Evolução biológica nos livros didáticos de Biologia do ensino médio

Resultados parciais de pesquisa de mestrado em andamento, numa abordagem qualitativa, que visou analisar como está estruturada a evolução biológica nos livros didáticos de Biologia do Ensino Médio aprovados pelo Programa Nacional do Livro Didático para o Ensino Médio (PNLEM 2007/2009). Foram analisadas oito das nove obras aprovadas. Investigou-se como está distribuído o tema ao longo dos livros. O quadro teórico construído baseou-se em trabalhos sobre a evolução biológica tanto no contexto científico quanto no escolar; assim como pesquisas em educação tendo como objeto de estudo os livros didáticos. A tendência à organização disciplinar e a possibilidade de diversas abordagens são algumas de nossas discussões

HB 359 - Power of Attorney

The bill would have repealed and replaced Georgia’s Power of Attorney for the Care of a Minor Child Act. The category of people who could be given power of attorney for the care of a minor child would have expanded from only grandparents and great-grandparents to a broad category of the child’s relatives, and anyone associated with a non-profit organization focused on child or family services or a licensed child-placing agency

Histological correlation of nasal mass: a five year retrospective and prospective study

Background:The presence of mass in the nose may seem to be a simple problem; however it raises several issues about the differential diagnosis. The aim of this study is to evaluate the histological profile of nasal masses in the environment.Methods:This is a retrospective and prospective study of nasal masses during a period of five years of patients presenting to the clinician in our hospital.Results:A total of 90 patients were analysed age ranging from 3 to 80 years. Majority of patients were in the second decade. Sex wise distribution showed a male to female ratio of 2.2:1. The main presenting symptom was nasal obstruction seen in 53.30% patients, while proptosis was seen only in malignant lesion. Among a total of 90 lesions in the nasal cavity 81.1% were benign, 15.5 % were malignant and 3.3% were inadequate sample The commonest non neoplastic lesion was nasal polyp which comprised about 83.33% followed by rhinoscleroma, while the commonest benign neoplastic lesion was papilloma and comprised about 42.10% and squamous cell carcinoma was the most common malignant neoplastic lesion in the nasal cavity and comprised about 42.85%. Most patients with benign lesion were in the second decade. Malignant lesions occurred mainly after fifth decade, while inadequacy of sample was seen in the second decade.  Conclusion: Males are more commonly affected then females. Nasal obstruction is the commonest symptom Simple inflammatory nasal polyp is still the commonest histological pattern seen while papilloma being the most common benign lesion and squamous cell carcinoma the most common malignant lesion.

Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles

FCT/MCTES - UID/Multi/04378/2019. FCT/MCTES - SFRH/BPD/124612/2016.Purpose: Progression of chronic myeloid leukemia (CML) is frequently associated with increased angiogenesis at the bone marrow mediated by exosomes. The capability of gold nanoparticles (AuNPs) functionalized with antiangiogenic peptides to hinder the formation of new blood vessels has been demonstrated in a chorioallantoic membrane (CAM) model. Methods: Exosomes of K562 CML cell line were isolated and their angiogenic effect assessed in a CAM model. AuNPs functionalized with antiangiogenic peptides were used to block the angiogenic effect of CML-derived exosomes, assessed by evaluation of expression levels of key modulators involved in angiogenic pathways - VEGFA, VEGFR1 (also known as FLT1) and IL8. Results: Exosomes isolated from K562 cells promoted the doubling of newly formed vessels associated with the increase of VEGFR1 expression. This is a concentration and timedependent effect. The AuNPs functionalized with antiangiogenic peptides were capable to block the angiogenic effect by modulating VEGFR1 associated pathway. Conclusion: Exosomes derived from blast cells are capable to trigger (neo)-angiogenesis, a key factor for the progression and spreading of cancer, in particular in CML. AuNPs functionalized with specific antiangiogenic peptides are capable to block the effect of the exosomes produced by malignant cells via modulation of the intrinsic VEGFR pathway. Together, these data highlight the potential of nanomedicine-based strategies against cancer proliferation.publishersversionpublishe

Exploring RAB11A Pathway to Hinder Chronic Myeloid Leukemia-Induced Angiogenesis In Vivo

project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. This work is also funded by Fundação para a Ciência e Tecnologia in the scope of the project 2022.04315.PTDC. Publisher Copyright: © 2023 by the authors.Neoangiogenesis is generally correlated with poor prognosis, due to the promotion of cancer cell growth, invasion and metastasis. The progression of chronic myeloid leukemia (CML) is frequently associated with an increased vascular density in bone marrow. From a molecular point of view, the small GTP-binding protein Rab11a, involved in the endosomal slow recycling pathway, has been shown to play a crucial role for the neoangiogenic process at the bone marrow of CML patients, by controlling the secretion of exosomes by CML cells, and by regulating the recycling of vascular endothelial factor receptors. The angiogenic potential of exosomes secreted by the CML cell line K562 has been previously observed using the chorioallantoic membrane (CAM) model. Herein, gold nanoparticles (AuNPs) were functionalized with an anti-RAB11A oligonucleotide (AuNP@RAB11A) to downregulate RAB11A mRNA in K562 cell line which showed a 40% silencing of the mRNA after 6 h and 14% silencing of the protein after 12 h. Then, using the in vivo CAM model, these exosomes secreted by AuNP@RAB11A incubated K562 did not present the angiogenic potential of those secreted from untreated K562 cells. These results demonstrate the relevance of Rab11 for the neoangiogenesis mediated by tumor exosomes, whose deleterious effect may be counteracted via targeted silencing of these crucial genes; thus, decreasing the number of pro-tumoral exosomes at the tumor microenvironment.publishersversionpublishe

Application of Precision Agriculture for the Sustainable Management of Fertilization in Olive Groves

Olive tree growing (Olea europaea L.) has considerably increased in the last decades, as has the consumption of extra virgin olive oil in the world. Precision agriculture is increasingly being applied in olive orchards as a new method to manage agronomic variability with the aim of providing individual plants with the right input amount, limiting waste or excess. The objective of this study was to develop a methodology on a GIS platform using GEOBIA algorithms in order to build prescription maps for variable rate (VRT) nitrogen fertilizers application in an olive orchard. The fertilization plan was determined for each tree by applying its own nitrogen balance, taking into account the variability of nitrogen in soil, leaf, production, and actual biometric and spectral conditions. Each olive tree was georeferenced using the S7-G Stonex instrument with real-time kinematic RTK positioning correction and the trunk cross section area (TCSA) was measured. Soil and leaves were sampled to study nutrient variability. Soil and plant samples were analyzed for all major physical and chemical properties. Spectral data were obtained using a multispectral camera (DJI multispectral) carried by an unmanned aerial vehicle (UAV) platform (DJI Phantom4). The biometric characteristics of the plants were extracted from the achieved normalized vegetation index (NDVI) map. The obtained prescription map can be used for variable rate fertilization with a tractor and fertilizer spreader connected via the ISOBUS system. Using the proposed methodology, the variable rate application of nitrogen fertilizer resulted in a 31% reduction in the amount to be applied in the olive orchard compared to the standard dose

MULTIPLE CUTANEOUS PILOLEIOMYOMA WITH UTERINE FIBROIDS (REED\u27S SYNDROME): A RARE CASE REPORT

Cutaneous leiomyomas comprise approximately 5% of all leiomyomas. Solitary occurrence is more common than generalized. Multiple cutaneous piloleiomyomas are rare, painful and difficult-to-treat benign tumors originating in the arrectores pilorum muscles of the hair follicles. Multiple Cutaneous piloleiomyomas are associated with uterine fibroids in women (Reed\u27s syndrome) and it is a marker of a condition called hereditary leiomyomatosis and renal cell cancer (HLRCC). We report a case of multiple cutaneous piloleiomyoma with uterine fibroid in an adult female. Because of its rarity and association with renal cell cancer, it deserves a mention in literature. KEYWORDS: Cutaneous leiomyoma; Uterine leiomyoma; Painful nodules

MULTIPLE CUTANEOUS PILOLEIOMYOMA WITH UTERINE FIBROIDS (REED'S SYNDROME): A RARE CASE REPORT

Cutaneous leiomyomas comprise approximately 5% of all leiomyomas. Solitary occurrence is more common than generalized. Multiple cutaneous piloleiomyomas are rare, painful and difficult-to-treat benign tumors originating in the arrectores pilorum muscles of the hair follicles. Multiple Cutaneous piloleiomyomas are associated with uterine fibroids in women (Reed's syndrome) and it is a marker of a condition called hereditary leiomyomatosis and renal cell cancer (HLRCC). We report a case of multiple cutaneous piloleiomyoma with uterine fibroid in an adult female. Because of its rarity and association with renal cell cancer, it deserves a mention in literature. KEYWORDS: Cutaneous leiomyoma; Uterine leiomyoma; Painful nodules