Engineering enzymes for green manufacturing of noncanonical amino acids

Please click Additional Files below to see the full abstract

Risk and resiliency factors in posttraumatic stress disorder

BACKGROUND: Not everyone who experiences a trauma develops posttraumatic stress disorder (PTSD). The aim of this study was to determine the risk and resiliency factors for this disorder in a sample of people exposed to trauma. METHOD: Twenty-five people who had developed PTSD following a trauma and 27 people who had not were asked to complete the Posttraumatic Stress Diagnostic Scale, the Coping Inventory for Stressful Situations, and the State-Trait Anxiety Inventory. In addition, they completed a questionnaire to provide information autobiographic and other information. ANALYSIS: Five variables that discriminated significantly between the two groups using chi-square analysis or t-tests were entered into a logistic regression equation as predictors, namely, being female, perceiving a threat to one's life, having a history of sexual abuse, talking to someone about the event, and the "intentionality" of the trauma. RESULTS: Only being female and perceiving a threat to one's life were significant predictors of PTSD. Taking base rates into account, 96.0% of participants with PTSD were correctly classified as having the disorder and 37.0% of participants without PTSD were correctly classified as not having the disorder, for an overall success rate of 65.4% CONCLUSIONS: Because women are more likely than men to develop PTSD, more preventive measures should be directed towards them. The same is true for trauma victims (of both sexes) who feel that their life was in dange

The Impact of Utah 4-H on Positive Youth Development

4-H is the largest youth development program in the United States. Utah 4-H conducts programs guided by the principles of positive youth development to help youth across the state lead a successful life. This article discusses the impact of Utah 4-H on the wellbeing of youth

Anti-Americanism in Arabic Twitter discourses is driven by perceptions of U.S. impingement in the region.

Recent years have seen very public expressions of anti-American sentiment in many parts of the Middle East. But are these sentiments driven by American actions in the region, or by the country itself? In new research, Amaney A. Jamal, Robert O. Keohane, David Romney, and Dustin Tingley use data from Arabic Twitter from 2012 and 2013 to investigate anti-American attitudes. By looking at reactions to the potential for U.S. intervention in situations such as the Syrian Civil War, they find that in the Arabic Twitter world, negative attitudes are driven by the perceptions of potential American social and political impingement. They also find that these negative attitudes can be directed at other impinging powers, such as Iran

Radio Jet-Ambient Medium Interactions on Parsec Scales in the Blazar 1055+018

As part of our study of the magnetic fields of AGN we have recently observed a large sample of blazars with the Very Long Baseline Array. Here we report the discovery of a striking two-component jet in the source 1055+018, consisting of an inner spine with a transverse magnetic field, and a fragmentary but distinct boundary layer with a longitudinal magnetic field. The polarization distribution in the spine strongly supports shocked-jet models while that in the boundary layer suggests interaction with the surrounding medium. This behavior suggests a new way to understand the differing polarization properties of strong- and weak-lined blazars.Comment: LaTex; 10 pages; 6 figures; reference fix; to appear in ApJL, 518, 1999 June 2

Improved Livelihoods from Grasslands; the Case of Napier Grass in Smallholder Dairy Farms in Kenya

In Kenya, smallholder farmers produce about 80% of the marketed milk. The farming systems vary from mixed farms with up to 10 ha of land and \u3c 10 dairy cows (Gitau et al., 1994; Anon., 1985), to intensive smallholder dairy producers in the high human population central Kenya region with 0.9 to 2 ha of land and 3-4 dairy cows (Staal et al., 2001a). Milk production depends heavily on the cultivation of forages, with Pennisetum purpureum (Napier grass) by far the most important. An estimated 350,000 of the 600,000 smallholder farms in Kenya grow and utilize P. purpureum on their farms. There are various published data indicating the level of dependence on sown forages. In a survey of 21 smallholder dairy farmers in the highlands of Kenya, Romney et al. (2004) found that P. purpureum supplied approximately 40 and 60% of the feed offered to dairy cows in the dry and wet months respectively, with the remaining feed provided by concentrates, crop residues (mainly Zea mays (maize) stover) and other cut and carry fodder such as roadside grass. In the more intensive cut and carry systems of production practiced in central Kenya, McLeod et al. (2003) found that P. purpureum was grown by over 70% of the smallholder farmers in their study area. In farm level characterisation surveys of over 3300 households conducted between 1996 and 2000 in central Kenya, 62% kept livestock and more than 50% were growing P. purpureum. Farmers were also growing fodder legumes such as Sesbania grandiflora (Sesbania), Leucaena leucocephala (Leucaena), Calliandra calothyrsus (Callindra), Desmodium intortum/uncinatum, (Desmodium) and Medicago sativa (Lucerne), but the frequency did not exceed 7.5% (Staal et al., 2001b)

Enantioselective Total Synthesis of Nigelladine A via Late-Stage C–H Oxidation Enabled by an Engineered P450 Enzyme

An enantioselective total synthesis of the norditerpenoid alkaloid nigelladine A is described. Strategically, the synthesis relies on a late-stage C–H oxidation of an advanced intermediate. While traditional chemical methods failed to deliver the desired outcome, an engineered cytochrome P450 enzyme was employed to effect a chemo- and regioselective allylic C–H oxidation in the presence of four oxidizable positions. The enzyme variant was readily identified from a focused library of three enzymes, allowing for completion of the synthesis without the need for extensive screening

Meals described as healthy or unhealthy match public health education in England

Dietary guidelines for the general public aim to lower the incidence of nutrition-related diseases by influencing habitual food choices. Yet little is known about how well the guidelines are matched by the actual practices that people regard as healthy or unhealthy. In the present study, British residents were asked in a cognitive interview to write a description of an occasion when either they ate in an unhealthy way or the eating was healthy. The reported foods and drinks, as well as sort of occasion, location, people present and time of day, were categorised by verbal and semantic similarities. The number of mentions of terms in each category was then contrasted between groups in exact probability tests. Perceived unhealthy and healthy eating occasions differed reliably in the sorts of foods and the contexts reported. There was also full agreement with the national guidelines on eating plenty of fruit and vegetables, eating small amounts of foods and drinks high in fat and/or sugar, drinking plenty of water, and cutting down on alcohol. There was a tendency to regard choices of bread, rice, potatoes, pasta and other starchy foods as healthy. Reported healthy and unhealthy eating did not differ in incidences of meat, fish, eggs, beans and other non-dairy sources of protein or of dairy foods and milk. These results indicate that operationally clear recommendations by health professionals are well understood in this culture but members of the public do not make clear distinctions in the case of foods that can be included in moderate amounts in a healthy diet