Probability distributions of smeared quantum stress tensors

We obtain in closed form the probability distribution for individual measurements of the stress-energy tensor of two-dimensional conformal field theory in the vacuum state, smeared in time against a Gaussian test function. The result is a shifted Gamma distribution with the shift given by the previously known optimal quantum inequality bound. For small values of the central charge it is overwhelmingly likely that individual measurements of the sampled energy density in the vacuum give negative results. For the case of a single massless scalar field, the probability of finding a negative value is 84%. We also report on computations for four-dimensional massless scalar fields showing that the probability distribution of the smeared square field is also a shifted Gamma distribution, but that the distribution of the energy density is not.Comment: 9 pages, 1 figure. Minor edits implemente

Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding

The MAGE (melanoma associated antigen) protein family are tumour-associated proteins normally present only in reproductive tissues such as germ cells of the testis. The human genome encodes over 60 MAGE genes of which one class (containing MAGE-A3 and MAGE-A4) are exclusively expressed in tumours, making them an attractive target for the development of targeted and immunotherapeutic cancer treatments. Some MAGE proteins are thought to play an active role in driving cancer, modulating the activity of E3 ubiquitin ligases on targets related to apoptosis. Here we determined the crystal structures of MAGE- A3 and MAGE-A4. Both proteins crystallized with a terminal peptide bound in a deep cleft between two tandem-arranged winged helix domains. MAGE-A3 (but not MAGE-A4), is pre- dominantly dimeric in solution. Comparison of MAGE-A3 and MAGE-A3 with a structure of an effector-bound MAGE-G1 suggests that a major conformational rearrangement is required for binding, and that this conformational plasticity may be targeted by allosteric binders

On wormholes with arbitrarily small quantities of exotic matter

Recently several models of traversable wormholes have been proposed which require only arbitrarily small amounts of negative energy to hold them open against self-collapse. If the exotic matter is assumed to be provided by quantum fields, then quantum inequalities can be used to place constraints on the negative energy densities required. In this paper, we introduce an alternative method for obtaining constraints on wormhole geometries, using a recently derived quantum inequality bound on the null-contracted stress-energy averaged over a timelike worldline. The bound allows us to perform a simplified analysis of general wormhole models, not just those with small quantities of exotic matter. We then use it to study, in particular, the models of Visser, Kar, and Dadhich (VKD) and the models of Kuhfittig. The VKD models are constrained to be either submicroscopic or to have a large discrepancy between throat size and curvature radius. A recent model of Kuhfittig is shown to be non-traversable. This is due to the fact that the throat of his wormhole flares outward so slowly that light rays and particles, starting from outside the throat, require an infinite lapse of affine parameter to reach the throat.Comment: 30 pages, 2 figure

Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice

We have examined the regulatory role of the individual components of the immunoglobulin antigen receptor in B-cell development by transgenic complementation of Rag-1 deficient (Rag-1⁻) mice. Complementation with a membrane µ heavy chain (µHC) gene allows progression of developmentally arrested Rag-1⁻ pro-B-cells to the small pre-B cell stage, whereas the introduction of independently integrated µHC and κ light chain (κLC) transgenes promotes the appearance of peripheral lymphocytes which, however, remain unresponsive to external stimuli. Complete reconstitution of the B-cell lineage and the emergence of functionally nature Rag-1⁻ peripheral B cells is achieved by the introduction of cointegrated heavy and light chain transgenes encoding an anti-H-2^k antibody. This experimental system demonstrates the competence of the µHC and κLC to direct and regulate the sequential stages of B-cell differentiation, defines the time at which negative selection of self-reactive B cells occurs, and shows that elimination of these cells occurs equally well in the absence of Rag-1 as in its presence. These data also support the hypothesis that Rag-1 directly participates in the V(D)J recombination process

Averaged Energy Inequalities for the Non-Minimally Coupled Classical Scalar Field

The stress energy tensor for the classical non-minimally coupled scalar field is known not to satisfy the point-wise energy conditions of general relativity. In this paper we show, however, that local averages of the classical stress energy tensor satisfy certain inequalities. We give bounds for averages along causal geodesics and show, e.g., that in Ricci-flat background spacetimes, ANEC and AWEC are satisfied. Furthermore we use our result to show that in the classical situation we have an analogue to the phenomenon of quantum interest. These results lay the foundations for analogous energy inequalities for the quantised non-minimally coupled fields, which will be discussed elsewhere.Comment: 8 pages, RevTeX4. Minor typos corrected; version to appear in Phys Rev

Mechanosensing of shear by Pseudomonas aeruginosa leads to increased levels of the cyclic-di-GMP signal initiating biofilm development

Biofilms are communities of sessile microbes that are phenotypically distinct from their genetically identical, free-swimming counterparts. Biofilms initiate when bacteria attach to a solid surface. Attachment triggers intracellular signaling to change gene expression from the planktonic to the biofilm phenotype. For Pseudomonas aeruginosa, it has long been known that intracellular levels of the signal cyclic-di-GMP increase upon surface adhesion and that this is required to begin biofilm development. However, what cue is sensed to notify bacteria that they are attached to the surface has not been known. Here, we show that mechanical shear acts as a cue for surface adhesion and activates cyclic-di-GMP signaling. The magnitude of the shear force, and thereby the corresponding activation of cyclic-di-GMP signaling, can be adjusted both by varying the strength of the adhesion that binds bacteria to the surface and by varying the rate of fluid flow over surface-bound bacteria. We show that the envelope protein PilY1 and functional type IV pili are required mechanosensory elements. An analytic model that accounts for the feedback between mechanosensors, cyclic-di-GMP signaling, and production of adhesive polysaccharides describes our data well

Intra-arterial Onyx Embolization of Vertebral Body Lesions

While Onyx embolization of cerebrospinal arteriovenous shunts is well-established, clinical researchers continue to broaden applications to other vascular lesions of the neuraxis. This report illustrates the application of Onyx (eV3, Plymouth, MN) embolization to vertebral body lesions, specifically, a vertebral hemangioma and renal cell carcinoma vertebral body metastatic lesion

Probability distributions for quantum stress tensors in four dimensions

We treat the probability distributions for quadratic quantum fields, averaged with a Lorentzian test function, in four-dimensional Minkowski vacuum. These distributions share some properties with previous results in two-dimensional spacetime. Specifically, there is a lower bound at a finite negative value, but no upper bound. Thus arbitrarily large positive energy density fluctuations are possible. We are not able to give closed form expressions for the probability distribution, but rather use calculations of a finite number of moments to estimate the lower bounds, the asymptotic forms for large positive argument, and possible fits to the intermediate region. The first 65 moments are used for these purposes. All of our results are subject to the caveat that these distributions are not uniquely determined by the moments. However, we also give bounds on the cumulative distribution function that are valid for any distribution fitting these moments.We apply the asymptotic form of the electromagnetic energy density distribution to estimate the nucleation rates of black holes and of Boltzmann brains.Comment: 26 pages, 2 figure

Neurocysticercosis in Oregon, 1995–2000

The unexpected death of a teenager from neurocysticercosis prompted an investigation of this disease in Oregon. We found 89 hospitalizations, 43 newly diagnosed cases, and 6 deaths from 1995 to 2000. At least five cases occurred in persons who had not traveled or lived outside the United States. Enhanced surveillance for neurocysticercosis is warranted

Progress in achieving quantitative classification of psychopathology

Shortcomings of approaches to classifying psychopathology based on expert consensus have given rise to contemporary efforts to classify psychopathology quantitatively. In this paper, we review progress in achieving a quantitative and empirical classification of psychopathology. A substantial empirical literature indicates that psychopathology is generally more dimensional than categorical. When the discreteness versus continuity of psychopathology is treated as a research question, as opposed to being decided as a matter of tradition, the evidence clearly supports the hypothesis of continuity. In addition, a related body of literature shows how psychopathology dimensions can be arranged in a hierarchy, ranging from very broad "spectrum level" dimensions, to specific and narrow clusters of symptoms. In this way, a quantitative approach solves the "problem of comorbidity" by explicitly modeling patterns of co-occurrence among signs and symptoms within a detailed and variegated hierarchy of dimensional concepts with direct clinical utility. Indeed, extensive evidence pertaining to the dimensional and hierarchical structure of psychopathology has led to the formation of the Hierarchical Taxonomy of Psychopathology (HiTOP) Consortium. This is a group of 70 investigators working together to study empirical classification of psychopathology. In this paper, we describe the aims and current foci of the HiTOP Consortium. These aims pertain to continued research on the empirical organization of psychopathology; the connection between personality and psychopathology; the utility of empirically based psychopathology constructs in both research and the clinic; and the development of novel and comprehensive models and corresponding assessment instruments for psychopathology constructs derived from an empirical approach