Effect of dance practice during pregnancy on mother´s health and fetus´s health. A systematic review

Objetivo: Realizar una revisión sistemática con el objetivo de determinar los efectos y los posibles beneficios de la práctica de la danza durante el embarazo, sobre la salud de la madre y del feto. Metodología: Se llevó a cabo una revisión sistemática (n=13) en las bases de datos de Web of Science, SportDiscus, Google Académico y Embase utilizando las palabras claves: «dance» (danza), «pregnancy»(embarazo), «perinatal» (perinatal) y «fetus»(feto), en inglés y en español, hasta septiembre de 2021. Previamente se elaboró una hoja de codificación basada en el objetivo del estudio y en la declaración PRISMA. Resultados: 1) La danza aeróbica combinada con el trabajo de otros componentes de la condición física puede prevenir el aumento excesivo de peso durante la gestación, reducir el riesgo de hipertensión, reducir el riesgo de padecer diabetes gestacional y mejorar la capacidad cardiorrespiratoria submáxima en mujeres gestantes; 2) Un programa únicamente de danza aeróbica podría prevenir y mejorar el dolor lumbopélvico común durante el embarazo; 3) No se observa evidencia de daño sobre la salud de la madre o del feto como consecuencia de la práctica de la danza; y 4) Se observa una evidencia limitada de los efectos positivos en la salud del feto y del neonato. Conclusiones: La práctica de la danza en mujeres gestantes podría contrarrestar algunos de los trastornos susceptibles propios de la etapa de gestación y no supone ningún riesgo para su salud. Sin embargo, son necesarios más estudios sobre los efectos de su práctica sobre la salud fetal. Así como, intervenciones considerando otros estilos de danza posibles

Development and evaluation of a nanofiber membrane in vitro as a therapeutic alternative for the post treatment in breast cancer cell in a murine model

Worldwide female breast cancer is the most commonly diagnosed cancer, with an estimated 2.3 million new cases in 2020, reason for the need of targeted therapies that can maximize treatment success and minimize toxicity. Nanoparticles of gold (AuNps) exhibit cytotoxic properties against certain types of cancer cell lines. Nanofibers have been use in the drug delivery systems due to its degradability and high surface area. We proposed a membrane with nanometric fibers using polivinilic alcohol (PVA) and chitosan (Qts) loaded with AuNps and Doxorubicin (Doxo) with the purpose of diminish tumor regression. PVA-Qts membrane was develop with electrospinning, the injection, voltage, distance and relative humidity parameters were standardized and it were characterized by Microscopic Atomic Force. The cytotoxicity with a median lethal dose (DL50) in two cell lines, breast adenocarcinoma murine (4T1) and murine fibroblast (NiH3T3) as a healthy control were evaluated. AuNps had a size of 3 nanometers (nm) with a Z potential of 13.2 mVolts and a DL50 of 75 µM in the cell line 4T1. Doxo was decrease in 95% with a final concentration of 0.03 mg/cm2. Both doses were loaded in the PVA-Qts solution. PVA-Qts-Doxo and PVA-Qts-AuNps-Doxo decrease the viability in 4T1 in 24 hours with a 15%, 72 hours with a 28%, the first with 60% and the latter with 82%. PVA-Qts-Doxo and PVA-Qts-AuNps-Doxo in NiH3T3 diminish incrementing with the time reaching a 40% in 120 hours. Finally, The viability for 4T1 cultured on PVA-Qts-Doxo was minor than in NiH3T3. The amount of Doxo in the membrane synthetized was 95% less than the employ doses, demonstrating that the fiber improves the delivery of the chemotherapeutic in a palatine time

Necesidades de orientación para el empleo en Andalucía

En nuestro país se constata la escasez de estudios que recojan análisis sobre las necesidades de orientación de personas adultas. Si bien disponemos de cierta información sobre las necesidades de grupos específicos (universitarios, mujeres, inmigrantes…), carecemos de un marco común que caracterice a la población adulta en su conjunto. Esta comunicación presenta los resultados de un estudio cuyo objetivo es identificar las necesidades de orientación para la elaboración del proyecto vital/profesional de las personas adultas, desarrollado en la Comunidad Autónoma Andaluza. En concreto, se ha aplicado un cuestionario, elaborado por el equipo de investigación, a una muestra de 501 personas usuarias de los servicios de orientación para el empleo. Los análisis realizados adelantan que los/as usuarios declaran tener mayor nivel de necesidad en el ámbito de las habilidades de autoexploración, seguido de las habilidades para la gestión y puesta en práctica del proyecto vital/profesionalIn our country, there is a lack of studies based on the needs of guidance analysis of adult people. We have some information about the needs of guidance of specific groups (undergraduates, women, immigrants,...). Nevertheless, we do not have a common framework that characterizes the adult population as a whole. This paper presents the results of a study aimed at identifying the needs of guidance to development the professional and vital project of adult people. This study has been developed at the Autonomous Community of Andalusia. Specifically, we applied a questionnaire, created by the research team, to 501 users of guidance services for employment. The analysis carried out put forward that the users have higher levels of need in the field of self-examination skills, followed by management and implementation skills of vital/professional project

Oral administration of pH-responsive polymeric nanoparticles based on zein and their therapeutic potential on cancer.

Background: Zein is a water-insoluble protein extracted from the endosperm of corn seeds, this polymer is an attractive matrix to encapsulate hydrophilic compounds because of its high proportion of hydrophobic amino acids, making it a potential smart delivery material for several treatments in the biopharmaceutical industry. nanoparticles have been used as drug delivery systems for the improvement of oral bioavailability; however, the strategies of nanoparticle obtention need the addition of stabilizers. in this study, a modified method to obtain zein nanoparticles was developed. Methods: Zein nanoparticles (ZNps) were made by a thermal treatment and precipitated into ovalbumin at a ratio of 1:3 on constant revolution. They were characterized by DLS, z potential, SEM, FT-IR. The encapsulation efficiency and release profile was measured following the bicinchoninic acid protocol and the viability assay was evaluated used HT29 (Human colon cancer cell line) and Huvec (Human umbilical vein endothelial cells). Results: Thermic treatment on zein allowed the obtention of ph-dependant nanoparticles without additional stabilizer, using the antisolvent precipitation method. additionally, the encapsulation efficiency was improved at 20% in comparison to other syntheses. the release profile on a gastrointestinal in vitro model of zein nanoparticles showed their capability as an oral drug delivery system; for this the egg white protein, ovabulmin, was used as a charged model. Finally, the cytotoxic effect of zein nanoparticles and zein solutions against cell lines were evaluated. zein nanoparticles highly decreased the viability on HT29 colon cancer cells in comparison to HUVEC endothelial cells. Conclusion: In this study, the synthesis of zein nanoparticles was optimized without the use of additives or stabilizers by the implementation of heat treatment. The average size of ZNps was 125 to 150 nm, these differences might be the result of the increment of protonation levels and intermolecular forces during antisolvent precipitation when a soluble protein is encapsulated. Both groups showed stability for 30 days. The wavelength splits shown on FT-IR analysis could indicate a rearrangement from tertiary to secondary structure. Encapsulation efficiency was at 70%, improving other methodologies by 20%. The release profile on a gastrointestinal in vitro model demonstrates that zein nanoparticles reach a maximum point at 240 min in acid conditions a 120 min in a basic environment, similar to an intestinal scenario providing enough time for gastric emptying. Pure zein affects the cell viability in a minor proportion compared to a nanoparticle form in both cell lines evaluated; ZNps showed a major cytotoxicity percentage in HT29 than in Huvec, which suggests a potential therapeutic effect as a drug delivery vehicle against cancer. Finally, this system has the potential to be developed as a novel strategy for an alternative to oral drug delivery and should be further studied on a wide variety of treatments

Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection

Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and delaying the emergence of resistant virus. The paramyxoviridae family includes pathogens causing morbidity and mortality worldwide in humans and animals, such as the Newcastle Disease Virus (NDV) in poultry. This study aims at determining the antiviral activity and mechanism of action in vitro of an ulvan (SP from the green seaweed Ulva clathrata), and of its mixture with a fucoidan (SP from Cladosiphon okamuranus), against La Sota NDV strain. The ulvan antiviral activity was tested using syncytia formation, exhibiting an IC50 of 0.1 μg/mL; ulvan had a better anti cell-cell spread effect than that previously shown for fucoidan, and inhibited cell-cell fusion via a direct effect on the F0 protein, but did not show any virucidal effect. The mixture of ulvan and fucoidan showed a greater anti-spread effect than SPs alone, but ulvan antagonizes the effect of fucoidan on the viral attachment/entry. Both SPs may be promising antivirals against paramyxovirus infection but their mixture has no clear synergistic advantag

shRNA targeting caspase-3 inhibits apoptosis and cell detachment induced by Pemphigus Vulgaris autoantibodies

Pemphigus is an organ-specific autoimmune disease that affects the skin and mucous membranes. It is induced by the deposition of pemphigus IgG autoantibodies, which mainly target Dsg1 and 3 and cause a loss of cell adhesion in a phenomenon known as acantholysis, and clinically is reflected as intraepidermal blistering. The present work assessed the effect of pemphigus vulgaris IgG (PV-IgG) on cell adhesion and caspase 3- dependent apoptosis in HaCaT cells. The expression of caspase-3 induced by PV-IgG was silenced in cells pre-treated with caspase 3-shRNA. PV-IgG induced cell detachment and apoptotic changes as demonstrated by the annexin-FITC assays. Treatment of cell cultures with normal IgG (control; N-IgG) did not have relevant effects on the aforementioned parameters. Then, the effect of PV-IgG on cells previously treated with shRNA was tested. The results demonstrated that shRNA reduced apoptotic features and the relative expression of caspase-3 measured by qRT-PCR, which showed a decrease of 96%. In conclusion shRNA prevented cell detachment and apoptosis of HaCaT cells induced by PV-IgG. The presented results further our understanding of the molecular pathophysiologic mechanisms involved in pemphigus diseases.Pemphigus is an organ-specific autoimmune disease that affects the skin and mucous membranes. It is induced by the deposition of pemphigus IgG autoantibodies, which mainly target Dsg1 and 3 and cause a loss of cell adhesion in a phenomenon known as acantholysis, and clinically is reflected as intraepidermal blistering. The present work assessed the effect of pemphigus vulgaris IgG (PV-IgG) on cell adhesion and caspase 3- dependent apoptosis in HaCaT cells. The expression of caspase-3 induced by PV-IgG was silenced in cells pre-treated with caspase 3-shRNA. PV-IgG induced cell detachment and apoptotic changes as demonstrated by the annexin-FITC assays. Treatment of cell cultures with normal IgG (control; N-IgG) did not have relevant effects on the aforementioned parameters. Then, the effect of PV-IgG on cells previously treated with shRNA was tested. The results demonstrated that shRNA reduced apoptotic features and the relative expression of caspase-3 measured by qRT-PCR, which showed a decrease of 96%. In conclusion shRNA prevented cell detachment and apoptosis of HaCaT cells induced by PV-IgG. The presented results further our understanding of the molecular pathophysiologic mechanisms involved in pemphigus diseases

Andalusian initial vocational training students: some contributions on their guidance's needs

El artículo presenta los principales resultados obtenidos con la técnica proyectiva-mediadora del fotolenguaje en una investigación2 sobre la orientación del alumnado de Formación Profesional Inicial (FP) en el sistema educativo español (tanto en Programas de Cualificación Profesional Inicial como Ciclos Formativos de Grado Medio y Superior). Se conformaron 9 grupos de estudiantes de FP escolarizados en centros de la provincia de Sevilla con el objetivo de obtener datos discursivos relacionados con sus necesidades de orientación. El análisis de los datos obtenidos ha permitido describir las trayectorias escolares de los estudiantes de los tres niveles de FP en el pasado, la percepción que tienen de su situación actual y sus aspiraciones de cara al futuro. Se han detectado algunas características comunes a los tres niveles como la experiencia escolar problemática, el deseo de continuar la formación y la incertidumbre respecto a los proyectos futuros. La información recogida ha posibilitado establecer dos ámbitos de orientación preferentes para este alumnado: la orientación personal dirigida a recuperar el autoconcepto y la confianza en las propias posibilidades de éxito; y la orientación destinada a ayudarle a construir su proyecto profesional y vital y a elegir adecuadamente la formación necesaria para ello.This paper presents some findings from data collection, by means of Photolanguage technique, which has been conducted among 9 vocational students’ groups located inside vocational institutes of the county of Seville. Data analysis allowed us to outline schooling paths’ features of the three levels of vocational pupils, the actual perception about their present training situation as well as their future Life-study-and-employment expectancies. It was possible to establish some common trends among students, as for example, the problematic schooling experience in the past, the present and widespread wish of continuing vocational training studies, and the uncertainty on the possibilities of personal project’s success. These and others outcomes pointed out to two main guidance delivery actions: the first one might deal with the students’ need to recover positive self-concept, as well as confidence on the possibilities of their own success; the second one is related to support vocational students at their attempts to build up a life-and professional project, and to take a suitable study choice

Bioactive Products From Plant-Endophytic Gram-Positive Bacteria

Endophytes constitute plant-colonizing microorganisms in a mutualistic symbiosis relationship. They are found in most ecosystems reducing plant crops’ biotic and abiotic stressors by stimulating immune responses, excluding plant pathogens by niche competition, and participating in antioxidant activities and phenylpropanoid metabolism, whose activation produces plant defense, structural support, and survival molecules. In fact, metabolomic studies have demonstrated that endophyte genes associated to specific metabolites are involved in plant growth promotion (PGP) by stimulating plant hormones production such as auxins and gibberellins or as plant protective agents against microbial pathogens, cancer, and insect pests, but eco-friendly and eco-safe. A number of metabolites of Gram-positive endophytes isolated from agriculture, forest, mangrove, and medicinal plants, mainly related to the Firmicutes phyla, possess distinctive biocontrol and plant growth-promoting activities. In general, Actinobacteria and Bacillus endophytes produce aromatic compounds, lipopeptides, plant hormones, polysaccharides, and several enzymes linked to phenylpropanoid metabolism, thus representing high potential for PGP and crop management strategies. Furthermore, Actinobacteria have been shown to produce metabolites with antimicrobial and antitumor activities, useful in agriculture, medicine, and veterinary areas. The great endophytes diversity, their metabolites production, and their adaptation to stress conditions make them a suitable and unlimited source of novel metabolites, whose application could reduce agrochemicals usage in food and drugs production

The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth

Abstract. Immunogenic cell death is a cell death modality that stimulates the immune system to combat cancer cells. IMMUNEPOTENT CRP (ICRP) is a mixture of substances of low molecular weight obtained from bovine spleens that exhibits in vitro cytotoxic activity on different tumor cell lines and modulates the immune response in vivo. The aim of the present study was to determine whether the cytotoxic effect of ICRP and its combination with oxaliplatin (OXP) on murine melanoma B16F10 cells was due to immunogenic cell death. The cytotoxic assay was performed using flow cytometry to detect Annexin V and propidium iodide staining, and calreticulin (CRT) exposure. Adenosine triphosphate, heat shock protein (HSP) 70, HSP90 and high mobility group box 1 (HMGB1) release were identified using bioluminescence, western blot and ELISA assays, respectively. The present in vitro study demonstrated that treatments with ICRP or OXP induced cell death in a time-dependent manner, but treatment with the combination of ICRP + OXP increased the cytotoxic effect following 24 h of treatment. CRT exposure and release of adenosine triphosphate (ATP), HSP70, HSP90 and HMGB1 were induced by treatment with ICRP, and the combination of ICRP + OXP increased the exposure and release of damage-associated molecular patterns (DAMPs), while OXP treatment only induced CRT exposure, ATP and HMGB1 release. The in vivo experiments demonstrated that administration of tumor-derived DAMP-rich cell lysates derived from B16F10 cells treated with ICRP and the combination of ICRP + OXP prevented melanoma growth; however, OXP treatment did not. These results suggested that IMMUNEPOTENT CRP may be used as an agent to increase the ability of antitumor drugs to induce immunogenic cell death and prevent the growth of melanoma

Increased efficacy and extended shelf life of spinosad formulated in phagostimulant granules against Spodoptera frugiperda

Abstract BACKGROUND:SpinosadisrecommendedforcontrolofSpodopterafrugiperda(J.E.Smith)larvae;itsapplicationwithphagostimulants may reduce the quantity of active ingredient required for effective pest control. Spinosad (Tracer®) was formulated inmaizeflourmatrixgranulesandthreefieldtestscompared10–100ppma.i.granules(equivalentto0.24–2.4ga.i.ha–1)with Tracerasanaqueousspray(200ppma.i.;60ga.i.ha–1),andtherecommendedapplicationratesofBacillusthuringiensis,achemicalandanuntreatedcontrolswereperformed. RESULTS:The100ppmspinosadgranulesresultedinsimilarS.frugiperdamortalitycomparedwiththechemicaltreatmentsin allthreefieldtrials,andresultedinasignificantlyhighermaizegrainyieldcomparedwithunformulatedandcontroltreatments (4141vs.2857and2407kgha–1,respectively)thatwassimilartothechemicaltreatment(3778kgha–1).Bioassaysofgranules stored at room and cold temperatures showed that after 5years, ∼ 70% of the original activity remained (OAR) of spinosad whenformulatedasgranules.Nevertheless,after9years,efficacywasreduced(26.2%and48.5%OAR)atbothroom(25∘C)and refrigeratedtemperatures(4∘C). CONCLUSION:Spinosad,inthegranularphagostimulantformulationsevaluatedinthisstudy,hadadvantagesmeasuredashigh efficacyandlongshelflife