3,618 research outputs found
Development of a novel equine influenza virus live-attenuated vaccine
H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a temperature sensitive (ts) H3N8 EIV live-attenuated influenza vaccine (LAIV) using reverse-genetics approaches. Our EIV LAIV was attenuated (att) in vivo and able to induce, upon a single intranasal administration, protection against H3N8 EIV wild-type (WT) challenge in both a mouse model and the natural host, the horse. Notably, since our EIV LAIV was generated using reverse genetics, the vaccine can be easily updated against drifting or emerging strains of EIV using the safety backbone of our EIV LAIV as master donor virus (MDV). These results demonstrate the feasibility of implementing a novel EIV LAIV approach for the prevention and control of currently circulating H3N8 EIVs in horse populations
VLA Observations of H I in the Helix Nebula (NGC 7293)
We report the detection of 21-cm line emission from H I in the planetary
nebula NGC 7293 (the Helix). The observations, made with the Very Large Array,
show the presence of a ring of atomic hydrogen that is associated with the
outer portion of the ionized nebula. This ring is most probably gas ejected in
the AGB phase that has been subsequently photodissociated by radiation from the
central star. The H I emission spreads over about 50 km/s in radial velocity.
The mass in H I is approximately 0.07 solar masses, about three times larger
than the mass in molecular hydrogen and comparable with the mass in ionized
hydrogen.Comment: 19 pages, 9 figure
Bioceramic nanocomposite thiol-acrylate polyHIPE scaffolds for enhanced osteoblastic cell culture in 3D
Emulsion-templated (polyHIPE) scaffolds for bone tissue engineering were produced by photopolymerisation of a mixture of trimethylolpropane tris(3-mercaptopropionate) and dipentaerythritol penta-/hexa-acrylate in the presence of hydroxyapatite (HA) or strontium-modified hydroxyapatite (SrHA) nanoparticles. Porous and permeable polyHIPE materials were produced regardless of the type or incorporation level of the bioceramic, although higher loadings resulted in a larger average pore diameter. Inclusion of HA and SrHA into the scaffolds was confirmed by EDX-SEM, FTIR and XPS and quantified by thermogravimetry. Addition of HA to polyHIPE scaffolds significantly enhanced compressive strength (148-216 kPa) without affecting compressive modulus (2.34-2.58 MPa). The resulting materials were evaluated in vitro as scaffolds for the 3D culture of MG63 osteoblastic cells vs. a commercial 3D cell culture scaffold (Alvetex®). Cells were able to migrate throughout all scaffolds, achieving a high density by the end of the culture period (21 days). The presence of HA and in particular SrHA gave greatly enhanced cell proliferation, as determined by staining of histological sections and total protein assay (Bradford). Furthermore, Von Kossa and Alizarin Red staining demonstrated significant mineralisation from inclusion of bioceramics, even at the earliest time point (day 7). Production of alkaline phosphatase (ALP), an early osteogenic marker, was used to investigate the influence of HA and SrHA on cell function. ALP levels were significantly reduced on HA- and SrHA-modified scaffolds by day 7, which agrees with the observed early onset of mineralisation in the presence of the bioceramics. The presented data support our conclusions that HA and SrHA enhance osteoblastic cell proliferation on polyHIPE scaffolds and promote early mineralisation
Oscillations and secondary bifurcations in nonlinear magnetoconvection
Complicated bifurcation structures that appear in nonlinear systems governed by partial differential equations (PDEs) can be explained by studying appropriate low-order amplitude equations. We demonstrate the power of this approach by considering compressible magnetoconvection. Numerical experiments reveal a transition from a regime with a subcritical Hopf bifurcation from the static solution, to one where finite-amplitude oscillations persist although there is no Hopf bifurcation from the static solution. This transition is associated with a codimension-two bifurcation with a pair of zero eigenvalues. We show that the bifurcation pattern found for the PDEs is indeed predicted by the second-order normal form equation (with cubic nonlinearities) for a Takens-Bogdanov bifurcation with Z2 symmetry. We then extend this equation by adding quintic nonlinearities and analyse the resulting system. Its predictions provide a qualitatively accurate description of solutions of the full PDEs over a wider range of parameter values. Replacing the reflecting (Z2) lateral boundary conditions with periodic [O(2)] boundaries allows stable travelling wave and modulated wave solutions to appear; they could be described by a third-order system
Monitoring the Large Proper Motions of Radio Sources in the Orion BN/KL Region
We present absolute astrometry of four radio sources in the
Becklin-Neugebauer/Kleinman-Low (BN/KL) region, derived from archival data
(taken in 1991, 1995, and 2000) as well as from new observations (taken in
2006). All data consist of 3.6 cm continuum emission and were taken with the
Very Large Array in its highest angular resolution A configuration. We confirm
the large proper motions of the BN object, the radio source I (GMR I) and the
radio counterpart of the infrared source n (Orion-n), with values from 15 to 26
km/s. The three sources are receding from a point between them from where they
seem to have been ejected about 500 years ago, probably via the disintegration
of a multiple stellar system. We present simulations of very compact stellar
groups that provide a plausible dynamical scenario for the observations. The
radio source Orion-n appeared as a double in the first three epochs, but as
single in 2006. We discuss this morphological change. The fourth source in the
region, GMR D, shows no statistically significant proper motions. We also
present new, accurate relative astrometry between BN and radio source I that
restrict possible dynamical scenarios for the region. During the 2006
observations, the radio source GMR A, located about 1' to the NW of the BN/KL
region, exhibited an increase in its flux density of a factor of ~3.5 over a
timescale of one hour. This rapid variability at cm wavelengths is similar to
that previously found during a flare at millimeter wavelengths that took place
in 2003.Comment: Accepted for publication in Ap
The constant-velocity highly collimated outflows of the planetary nebula He 2-90
We present high-dispersion echelle spectroscopic observations and a
narrow-band [N II] image of the remarkable jet-like features of He 2-90. They
are detected in the echelle spectra in the H-alpha and [N II] lines but not in
other nebular lines. The [N II]/H-alpha ratio is uniformly high, ~1. The
observed kinematics reveals bipolar collimated outflows in the jet-like
features and shows that the southeast (northwest) component expands towards
(away from) the observer at a remarkably constant line-of-sight velocity,
26.0+-0.5 km/s. The observed expansion velocity and the opening angle of the
jet-like features are used to estimate an inclination angle of ~5 degrees with
respect to the sky plane and a space expansion velocity of ~290 km/s. The
spectrum of the bright central nebula reveals a profusion of Fe lines and
extended wings of the H-alpha line, similar to those seen in symbiotic stars
and some young planetary nebulae that are presumed to host a mass-exchanging
binary system. If this is the case for He 2-90, the constant velocity and
direction of the jets require a very stable dynamic system against precession
and warping.Comment: 8 pages (emulate ApJ), 5 figure, 1 tabl
Genomes-based phylogeny of the genus Xanthomonas
<p>Abstract</p> <p>Background</p> <p>The genus <it>Xanthomonas </it>comprises several plant pathogenic bacteria affecting a wide range of hosts. Despite the economic, industrial and biological importance of <it>Xanthomonas</it>, the classification and phylogenetic relationships within the genus are still under active debate. Some of the relationships between pathovars and species have not been thoroughly clarified, with old pathovars becoming new species. A change in the genus name has been recently suggested for <it>Xanthomonas albilineans</it>, an early branching species currently located in this genus, but a thorough phylogenomic reconstruction would aid in solving these and other discrepancies in this genus.</p> <p>Results</p> <p>Here we report the results of the genome-wide analysis of DNA sequences from 989 orthologous groups from 17 <it>Xanthomonas </it>spp. genomes available to date, representing all major lineages within the genus. The phylogenetic and computational analyses used in this study have been automated in a Perl package designated Unus, which provides a framework for phylogenomic analyses which can be applied to other datasets at the genomic level. Unus can also be easily incorporated into other phylogenomic pipelines.</p> <p>Conclusions</p> <p>Our phylogeny agrees with previous phylogenetic topologies on the genus, but revealed that the genomes of <it>Xanthomonas citri </it>and <it>Xanthomonas fuscans </it>belong to the same species, and that of <it>Xanthomonas albilineans </it>is basal to the joint clade of <it>Xanthomonas </it>and <it>Xylella fastidiosa</it>. Genome reduction was identified in the species <it>Xanthomonas vasicola </it>in addition to the previously identified reduction in <it>Xanthomonas albilineans</it>. Lateral gene transfer was also observed in two gene clusters.</p
Imaging the Inner and Outer Gaps of the Pre-Transitional Disk of HD 169142 at 7 mm
We present Very Large Array observations at 7 mm that trace the thermal
emission of large dust grains in the HD 169142 protoplanetary disk. Our images
show a ring of enhanced emission of radius ~25-30 AU, whose inner region is
devoid of detectable 7 mm emission. We interpret this ring as tracing the rim
of an inner cavity or gap, possibly created by a planet or a substellar
companion. The ring appears asymmetric, with the western part significantly
brighter than the eastern one. This azimuthal asymmetry is reminiscent of the
lopsided structures that are expected to be produced as a consequence of
trapping of large dust grains. Our observations also reveal an outer annular
gap at radii from ~40 to ~70 AU. Unlike other sources, the radii of the inner
cavity, the ring, and the outer gap observed in the 7 mm images, which trace
preferentially the distribution of large (mm/cm sized) dust grains, coincide
with those obtained from a previous near-infrared polarimetric image, which
traces scattered light from small (micron- sized) dust grains. We model the
broad-band spectral energy distribution and the 7 mm images to constrain the
disk physical structure. From this modeling we infer the presence of a small
(radius ~0.6 AU) residual disk inside the central cavity, indicating that the
HD 169142 disk is a pre-transitional disk. The distribution of dust in three
annuli with gaps in between them suggests that the disk in HD 169142 is being
disrupted by at least two planets or substellar objects.Comment: Accepted by ApJ Letters, 16 pages, 3 figures, ApJ Letters 201
Naming the unnamed: over 65,000 Candidatus names for unnamed Archaea and Bacteria in the Genome Taxonomy Database
Thousands of new bacterial and archaeal species and higher-level taxa are discovered each year through the analysis of genomes and metagenomes. The Genome Taxonomy Database (GTDB) provides hierarchical sequence-based descriptions and classifications for new and as-yet-unnamed taxa. However, bacterial nomenclature, as currently configured, cannot keep up with the need for new well-formed names. Instead, microbiologists have been forced to use hard-to-remember alphanumeric placeholder labels. Here, we exploit an approach to the generation of well-formed arbitrary Latinate names at a scale sufficient to name tens of thousands of unnamed taxa within GTDB. These newly created names represent an important resource for the microbiology community, facilitating communication between bioinformaticians, microbiologists and taxonomists, while populating the emerging landscape of microbial taxonomic and functional discovery with accessible and memorable linguistic labels
Compact Radio Sources in Orion: New Detections, Time Variability, and Objects in OMC-1S
We present the analysis of four 3.6 cm radio continuum archival observations
of Orion obtained using the Very Large Array in its A-configuration, with
angular resolution. The observations were made during the period
1994-1997. In a region of , we detect a total of 77 compact radio
sources. Of the total of detected sources, 54 are detected in one or more of
the individual observations and 36 of these show time variability (by more than
30%) between the observed epochs. A deep image made from averaging all data
shows an additional 23 faint sources, in the range of 0.1 to 0.3 mJy. Of the
total of 77 sources, 39 are new centimeter detections. However, only 9 of the
77 sources do not have a previously reported counterpart at near-infrared,
optical, or X-ray wavelengths. In particular, we detect three faint sources in
the OMC-1S region that may be related to the sources that power the multiple
outflows that emanate from this part of the Orion nebula. %We discuss the
nature of these sources and its relation with the %near-infrared, optical, and
X-ray objects in the region.Comment: 29 pages, 9 figure
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