreting system. INTEGRAL detects the source with an average of 2.4 cps (corresponding to 15mCrab), but sometimes exhibits flaring activity up to 50 cps (i.e. 300mCrab). The strongly energy dependent pulse profile shows a double peaked structure at low energies and only a single narrow peak at energies above 20 keV. The phase averaged spectrum is well described by a powerlaw modified at higher energies by an exponential cutoff and photoelectric absorption at low energies. In addition at 6.4 keV a strong iron fluorescence line and at lower energies a blackbody component are present. We performed phase resolved spectroscopy to study the pulse phase dependence of the spectral parameters: while most spectral parameters are constant within uncertainties, the blackbody normalization and the cutoff folding energy vary strongly with phase