Assessing the potential for egg chemoattractants to mediate sexual selection in a broadcast spawning marine invertebrate

In numerous species, egg chemoattractants play a critical role in guiding sperm towards unfertilized eggs (sperm chemotaxis). Until now, the known functions of sperm chemotaxis include increasing the effective target size of eggs, thereby promoting sperm–egg encounters, and facilitating species recognition. Here, we report that in the broadcast spawning mussel, Mytilus galloprovincialis, egg chemoattractants may play an unforeseen role in sexual selection by enabling sperm to effectively ‘choose’ between the eggs of different conspecific females. In an initial experiment, we confirmed that sperm chemotaxis occurs in M. galloprovincialis by showing that sperm are attracted towards unfertilized eggs when given the choice of eggs or no eggs in a dichotomous chamber. We then conducted two cross-classified mating experiments, each comprising the same individual males and females crossed in identical male × female combinations, but under experimental conditions that offered sperm ‘no-choice’ (each fertilization trial took place in a Petri dish and involved a single male and female) or a ‘choice’ of a female's eggs (sperm were placed in the centre of a dichotomous choice chamber and allowed to choose eggs from different females). We show that male-by-female interactions characterized fertilization rates in both experiments, and that there was remarkable consistency between patterns of sperm migration in the egg-choice experiment and fertilization rates in the no-choice experiment. Thus, sperm appear to exploit chemical cues to preferentially swim towards eggs with which they are most compatible during direct sperm-to-egg encounters. These results reveal that sperm differentially select eggs on the basis of chemical cues, thus exposing the potential for egg chemoattractants to mediate mate choice for genetically compatible partners. Given the prevalence of sperm chemotaxis across diverse taxa, our findings may have broad implications for sexual selection in other mating systems

A Systems Approach to Developing an Affordable Space Ground Transportation Architecture using a Commonality Approach

This paper presents a structured approach for achieving a compatible Ground System (GS) and Flight System (FS) architecture that is affordable, productive and sustainable. This paper is an extension of the paper titled "Approach to an Affordable and Productive Space Transportation System" by McCleskey et al. This paper integrates systems engineering concepts and operationally efficient propulsion system concepts into a structured framework for achieving GS and FS compatibility in the mid-term and long-term time frames. It also presents a functional and quantitative relationship for assessing system compatibility called the Architecture Complexity Index (ACI). This paper: (1) focuses on systems engineering fundamentals as it applies to improving GS and FS compatibility; (2) establishes mid-term and long-term spaceport goals; (3) presents an overview of transitioning a spaceport to an airport model; (4) establishes a framework for defining a ground system architecture; (5) presents the ACI concept; (6) demonstrates the approach by presenting a comparison of different GS architectures; and (7) presents a discussion on the benefits of using this approach with a focus on commonality

SplicerAV: a tool for mining microarray expression data for changes in RNA processing

<p>Abstract</p> <p>Background</p> <p>Over the past two decades more than fifty thousand unique clinical and biological samples have been assayed using the Affymetrix HG-U133 and HG-U95 GeneChip microarray platforms. This substantial repository has been used extensively to characterize changes in gene expression between biological samples, but has not been previously mined <it>en masse </it>for changes in mRNA processing. We explored the possibility of using HG-U133 microarray data to identify changes in alternative mRNA processing in several available archival datasets.</p> <p>Results</p> <p>Data from these and other gene expression microarrays can now be mined for changes in transcript isoform abundance using a program described here, SplicerAV. Using <it>in vivo </it>and <it>in vitro </it>breast cancer microarray datasets, SplicerAV was able to perform both gene and isoform specific expression profiling within the same microarray dataset. Our reanalysis of Affymetrix U133 plus 2.0 data generated by <it>in vitro </it>over-expression of HRAS, E2F3, beta-catenin (CTNNB1), SRC, and MYC identified several hundred oncogene-induced mRNA isoform changes, one of which recognized a previously unknown mechanism of <it>EGFR </it>family activation. Using clinical data, SplicerAV predicted 241 isoform changes between low and high grade breast tumors; with changes enriched among genes coding for guanyl-nucleotide exchange factors, metalloprotease inhibitors, and mRNA processing factors. Isoform changes in 15 genes were associated with aggressive cancer across the three breast cancer datasets.</p> <p>Conclusions</p> <p>Using SplicerAV, we identified several hundred previously uncharacterized isoform changes induced by <it>in vitro </it>oncogene over-expression and revealed a previously unknown mechanism of EGFR activation in human mammary epithelial cells. We analyzed Affymetrix GeneChip data from over 400 human breast tumors in three independent studies, making this the largest clinical dataset analyzed for <it>en masse </it>changes in alternative mRNA processing. The capacity to detect RNA isoform changes in archival microarray data using SplicerAV allowed us to carry out the first analysis of isoform specific mRNA changes directly associated with cancer survival.</p

Galaxy Distances in the Nearby Universe: Corrections For Peculiar Motions

By correcting the redshift--dependent distances for peculiar motions through a number of peculiar velocity field models, we recover the true distances of a wide, all-sky sample of nearby galaxies (~ 6400 galaxies with velocities cz<5500 km/s), which is complete up to the blue magnitude B=14 mag. Relying on catalogs of galaxy groups, we treat ~2700 objects as members of galaxy groups and the remaining objects as field galaxies. We model the peculiar velocity field using: i) a cluster dipole reconstruction scheme; ii) a multi--attractor model fitted to the Mark II and Mark III catalogs of galaxy peculiar velocities. According to Mark III data the Great Attractor has a smaller influence on local dynamics than previously believed, whereas the Perseus-Pisces and Shapley superclusters acquire a specific dynamical role. Remarkably, the Shapley structure, which is found to account for nearly half the peculiar motion of the Local Group, is placed by Mark III data closer to the zone of avoidance with respect to its optical position. Our multi--attractor model based on Mark III data favors a cosmological density parameter Omega ~ 0.5 (irrespective of a biasing factor of order unity). Differences among distance estimates are less pronounced in the ~ 2000 - 4000 km/s distance range than at larger or smaller distances. In the last regions these differences have a serious impact on the 3D maps of the galaxy distribution and on the local galaxy density --- on small scales.Comment: 24 pages including (9 eps figures and 7 tables). Figures 1,2,3,4 are available only upon request. Accepted by Ap

The function of FGF signaling in the lens placode

AbstractPrevious studies suggested that FGF signaling is important for lens formation. However, the times at which FGFs act to promote lens formation, the FGFs that are involved, the cells that secrete them and the mechanisms by which FGF signaling may promote lens formation are not known. We found that transcripts encoding several FGF ligands and the four classical FGF receptors are detectable in the lens-forming ectoderm at the time of lens induction. Conditional deletion of Fgfr1 and Fgfr2 from this tissue resulted in the formation of small lens rudiments that soon degenerated. Lens placodes lacking Fgfr1 and 2 were thinner than in wild-type embryos. Deletion of Fgfr2 increased cell death from the initiation of placode formation and concurrent deletion of Fgfr1 enhanced this phenotype. Fgfr1/2 conditional knockout placode cells expressed lower levels of proteins known to be regulated by FGF receptor signaling, but proteins known to be important for lens formation were present at normal levels in the remaining placode cells, including the transcription factors Pax6, Sox2 and FoxE3 and the lens-preferred protein αA-crystallin. Previous studies identified a genetic interaction between BMP and FGF signaling in lens formation and conditional deletion of Bmpr1a caused increased cell death in the lens placode, resulting in the formation of smaller lenses. In the present study, conditional deletion of both Bmpr1a and Fgfr2 increased cell death beyond that seen in Fgfr2CKO placodes and prevented lens formation. These results suggest that the primary role of autocrine or paracrine FGF signaling is to provide essential survival signals to lens placode cells. Because apoptosis was already increased at the onset of placode formation in Fgfr1/2 conditional knockout placode cells, FGF signaling was functionally absent during the period of lens induction by the optic vesicle. Since the expression of proteins required for lens formation was not altered in the knockout placode cells, we can conclude that FGF signaling from the optic vesicle is not required for lens induction

Effects of alternative bedding substrates on lamb welfare, productive performance, and meat quality during the finishing phase of fattening

The study analyzed the effect of using sawdust, cellulose, straw, and rice husk bedding on the welfare, performance and meat quality of lambs during the finishing phase of fattening. Ninety-six lambs (fed ad libitum with concentrate) were assigned to pens containing 1 of 4 bedding materials in groups of 12 for 14 days. Live weight and feed consumption were recorded to estimate daily gain and conversion index, and carcass and meat quality variables were measured. Stereotypies, exploratory interactions with bedding material, and agonistic and affiliative interactions between animals were recorded by continuous sampling techniques using video recording. The substrates affected behavior and stress response variables. There were more stereotypies and agonistic interactions at the beginning of the experiment in all treatments (P ≤ 0.05). The lambs fattened on cellulose bedding interacted more with the substrate throughout the finishing stage (P ≤ 0.05), where there were also more affiliative interactions (P ≤ 0.05). Stress levels were lower in lambs on straw, followed by cellulose. No significant differences in production or carcass and meat quality traits were observed between treatments. The results suggest that all substrates evaluated could be efficiently incorporated into the system as bedding material without affecting productivity or product quality

An Experimental Platform for Pulsed-Power Driven Magnetic Reconnection

We describe a versatile pulsed-power driven platform for magnetic reconnection experiments, based on exploding wire arrays driven in parallel [Suttle, L. G. et al. PRL, 116, 225001]. This platform produces inherently magnetised plasma flows for the duration of the generator current pulse (250 ns), resulting in a long-lasting reconnection layer. The layer exists for long enough to allow evolution of complex processes such as plasmoid formation and movement to be diagnosed by a suite of high spatial and temporal resolution laser-based diagnostics. We can access a wide range of magnetic reconnection regimes by changing the wire material or moving the electrodes inside the wire arrays. We present results with aluminium and carbon wires, in which the parameters of the inflows and the layer which forms are significantly different. By moving the electrodes inside the wire arrays, we change how strongly the inflows are driven. This enables us to study both symmetric reconnection in a range of different regimes, and asymmetric reconnection.Comment: 14 pages, 9 figures. Version revised to include referee's comments. Submitted to Physics of Plasma

Correlated X-ray and Optical Variability in V404 Cyg in Quiescence

We report simultaneous X-ray and optical observations of V404 Cyg in quiescence. The X-ray flux varied dramatically by a factor of >20 during a 60ks observation. X-ray variations were well correlated with those in Halpha, although the latter include an approximately constant component as well. Correlations can also be seen with the optical continuum, although these are less clear. We see no large lag between X-ray and optical line variations; this implies they are causally connected on short timescales. As in previous observations, Halpha flares exhibit a double-peaked profile suggesting emission distributed across the accretion disk. The peak separation is consistent with material extending outwards to at least the circularization radius. The prompt response in the entire Halpha line confirms that the variability is powered by X-ray (and/or EUV) irradiation.Comment: 5 pages; Accepted for publication in the Astrophysical Journal Letter