Rapid placement and anatomical sculpting of hardened posterior composite

Posterior composites have long had a reputation for being difficult and time consuming in clinical practice. In addition to this reputation, it has been noted that recurrent caries and in general failure of these restorations prematurely has been a problem. Relative to amalgams, posterior composites have vexed even good clinical dentist. This video presentation illustrates techniques that not only save time but also create more predictable high-quality restorations

Shear Bond Strength of Nine Dual Cured Build-Up Materials and a Light-Curing Adhesive System on Dentin

The purpose of this study was to measure the shear bond strength of nine different commercially available dual cured core build-up materials on human dentin in conjunction with a light curing adhesive system. The null hypothesis was that there is no statistical significant difference among the core build-up systems

Rapid placement and anatomical sculpting of hardened posterior composite

Posterior composites have long had a reputation for being difficult and time consuming in clinical practice. In addition to this reputation, it has been noted that recurrent caries and in general failure of these restorations prematurely has been a problem. Relative to amalgams, posterior composites have vexed even good clinical dentist. This video presentation illustrates techniques that not only save time but also create more predictable high-quality restorations

Shear Bond Strength of Nine Dual Cured Build-Up Materials and a Light-Curing Adhesive System on Dentin

The purpose of this study was to measure the shear bond strength of nine different commercially available dual cured core build-up materials on human dentin in conjunction with a light curing adhesive system. The null hypothesis was that there is no statistical significant difference among the core build-up systems

Diametral tensile strength and cost-effectiveness of commercially available fiber posts

The goal in this study was to evaluate the difference of diametral tensile strength(DTS) obtained from commercially available fiber posts for cementation in root canals. The price of each fiber post was then compared with the DTS to evaluate the cost-effectiveness. The null hypothesis was that there is no difference between the cost-effectiveness among the manufacturers

Diametral tensile strength and cost-effectiveness of commercially available fiber posts

The goal in this study was to evaluate the difference of diametral tensile strength(DTS) obtained from commercially available fiber posts for cementation in root canals. The price of each fiber post was then compared with the DTS to evaluate the cost-effectiveness. The null hypothesis was that there is no difference between the cost-effectiveness among the manufacturers

Loss of Mitochondrial Functions Associated with Azole Resistance in Candida glabrata Results in Enhanced Virulence in Mice▿†

Mitochondrial dysfunction is one of the possible mechanisms by which azole resistance can occur in Candida glabrata. Cells with mitochondrial DNA deficiency (so-called “petite mutants”) upregulate ATP binding cassette (ABC) transporter genes and thus display increased resistance to azoles. Isolation of such C. glabrata mutants from patients receiving antifungal therapy or prophylaxis has been rarely reported. In this study, we characterized two sequential and related C. glabrata isolates recovered from the same patient undergoing azole therapy. The first isolate (BPY40) was azole susceptible (fluconazole MIC, 4 μg/ml), and the second (BPY41) was azole resistant (fluconazole MIC, >256 μg/ml). BPY41 exhibited mitochondrial dysfunction and upregulation of the ABC transporter genes C. glabrata CDR1 (CgCDR1), CgCDR2, and CgSNQ2. We next assessed whether mitochondrial dysfunction conferred a selective advantage during host infection by testing the virulence of BPY40 and BPY41 in mice. Surprisingly, even with in vitro growth deficiency compared to BPY40, BPY41 was more virulent (as judged by mortality and fungal tissue burden) than BPY40 in both systemic and vaginal murine infection models. The increased virulence of the petite mutant correlated with a drastic gain of fitness in mice compared to that of its parental isolate. To understand this unexpected feature, genome-wide changes in gene expression driven by the petite mutation were analyzed by use of microarrays during in vitro growth. Enrichment of specific biological processes (oxido-reductive metabolism and the stress response) was observed in BPY41, all of which was consistent with mitochondrial dysfunction. Finally, some genes involved in cell wall remodelling were upregulated in BPY41 compared to BPY40, which may partially explain the enhanced virulence of BPY41. In conclusion, this study shows for the first time that mitochondrial dysfunction selected in vivo under azole therapy, even if strongly affecting in vitro growth characteristics, can confer a selective advantage under host conditions, allowing the C. glabrata mutant to be more virulent than wild-type isolates

Molecular Epidemiology of Tuberculosis in British Columbia, Canada: A 10-Year Retrospective Study

This 10-year retrospective study describes tuberculosis molecular epidemiology in a low-incidence setting. Approximately one-third of cases likely result from local transmission, largely – but not entirely – amongst the Canadian-born. Disease site and risk factors for clustering vary with lineage and birthplace

Chromosomal contacts connect loci associated with autism, BMI and head circumference phenotypes

Copy number variants (CNVs) are major contributors to genomic imbalance disorders. Phenotyping of 137 unrelated deletion and reciprocal duplication carriers of the distal 16p11.2 220 kb BP2-BP3 interval showed that these rearrangements are associated with autism spectrum disorders and mirror phenotypes of obesity/underweight and macrocephaly/microcephaly. Such phenotypes were previously associated with rearrangements of the non-overlapping proximal 16p11.2 600 kb BP4-BP5 interval. These two CNV-prone regions at 16p11.2 are reciprocally engaged in complex chromatin looping, as successfully confirmed by 4C-seq, fluorescence in situ hybridization and Hi-C, as well as coordinated expression and regulation of encompassed genes. We observed that genes differentially expressed in 16p11.2 BP4-BP5 CNV carriers are concomitantly modified in their chromatin interactions, suggesting that disruption of chromatin interplays could participate in the observed phenotypes. We also identified cis- and trans-acting chromatin contacts to other genomic regions previously associated with analogous phenotypes. For example, we uncovered that individuals with reciprocal rearrangements of the trans-contacted 2p15 locus similarly display mirror phenotypes on head circumference and weight. Our results indicate that chromosomal contacts’ maps could uncover functionally and clinically related genes