Sediment accumulation in the Broads. A report to the Broads Authority

Sediment accumulation rate data are described for 15 cores from 11 sites in the Norfolk and Suffolk Broads. Sediment dating was determined using a combination of radiometric ({210}^Pb, {137}^Cs) and spheroidal carbonaceous particle techniques. These approaches were combined to produce a ‘best available chronology’ for each site. However, at some sites results were still poor and the resulting data must be treated with caution

Acceptability, feasibility and challenges of implementing an HIV prevention intervention for people living with HIV/AIDS among healthcare providers in Mozambique: Results of a qualitative study

Despite the Mozambique government’s efforts to curb human immunodeficiency virus (HIV)/acquired immune deficiency syndrome (AIDS), national prevalence is 11.5% and support is needed to expand HIV-related services and improve program quality. Positive prevention (PP) programs, which prioritize HIV prevention with people living with HIV and AIDS (PLHIV), have been recognized as an important intervention for preventing new HIV infections. To address this, an evidence-based PP training intervention was implemented with HIV healthcare providers in Mozambique. This study focuses on the acceptability and feasibility of a PP intervention in HIV clinics from the healthcare provider perspective. In depth interviews were conducted with 31 healthcare providers from three provinces who participated in PP trainings in Mozambique. Interview data were coded using content analysis. Study data suggest that healthcare providers found PP acceptable, feasible to implement in their HIV work in clinic settings, and valued this strategy to improve HIV prevention. The PP training also led providers to feel more comfortable counseling their patients about prevention, with a more holistic approach that included HIV testing, treatment and encouraging PLHIV to live positively. While overall acceptance of the PP training was positive, several barriers to feasibility surfaced in the data. Patient-level barriers included resistance to disclosing HIV status due to fear of stigma and discrimination, difficulty negotiating for condom use, difficulty engaging men in testing and treatment, and the effects of poverty on accessing care. Providers also identified work environment barriers including high patient load, time constraints, and frequent staff turnover. Recognizing PP as an important intervention, healthcare providers should be trained to provide comprehensive prevention, care and treatment for PLHIV. Further work is needed to explore the complex social dynamics and cultural challenges such as gender inequalities, stigma and discrimination which hinder the full impact of PP interventions in this context.Keywords: positive prevention, feasibility and acceptability, healthcare provider, HIV/AID

Palaeoecological investigation of the past biological structure and function of the Trinity Broads

This is the final report to the Trinity Broads Partnership on the ‘Palaeoecological investigation of the past biological structure and function in the Trinity Broads’. The Trinity Broads are deeper and discrete from much of the rest of the Norfolk Broads and there has therefore been much speculation that their biology was different from the Broads in other river basins. The aim of the project was to employ palaeoecological techniques to investigate the past biological structure and function of the Broads, in particular the past aquatic flora

Consequences of fish kills for long-term trophic structure in shallow lakes: implications for theory and restoration

Fish kills are a common occurrence in shallow, eutrophic lakes, but their ecological consequences, especially in the long-term, are poorly understood. We studied the decadal-scale response of two UK shallow lakes to fish kills using a palaeolimnological approach. Eutrophic and turbid Barningham Lake experienced two fish kills in the early 1950s and late 1970s with fish recovering after both events, whereas less eutrophic, macrophyte-dominated Wolterton Lake experienced one kill event in the early 1970s from which fish failed to recover. Our palaeo-data show fish-driven trophic cascade effects across all trophic levels (covering benthic and pelagic species) in both lakes regardless of pre-kill macrophyte coverage and trophic status. In turbid Barningham Lake, similar to long-term studies of biomanipulations in other eutrophic lakes, effects at the macrophyte-level are shown to be temporary after the first kill (c.20 years) and non-existent after the second kill. In plant-dominated Wolterton Lake permanent fish disappearance failed to halt a long-term pattern of macrophyte community change (e.g. loss of charophytes and over-wintering macrophyte species) symptomatic of eutrophication. Important implications for theory and restoration ecology arise from our study. Firstly, our data support ideas of slow eutrophication-driven change in shallow lakes where perturbations are not necessary prerequisites for macrophyte loss. Secondly, the study emphasises a key need for lake managers to reduce external nutrient-loading if sustainable and long-term lake restoration is to be achieved. Our research highlights the enormous potential of multi-indicator palaeolimnology and alludes to an important need to consider potential fish kill signatures when interpreting results

Palaeoecological study of Rockland Broad

The decline in ecological quality and conservation value of European fresh waters is an all too common phenomenon. In lowland Britain the main anthropogenic impact on aquatic systems is that associated with elevated nutrient loading. The Broads are internationally important wetlands spanning a number of river basins in East Anglia. The Broads have suffered, along with other wetlands, and it is chiefly eutrophication that has had a deleterious effect on the system (Mason & Bryant 1975, Moss 1977) with a resultant decline in their ecological quality and conservation value. One of the changes in the ecological structure and functioning of shallow lakes in response to enrichment is an alteration in their macrophyte flora (Ris & Sand-Jensen 2001) and in extreme cases there may be the complete loss of submerged plants (Scheffer et al. 1993). The loss of the diversity of the macrophyte flora in the Broads is perhaps the main factor in the decline in their conservation value

Toxic metal enrichment and boating intensity: sediment records of antifoulant copper in shallow lakes of eastern England

Tributyltin (TBT), an aqueous biocide derived from antifouling paint pollution, is known to have impacted coastal marine ecosystems, and has been reported in the sediment of the Norfolk and Suffolk Broads, a network of rivers and shallow lakes in eastern England. In the marine environment, the 1987 TBT ban has resulted in expanded use of alternative biocides, raising the question of whether these products too have impacted the Broads ecosystem and freshwaters in general. Here we examine the lake sediment record in the Norfolk and Suffolk Broads for contamination by copper (Cu) (as an active biocide agent) and zinc (Zn) (as a component of booster biocides), to assess their occurrence and potential for causing environmental harm in freshwater ecosystems. We find that, after the introduction of leisure boating, there is a statistically significant difference in Cu enrichment between heavily and lightly boated sites, while no such difference exists prior to this time. At the heavily boated sites the onset of Cu enrichment coincides with a period of rapid increase in leisure boating. Such enrichment is maintained to the present day, with some evidence of continued increase. We conclude that Cu-based antifouling has measurably contaminated lakes exposed to boating, at concentrations high enough to cause ecological harm. Similar findings can be expected at other boated freshwater ecosystems elsewhere in the world

Using novel palaeolimnological techniques to define lake conservation objectives for three Cheshire meres

This is the final report to Natural England on the project „Using novel palaeolimnological techniques to define lake conservation objectives for three Cheshire meres‟: Melchett Mere, Tatton Mere and Comber Mere. The aim is to use existing and recently developed palaeoecological techniques to define reference conditions and assess the condition of selected Sites of Special Scientific Interest (SSSIs) in the Cheshire meres, and thereby assist in the setting of conservation objectives and management goals

Oncogenic GNAQ mutations are not correlated with disease-free survival in uveal melanoma

BackgroundRecently, oncogenic G protein alpha subunit q (GNAQ) mutations have been described in about 50% of uveal melanomas and in the blue nevi of the skin.MethodsGNAQ exon 5 was amplified from 75 ciliary body and choroidal melanoma DNAs and sequenced directly. GNAQ mutation status was correlated with disease-free survival (DFS), as well as other clinical and histopathological factors, and with chromosomal variations detected by FISH and CGH.ResultsOf the 75 tumour DNA samples analysed, 40 (53.3%) harboured oncogenic mutations in GNAQ codon 209. Univariate and multivariate analysis showed that GNAQ mutation status was not significantly correlated with DFS.ConclusionThe GNAQ mutation status is not suitable to predict DFS. However, the high frequency of GNAQ mutations may render it a promising target for therapeutic intervention