Fractured-Aquifer Hydrogeology from Geophysical Logs: Brunswick Group and Lockatong Formation, Pennsylvania

The Brunswick Group and the underlying Lockatong Formation are composed of lithified Mesozoic sediments that constitute part of the Newark Basin in southeastern Pennsylvania. These fractured rocks form an important regional aquifer that consists of gradational sequences of shale, siltstone, and sandstone, with fluid transport occurring primarily in fractures. An extensive suite of geophysical logs was obtained in seven wells located at the borough of Lansdale, Pennsylvania, in order to better characterize the areal hydrogeologic system and provide guidelines for the refinement of numerical ground water models. Six of the seven wells are approximately 120 m deep and the seventh extends to a depth of 335 m. Temperature, fluid conductivity, and flowmeter logs are used to locate zones of fluid exchange and to quantify transmissivities. Electrical resistivity and natural gamma logs together yield detailed stratigraphic information, and digital acoustic televiewer data provide magnetically oriented images of the borehole wall from which almost 900 fractures are identified

Developing conceptual hydrogeological model for Potsdam sandstones in southwestern Quebec, Canada

A hydrogeological study was conducted in Potsdam sandstones on the international border between Canada (Quebec) and the USA (New York). Two sandstone formations, arkose and conglomerate (base) and well-cemented quartz arenite (upper), underlie the study area and form the major regional aquifer unit. Glacial till, littoral sand and gravel, and marine silt and clay discontinuously overlie the aquifer. In both sandstone formations, sub-horizontal bedding planes are ubiquitous and display significant hydraulic conductivities that are orders of magnitude more permeable than the intact rock matrix. Aquifer tests demonstrate that the two formations have similar bulk hydrologic properties, with average hydraulic conductivities ranging from 2×10−5 to 4×10−5 m/s. However, due to their different lithologic and structural characteristics, these two sandstones impose rather different controls on groundwater flow patterns in the study area. Flow is sustained through two types of fracture networks: sub-horizontal, laterally extensive fractures in the basal sandstone, where hydraulic connectivity is very good horizontally but very poor vertically and each of the water-bearing bedding planes can be considered as a separate planar two-dimensional aquifer unit; and the more fractured and vertically jointed system found in the upper sandstone that promotes a more dispersed, three-dimensional movement of groundwater. Une étude hydrogéologique a été entreprise dans les grès de Potsdam, sur la frontière entre le Canada (Québec) et les Etats-Unis (New York). Sous le secteur d’étude, deux formations gréseuses, les arkoses et conglomérats (base) et les arénites quartzeuses cimentées (sommet), forment une unité aquifère majeure à l’échelle régionale. Les moraines glaciaires, les sables et graviers littoraux, et les argiles et silts marins recouvrent l’aquifère de manière discontinue. Dans les deux formations gréseuses, les litages sub-horizontaux sont omniprésents, et présentent des conductivités hydrauliques significatives, supérieures de plusieurs ordres de grandeur à celles de la matrice rocheuse intacte. Les pompages d’essai démontrent que les deux formations ont des propriétés hydrologiques apparentes comparables, avec notamment des conductivités hydrauliques comprises entre 2×10−5 et 4× 10−5 m/s. Cependant, du fait de leurs lithologies et de leurs caractéristiques structurales contrastées, ces deux formations gréseuses imposent des contrôles différents sur les écoulements souterrains dans le secteur d’étude. L’écoulement est soutenu par deux types de réseaux de fractures : des fractures latéralement extensives subhorizontales dans les grès de base, où la connectivité hydraulique est très bonne horizontalement mais médiocre verticalement, et où chacun des plans aquifères peut être considéré comme une unité aquifère isolée plane bidimensionnelle, et un système fissuré verticalement et plus fracturé situé dans les grès supérieurs, qui favorise des écoulements souterrains tridimensionnels et plus dispers

Fractured-Aquifer Hydrogeology from Geophysical Logs: Brunswick Group and Lockatong Formation, Pennsylvania

The Brunswick Group and the underlying Lockatong Formation are composed of lithified Mesozoic sediments that constitute part of the Newark Basin in southeastern Pennsylvania. These fractured rocks form an important regional aquifer that consists of gradational sequences of shale, siltstone, and sandstone, with fluid transport occurring primarily in fractures. An extensive suite of geophysical logs was obtained in seven wells located at the borough of Lansdale, Pennsylvania, in order to better characterize the areal hydrogeologic system and provide guidelines for the refinement of numerical ground water models. Six of the seven wells are approximately 120 m deep and the seventh extends to a depth of 335 m. Temperature, fluid conductivity, and flowmeter logs are used to locate zones of fluid exchange and to quantify transmissivities. Electrical resistivity and natural gamma logs together yield detailed stratigraphic information, and digital acoustic televiewer data provide magnetically oriented images of the borehole wall from which almost 900 fractures are identified

Tumour necrosis factor-α up-regulates macrophage migration inhibitory factor expression in endometrial stromal cells via the nuclear transcription factor NF-κB

BACKGROUND: A series of controlled changes including proliferation, secretion and menstrual shedding occur in the human endometrium during every normal menstrual cycle. Macrophage migration inhibitory factor (MIF), a multifunctional cytokine with numerous proinflammatory, immunomodulatory and angiogenic properties, appears to be expressed in the human endometrium and to follow a regulated cycle phase-dependent expression, but the mechanisms underlying endometrial MIF expression remain to be fully elucidated. METHODS AND RESULTS: Results from enzyme-linked immunosorbent assay (ELISA) demonstrated a significant dose- and time-dependent increase in MIF secretion by human endometrial cells in response to tumour necrosis factor-alpha (TNF-α) (0.1-100 ng/ml). This increase was also observed at the mRNA level as shown by reverse transcription (RT)-PCR. Curcumin (10−8 mol/l), a known nuclear factor (NF)-κB inhibitor, inhibited the TNF-α-induced pIκB phosphorylation as shown by western blotting, NF-κB translocation into the nucleus as shown by electrophoretic mobility shift assay, and MIF synthesis and secretion as measured by ELISA and RT-PCR. The expression of a dominant-negative NF-κB inhibitor (IκB) significantly decreased the TNF-α-induced MIF promoter activity as analysed by transient cell transfection. CONCLUSIONS: These results indicate clearly that TNF-α up-regulates the expression of MIF in endometrial stromal cells. This took place possibly through NF-κB activation, and may play an important role in the physiology of the human endometriu

Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia

Diaporthe (syn. Phomopsis) species are well-known saprobes, endophytes or pathogens on a range of plants. Several species have wide host ranges and multiple species may sometimes colonise the same host species. This study describes eight novel Diaporthe species isolated from live and/or dead tissue from the broad acre crops lupin, maize, mungbean, soybean and sunflower, and associated weed species in Queensland and New South Wales, as well as the environmental weed bitou bush (Chrysanthemoides monilifera subsp. rotundata) in eastern Australia. The new taxa are differentiated on the basis of morphology and DNA sequence analyses based on the nuclear ribosomal internal transcribed spacer region, and part of the translation elongation factor-1α and ß-tubulin genes. The possible agricultural significance of live weeds and crop residues ('green bridges') as well as dead weeds and crop residues ('brown bridges') in aiding survival of the newly described Diaporthe species is discussed

Costa Rica Rift hole deepened and logged

During Leg 111 of the Ocean Drilling Program, scientists on the drilling vessel JOIDES Resolution studied crustal structure and hydrothermal processes in the eastern equatorial Pacific. Leg 111 spent 43 days on its primary objective, deepening and logging Hole 5048, a deep reference hole in 5.9-million-year-old crust 200 km south of the spreading axis of the Costa Rica Rift. Even before Leg 111 , Hole 5048 was the deepest hole drilled into the oceanic crust, penetrating 274.5 m of sediments and 1,075.5 m of pillow lavas and sheeted dikes to a total depth of 1,350 m below sea floor (mbsf). Leg 111 deepened the hole by 212.3 m to a total depth of 1,562.3 mbsf (1,287.8 m into basement), and completed a highly successful suite of geophysical logs and experiments, including sampling of borehole waters

Running from Paris to Beijing: biomechanical and physiological consequences

Abstract The purpose of this study was to examine the physiological and biomechanical changes occurring in a subject after running 8,500 km in 161 days (i.e. 52.8 km daily). Three weeks before, 3 weeks after (POST) and 5 months after (POST?5) running from Paris to Beijing, energy cost of running (Cr), knee flexor and extensor isokinetic strength and biomechanical parameters (using a treadmill dynamometer) at different velocities were assessed in an experienced ultra-runner. At POST, there was a tendency toward a 'smoother' running pattern, as shown by (a) a higher stride frequency and duty factor, and a reduced aerial time without a change in contact time, (b) a lower maximal vertical force and loading rate at impact and (c) a decrease in both potential and kinetic energy changes at each step. This was associated with a detrimental effect on Cr (?6.2%) and a loss of strength at all angular velocities for both knee flexors and extensors. At POST?5, the subject returned to his original running patterns at low but not at high speeds and maximal strength remained reduced at low angular velocities (i.e. at high levels of force). It is suggested that the running pattern changes observed in the present study were a strategy adopted by the subject to reduce the deleterious effects of long distance running. However, the running pattern changes could partly be linked to the decrease in maximal strength

Distinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization.

The V3 loop of the HIV-1 envelope (Env) protein elicits a vigorous, but largely non-neutralizing antibody response directed to the V3-crown, whereas rare broadly neutralizing antibodies (bnAbs) target the V3-base. Challenging this view, we present V3-crown directed broadly neutralizing Designed Ankyrin Repeat Proteins (bnDs) matching the breadth of V3-base bnAbs. While most bnAbs target prefusion Env, V3-crown bnDs bind open Env conformations triggered by CD4 engagement. BnDs achieve breadth by focusing on highly conserved residues that are accessible in two distinct V3 conformations, one of which resembles CCR5-bound V3. We further show that these V3-crown conformations can, in principle, be attacked by antibodies. Supporting this conclusion, analysis of antibody binding activity in the Swiss 4.5 K HIV-1 cohort (n = 4,281) revealed a co-evolution of V3-crown reactivities and neutralization breadth. Our results indicate a role of V3-crown responses and its conformational preferences in bnAb development to be considered in preventive and therapeutic approaches