The use of molecular markers in conservation programmes of live animals

International audienc

A novel recycling route for spent li-ion batteries

In this work, a recycling route for spent Li-ion batteries (LIBs) was developed. For this, the recovery of the metal content in both electrodes (anode and cathode) was investigated. Based on these results, an economic analysis of this recycling process was carried out. The obtained results showed that more than 90% of the material contained in both electrodes was recycled. The dissolution with acetic acid of the metals present in the active cathodic material is thermodynamically viable and the addition of a reducing agent such as hydrogen peroxide improved the spontaneity of the reaction. Dissolutions close to 100% for Li and Co were obtained. In addition, it was determined that the synthesis of lithium and cobalt valuable compounds was viable from the leach liquor, recovering approximately 90% of Co as cobalt oxalate, and 92% of Li as lithium carbonate. Furthermore, carbon graphite and Cu were fully recovered (100%) from the anodes. Finally, the results of the economic analysis showed that the recovered products have a high commercial value and industrial interest, providing an environmentally and economically viable process.Fil: Pinna, Eliana Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; ArgentinaFil: Toro, Norman. Universidad Arturo Prat; ChileFil: Gallegos, Sandra. Universidad Arturo Prat; ChileFil: Rodriguez, Mario Humberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentin

Prilog proučavanju dvostrukih osobnih imena u seviljskim matičnim knjigama krštenih 16. stoljeća

En este artículo se estudian los nombres de pila dobles (por ejemplo, Ana María o Juan Francisco) extraídos de los libros de bautismo de Sevilla anteriores a 1600, investigación esbozada hace años por Menéndez Pidal y similar a la que han llevado a cabo, entre otros, García Gallarín y Castro en otras zonas de España. Del análisis efectuado a 1073 partidas bautismales se deduce tanto la influencia que ejerció la Iglesia católica en relación con la elección de estos nombres, como la inclinación que las clases altas de la sociedad tenían por los nombres dobles.U radu se analiziraju dvostruka osobna imena (npr. Ana María ili Juan Francisco) ekscerpirana iz šesnaestostoljetnih matičnih knjiga krštenih španjolskoga grada Seville. Autoru je kao poticaj i model poslužio rad Ramóna Menéndeza Pidala, a slična istraživanja u drugim španjolskim krajevima proveli su, među ostalima, García Gallarín i Castro. Analizirajući 1073 zapisa, autor proučava utjecaj Katoličke Crkve na odabir imena te utvrđuje imaju li pripadnici viših društvenih klasa posebnu sklonost nadijevanju dvostrukih imena

Convivencia escolar para el aprendizaje significativo en estudiantes del primer al tercer grado de la institución educativa 10783 – Lambayeque

La presente investigación, titulada Programa de convivencia escolar para mejorar el aprendizaje significativo en los estudiantes del primer al tercer grado de la institución educativa N° 10783 – Olmos Lambayeque, tuvo como objetivo principal proponer un programa de convivencia escolar, para mejorar el aprendizaje significativo en los estudiantes. El tipo de estudio fue descriptivo propositivo y su diseño no experimental, con una población de 64 niños y una muestra de 37. El instrumento utilizado fue un cuestionario, debidamente validado y aplicado a los niños de la muestra en donde se demostró que la mayoría de los estudiantes se ubican en el nivel medio, tanto en la variable aprendizaje significativo como en sus dimensiones. Ante esta situación se formuló el programa basado en convivencia escolar sustentado con la teoría de Vygotsky con capacidades y actividades que fueron diseñadas para mejorar la motivación, comprensión, funcionalidad, relación con la vida real y participación activa de los estudiantes, Una de las conclusiones fue que a través de la propuesta se elaboró el programa de convivencia escolar, para la mejora del aprendizaje significativo en los alumnos de la muestra, con el que podemos asegurar resultados positivos, tal como lo demuestran los logros alcanzados en los antecedentes

Visión poética de los paisajes de Canarias.

Este proyecto plantea una propuesta pictórica, estableciendo un vínculo entre la realidad del paisaje de Canarias y una mirada subjetiva sobre él. Al explorar el concepto de paisaje de una manera nostálgica y desoladora, se explora este género de la pintura como un escenario basado en nuestras propias suposiciones de lo que el paisaje puede significar bajo nuestra mirada.This project suggests a pictorial proposal, establishing a link between the reality of the Canary Islands landscape and a subjective look at it. By exploring the concept of landscape in a nostalgic and bleak way, this genre is investigated as a setting based on our own assumptions of what landscape means to us

Product complexity assessment for a Proactive-DFA implementation (Simplicity + Simplicity = Complexity)

This thesis presents product complexity as a criterion for the optimisation of product design in the light of an Assembly-Oriented Design and Design for Assembly implementation. It takes a holistic approach to the evaluation of the product architecture by presenting a set of indicators that help examine the product structure at two different levels: Assembly and Component complexity. Assembly complexity assessment is further sub-divided into Structural and Sequence complexity. The latter is a well-known and thoroughly studied area in assembly sequence evaluation, whereas the former gives a novel and original approach to drawing attention to those areas in the product configuration that will consume more resources (i.e. time and tooling required). Component complexity, on the other hand, is sub-divided into manufacturing and process handling/manipulation complexity. The first area has been addressed by the manufacturing analysis section of most Design for Assembly and Manufacturing methodologies, but it has been traditionally addressed as a manual and chart-based evaluation. This is a rigid approach that leaves little room for expansion and has no connection with the product structure. The metrics presented in this work embody a new approach that takes into account the component-to-component interactions and allows the analysis of component shape by extracting its geometry characteristics and comparing them with particular traits of the manufacturing processes available to the designer. Additionally, the metrics presented in this work can be used to make an assessment of the product complexity at a particular point (static complexity) in the development cycle. They can also be registered over a period of time to provide an estimate of the possible consequences of the decisions made during a part of the development cycle (dynamic complexity). By using the methods developed, designers could reduce production costs and increase the reliability of their products.Ph

Nucleotide sequence and endosperm-specific expression of the structural gene for the thionin alpha-hordothionin in barley (Hordeum vulgare L.)

A barley genomic library, obtained by cloning in the vector λEMBL-4, was screened with a cDNA probe encoding the α-hordothionin toxin. A positive clone, designated λTH1, was selected for further characterization. The coding and flanking regions of the α-hordothionin gene (Hth-1) were sequenced. Hth-1 has two introns of 420 and 91 nucleotides (nt), respectively. The promoter region has the following main features: one TATA box; three CATC boxes; an enhancer-like sequence, starting at nt position −282 from the first ATG codon, which is homologous to sequences appearing at similar positions in other endosperm genes; two versions of an 18-nt sequence that is more highly repeated in structural domains of several prolamin genes; two extensive regions close to the first ATG codon that are homologous to a sequence located much further upstream in the B-hordein promoter. The transcription start point was determined at nt positions −46 to −47, both by the S1 nuclease-protection and by the primer-extension assays. A maximum of 2–4 copies of the Hth-1 gene per haploid genome was determined by Southern-blot hybridization. Expression of the Hth-1 gene was detected during the cell proliferation stage of endosperm development (maximum at 13–16 days after pollinization) and was not detected in either etiolated or green coleoptile

Proteomic Analysis of HIV-Infected Macrophages

Mononuclear phagocytes (monocytes, macrophages, and microglia) play an important role in innate immunity against pathogens including HIV. These cells are also important viral reservoirs in the central nervous system and secrete inflammatory mediators and toxins that affect the tissue environment and function of surrounding cells. In the era of antiretroviral therapy, there are fewer of these inflammatory mediators. Proteomic approaches including surface enhancement laser desorption ionization, one- and two-dimensional difference in gel electrophoresis, and liquid chromatography tandem mass spectrometry have been used to uncover the proteins produced by in vitro HIV-infected monocytes, macrophages, and microglia. These approaches have advanced the understanding of novel mechanisms for HIV replication and neuronal damage. They have also been used in tissue macrophages that restrict HIV replication to understand the mechanisms of restriction for future therapies. In this review, we summarize the proteomic studies on HIV-infected mononuclear phagocytes and discuss other recent proteomic approaches that are starting to be applied to this field. As proteomic instruments and methods evolve to become more sensitive and quantitative, future studies are likely to identify more proteins that can be targeted for diagnosis or therapy and to uncover novel disease mechanisms

Neonatal exposure to xenoestrogens impairs the ovarian response to gonadotropin treatment in lambs

Bisphenol A (BPA) and diethylstilbestrol (DES) are xenoestrogens which have been associated with altered effects on reproduction. We hypothesized that neonatal xenoestrogen exposure affects the ovarian functionality in lambs. Thus, we evaluated the ovarian response to exogenous ovine Follicle Stimulating Hormone (oFSH) administered from postnatal day 30 (PND30) to PND32 in female lambs previously exposed to low doses of DES or BPA (BPA50: 50 μg/kg.day, BPA0.5: 0.5 μg/kg.day) from PND1 to PND14. We determined: a) follicular growth, b) circulating levels of E2, c) steroid receptors (ERA, ERB, AR) and atresia, d) mRNA expression levels of the ovarian bone morphogenetic protein (BMPs) system (BMP6, BMP15, BMP receptor type 1B, GDF9) and FSH receptor (FSHR). Lambs neonatally exposed to DES or BPA showed an impaired ovarian response to oFSH with a lower number of follicles ≥2 mm together with a lower number of atretic follicles and no increase in E2 serum levels in response to oFSH treatment. In addition, AR induction by oFSH was disrupted in granulosa and theca cells of lambs exposed to DES or BPA. An increase in GDF9 mRNA expression levels was observed in oFSH-primed lambs previously treated with DES or BPA50. In contrast, a decrease in BMPR1B was observed in BPA0.5-postnatally exposed lambs. The modifications in AR, GDF9 and BMPR1B may be associated with the altered ovarian function due to neonatal xenoestrogen exposure in response to an exogenous gonadotropin stimulus. These alterations may be the pathophysiological basis of subfertility syndrome in adulthood.Fil: Rivera, Oscar E.. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaFil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Rodriguez, Horacio Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Santamaría, Clarisa Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Bosquiazzo, Veronica Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Osti, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Belmonte, Norberto Miguel. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaFil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentin