Dynamic Python-Based Method Provides Quantitative Analysis of Intercellular Junction Organization During S. pneumoniae Infection of the Respiratory Epithelium

Many respiratory pathogens compromise epithelial barrier function during lung infection by disrupting intercellular junctions, such as adherens junctions and tight junctions, that maintain intercellular integrity. This includes Streptococcus pneumoniae, a leading cause of pneumonia, which can successfully breach the epithelial barrier and cause severe infections such as septicemia and meningitis. Fluorescence microscopy analysis on intercellular junction protein manipulation by respiratory pathogens has yielded major advances in our understanding of their pathogenesis. Unfortunately, a lack of automated image analysis tools that can tolerate variability in sample-sample staining has limited the accuracy in evaluating intercellular junction organization quantitatively. We have created an open source, automated Python computer script called “Intercellular Junction Organization Quantification” or IJOQ that can handle a high degree of sample-sample staining variability and robustly measure intercellular junction integrity. In silico validation of IJOQ was successful in analyzing computer generated images containing varying degrees of simulated intercellular junction disruption. Accurate IJOQ analysis was further confirmed using images generated from in vitro and in vivo bacterial infection models. When compared in parallel to a previously published, semi-automated script used to measure intercellular junction organization, IJOQ demonstrated superior analysis for all in vitro and in vivo experiments described herein. These data indicate that IJOQ is an unbiased, easy-to-use tool for fluorescence microscopy analysis and will serve as a valuable, automated resource to rapidly quantify intercellular junction disruption under diverse experimental conditions

Repulsive Casimir force between silicon dioxide and superconductor

We have presented a detailed investigation of the Casimir interaction between the superconductor Bi2Sr2CaCu2O8+{\delta} (BSCCO) and silicon dioxide with the presence of bromobenzene in between. We found the dispersion force is repulsive and the magnitude of the force can be changed by varying the thickness of object and temperature. The repulsive force would provide a method to deal with the stiction problems and bring much meaningful from practical views.Comment: 3 pages, 2 figure

Stars quenching stars: how photoionization by local sources regulates gas cooling and galaxy formation

Current models of galaxy formation lack an efficient and physically constrained mechanism to regulate star formation (SF) in low and intermediate mass galaxies. We argue that the missing ingredient could be the effect of photoionization by local sources on the gas cooling. We show that the soft X-ray and EUV flux generated by SF is able to efficiently remove the main coolants (e.g., HeII, OV and FeIX) from the halo gas via direct photoionization. As a consequence, the cooling and accretion time of the gas surrounding star-forming galaxies may increase by one or two orders of magnitude. For a given halo mass and redshift, the effect is directly related to the value of the star formation rate (SFR). Our results suggest the existence of a critical SFR above which "cold" mode accretion is stopped, even for haloes with virial masses well below the critical shock-heating mass suggested by previous studies.The evolution of the critical SFR with redshift, for a given halo mass, resembles the respective steep evolution of the observed SFR for z<1. This suggests that photoionization by local sources would be able to regulate gas accretion and star formation, without the need for additional, strong feedback processes.Comment: 5 pages, 3 figures, accepted for publication in MNRAS Letter

Pore-forming activity of S. pneumoniae pneumolysin disrupts the paracellular localization of the epithelial adherens junction protein E-cadherin

Streptococcus pneumoniae, a common cause of community-acquired bacterial pneumonia, can cross the respiratory epithelial barrier to cause lethal septicemia and meningitis. S. pneumoniae pore-forming toxin pneumolysin (PLY) triggers robust neutrophil (PMN) infiltration that promotes bacterial transepithelial migration in vitro and disseminated disease in mice. Apical infection of polarized respiratory epithelial monolayers by S. pneumoniae at a multiplicity of infection (MOI) of 20 resulted in recruitment of PMNs, loss of 50% of the monolayer, and PMN-dependent bacterial translocation. Reducing the MOI to 2 decreased PMN recruitment two-fold and preserved the monolayer, but apical-to-basolateral translocation of S. pneumoniae remained relatively efficient. At both MOI of 2 and 20, PLY was required for maximal PMN recruitment and bacterial translocation. Co-infection by wild-type S. pneumoniae restored translocation by a PLY-deficient mutant, indicating that PLY can act in trans. Investigating the contribution of S. pneumoniae infection on apical junction complexes in the absence of PMN transmigration, we found that S. pneumoniae infection triggered the cleavage and mislocalization of the adherens junction (AJ) protein E-cadherin. This disruption was PLY-dependent at MOI of 2 and was recapitulated by purified PLY, requiring its pore-forming activity. In contrast, at MOI of 20, E-cadherin disruption was independent of PLY, indicating that S. pneumoniae encodes multiple means to disrupt epithelial integrity. This disruption was insufficient to promote bacterial translocation in the absence of PMNs. Thus, S. pneumoniae triggers cleavage and mislocalization of E-cadherin through PLY-dependent and -independent mechanisms, but maximal bacterial translocation across epithelial monolayers requires PLY-dependent neutrophil transmigration

Reliability of Pheromone Trap Catches and Maize Plant Damage as Criteria for Timing Fall Armyworm Control Interventions in Humid Forest Agroecology of Central Africa

Control of fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) since its invasion of Africa still depends on pesticides. Early detection of adults is considered the key to the success of larvae control in the crop field. However, FAW control thresholds based on current monitoring techniques are not well established in Africa. We investigated the efficacy of moth capture frequencies and FAW incidence levels as decision tools for FAW management. Experiments were conducted over two maize cropping seasons during which FAW incidence, severity, and larvae count were recorded during destructive sampling after the application of a homologated insecticide. During the first season, the FAW incidence ranged from 37.5 ± 5.6% in the 25% incidence threshold treatment to 48.1 ± 8.1% in the control. During the second season, the incidence was significantly lower in the 25% incidence threshold treatment (55.8 ± 5.7%) compared with the control (75.7 ± 3.0%). Over the two seasons, no significant difference in FAW damage severity was recorded between the treatments and control. The highest number of larvae per plant (4.0 ± 0.6) was observed in the 10% incidence threshold treatment. Insecticide application did not consistently contribute to reducing FAW incidence and observed plant damage did not translate into yield loss. FAW control needs further investigation to establish a threshold above which damage translates into yield loss, thus necessitating control intervention

The long-lived Type IIn SN 2015da: Infrared echoes and strong interaction within an extended massive shell star star star

In this paper we report the results of the first similar to four years of spectroscopic and photometric monitoring of the Type IIn supernova SN 2015da (also known as PSN J13522411+3941286, or iPTF16tu). The supernova exploded in the nearby spiral galaxy NGC 5337 in a relatively highly extinguished environment. The transient showed prominent narrow Balmer lines in emission at all times and a slow rise to maximum in all bands. In addition, early observations performed by amateur astronomers give a very well-constrained explosion epoch. The observables are consistent with continuous interaction between the supernova ejecta and a dense and extended H-rich circumstellar medium. The presence of such an extended and dense medium is difficult to reconcile with standard stellar evolution models, since the metallicity at the position of SN 2015da seems to be slightly subsolar. Interaction is likely the mechanism powering the light curve, as confirmed by the analysis of the pseudo bolometric light curve, which gives a total radiated energy greater than or similar to 10(51) erg. Modeling the light curve in the context of a supernova shock breakout through a dense circumstellar medium allowed us to infer the mass of the prexisting gas to be similar or equal to 8 M-circle dot, with an extreme mass-loss rate for the progenitor star similar or equal to 0.6 M-circle dot yr(-1), suggesting that most of the circumstellar gas was produced during multiple eruptive events. Near- and mid-infrared observations reveal a fluxexcess in these domains, similar to those observed in SN 2010jl and other interacting transients, likely due to preexisting radiatively heated dust surrounding the supernova. By modeling the infrared excess, we infer a mass greater than or similar to 0.4 x 10(-3) M-circle dot for the dustSpanish MICINN gran

Observations of Type Ia Supernova 2014J for Nearly 900 Days and Constraints on Its Progenitor System

We present extensive ground-based and $Hubble~Space~Telescope$ ($HST$) photometry of the highly reddened, very nearby type Ia supernova (SN Ia) 2014J in M82, covering the phases from 9 days before to about 900 days after the $B$-band maximum. SN 2014J is similar to other normal SNe Ia near the maximum light, but it shows flux excess in the $B$ band in the early nebular phase. This excess flux emission can be due to light scattering by some structures of circumstellar materials located at a few 10$^{17}$ cm, consistent with a single degenerate progenitor system or a double degenerate progenitor system with mass outflows in the final evolution or magnetically driven winds around the binary system. At t$\sim$+300 to $\sim$+500 days past the $B$-band maximum, the light curve of SN 2014J shows a faster decline relative to the $^{56}$Ni decay. Such a feature can be attributed to the significant weakening of the emission features around [Fe III] $\lambda$4700 and [Fe II] $\lambda$5200 rather than the positron escape as previously suggested. Analysis of the $HST$ images taken at t$>$600 days confirms that the luminosity of SN 2014J maintains a flat evolution at the very late phase. Fitting the late-time pseudo-bolometric light curve with radioactive decay of $^{56}$Ni, $^{57}$Ni and $^{55}$Fe isotopes, we obtain the mass ratio $^{57}$Ni/$^{56}$Ni as $0.035 \pm 0.011$, which is consistent with the corresponding value predicted from the 2D and 3D delayed-detonation models. Combined with early-time analysis, we propose that delayed-detonation through single degenerate scenario is most likely favored for SN 2014J.Comment: 28 pages, 12 figures. Accepted for publication in Ap

Environmental drivers of distribution and reef development of the Mediterranean coral Cladocora caespitosa

Cladocora caespitosa is the only Mediterranean scleractinian similar to tropical reef-building corals. While this species is part of the recent fossil history of the Mediterranean Sea, it is currently considered endangered due to its decline during the last decades. Environmental factors affecting the distribution and persistence of extensive bank reefs of this endemic species across its whole geographic range are poorly understood. In this study, we examined the environmental response of C. caespitosa and its main types of assemblages using ecological niche modeling and ordination analysis. We also predicted other suitable areas for the occurrence of the species and assessed the conservation effectiveness of Mediterranean marine protected areas (MPAs) for this coral. We found that phosphate concentration and wave height were factors affecting both the occurrence of this versatile species and the distribution of its extensive bioconstructions in the Mediterranean Sea. A set of factors (diffuse attenuation coefficient, calcite and nitrate concentrations, mean wave height, sea surface temperature, and shape of the coast) likely act as environmental barriers preventing the species from expansion to the Atlantic Ocean and the Black Sea. Uncertainties in our large-scale statistical results and departures from previous physiological and ecological studies are also discussed under an integrative perspective. This study reveals that Mediterranean MPAs encompass eight of the ten banks and 16 of the 21 beds of C. caespitosa. Preservation of water clarity by avoiding phosphate discharges may improve the protection of this emblematic species.Spanish Ministry of Economy and Competitiveness [CTM2014-57949-R]info:eu-repo/semantics/publishedVersio

The optical properties of three type II supernovae: 2014cx, 2014cy and 2015cz

We present the photometric and spectroscopic analysis of three Type II SNe: 2014cx, 2014cy and 2015cz. SN 2014cx is a conventional Type IIP with a shallow slope (0.2 mag/50d) and an atypical short plateau ($\sim$86 d). SNe 2014cy and 2015cz show relatively large decline rates (0.88 and 1.64 mag/50d, respectively) at early times before settling to the plateau phase, unlike the canonical Type IIP/L SN light curves. All of them are normal luminosity SN II with an absolute magnitude at mid-plateau of M$_{V,14cx}^{50}$=$-$16.6$\pm$0.4$\,\rm{mag}$, M$_{V,14cy}^{50}$=$-$16.5$\,\pm\,$0.2$\,\rm{mag}$ and M$_{V,15cz}^{50}$=$-$17.4$\,\pm\,$0.3$\,\rm{mag}$. A relatively broad range of $^{56}$Ni masses is ejected in these explosions (0.027-0.070 M$_\odot$). The spectra show the classical evolution of Type II SNe, dominated by a blue continuum with broad H lines at early phases and narrower metal lines with P Cygni profiles during the plateau. High-velocity H I features are identified in the plateau spectra of SN 2014cx at 11600 km s$^{-1}$, possibly a sign of ejecta-circumstellar interaction. The spectra of SN 2014cy exhibit strong absorption profile of H I similar to normal luminosity events whereas strong metal lines akin to sub-luminous SNe. The analytical modelling of the bolometric light curve of the three events yields similar radii for the three objects within errors (478, 507 and 608 R$_\odot$ for SNe 2014cx, 2014cy and 2015cz, respectively) and a range of ejecta masses (15.0, 22.2 and 18.7 M$_\odot$ for SNe 2014cx, 2014cy and 2015cz), and a modest range of explosion energies (3.3 - 6.0 foe where 1 foe = 10$^{51}$ erg).Comment: 20 pages, 23 Figures, 3 figures in appendix, accepted for publication in MNRA