Inspiratory Muscle Strength and Cardiorespiratory Fitness Association With Health-Related Quality of Life in Healthy Older Adults

The main purpose of this study was to explore similarities and differences in the association between two capabilities affecting the cardiorespiratory system (overall and multifactorial cardiorespiratory fitness and inspiratory muscle strength) and the health-related quality of life (HRQoL), in a group of active healthy seniors. Sixty-five individuals (age, 73.01 ± 5.27 years; 53 women) who participated regularly in a multicomponent training program completed the EuroQol 5D-5L questionnaire, the 6-min walking test (6MWT), and the maximum inspiratory pressure test (MIP). Non-parametric correlations (Spearman's rho) were conducted to analyze the association between HRQoL indices (EQindex and EQvas), MIP, and 6MWT, considering both, the whole sample and men and women separately. Furthermore, partial correlation was made by controlling age and sex. We found a moderate association between HRQoL and cardiorespiratory fitness (EQvas: r = 0.324, p = 0.009; EQindex: r = 0.312, p = 0.011). Considering sex, relationship EQvas-6MWT decrease to small (r = 0.275; p = 0.028) whereas EQindex-6MWT remained moderated (r = 0.425; p = 0.000). When we considered women and men separately, the association between HRQoL and 6MWT appeared only in women, while the observed strong trend (p = 0.051) toward a large and positive association between EQindex and MIP, mediated by the covariate age, appeared only in men. Conversely to the cardiorespiratory fitness, MIP is not a limiting factor of HRQoL in healthy active elderly. Moreover, MIP and HRQoL should be included in the assessment of exercise interventions because they provide different information about the cardiorespiratory system deterioration. Similarly, EQvas and EQindex confirm to be complementary in the assessment of HRQoL. Furthermore, like aging process is different for men and women, the association between MIP and cardiorespiratory fitness with HRQoL may behave differently, so keeping on research these associations could help to improve training programs for this population

CSVS, a crowdsourcing database of the Spanish population genetic variability

The knowledge of the genetic variability of the local population is of utmost importance in personalized medicine and has been revealed as a critical factor for the discovery of new disease variants. Here, we present the Collaborative Spanish Variability Server (CSVS), which currently contains more than 2000 genomes and exomes of unrelated Spanish individuals. This database has been generated in a collaborative crowdsourcing effort collecting sequencing data produced by local genomic projects and for other purposes. Sequences have been grouped by ICD10 upper categories. A web interface allows querying the database removing one or more ICD10 categories. In this way, aggregated counts of allele frequencies of the pseudo-control Spanish population can be obtained for diseases belonging to the category removed. Interestingly, in addition to pseudo-control studies, some population studies can be made, as, for example, prevalence of pharmacogenomic variants, etc. In addition, this genomic data has been used to define the first Spanish Genome Reference Panel (SGRP1.0) for imputation. This is the first local repository of variability entirely produced by a crowdsourcing effort and constitutes an example for future initiatives to characterize local variability worldwide. CSVS is also part of the GA4GH Beacon network

Comprensión lectora en los estudiantes de bachillerato de la unidad educativa Uruguay de Portoviejo

Objective: To know the level of reading comprehension in high school students of the Uruguay Educational Unit in Portoviejo. Methods: The research is carried out under a mixed approach, with a sample of 76 students from the evening section belonging to the second year of parallel Baccalaureate “A” and “B”. To collect data, we worked with a survey based on a literary text provided to the students, which based on scales and correct answers determined the reading level at which they were; In addition, another survey was carried out that allowed us to know the interest, preference and time that students spent reading. Results: It was revealed that the majority of the students evaluated are at the literal level of reading comprehension, evidencing difficulties in making inferences and critical analysis, which is why it is necessary to implement innovative pedagogical strategies, take advantage of technological resources and strengthen the role of school libraries to promote a solid reading habit and improve reading comprehension levels in students. Conclusion: The fundamental role of reading in the learning process and the factors that influence interest in reading are emphasized, such as the use of technological devices, the family environment and the role of teachers as promoters of reading habits. Furthermore, the contribution of school libraries to the promotion of reading and the development of reading skills is highlighted.Objetivo: Conocer el nivel de comprensión lectora en los estudiantes de bachillerato de la Unidad Educativa Uruguay en Portoviejo. Métodos: La investigación se desarrolla bajo un enfoque mixto, con una muestra de 76 estudiantes de la sección vespertina pertenecientes a segundo de Bachillerato paralelo “A” y “B”. Para la recolección de datos se trabajó con una encuesta en base a un texto literario proporcionado a los estudiantes, que en base baremos y  repuestas correctas determinaba el nivel de lectura en el que se encontraban; además, se realizó otra encuesta que permitió conocer el interés, preferencia y tiempo que los estudiantes empleaban a la lectura. Resultados: Se reveló que la mayoría de los estudiantes evaluados se encuentran en el nivel literal de comprensión lectora, evidenciando dificultades para realizar inferencias y análisis crítico, por lo cual es necesario implementar estrategias pedagógicas innovadoras, aprovechar los recursos tecnológicos y fortalecer el rol de las bibliotecas escolares para promover un sólido hábito lector y mejorar los niveles de comprensión lectora en los estudiantes. Conclusión: Se enfatiza el rol fundamental de la lectura en el proceso de aprendizaje y los factores que influyen en el interés por la lectura, como el uso de dispositivos tecnológicos, el entorno familiar y el papel de los docentes como promotores de hábitos lectores. Además, se destaca la contribución de las bibliotecas escolares para el fomento de la lectura y el desarrollo de competencias lectoras

Metabolic Impairments Caused by Pesticides in Mammals and Their Interactions with Other Pollutants

The biological systems are exposed to a complex environment in which the contaminants can interact in a synergistic/antagonistic fashion and for this reason, the study of “chemical cocktails” is of great interest to fully understand the final biological effect. To evaluate the final biological response of a pollutant, it is essential to have an adequate analytical methodology that allows the correct monitoring of environmental systems in order to establish their quality, and, when appropriate, the application of corrective measures. Undoubtedly, massive methods “the omics” are among the most efficient current tools. To this end, transcriptomics, proteomics, metabolomics and chemical speciation can provide very useful information, mainly when they are combined. However, the combination of proteomics with metabolomics has some drawbacks as the temporal space is different (i.e. metabolomics gives information about what happens right now, but it can be related with numerous post-translational modifications happened previously). In this sense, it seems that the combination of genomics with metabolomics is easier. Thus, when metabolomics data are interpreted in combination with genomic, transcriptomic and proteomic results, in the so-called systems biology approach, a holistic knowledge of the organism/process under investigation can be achieved

Antioxidant Activity and Bio-Accessibility of Polyphenols in Black Carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) and Two Derived Products during Simulated Gastrointestinal Digestion and Colonic Fermentation

Black carrot has been attracting increasing thanks to its high bioactive compound content. This study presents the polyphenol bio-accessibility of black carrot and two derived products (black carrot snack (BC snack) and black carrot seasoning (BC seasoning)) after in vitro gastrointestinal digestion and colonic fermentation. Additionally, antioxidant activity was measured by 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays. Nine flavonoids and eight anthocyanins were determined by ultra high-performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) analysis, the predominant compounds being the hydroxycinnamic acids 3-O-feruloylquinic acid, 4-O-feruloylquinic acid and chlorogenic acid. The BC snack (108 µmol/g DW) presented the highest total polyphenol content, followed by BC seasoning (53 µmol/g DW) and black carrot (11.4 µmol/g DW). The main polyphenols still bio-accessible after in vitro digestion were the hydroxycinnamic acids, with mean recovery rates of 113 % for black carrot, 69% for BC snack and 81% for BC seasoning. The incubation of black carrot and its derived products with human faecal bacterial resulted in the complete degradation of anthocyanins and in the formation of mainly 3-(4′-hydroxyphenyl)propanoic acid as the major catabolic event. In conclusion, our results suggest that the black carrot matrix impacts significantly affects the bio-accessibility of polyphenols and, therefore, their potential health benefits

A multi-technique approach to characterization: the Sant Martí de Tous chert as a prehistoric resource for the NE of the Iberian Peninsula

The Sant Genis Formation is located in the NE of the Iberian Peninsula (Catalonia, Spain) and is dated to the Priabonian (upper Eocene), being part of the evaporitic formations of the margin of the Ebro Basin. It is formed by a succession of sandy lutites, occasional limestone layers, marls, and local stratified gypsum and cherts, including the Sant Marti de Tous chert. The Sant Marti de Tous chert type is confirmed by its abundance at specific locations within the territory (NE Iberian Peninsula). This is an important raw material procurement area, as evidenced by the presence of this chert in the main prehistoric sites of the region (e.g., Abric Romani) and the constant discovery of new sites in the area around the Sant Genis Formation, especially from the Neolithic period onwards (e.g., Cal Sitjo, La Guinardera Nord workshop). All these features, together with the great heterogeneity of the Cenozoic evaporitic cherts, prompted us to carry out a multi-proxy characterization for obtaining a valid criterion by which to identify this chert in the archaeological record. This very heterogeneity hampers the macroscopic characterization of archaeological cherts (e.g., at Abric Romani), but through petrographic analysis we have been able to identify their origins and, albeit to a lesser extent, their lithostratigraphic unit of provenance within the formation. The complementary mineralogical and geochemical techniques applied in this study show that, although it is difficult to establish an exact origin, through intensive sampling and criteria such as the differential presence of Fe and Ca, some differences among the siliceous varieties within a formation can be detected.CLT009/22/000044; PID2019-103987 GB-C3; PID2021-123092NB-C21; PID2019-105796 GB-I00; GV/2021/054; CEX2019-000945-Minfo:eu-repo/semantics/publishedVersio

Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003

Nitrogen (N) and phosphorus (P) deficiencies are two of the most agronomic problems that cause significant decrease in crop yield and quality. N and P chemical fertilizers are widely used in current agriculture, causing environmental problems and increasing production costs. Therefore, the development of alternative strategies to reduce the use of chemical fertilizers while maintaining N and P inputs are being investigated. Although dinitrogen is an abundant gas in the atmosphere, it requires biological nitrogen fixation (BNF) to be transformed into ammonium, a nitrogen source assimilable by living organisms. This process is bioenergetically expensive and, therefore, highly regulated. Factors like availability of other essential elements, as phosphorus, strongly influence BNF. However, the molecular mechanisms of these interactions are unclear. In this work, a physiological characterization of BNF and phosphorus mobilization (PM) from an insoluble form (Ca3(PO4)2) in Azotobacter chroococcum NCIMB 8003 was carried out. These processes were analyzed by quantitative proteomics in order to detect their molecular requirements and interactions. BNF led to a metabolic change beyond the proteins strictly necessary to carry out the process, including the metabolism related to other elements, like phosphorus. Also, changes in cell mobility, heme group synthesis and oxidative stress responses were observed. This study also revealed two phosphatases that seem to have the main role in PM, an exopolyphosphatase and a non-specific alkaline phosphatase PhoX. When both BNF and PM processes take place simultaneously, the synthesis of nitrogenous bases and L-methionine were also affected. Thus, although the interdependence is still unknown, possible biotechnological applications of these processes should take into account the indicated factors.Datos de investigación disponibles en: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1129721/full#supplementary-materia

The Nurse-Patient Relationship in Nursing Documentation: The Scope and Quality of Interactions and Prevalent Interventions in Inpatient Mental Health Units

Aims. (i) To evaluate the scope and quality of nurse-patient interactions recorded in the clinical notes of inpatient mental health units and (ii) to identify nursing interventions recorded in the context of the nurse-patient relationship in the clinical notes of inpatient mental health units. Design. A multimethod approach was use. Methods. Employing a quantitative cross-sectional design for the first aim, and a qualitative content analysis design of secondary data for the second aim. In total, 1,714 clinical notes were examined from 44 randomly selected patients who were hospitalized in five mental health units over the years 2022-2023. Results. The patient’s experience of the interaction was present in 69.9% (n = 1,198) of the notes. However, only 12.0% (n = 205) of the notes reached a sufficient standard of quality in terms of describing the nurse-patient interactions. Specifically, more than half of the notes did not reflect any type of nursing intervention (n = 723; 60.4%). Thirty interventions compatible with the nursing intervention classification were identified, of which more than 70% corresponded to domains in the physiological area. Conclusion. This study shows that the quantity and scope of patients’ clinical notes in mental health units do not sufficiently reflect the interventions performed by nurses, nor the quality or impact of these interventions in the context of the nurse-patient therapeutic relationship. Implications for the Profession and/or Patient Care. Improving the quality of clinical notes by integrating interventions and their impact can increase the quality of nursing care. Impact. The use of standardized nursing terminologies would contribute to the understanding of the extent and quality of nurse-patient interactions recorded in clinical notes. Thus, standardized documentation would also help to improve these interactions and their recording, which will facilitate decision-making. Reporting Method. Findings were reported using COREQ and STROBE guidelines. Patient or Public Contributions. There were no patient or public contributions.The authors are grateful for the support from the Department of Research and Universities of the Generalitat of Catalonia in the framework of the call for SGR-Cat 2021 grants, group 2021 SGR 1083