Carriage of three plasmids in a single human clinical isolate of Clostridioides difficile

A subset of clinical isolates of Clostridioides difficile contains one or more plasmids and these plasmids can harbor virulence and antimicrobial resistance determinants. Despite their potential importance, C. difficile plasmids remain poorly characterized. Here, we provide the complete genome sequence of a human clinical isolate that carries three high-copy number plasmids from three different plasmid families that are therefore compatible. For two of these, we identify a region capable of sustaining plasmid replication in C. difficile that is also compatible with the plasmid pCD630 that is found in many laboratory strains. Together, our data advance our understanding of C. difficile plasmid biology.Molecular basis of bacterial pathogenesis, virulence factors and antibiotic resistanc

The SCUBA-2 Cosmology Legacy Survey: Ultraluminous star-forming galaxies in a z=1.6 cluster

We analyze new SCUBA-2 submillimeter and archival SPIRE far-infrared imaging of a z = 1.62 cluster, Cl 0218.3–0510, which lies in the UKIRT Infrared Deep Sky Survey/Ultra-Deep Survey field of the SCUBA-2 Cosmology Legacy Survey. Combining these tracers of obscured star-formation activity with the extensive photometric and spectroscopic information available for this field, we identify 31 far-infrared/submillimeter-detected probable cluster members with bolometric luminosities 1012 L ☉ and show that by virtue of their dust content and activity, these represent some of the reddest and brightest galaxies in this structure. We exploit ALMA submillimeter continuum observations, which cover one of these sources, to confirm the identification of a SCUBA-2-detected ultraluminous star-forming galaxy in this structure. Integrating the total star-formation activity in the central region of the structure, we estimate that it is an order of magnitude higher (in a mass-normalized sense) than clusters at z ~ 0.5-1. However, we also find that the most active cluster members do not reside in the densest regions of the structure, which instead host a population of passive and massive, red galaxies. We suggest that while the passive and active populations have comparable near-infrared luminosities at z = 1.6, MH ~ –23, the subsequent stronger fading of the more active galaxies means that they will evolve into passive systems at the present day that are less luminous than the descendants of those galaxies that were already passive at z ~ 1.6 (MH ~ –20.5 and MH ~ –21.5, respectively, at z ~ 0). We conclude that the massive galaxy population in the dense cores of present-day clusters were already in place at z = 1.6 and that in Cl 0218.3–0510 we are seeing continuing infall of less extreme, but still ultraluminous, star-forming galaxies onto a pre-existing structure

The ubiquitous catechol moiety elicits siderophore and angucycline production in Streptomyces

Microbial Biotechnolog

Shaping the risk for late-life neurodegenerative disease:A systematic review on prenatal risk factors for Alzheimer’s disease-related volumetric brain biomarkers

Environmental exposures including toxins and nutrition may hamper the developing brain in utero, limiting the brain's reserve capacity and increasing the risk for Alzheimer's disease (AD). The purpose of this systematic review is to summarize all currently available evidence for the association between prenatal exposures and AD-related volumetric brain biomarkers. We systematically searched MEDLINE and Embase for studies in humans reporting on associations between prenatal exposure(s) and AD-related volumetric brain biomarkers, including whole brain volume (WBV), hippocampal volume (HV) and/or temporal lobe volume (TLV) measured with structural magnetic resonance imaging (PROSPERO; CRD42020169317). Risk of bias was assessed using the Newcastle Ottawa Scale. We identified 79 eligible studies (search date: August 30th, 2020; Ntotal=24,784; median age 10.7 years) reporting on WBV (N = 38), HV (N = 63) and/or TLV (N = 5) in exposure categories alcohol (N = 30), smoking (N = 7), illicit drugs (N = 14), mental health problems (N = 7), diet (N = 8), disease, treatment and physiology (N = 10), infections (N = 6) and environmental exposures (N = 3). Overall risk of bias was low. Prenatal exposure to alcohol, opioids, cocaine, nutrient shortage, placental dysfunction and maternal anemia was associated with smaller brain volumes. We conclude that the prenatal environment is important in shaping the risk for late-life neurodegenerative disease

Early enteral tube feeding in optimizing treatment of hyperemesis gravidarum: The Maternal and Offspring outcomes after Treatment of HyperEmesis by Refeeding (MOTHER) randomized controlled trial

Background: Hyperemesis gravidarum (HG) leads to dehydration, poor nutritional intake, and weight loss. HG has been associated with adverse pregnancy outcomes such as low birth weight. Information about the potential effectiveness of treatments for HG is limited. Objective: We hypothesized that in women with HG, early enteral tube feeding in addition to standard care improves birth weight. Design: We performed a multicenter, open-label randomized controlled trial [Maternal and Offspring outcomes after Treatment of HyperEmesis by Refeeding (MOTHER)] in 19 hospitals in the Netherlands. A total of 116 women hospitalized for HG between 5 and 20 wk of gestation were randomly allocated to enteral tube feeding for ≥7 d in addition to standard care with intravenous rehydration and antiemetic treatment or to standard care alone. Women were encouraged to continue tube feeding at home. On the basis of our power calculation, a sample size of 120 women was anticipated. Analyses were performed according to the intention-to-treat principle. Results: Between October 2014 and March 2016 we randomly allocated 59 women to enteral tube feeding and 57 women to standard care. The mean ± SD birth weight was 3160 ± 770 g in the enteral tube feeding group compared with 3200 ± 680 g in the standard care group (mean difference: -40 g, 95% CI: -230, 310 g). Secondary outcomes, including maternal weight gain, duration of hospital stay, readmission rate, nausea and vomiting symptoms, decrease in quality of life, psychological distress, prematurity, and small-for-gestationalage, also were comparable. Of the women allocated to enteral tube feeding, 28 (47%) were treated according to protocol. Enteral tube feeding was discontinued within 7 d of placement in the remaining women, primarily because of its adverse effects (34%). Conclusions: In women with HG, early enteral tube feeding does not improve birth weight or secondary outcomes. Many women discontinued tube feeding because of discomfort, suggesting that it is poorly tolerated as an early routine treatment of HG

AzTEC half square degree survey of the SHADES fields - I. Maps, catalogues and source counts

We present the first results from the largest deep extragalactic mm-wavelength survey undertaken to date. These results are derived from maps covering over 0.7 deg2, made at λ= 1.1 mm, using the AzTEC continuum camera mounted on the James Clerk Maxwell Telescope. The maps were made in the two fields originally targeted at λ= 850 μm with the Submillimetre Common-User Bolometer Array (SCUBA) in the SCUBA Half-Degree Extragalactic Survey (SHADES) project, namely the Lockman Hole East (mapped to a depth of 0.9–1.3 mJy rms) and the Subaru/XMM–Newton Deep Field (mapped to a depth of 1.0–1.7 mJy rms). The wealth of existing and forthcoming deep multifrequency data in these two fields will allow the bright mm source population revealed by these new wide-area 1.1 mm images to be explored in detail in subsequent papers. Here, we present the maps themselves, a catalogue of 114 high-significance submillimetre galaxy detections, and a thorough statistical analysis leading to the most robust determination to date of the 1.1 mm source number counts. These new maps, covering an area nearly three times greater than the SCUBA SHADES maps, currently provide the largest sample of cosmological volumes of the high-redshift Universe in the mm or sub-mm. Through careful comparison, we find that both the Cosmic Evolution Survey (COSMOS) and the Great Observatories Origins Deep Survey (GOODS) North fields, also imaged with AzTEC, contain an excess of mm sources over the new 1.1 mm source-count baseline established here. In particular, our new AzTEC/SHADES results indicate that very luminous high-redshift dust enshrouded starbursts (S1.1mm > 3 mJy) are 25–50 per cent less common than would have been inferred from these smaller surveys, thus highlighting the potential roles of cosmic variance and clustering in such measurements. We compare number count predictions from recent models of the evolving mm/sub-mm source population to these sub-mm bright galaxy surveys, which provide important constraints for the ongoing refinement of semi-analytic and hydrodynamical models of galaxy formation, and find that all available models overpredict the number of bright submillimetre galaxies found in this survey