Assessment of dual frequency GNSS observations from a Xiaomi Mi 8 android smartphone and positioning performance analysis

On May 2018 the world’s first dual-frequency Global Navigation Satellite System (GNSS) smartphone produced by Xiaomi equipped with a Broadcom BCM47755 chip was launched. It is able to receive L1/E1/ and L5/E5 signals from GPS, Galileo, Beidou, and GLONASS (GLObal NAvigation Satellite System) satellites. The main aim of this work is to achieve the phone’s position by using multi-constellation, dual frequency pseudorange and carrier phase raw data collected from the smartphone. Furthermore, the availability of dual frequency raw data allows to assess the multipath performance of the device. The smartphone’s performance is compared with that of a geodetic receiver. The experiments were conducted in two different scenarios to test the smartphone under different multipath conditions. Smartphone measurements showed a lower C/N0 and higher multipath compared with those of the geodetic receiver. This produced negative effects on single-point positioning as showed by high root mean square error (RMS). The best positioning accuracy for single point was obtained with the E5 measurements with a DRMS (horizontal root mean square error) of 4.57 m. For E1/L1 frequency, the 2DRMS was 5.36 m. However, the Xiaomi Mi 8, thanks to the absence of the duty cycle, provided carrier phase measurements used for a static single frequency relative positioning with an achieved 2DRMS of 1.02 and 1.95 m in low and high multipath sites, respectively

Precise point positioning with single and dual-frequency multi-GNSS android smartphones

The new generation of Android smartphones is equipped with high performance Global Navigation Satellite System (GNSS) chips capable of tracking dual frequency multi-constellation data. Moreover, starting from version 9 of Android users can disable the duty cycle power saving option thus good quality pseudorange and carrier phase raw data are available thus the application of Precise Point Positioning (PPP) algorithm becomes more and more interesting. The main aim of this work is to assess the PPP performance of the first dual-frequency GNSS smartphone produced by Xiaomi equipped with a Broadcom BCM47755. The advantage of acquire dual frequency data is highlighted by comparing the performance obtained by Xiaomi with that of a single frequency smartphone the Samsung S8. The horizontal and vertical accuracy achieved by Xiaomi are of 0.51 m and 6 m respectively while those achieved by Samsung are 5.64 m for 15 m for horizontal and vertical

Use of the sensors of a latest generation mobile phone for the three-dimensional reconstruction of an archaeological monument: The survey of the Intihuatana stone in Machu Picchu (Peru')

The survey of archaeological monuments presents particular needs and difficulties. Such surveys must in fact be as complete, geometrically correct and accurately geo-referenced as possible. These needs, however, often face problems of difficult accessibility, the need for rapid timing and complex logistical conditions. The latest generation of mobile phones are equipped with ultra-high resolution cameras up to 100 megapixel. Although they do not have the geometric characteristics of professional cameras, they can be used advantageously for the reconstruction of three-dimensional models using Structure from Motion methodologies . At the same time, the latest mobile phones are equipped with GPS/GNSS chips that allow a postprocessing of their positioning allowing to reach decimetric/centimetric accuracies. The use of sensors integrated in a mobile phone greatly simplify the survey both in terms of transportability but also in terms of authorizations by the competent authorities as the equipment is exactly the same that most tourists who visit the sites themselves bring with them. The approach proposed and made possible by these combined features in a latest generation mobile phone have been tested for a rapid survey of the Intihuatana stone in Machu Picchu (Peru), a site with considerable logistical and organizing complexity

Use of the sensors of a latest generation mobile phone for the three-dimensional reconstruction of an archaeological monument: the survey of the Intihuatana stone in Machu Picchu (Peru’)

Abstract The survey of archaeological monuments presents particular needs and difficulties. Such surveys must in fact be as complete, geometrically correct and accurately geo-referenced as possible. These needs, however, often face problems of difficult accessibility, the need for rapid timing and complex logistical conditions. The latest generation of mobile phones are equipped with ultra-high resolution cameras up to 100 megapixel. Although they do not have the geometric characteristics of professional cameras, they can be used advantageously for the reconstruction of three-dimensional models using Structure from Motion methodologies . At the same time, the latest mobile phones are equipped with GPS/GNSS chips that allow a postprocessing of their positioning allowing to reach decimetric/centimetric accuracies. The use of sensors integrated in a mobile phone greatly simplify the survey both in terms of transportability but also in terms of authorizations by the competent authorities as the equipment is exactly the same that most tourists who visit the sites themselves bring with them. The approach proposed and made possible by these combined features in a latest generation mobile phone have been tested for a rapid survey of the Intihuatana stone in Machu Picchu (Peru), a site with considerable logistical and organizing complexity

Solutions and limitations of the geomatic survey of an archaeological site in hard to access areas with a latest generation smartphone: the example of the Intihuatana stone in Machu Picchu (Peru)

Archaeological remains need to be geometrically surveyed and set in absolute reference systems in order to allow a "virtual visit" and to create "digital twins" useful in case of deterioration for proper restoration. Some countries (e.g., Peru) have a vast archaeological heritage whose survey requires optimized procedures that allow high productivity while maintaining high standards of geometric accuracy. A large part of Peru's cultural heritage is located in remote areas, at high altitudes and not easily accessible. For this reason, it is of great interest to study the possible applications of easily transportable instruments. In this study it was verified how the capabilities of the latest smartphones in terms of absolute differential positioning and photogrammetric acquisition can allow the acquisition of a geometrically correct and georeferenced three-dimensional model. The experimentation concerned a new survey of the Intihuatana stones at Machu Picchu and its comparison with a previous survey carried out with a much more complex laser scanning instrumentation. It is important to note that both the photogrammetric survey and the GPS/GNSS survey were carried out with the same smartphone taking full advantage of both features of the same mobile phone. Relative comparison to an existing point cloud provided differences of 2 millimeters in mean with an RMSE of 2 cm. The absolute positioning accuracy compared to a very large-scale cartography appears to be of the order of one metre as was expected mainly due to the high distance of the GPS/GNSS permanent stations

Prexasertib, a Chk1/Chk2 inhibitor, increases the effectiveness of conventional therapy in B-/T- cell progenitor acute lymphoblastic leukemia

During the last few years many Checkpoint kinase 1/2 (Chk1/Chk2) inhibitors have been developed for the treatment of different type of cancers. In this study we evaluated the efficacy of the Chk 1/2 inhibitor prexasertib mesylate monohydrate in B-/T- cell progenitor acute lymphoblastic leukemia (ALL) as single agent and in combination with other drugs. The prexasertib reduced the cell viability in a dose and time dependent manner in all the treated cell lines. The cytotoxic activity was confirmed by the increment of apoptotic cells (Annexin V/Propidium Iodide staining), by the increase of \u3b3H2A.X protein expression and by the activation of different apoptotic markers (Parp-1 and pro-Caspase3 cleavage). Furthermore, the inhibition of Chk1 changed the cell cycle profile. In order to evaluate the chemo-sensitizer activity of the compound, different cell lines were treated for 24 and 48 hours with prexasertib in combination with other drugs (imatinib, dasatinib and clofarabine). The results from cell line models were strengthened in primary leukemic blasts isolated from peripheral blood of adult acute lymphoblastic leukemia patients. In this study we highlighted the mechanism of action and the effectiveness of prexasertib as single agent or in combination with other conventional drugs like imatinib, dasatinib and clofarabine in the treatment of B-/T-ALL

Prevention of incisional hernia post emergency laparotomy: A time to change? A case series.

Introduction: Complicated acute diverticulitis (CAD) is a surgical challenge in which a mini-invasive approach may be offered. Laparoscopic peritoneal lavage (LPL) was introduced as an alternative to sigmoid resection. However, the role of LPL is still under debate. Aim of this study was to evaluate which surgical strategy between LPL and Laparoscopic Sigmoidectomy (LS) could give better outcomes in CAD. Materials and methods: This prospective, observational, multicenter study lasted from 2015 until to 2018. Inclusion criteria: left colonic or sigmoid CAD (modified Hinchey’’s classification: grade II not responder to conservative treatment and grade III). Exclusion criteria: septic shock, immunodepression, previous multiple surgical operations, modified Hinchey’’s grade I and IV,\15 and[85 years. Comparisons were made between LPL and LS groups. Results: 66 patients were enrolled: 28 (42%) had LPL and 38 (58%) LS. Following sigmoidectomy, 24 pts (63%) had a primary anastomosis and 14 pts (37%) an end-colostomy (Hartmann’’s procedure). There were no significant differences regarding age, male gender rate and mean BMI (p = 0.314, p = 0.07, p = 0.129, respectively). ASA score [2 was significantly higher in LPL (p = 0.05). The number of previous episodes of diverticulitis and the mean C-Reactive Protein dosage were similar (p = 0.756 and 0.846). Mannheim Peritonitis Index was significantly higher in LPL (0.004). No differences were found regarding to the distribution of Hinchey’’s grades II and III (p = 0.727). 1 (4%) patient in LPL and 5 pts (13%) in LS needed a conversion to open surgery (p = 0.181). Overall, the morbidity rates were 33% in LPL and 18% in LS (p = 0.169). Organ space infection (30% vs 3%, p = 0.002) and the re-operation rates (18.5% vs 0; p = 0.006) resulted significantly higher in the LPL group. Mortality was nihil. Mean post-op length of stay was 11.4 days in LPL and 8.23 days in LS (p = 0.088). Diverticular recurrence was significantly increased in LPL (p = 0.003). Conclusions: Compared to LS, LPL is associated with increased ongoing sepsis, emergency re-intervention and recurrence of acute diverticulitis. The role of LPL for patients with CAD remains questionabl

Synergism through WEE1 and CHK1 inhibition in acute lymphoblastic leukemia

Introduction: Screening for synthetic lethality markers has demonstrated that the inhibition of the cell cycle checkpoint kinases WEE1 together with CHK1 drastically affects stability of the cell cycle and induces cell death in rapidly proliferating cells. Exploiting this finding for a possible therapeutic approach has showed efficacy in various solid and hematologic tumors, though not specifically tested in acute lymphoblastic leukemia. Methods: The efficacy of the combination between WEE1 and CHK1 inhibitors in B and T cell precursor acute lymphoblastic leukemia (B/T-ALL) was evaluated in vitro and ex vivo studies. The efficacy of the therapeutic strategy was tested in terms of cytotoxicity, induction of apoptosis, and changes in cell cycle profile and protein expression using B/T-ALL cell lines. In addition, the efficacy of the drug combination was studied in primary B-ALL blasts using clonogenic assays. Results: This study reports, for the first time, the efficacy of the concomitant inhibition of CHK1/CHK2 and WEE1 in ALL cell lines and primary leukemic B-ALL cells using two selective inhibitors: PF-0047736 (CHK1/CHK2 inhibitor) and AZD-1775 (WEE1 inhibitor). We showed strong synergism in the reduction of cell viability, proliferation and induction of apoptosis. The efficacy of the combination was related to the induction of early S-phase arrest and to the induction of DNA damage, ultimately triggering cell death. We reported evidence that the efficacy of the combination treatment is independent from the activation of the p53-p21 pathway. Moreover, gene expression analysis on B-ALL primary samples showed that Chek1 and Wee1 are significantly co-expressed in samples at diagnosis (Pearson r = 0.5770, p = 0.0001) and relapse (Pearson r= 0.8919; p = 0.0001). Finally, the efficacy of the combination was confirmed by the reduction in clonogenic survival of primary leukemic B-ALL cells. Conclusion: Our findings suggest that the combination of CHK1 and WEE1 inhibitors may be a promising therapeutic strategy to be tested in clinical trials for adult ALL

Case Report: A Novel Activating FLT3 Mutation in Acute Myeloid Leukemia

FMS-like tyrosine kinase 3 (FLT3) is among the most common driver genes recurrently mutated in acute myeloid leukemia (AML), accounting for approximately 30% of cases. Activating mutations of the FLT3 receptor include internal tandem duplications (ITD) that map to the auto-inhibitory juxtamembrane (JM) domain or point mutations within the tyrosine kinase domain (TKD). Several FLT3 tyrosine kinase inhibitors have been developed in the last few years, but midostaurin is currently the only one approved for the treatment of newly diagnosed patients harboring FLT3 mutations. Here we describe for the first time a novel in-frame deletion in exon 14 (JM domain) of the FLT3 gene, that we identified in a young woman with CBFb-MYH11-positive AML. We demonstrated that this novel FLT3 variant is pathogenic, since it is responsible for constitutive activation of FLT3 receptor. Finally, ex-vivo studies demonstrated that this novel mutation is sensitive to midostaurin

Identification of Two DNMT3A Mutations Compromising Protein Stability and Methylation Capacity in Acute Myeloid Leukemia

Somatic mutations of DNMT3A occur in about 20% of acute myeloid leukemia (AML) patients. They mostly consist in heterozygous missense mutations targeting a hotspot site at R882 codon, which exhibit a dominant negative effect and are associated with high myeloblast count, advanced age, and poor prognosis. Other types of mutations such as truncations, insertions, or single-nucleotide deletion also affect the DNMT3A gene, though with lower frequency. The present study aimed to characterize two DNMT3A gene mutations identified by next-generation sequencing (NGS), through analysis of protein stability and DNA methylation status at CpG islands. The first mutation was a single-nucleotide variant of DNMT3A at exon 20 causing a premature STOP codon (c.2385G > A; p.Trp795 17; NM-022552.4). The DNMT3A mutation load increased from 4.5% to 38.2% during guadecitabine treatment, with a dominant negative effect on CpG methylation and on protein expression. The second mutation was a novel insertion of 35 nucleotides in exon 22 of DNMT3A (NM-022552.4) that introduced a STOP codon too, after the amino acid Glu863 caused by a frameshift insertion (c.2586-2587insTCATGAATGAGAAAGAGGACATCTTATGGTGCACT; p. Thr862-Glu863fsins). The mutation, which was associated with reduced DNMT3A expression and CpG methylation, persisted at relapse with minor changes in the methylation profile and at protein level. Our data highlight the need to better understand the consequences of DNMT3A mutations other than R882 substitutions in the leukemogenic process in order to tailor patient treatments, thus avoiding therapeutic resistance and disease relapse