64 research outputs found
Surface expression marker profile in colon cancer cell lines and sphere-derived cells suggests complexity in CD26+ cancer stem cells subsets
Taking advantage of eight established cell lines from colorectal cancer patients at different stages of the disease and the fact that all of them could form spheres, cell surface biomarkers of cancer stem cells and epithelial-mesenchymal transition were tested. The aim was to investigate cancer stem cells and metastatic stem cells in order to provide functional characterization of circulating tumor cells and promote the development of new anti-metastatic therapies. Our model showed an important heterogeneity in EpCAM, CD133, CD44, LGR5, CD26 and E-cadherin expression. We showed the presence of a subset of E-cadherin+ (some cells being E-cadherinhigh) expressing CD26+ (or CD26high) together with the well-known CSC markers LGR5 and EpCAMhigh, sometimes in the absence of CD44 or CD133. The already described CD26+/E-cadherinlow or negative and CD26+/EpCAM−/CD133− subsets were also present. Cell division drastically affected the expression of all markers, in particular E-cadherin, so new-born cells resembled mesenchymal cells in surface staining. CD26 and/or dipeptidyl peptidase 4 inhibitors have already shown anti-metastatic effects in pre-clinical models, and the existence of these CD26+ subsets may help further research against cancer metastasis.This work was done with the Xunta de Galicia grants (supported by the: European
Regional Development Fund (ERDF): Axudas consolidación e estructuración de
unidades de investigación competitiva [GRC2014/019], Galician Network for
Colorectal Cancer Research (REGICC) [R2014/039] and Agrupación estratégica
InBiomed [2012/273]S
Penning-trap eigenfrequency measurements with optical radiofrequency detectors
We use an electric-dipole laser-driven transition to precisely measure the
cyclotron-frequency ratios of the pairs Ca-Ca,
Ca-Ca and Ca-Ca in a 7-tesla
Penning trap. A single laser-cooled (~mK) ion serves, together with
photon-counting and/or photon-imaging units, as a radiofrequency detector
covering a broad-band frequency spectrum, in the present case from kHz to a few
MHz. Such detectors (Ca) allow measuring
extremely small forces, with measured normalized sensitivities down to
yN and yN in the
MHz and kHz regime, respectively. The direct determination of the ions'
amplitudes makes a cyclotron-frequency measurement process more robust against
inhomogeneities of the magnetic field and/or deviations of the electric
quadrupole field due to mechanical imperfections of the trap.Comment: 6 pages, 3 figure
Nucleoside diphosphate kinase A (NM23-H1) as a potential biomarker candidate for colorectal cancer
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Evaluation of pleural effusion sCD26 and DPP-IV as diagnostic biomarkers in lung disease
In this study, we measured ADA and DPP-IV enzymatic activity and sCD26 concentration in 150 pleural
effusion (PE) samples and tested for correlations between these and other cellular and biochemical
measures. We found that DPP-IV in particular might improve the specificity (but not the sensitivity) of the
ADA test for diagnosis of pulmonary tuberculosis, since half of the false ADA positive results in
non-tuberculous PE were also DPP-IV positive. A percentage of patients with malignant PE were sCD26 or
DPP-IV positive; however, some patients with benign PE also tested positive. As a pattern associated with
DPP-IV (but not the CD26 protein) was observed in PE, we searched for a finding that might increase the
value of these biomarkers for diagnosis of malignancy. The observed pattern was related to the presence of
leukocytes, as indicated by correlations with the cell count, and to a band of 180 kDa, detected by
immunoblottingThis research was partially supported by grants PS09-00405 and Research Intensification activity from the Fondo de Investigación Sanitaria (FIS) of the Instituto de Salud Carlos III (Spain) and funding from Xunta de Galicia and FEDER (CN 2011/024). We are grateful to the patients who participated and made the study possibleS
Formation of Two-Ion Crystals by Injection from a Paul-Trap Source into a High-Magnetic-Field Penning Trap
Two-ion crystals constitute a platform for investigations of quantum nature
that can be extended to any ion species or charged particle provided one of the
ions in the crystal can be directly laser-cooled and manipulated with laser
radiation. This paper presents the formation of two-ion crystals for quantum
metrology in a 7-tesla open-ring Penning trap. Ca ions are produced
either internally by photoionization or externally in a (Paul-trap) source,
transported through the strong magnetic field gradient of the superconducting
solenoid, and captured in-flight with a mean kinetic energy of a few
electronvolts with respect to the minimum of the Penning-trap potential well.
Laser cooling of the two-ion crystal in a strong magnetic field towards
reaching the quantum regime is also presented with particular emphasis on the
cooling of the radial modes
In the search of early stages biomarkers for non small cell lung cancer
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