Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Alcohol Consumption in the Colombian Population, 2015 National Mental Health Survey

Introducción La Encuesta Nacional de Salud Mental (ENSM) de 2015 indagó en Colombia, entre otros asuntos, sobre el de consumo de alcohol de las personas entre 12 y 17 años y de 18 y más años. Se presentaron los datos según edad y región, sin realizar cruces con otras variables de salud mental, problemas, trastornos, acceso a servicios, estados de salud y su valoración. Objetivo Evaluar la prevalencia del consumo de alcohol en la población colombiana de edad ≥ 12 años teniendo en cuenta las variables sociodemográficas y el cribado clínico. Métodos Análisis secundario de base de datos de la ENSM 2015; constituyeron la muestra 15.231 personas de 12 a 96 años de 13.200 hogares de cinco regiones del país (Atlántica, Bogotá, Central, Oriental y Pacífica). Se usó el Alcohol and Use Disorder Identification Test, corto y completo (AUDIT y AUDIT-C), con análisis estratificado según puntuación y otras variables incluidas en la encuesta. Resultados El 2,7% de los menores estudiados entran en la categoría de bebedor de riesgo. El mayor porcentaje de bebedores de riesgo se ubica en la franja de 18 a 44 años, con clara mayoría de varones. Conclusiones El estudio encuentra que un AUDIT-C positivo en mayores de edad se asocia con mayor porcentaje de no presentar síntomas ansiedad y menos problemas de ansiedad, eventos traumáticos y eventos traumáticos por conflicto armado reportados. Esto requiere más estudios. Las personas adultas con AUDIT-C positivo tienen mayor percepción de bienestar, aunque también se halla mayor porcentaje de hogares en estado de pobreza. El estudio de factores individuales, sociales, familiares y ambientales en poblaciones específicas se debe desarrollar para realizar intervenciones más adecuadas.Q476-88Introduction National Survey of Mental Health (ENSM) in 2015 in Colombia asks, among other conditions, about alcohol consumption in people 12-17 years old, and 18 and older. Data were presented by age and region, with no cross-references to other categories of mental health problems, disorders, access to services, and health status. Objective To assess alcohol consumption in Colombia, taking into account sociodemographic and clinical screening categories included. Methods Secondary database analysis, sample size: 15,231 people from 13,200 households of five regions (Atlantic, Bogotá, Central, Eastern, and Pacific), with an age range from 12 to 96 years. AUDIT and AUDIT-C were used and stratified according to score and other variables included in the survey analysis. Results The high-risk drinking category was observed in 2.7% of children studied, with the highest percentage of drinking risk lying in the range of 18 to 44 years, with a clear majority of men. Conclusions The study finds that a positive AUDIT-C in adults is associated with a higher percentage of non-anxiety, less anxiety problems, and traumatic events and traumatic events related to armed conflict. This requires further studies. Adults with positive AUDIT-C have a greater perception of well-being, but also a higher percentage of households in poverty. The study of individual, social, family and environmental factors in specific populations should be developed in order to make more appropriate interventions

Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría

Immunophenotypic analysis of normal cell samples from bone marrow: applications in quality control of cytometry laboratories.Objective. To describe a standardized flow cytometry protocol for the relative and absolute quantification of hematopoietic cell subpopulationsfrom normal bone marrow, and to evaluate the expression of different lineage-specific cell markers with a reactivity associated to celldifferentiation to be used as part of the routine quality control in cytometry laboratories. Materials and methods. The immunophenotypicalanalysis of different cell subpopulations was done with samples from normal bone marrow using a panel of monoclonal and polyclonalantibodies useful in the characterization of acute leukemias with four different fluorescences, by means of a protocol that combines celllabeling of membrane and cytoplasm antigens. Expression analysis was done in terms of mean fluorescence intensity (MFI). Fluorescentbeads at a known concentration were added for calculating the absolute count of cells. Results. The antibody panel used allowed theidentification and quantification of different normal leukocyte subpopulations of lymphatic and myeloid origin, including CD34+ stem cells and more differentiated cell populations in the granulocytic, monocytic, and erythroid cell lines. We established reference values for cellpopulations and cell marker expression ranges as part of routine quality control of cytometry laboratories. Conclusion. Immunophenotypicpatterns identified as well as absolute and relative reference values for the different normal leukocyte populations from bone marrow can beused by cytometry laboratories as a basis for establishing reference parameters in phenotypic analyses of hematologic neoplasia

An International Society for Cell and Gene Therapy Mesenchymal Stromal Cells (MSC) Committee perspectives on International Standards Organization/Technical Committee 276 Biobanking Standards for bone marrow-MSCs and umbilical cord tissue-derived MSCs for research purposes

International audienceThe rapidly growing field of mesenchymal stromal cell (MSC) basic and translational research requires standardization of terminology and functional characterization. The International Standards Organization’s (ISO) Technical Committee (TC) on Biotechnology, working with extensive input from the International Society for Cells and Gene Therapy (ISCT), has recently published ISO standardization documents that are focused on biobanking of MSCs from two tissue sources, Wharton’s Jelly, MSC(WJ) and Bone Marrow, MSC(M)), for research and development purposes and development. This manuscript explains the path towards the consensus on the following two documents: the Technical Standard ISO/TS 22859 for MSC(WJ) and the full ISO Standard 24651 for MSC(M) biobanking. The ISO standardization documents are aligned with ISCT’s MSC committee position and recommendations on nomenclature because there was active input and incorporation of ISCT MSC committee recommendations in the development of these standards. The ISO standardization documents contain both requirements and recommendations for functional characterization of MSC(WJ) and MSC(M) using a matrix of assays. Importantly, the ISO standardization documents have a carefully defined scope and are meant for research use of culture expanded MSC(WJ) and MSC(M). The ISO standardization documents can be updated in a revision process and will be systematically reviewed after 3-5 years as scientific insights grow. They represent international consensus on MSC identity, definition, and characterization; are rigorous in detailing multivariate characterization of MSCs and represent an evolving-but-important first step in standardization of MSC biobanking and characterization for research use and development

Revista Temas Agrarios Volumen 26; Suplemento 1 de 2021

1st International and 2nd National Symposium of Agronomic Sciences: The rebirth of the scientific discussion space for the Colombian Agro.1 Simposio Intenacional y 2 Nacional de Ciencias Agronómicas: El renacer del espacio de discusión científica para el Agro colombiano

NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data