Optical binding in nanoparticle assembly: Potential energy landscapes

Optical binding is an optomechanical effect exhibited by systems of micro- and nanoparticles, suitably irradiated with off-resonance laser light. Physically distinct from standing-wave and other forms of holographic optical traps, the phenomenon arises as a result of an interparticle coupling with individual radiation modes, leading to optically induced modifications to Casmir-Polder interactions. To better understand how this mechanism leads to the observed assemblies and formation of patterns in nanoparticles, we develop a theory in terms of optically induced energy landscapes exhibiting the three-dimensional form of the potential energy field. It is shown in detail that the positioning and magnitude of local energy maxima and minima depend on the configuration of each particle pair, with regards to the polarization and wave vector of the laser light. The analysis reveals how the positioning of local minima determines the energetically most favorable locations for the addition of a third particle to each equilibrium pair. It is also demonstrated how the result of such an addition subtly modifies the energy landscape that will, in turn, determine the optimum location for further particle additions. As such, this development represents a rigorous and general formulation of the theory, paving the way toward full comprehension of nanoparticle assembly based on optical binding

Virulence- and signaling-associated genes display a preference for long 3′UTRs during rice infection and metabolic stress in the rice blast fungus

Generation of mRNA isoforms by alternative polyadenylation (APA) and their involvement in regulation of fungal cellular processes, including virulence, remains elusive. Here, we investigated genome‐wide polyadenylation site (PAS) selection in the rice blast fungus to understand how APA regulates pathogenicity. More than half of Magnaporthe oryzae transcripts undergo APA and show novel motifs in their PAS region. Transcripts with shorter 3′UTRs are more stable and abundant in polysomal fractions, suggesting they are being translated more efficiently. Importantly, rice colonization increases the use of distal PASs of pathogenicity genes, especially those participating in signalling pathways like 14‐3‐3B, whose long 3′UTR is required for infection. Cleavage factor I (CFI) Rbp35 regulates expression and distal PAS selection of virulence and signalling‐associated genes, tRNAs and transposable elements, pointing its potential to drive genomic rearrangements and pathogen evolution. We propose a noncanonical PAS selection mechanism for Rbp35 that recognizes UGUAH, unlike humans, without CFI25. Our results showed that APA controls turnover and translation of transcripts involved in fungal growth and environmental adaptation. Furthermore, these data provide useful information for enhancing genome annotations and for cross‐species comparisons of PASs and PAS usage within the fungal kingdom and the tree of life

Bases de poder del supervisor, conducta cooperadora y compromiso con la organización

Este trabajo analiza, en el contexto español, las bases de poder de los supervisores y la incidencia que las mismas tienen sobre la conducta cooperadora y el compromiso con la organización de sus subordinados. Los datos analizados pertenecen a 335 sujetos con la menos un año de experiencia laboal a tiempo completo. Mediante la contrastación de un modelo estructural, que relaciona las diversas bases de poder del jefe entre sí y con la conducta cooperadora y el grado de compromiso de los subordinados, se tratan de comprobar las relaciones existentes entre las variables mencionadas. Asimismo, se indican los principales resultados alcanzados y las implicaciones más relevantes que se pueden deducir a partir de los mismos de cara a las prácticas de los directivos

Anaerobic Thermophilic Colonization of Porous Support

Biofilm development in an open-pore sintered glass material (SIRAN) was studied using a laboratory-scale anaerobic fixed-film bioreactor under thermophilic conditions. The startup and performance of that bioreactor, operating on distillery waste water feed (vinasses), were also studied. Results obtained indicated that stepped organic loading during initial bioreactor start-up reduced the periods of adaptation in the colonization process and micro-organism attachment and biofilm formation was accelerated by the surface characteristics of the carrier. The results obtained by operating with stepped organic loading (& = 2.0 kg m–3 d–1 COD) over a period of 70 days suggest that a stable operation of the process (90% COD removal) and high density of biomass immobilized on the support (20 kgVSatt per m3 SIRAN) is achieved. Epifluorescence microscopy demonstrated that, initially, attached growth was developed in crevices, where biomass was protected from shear forces and, finally, SIRAN was completely covered and biofilm developed on the entire SIRAN particles

A retarded coupling approach to intermolecular interactions

A wide range of physical phenomena such as optical binding and resonance energy transfer involve electronic coupling between adjacent molecules. A quantum electrodynamical description of these intermolecular interactions reveals the presence of retardation effects. The clarity of the procedure associated with the construction of the quantum amplitudes and the precision of the ensuing results for observable energies and rates are widely acknowledged. However, the length and complexity of the derivations involved in such quantum electrodynamical descriptions increase rapidly with the order of the process under study. Whether through the use of time-ordering approaches, or the more expedient state-sequence method, time-consuming calculations cannot usually be bypassed. A simple and succinct method is now presented, which provides for a direct and still entirely rigorous determination of the quantum electrodynamical amplitudes for processes of arbitrarily high order. Using the approach, new results for optical binding in two- and three-particle systems are secured and discussed

Influence of the state of light on the optically induced interparticle interaction

A general expression for the energy of interparticle interaction induced by an arbitrary mode of light is determined using quantum electrodynamics, and it is shown that the Casimir-Polder potential is included within this quantum result. Equations are also derived for the corresponding coupling induced by multimode number states of light, and the dependence of the pair energy on the Poynting vector and polarization state is determined. Attention is then focused on the interactions between particles trapped in counterpropagating coherent beams, both with and without interference, and it is shown that the results afford insights into the multiparticle structures that can be optically fabricated with counterpropagating input. Brief consideration is also given to the effect of squeezing the optical coherent state. Extending previous studies of optical binding in Laguerre-Gaussian beams, results are given for the case of particles trapped at radially different locations within the beam structure. Finally, consideration is given to interparticle interactions induced by broadband light, and it is shown how the length of optically fabricated particle chains can be controlled by the use of wavelength filters

Cathodoluminescence study of ZnO wafers cut from hydrothermal crystals

ZnO is a wide bandgap semiconductor with very promising expectation for UV optoelectronics. The existence of large crystals should allow homoepitaxial growth of ZnO films for advanced optoelectronic devices. However, the ZnO substrates are not yet mature. Both defect induced by growth and by polishing together with the high reactivity of the surface are problems to their industrial application. Cathodoluminescence (CL) was used to probe the quality of substrates from two different suppliers. The surface damage was studied by varying the penetration depth of the electron beam, allowing to observe significant differences between the two samples within a 0.5-mm-thick surface layer. CL spectra show a complex band (P1) at _3.3 eV composed of two overlapped bands (3.31 and 3.29 eV) related to point defects (PD) and the 1-LO phonon replica of the free exciton (FX-1LO). This band (P1) is shown to be very sensitive to the presence of defects and the surface and thermal treatments. Its intensity compared with the excitonic band intensity is demonstrated to provide criteria about the quality of the substrates

Jumping-based asymmetries are negatively associated with jump, change of direction, and repeated sprint performance, but not linear speed, in adolescent handball athletes

The aim of the present study was to determine the association of multi-directional jumping asymmetries with measures of physical performance. Forty-two youth handball athletes (age: 16.0 ± 1.3 years; body height: 174.11 ± 7.3 cm; body mass: 70.49 ± 13.3 kg) performed a mid-season fitness test battery consisting of single leg countermovement, lateral and broad jump tests, two change of direction speed (CODS) tests, an 8 x 10 m repeated sprint test, and a 20 m sprint. The Kappa coefficient showed only ‘slight’ levels of agreement (K range = -0.05 to 0.15), indicating that asymmetries rarely favoured the same side during each of the jump tests. The single leg countermovement jump showed significantly (p = 0.006) larger asymmetries (11.2 ± 8.4) than the broad jump (6.4 ± 4.6) and significant correlations were present between jumping asymmetries and jump (r = -0.32 to -0.52), CODS (r = 0.31 to 0.32) and repeated sprint (r = 0.35 to 0.40) performance. The findings of the present study highlight the independent nature of jumping asymmetries and associations with measures of physical performance. Practitioners are encouraged to use multiple tests to detect existing side differences and consider appropriate training interventions for the reduction of inter-limb asymmetries

On the monitoring of surface displacement in connection with volcano reactivation in Tenerife, Canary Islands, using space techniques

Geodetic volcano monitoring in Tenerife has mainly focused on the Las Cañadas Caldera, where a geodetic micronetwork and a levelling profile are located. A sensitivity test of this geodetic network showed that it should be extended to cover the whole island for volcano monitoring purposes. Furthermore, InSAR allowed detecting two unexpected movements that were beyond the scope of the traditional geodetic network. These two facts prompted us to design and observe a GPS network covering the whole of Tenerife that was monitored in August 2000. The results obtained were accurate to one centimetre, and confirm one of the deformations, although they were not definitive enough to confirm the second one. Furthermore, new cases of possible subsidence have been detected in areas where InSAR could not be used to measure deformation due to low coherence. A first modelling attempt has been made using a very simple model and its results seem to indicate that the deformation observed and the groundwater level variation in the island may be related. Future observations will be necessary for further validation and to study the time evolution of the displacements, carry out interpretation work using different types of data (gravity, gases, etc) and develop models that represent the island more closely. The results obtained are important because they might affect the geodetic volcano monitoring on the island, which will only be really useful if it is capable of distinguishing between displacements that might be linked to volcanic activity and those produced by other causes. One important result in this work is that a new geodetic monitoring system based on two complementary techniques, InSAR and GPS, has been set up on Tenerife island. This the first time that the whole surface of any of the volcanic Canary Islands has been covered with a single network for this purpose. This research has displayed the need for further similar studies in the Canary Islands, at least on the islands which pose a greater risk of volcanic reactivation, such as Lanzarote and La Palma, where InSAR techniques have been used already

Effects of 8-weeks of isoinertial vs. cable-resistance training on motor skills performance and interlimb asymmetries

Two training interventions conducted on youth handball athletes