From experience to clinical reporting : challenges of registry in psychoanalytic research

A proposta do artigo é discutir a produção de registros em uma pesquisa psicanalítica. Tal pesquisa realizou o acompanhamento de bebês e educadoras em escolas de educação infantil, e o método de registro construído foi desenvolvido em três tempos. O tempo da experiência referese ao encontro com o campo, englobando exterioridade, alteridade, mas também reflexividade, transformação. O segundo tempo, que foi a escrita do diário clínico, comporta tanto o aspecto subjetivo como a tentativa de descrever cenas, acontecimentos. O terceiro tempo, da composição do relato clínico, envolve teorização metapsicológica, na qual se busca produzir fechamentos e abrir novas perguntas.This paper aims to discuss the production of records in psychoanalytic research. The research carried out an accompanying process of babies and educators in kindergarten schools, and the recording method in question was developed in three stages. The time of experience refers to the encounter with the field, encompassing exteriority, alterity, but also reflexivity, transformation. The second time was the writing of the clinical diary, which includes both the subjective aspect and the attempt to describe scenes and events. The third time, the composition of the clinical narrative report, involves theorizing. It’s when we seek to produce some closings and to uncover new questions

Cinética de secagem do bagaço de uva fermentado : determinação da difusividade efetiva de umidade

The aim of this study was to obtain the equilibrium moisture content of grape (variety ‘Tannat’) pomace through desorption isotherms, to evaluate the drying kinetics, determine the coefficient of effective diffusivity and physico-chemically characterize the grape pomace and the product obtained after drying. The desorption isotherms were determined at 50, 60 and 70 ºC and the experimental data were fitted using the GAB model (Gugghenheim, Anderson and de Boer). Drying was evaluated using a 2² factorial experimental design with three center points and effective diffusivity was obtained through the diffusion model of Fick’s second law. The grape pomace was characterized regarding the contents of moisture, protein, carbohydrates, lipids, ash and dietary crude fiber. The obtained isotherms showed sigmoid shape and the experimental data fitted well to the GAB model. The drying curves showed only a decreasing rate period. The effective diffusivity values were within the range for organic materials. Dry grape pomace showed high contents of protein and fiber and can be used in the development of new products, in order to increase the nutritional content and add value to this byproduct.Propôs-se, neste trabalho, obter a umidade de equilíbrio do bagaço de uva da variedade Tannat por meio das isotermas de dessorção e avaliar a cinética de secagem, além de determinar o coeficiente de difusividade efetiva e caracterizar, físico-quimicamente, o bagaço de uva e o produto obtido pós-secagem. As isotermas de dessorção foram determinadas a 50, 60 e 70 °C e os dados experimentais foram ajustados pelo modelo de GAB (Gugghenheim, Anderson e de Boer). A secagem foi avaliada por meio de um planejamento experimental fatorial 2² com três pontos centrais enquanto a difusividade efetiva foi obtida através do modelo difusivo da segunda Lei de Fick. O bagaço de uva foi caracterizado quanto ao teor de água, proteínas, carboidratos, lipídeos, cinzas e fibra bruta alimentar. As isotermas obtidas apresentaram formato sigmoidal e os dados experimentais se ajustaram bem ao modelo de GAB; as curvas de secagem apresentaram apenas o período de taxa decrescente; os valores da difusividade efetiva se mantiveram dentro da faixa para materiais orgânicos; já o bagaço de uva seco apresentou elevado teor de proteínas e fibras podendo ser inserido no desenvolvimento de novos produtos a fim de elevar o teor nutricional e agregar valor a este subproduto

Molecular epidemiology of heteroresistant vancomycin-intermediate Staphylococcus aureus in Brazil

AbstractTo determine the epidemiological and molecular characteristics of 12 Staphylococcus aureus isolates presenting heteroresistance to vancomycin in laboratories of two cities in Santa Catarina, southern Brazil. Epidemiological data, including the city of isolation, health institution, and date of isolation were considered, as well as the associated clinical specimen. For molecular characterization, we analyzed the staphylococcal cassette chromosome types, the erm gene presence, and the genomic diversity of isolates using pulsed-field gel electrophoresis. The 12 isolates of S. aureus were previously confirmed as heteroresistance to vancomycin using the population analysis profile–area under curve. Regarding genetic variability, two clones were detected: the main one (clone A) composed of four isolates and the clones B, with two isolates. For clone A, two isolates presented identical band patterns and were related to the same hospital, with an interval of 57 days between their isolation. The other isolates of this clone showed no epidemiological link between them because they were isolated in different hospitals and had no temporal relationship. The other clone showed no detectable epidemiological relationship. The heteroresistance to vancomycin recovered in Santa Catarina State from 2009 to 2012 had, in general, heterogeneous genomic patterns based on pulsed-field gel electrophoresis results, which is in accordance with the fact that these isolates had little or no epidemiological relationship among them. Due to the characteristic phenotypic instability and often prolonged vancomycin therapy for selection, clonal spread is not as common as for other resistance mechanisms disseminated through horizontal gene transfer

Two-dimensional radiographs versus cone-beam computed tomography in planning mini-implant placement : a systematic review

This study aimed to compare two-dimensional radiographs and cone beam computed tomography (CBCT) images for mini-implant planning. A search was performed in PubMed, Embase, Web of Science, Cochrane Library and Google Scholar electronic databases accord

Pulsating hydrogen-deficient white dwarfs and pre-white dwarfs observed with TESS : II. Discovery of two new GW Vir stars: TIC 333432673 and TIC 095332541

Context. The Transiting Exoplanet Survey Satellite (TESS) mission is revolutionizing the blossoming area of asteroseismology, particularly of pulsating white dwarfs and pre-white dwarfs, thus continuing the impulse of its predecessor, the Kepler mission. Aims. In this paper, we present the observations from the extended TESS mission in both 120 s short-cadence and 20 s ultra-short cadence mode of two pre-white dwarf stars showing hydrogen deficiency. We identify them as two new GW Vir stars, TIC 333432673 and TIC 095332541. We apply the tools of asteroseismology with the aim of deriving their structural parameters and seismological distances. Methods. We carried out a spectroscopic analysis and a spectral fitting of TIC 333432673 and TIC 095332541. We also processed and analyzed the high-precision TESS photometric light curves of the two target stars, and derived their oscillation frequencies. We performed an asteroseismological analysis of these stars on the basis of PG 1159 evolutionary models that take into account the complete evolution of the progenitor stars. We searched for patterns of uniform period spacings in order to constrain the stellar mass of the stars. We employed the individual observed periods to search for a representative seismological model. Results. The analysis of the TESS light curves of TIC 333432673 and TIC 095332541 reveals the presence of several oscillations with periods ranging from 350 to 500 s associated to typical gravity (g)-modes. From follow-up ground-based spectroscopy, we find that both stars have a similar effective temperature (Teff = 120 000 ± 10 000 K) and surface gravity (log g = 7.5 ± 0.5), but a different He/C composition of their atmosphere. On the basis of PG 1159 evolutionary tracks, we derived a spectroscopic mass of M? = 0.58+0.16 −0.08 M for both stars. Our asteroseismological analysis of TIC 333432673 allowed us to find a constant period spacing compatible with a stellar mass M? ∼ 0.60 − 0.61 M , and an asteroseismological model for this star with a stellar mass M? = 0.589 ± 0.020 M , as well as a seismological distance of d = 459+188 −156 pc. For this star, we find an excellent agreement between the different methods to infer the stellar mass, and also between the seismological distance and that measured with Gaia (dGaia = 389+5.6 −5.2 pc). For TIC 095332541, we have found a possible period spacing that suggests a stellar mass of M? ∼ 0.55−0.57 M . Unfortunately, we have not been able to find an asteroseismological model for this star. Conclusions. Using the high-quality data collected by the TESS space mission and follow-up spectroscopy, we have been able to discover and characterize two new GW Vir stars. The TESS mission is having, and will continue to have, an unprecedented impact on the area of white-dwarf asteroseismology

Pulsating hydrogen-deficient white dwarfs and pre-white dwarfs observed with TESS : II. Discovery of two new GW Vir stars: TIC 333432673 and TIC 095332541

Context. The Transiting Exoplanet Survey Satellite (TESS) mission is revolutionizing the blossoming area of asteroseismology, particularly of pulsating white dwarfs and pre-white dwarfs, thus continuing the impulse of its predecessor, the Kepler mission. Aims. In this paper, we present the observations from the extended TESS mission in both 120 s short-cadence and 20 s ultra-short cadence mode of two pre-white dwarf stars showing hydrogen deficiency. We identify them as two new GW Vir stars, TIC 333432673 and TIC 095332541. We apply the tools of asteroseismology with the aim of deriving their structural parameters and seismological distances. Methods. We carried out a spectroscopic analysis and a spectral fitting of TIC 333432673 and TIC 095332541. We also processed and analyzed the high-precision TESS photometric light curves of the two target stars, and derived their oscillation frequencies. We performed an asteroseismological analysis of these stars on the basis of PG 1159 evolutionary models that take into account the complete evolution of the progenitor stars. We searched for patterns of uniform period spacings in order to constrain the stellar mass of the stars. We employed the individual observed periods to search for a representative seismological model. Results. The analysis of the TESS light curves of TIC 333432673 and TIC 095332541 reveals the presence of several oscillations with periods ranging from 350 to 500 s associated to typical gravity (g)-modes. From follow-up ground-based spectroscopy, we find that both stars have a similar effective temperature (Teff = 120 000 ± 10 000 K) and surface gravity (log g = 7.5 ± 0.5), but a different He/C composition of their atmosphere. On the basis of PG 1159 evolutionary tracks, we derived a spectroscopic mass of M? = 0.58+0.16 −0.08 M for both stars. Our asteroseismological analysis of TIC 333432673 allowed us to find a constant period spacing compatible with a stellar mass M? ∼ 0.60 − 0.61 M , and an asteroseismological model for this star with a stellar mass M? = 0.589 ± 0.020 M , as well as a seismological distance of d = 459+188 −156 pc. For this star, we find an excellent agreement between the different methods to infer the stellar mass, and also between the seismological distance and that measured with Gaia (dGaia = 389+5.6 −5.2 pc). For TIC 095332541, we have found a possible period spacing that suggests a stellar mass of M? ∼ 0.55−0.57 M . Unfortunately, we have not been able to find an asteroseismological model for this star. Conclusions. Using the high-quality data collected by the TESS space mission and follow-up spectroscopy, we have been able to discover and characterize two new GW Vir stars. The TESS mission is having, and will continue to have, an unprecedented impact on the area of white-dwarf asteroseismology

Human-derived NLS enhance the gene transfer efficiency of chitosan

Nuclear import is considered as one of the major limitations for non-viral gene delivery systems and the incorporation of nuclear localization signals (NLS) that mediate nuclear intake can be used as a strategy to enhance internalization of exogenous DNA. In this work, human-derived endogenous NLS peptides based on insulin growth factor binding proteins (IGFBP), namely IGFBP-3 and IGFBP-5, were tested for their ability to improve nuclear translocation of genetic material by non-viral vectors. Several strategies were tested to determine their effect on chitosan mediated transfection efficiency: co-administration with polyplexes, co-complexation at the time of polyplex formation, and covalent ligation to chitosan. Our results show that co-complexation and covalent ligation of the NLS peptide derived from IGFBP-3 to chitosan polyplexes yields a 2-fold increase in transfection efficiency, which was not observed for NLS peptide derived from IGFBP-5. These results indicate that the integration of IGFBP-NLS-3 peptides into polyplexes has potential as a strategy to enhance the efficiency of non-viral vectors.FCT: PTDC/BTM/ORG/28121/2017; PD/BD/52424/2013; SFRH/BD/76873/2011;PIRG-GA-2009-249314info:eu-repo/semantics/publishedVersio