3,113 research outputs found

    Geminivirus C2 protein represses genes involved in sulphur assimilation and this effect can be counteracted by jasmonate treatment

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    Geminiviruses are plant viruses that infect a broad range of crops and cause extensive losses worldwide, having an important economic impact. C2, a multifunctional pathogenicity factor encoded by geminiviruses, has been recently shown to suppress the responses to jasmonates in the host plant, which might at least partially explain its well-established role in pathogenicity. Sulphur is one of the essential macro-elements for plant life, and is considered to have a role in plant defence, in a phenomenon named sulphur-induced resistance (SIR) or sulphur-enhanced defence (SED). In this work, we show that geminivirus C2 protein represses the expression of genes involved in the sulphur assimilation pathway in Arabidopsis, but, interestingly, this effect can be neutralized by exogenous jasmonate treatment. These preliminary results may raise the idea that geminiviruses might be affecting sulphur metabolism, and maybe counteracting SIR/SED, through the manipulation of the jasmonate signalling pathway, which would define a novel strategy in plant-virus interactions and may unveil SIR/SED as an important player in the plant defence against viruses.Ministerio de Ciencia y Innovación/FEDER AGL2007-66062-C02-02/AGR AGL2010-22287-CO2European Regional Development Fund (ERDF) BIO2010-15201Junta de Andalucía BIO­27

    BESDUI: A Benchmark for End-User Structured Data User Interfaces

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    The Semantic Web Community has invested significant research efforts in developing systems for Semantic Web search and exploration. But while it has been easy to assess the systems’ computational efficiency, it has been much harder to assess how well different semantic systems’ user interfaces help their users. In this article, we propose and demonstrate the use of a benchmark for evaluating such user interfaces, similar to the TREC benchmark for evaluating traditional search engines. Our benchmark includes a set of typical user tasks and a well-defined procedure for assigning a measure of performance on those tasks to a semantic system. We demonstrate its application to two such system, Virtuoso and Rhizomer. We intend for this work to initiate a community conversation that will lead to a generally accepted framework for comparing systems and for measuring, and thus encouraging, progress towards better semantic search and exploration tools

    Rejecting abyssal thinking in the language and education of racialized bilinguals: A manifesto

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    Following Boaventura de Sousa Santos, the authors of this article reject the type of “abyssal thinking” that erases the existence of counter-hegemonic knowledges and lifeways, adopting instead the “from the inside out” perspective that is required for thinking constructively about the language and education of racialized bilinguals. On the basis of deep personal experience and extensive field-work research, we challenge prevailing assumptions about language, bilingualism, and education that are based on raciolinguistic ideologies with roots in colonialism. Adopting a translanguaging perspective that rejects rigid colonial boundaries of named languages, we argue that racialized bilingual learners, like all students, draw from linguistic-semiotic, cultural, and historical repertoires. The decolonial approach that guides our work reveals these students making a world by means of cultural and linguistic practices derived from their own knowledge systems. We propose that in order to attain justice and success, a decolonial education must center non-hegemonic modes of “otherwise thinking” by attending to racialized bilinguals’ knowledges and abilities that have always existed yet have continually been distorted and erased through abyssal thinking

    Efecto de las fracciones soluble en insoluble de la fibra de la pulpa de manzana sobre la digestibilidadi ileal y fecal en conejos

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    El objetivo del presente trabajo fue estudiar el efecto de la fibra soluble e insoluble de pulpa de manzana sobre la digestibilidad ileal y fecal en conejos. Se formularon cuatro piensos con niveles similares de fibra insoluble (FND 32,4%) y proteína (18,6%, ambos sobre MS). El nivel de fibra soluble fue bajo en el pienso control (4,6%, su fibra procedió de cascarilla de avena y paja) y aumentó en los piensos con pulpa de manzana depectinizada (7,1%), pulpa de manzana (9,3%) y pectinas de manzana (10,5%). Se determinó la digestibilidad fecal en 23 gazapos/pienso entre los 55 y los 59 d de edad, y se sacrificaron 23 gazapos/pienso a los 60 d de edad para proteger la digesta ileal y determinar la digestibilidad ileal

    Determinación espectrofotométrica de diacetilo en mantequilla previa formación del complejo diacetil diisonicotin hidrazona-Zr(IV)

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    Se ha desarrollado un nuevo método espectrofotométrico para la determinación de diacetilo. Se basa en la formación de diacetildiisonicotin hidrazona por reacción de diacetilo con isoniacida. La hidrazona compleja con Zr (IV) a pH ácido (1,7) formando un quelato amarillo y soluble. La absortividad molar es 1,7.1041.mol·1 cm·1 a 410 nm. El método ha sido aplicado a la determinación de diacetilo en mantequilla.A new spectrophotometric method for determination of diacetyl has been developed. This method make use of the abili ty of diacety 1 to react with isoniazid forming diacetyl diisonicotin hydrazone. This hydrazone then complexes Zr (IV) at acid pH ( 1, 7) giving rise to a yellow and soluble chelate. The molar absorptivity is 1, 7.1041 mol·1 cm·l. Themethodhas been applied tothemeasure of diacetyl in butter

    Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

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    Final grain production and quality in durum wheat are affected by biotic and abiotic stresses. The association mapping (AM) approach is useful for dissecting the genetic control of quantitative traits, with the aim of increasing final wheat production under stress conditions. In this study, we used AM analyses to detect quantitative trait loci (QTL) underlying agronomic and quality traits in a collection of 294 elite durum wheat lines from CIMMYT (International Maize and Wheat Improvement Center), grown under different water regimes over four growing seasons. Thirty-seven significant marker-trait associations (MTAs) were detected for sedimentation volume (SV) and thousand kernel weight (TKW), located on chromosomes 1B and 2A, respectively. The QTL loci found were then confirmed with several AM analyses, which revealed 12 sedimentation index (SDS) MTAs and two additional loci for SV (4A) and yellow rust (1B). A candidate gene analysis of the identified genomic regions detected a cluster of 25 genes encoding blue copper proteins in chromosome 1B, with homoeologs in the two durum wheat subgenomes, and an ubiquinone biosynthesis O-methyltransferase gene. On chromosome 2A, several genes related to photosynthetic processes and metabolic pathways were found in proximity to the markers associated with TKW. These results are of potential use for subsequent application in marker-assisted durum wheat-breeding programs
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