2,025 research outputs found
ProfPPIdb: Pairs of physical protein-protein interactions predicted for entire proteomes
Motivation Protein-protein interactions (PPIs) play a key role in many cellular processes. Most annotations of PPIs mix experimental and computational data. The mix optimizes coverage, but obfuscates the annotation origin. Some resources excel at focusing on reliable experimental data. Here, we focused on new pairs of interacting proteins for several model organisms based solely on sequence-based prediction methods. Results We extracted reliable experimental data about which proteins interact (binary) for eight diverse model organisms from public databases, namely from Escherichia coli, Schizosaccharomyces pombe, Plasmodium falciparum, Drosophila melanogaster, Caenorhabditis elegans, Mus musculus, Rattus norvegicus, Arabidopsis thaliana, and for the previously used Homo sapiens and Saccharomyces cerevisiae. Those data were the base to develop a PPI prediction method for each model organism. The method used evolutionary information through a profile-kernel Support Vector Machine (SVM). With the resulting eight models, we predicted all possible protein pairs in each organism and made the top predictions available through a web application. Almost all of the PPIs made available were predicted between proteins that have not been observed in any interaction, in particular for less well-studied organisms. Thus, our work complements existing resources and is particularly helpful for designing experiments because of its uniqueness. Experimental annotations and computational predictions are strongly influenced by the fact that some proteins have many partners and others few. To optimize machine learning, recent methods explicitly ignored such a network-structure and rely either on domain knowledge or sequence-only methods. Our approach is independent of domain-knowledge and leverages evolutionary information. The database interface representing our results is accessible from https://rostlab.org/services/ppipair/. The data can also be downloaded from https://figshare.com/collections/ProfPPI-DB/4141784
Kinetic modelling and molecular dynamics simulation of ultracold neutral plasmas including ionic correlations
A kinetic approach for the evolution of ultracold neutral plasmas including
interionic correlations and the treatment of ionization/excitation and
recombination/deexcitation by rate equations is described in detail. To assess
the reliability of the approximations inherent in the kinetic model, we have
developed a hybrid molecular dynamics method. Comparison of the results reveals
that the kinetic model describes the atomic and ionic observables of the
ultracold plasma surprisingly well, confirming our earlier findings concerning
the role of ion-ion correlations [Phys. Rev. A {\bf 68}, 010703]. In addition,
the molecular dynamics approach allows one to study the relaxation of the ionic
plasma component towards thermodynamical equilibrium
Separation and identification of dominant mechanisms in double photoionization
Double photoionization by a single photon is often discussed in terms of two
contributing mechanisms, {\it knock-out} (two-step-one) and {\it shake-off}
with the latter being a pure quantum effect. It is shown that a quasi-classical
description of knock-out and a simple quantum calculation of shake-off provides
a clear separation of the mechanisms and facilitates their calculation
considerably. The relevance of each mechanism at different photon energies is
quantified for helium. Photoionization ratios, integral and singly differential
cross sections obtained by us are in excellent agreement with benchmark
experimental data and recent theoretical results.Comment: 4 pages, 5 figure
Ionization of clusters in strong X-ray laser pulses
The effect of intense X-ray laser interaction on argon clusters is studied
theoretically with a mixed quantum/classical approach. In comparison to a
single atom we find that ionization of the cluster is suppressed, which is in
striking contrast to the observed behavior of rare-gas clusters in intense
optical laser pulses. We have identified two effects responsible for this
phenomenon: A high space charge of the cluster in combination with a small
quiver amplitude and delocalization of electrons in the cluster. We elucidate
their impact for different field strengths and cluster sizes.Comment: 4 pages, 4 figure
Threshold detachment of negative ions by electron impact
The description of threshold fragmentation under long range repulsive forces
is presented. The dominant energy dependence near threshold is isolated by
decomposing the cross section into a product of a back ground part and a
barrier penetration probability resulting from the repulsive Coulomb
interaction. This tunneling probability contains the dominant energy variation
and it can be calculated analytically based on the same principles as Wannier's
description for threshold ionization under attractive forces. Good agreement is
found with the available experimental cross sections on detachment by electron
impact from , and .Comment: 4 pages, 4 figures (EPS), to appear in Phys.Rev.Lett, Feb. 22nd, 199
Dynamical stabilization of classical multi electron targets against autoionization
We demonstrate that a recently published quasiclassical M\oller type approach
[Geyer and Rost 2002, J. Phys. B 35 1479] can be used to overcome the problem
of autoionization, which arises in classical trajectory calculations for many
electron targets. In this method the target is stabilized dynamically by a
backward--forward propagation scheme. We illustrate this refocusing and present
total cross sections for single and double ionization of helium by electron
impact.Comment: LaTeX, 6 pages, 2 figures; submitted to J. Phys.
Universal exit probabilities in the TASEP
We study the joint exit probabilities of particles in the totally asymmetric
simple exclusion process (TASEP) from space-time sets of given form. We extend
previous results on the space-time correlation functions of the TASEP, which
correspond to exits from the sets bounded by straight vertical or horizontal
lines. In particular, our approach allows us to remove ordering of time moments
used in previous studies so that only a natural space-like ordering of particle
coordinates remains. We consider sequences of general staircase-like boundaries
going from the northeast to southwest in the space-time plane. The exit
probabilities from the given sets are derived in the form of Fredholm
determinant defined on the boundaries of the sets. In the scaling limit, the
staircase-like boundaries are treated as approximations of continuous
differentiable curves. The exit probabilities with respect to points of these
curves belonging to arbitrary space-like path are shown to converge to the
universal Airy process.Comment: 46 pages, 7 figure
Crossover to the KPZ equation
We characterize the crossover regime to the KPZ equation for a class of
one-dimensional weakly asymmetric exclusion processes. The crossover depends on
the strength asymmetry () and it occurs at
. We show that the density field is a solution of an
Ornstein-Uhlenbeck equation if , while for it is
an energy solution of the KPZ equation. The corresponding crossover for the
current of particles is readily obtained.Comment: Published by Annales Henri Poincare Volume 13, Number 4 (2012),
813-82
Meristematic connectome: A cellular coordinator of plant responses to environmental signals?
Mechanical stress in tree roots induces the production of reaction wood (RW) and the formation of new branch roots, both functioning to avoid anchorage failure and limb damage. The vascular cambium (VC) is the factor responsible for the onset of these responses as shown by their occurrence when all primary tissues and the root tips are removed. The data presented confirm that the VC is able to evaluate both the direction and magnitude of the mechanical forces experienced before coordinating the most fitting responses along the root axis whenever and wherever these are necessary. The coordination of these responses requires intense crosstalk between meristematic cells of the VC which may be very distant from the place where the mechanical stress is first detected. Signaling could be facilitated through plasmodesmata between meristematic cells. The mechanism of RW production also seems to be well conserved in the stem and this fact suggests that the VC could behave as a single structure spread along the plant body axis as a means to control the relationship between the plant and its environment. The observation that there are numerous morphological and functional similarities between different meristems and that some important regulatory mechanisms of meristem activity, such as homeostasis, are common to several meristems, supports the hypothesis that not only the VC but all apical, primary and secondary meristems present in the plant body behave as a single interconnected structure. We propose to name this structure \u201cmeristematic connectome\u201d given the possibility that the sequence of meristems from root apex to shoot apex could represent a pluricellular network that facilitates long-distance signaling in the plant body. The possibility that the \u201cmeristematic connectome\u201d could act as a single structure active in adjusting the plant body to its surrounding environment throughout the life of a plant is now proposed
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