Ultrastructure of Coronary Arterial Endothelium in Atherosclerotic Swine Suggests Lipid Retro-Endocytosis

During experimental atherosclerosis, arterial endothelial cells show characteristic ultrastructural changes including the appearance of increased numbers of plasmalemmal and cytoplasmic vesicles. These structures have been shown by tracer studies to participate in the transcellular transport of low-density lipoprotein cholesterol and β-very-low-density lipoprotein cholesterol from the arterial lumen into the abluminal extracellular matrix. Although this probably represents the major lipid transport pathway, other forms of transport may exist. We document the presence of apparent lipid structures averaging approximately 300 nm in diameter at or near the luminal surface of coronary arterial endothelium of atherosclerotic miniature swine. The structures exhibited a particulate nature with subunits of a heterogenous size distribution. The appearance of the endothelial plasmalemma adjacent and subjacent to these structures suggests exocytosis. We hypothesize that this previously unreported morphology may represent an in vivo structural correlate for the lipoprotein retro-endocytosis pathway which has been recently identified using biochemical methods in smooth muscle cells and fibroblasts in vitro

Whole-genome sequence analysis shows that two endemic species of North American wolf are admixtures of the coyote and gray wolf.

Protection of populations comprising admixed genomes is a challenge under the Endangered Species Act (ESA), which is regarded as the most powerful species protection legislation ever passed in the United States but lacks specific provisions for hybrids. The eastern wolf is a newly recognized wolf-like species that is highly admixed and inhabits the Great Lakes and eastern United States, a region previously thought to be included in the geographic range of only the gray wolf. The U.S. Fish and Wildlife Service has argued that the presence of the eastern wolf, rather than the gray wolf, in this area is grounds for removing ESA protection (delisting) from the gray wolf across its geographic range. In contrast, the red wolf from the southeastern United States was one of the first species protected under the ESA and was protected despite admixture with coyotes. We use whole-genome sequence data to demonstrate a lack of unique ancestry in eastern and red wolves that would not be expected if they represented long divergent North American lineages. These results suggest that arguments for delisting the gray wolf are not valid. Our findings demonstrate how a strict designation of a species under the ESA that does not consider admixture can threaten the protection of endangered entities. We argue for a more balanced approach that focuses on the ecological context of admixture and allows for evolutionary processes to potentially restore historical patterns of genetic variation

Viruses and Drug Resistant Bacteria in Groundwater of Southeastern Minnesota

ABSTRACT-Karst topography of soils in southeastern Minnesota is responsible for the formation of sinkholes, subsurface cracks, and underground rivers which may enhance the transportation of surface contaminants into groundwater. The present study was conducted to determine the presence of human pathogenic viruses, coliforms, fecal coliforms and coliphages in private rural wells of this area. The occurrence of drug resistance in bacteria isolated from groundwater also was studied. Coliform bacteria were detected at least once from 22 of the 26 sites sampled over 34 months. Water from 10 sites yielded drug-resistant indicator bacteria; 25 of 38 (65.8 percent) total coliforms and 9 of27 (33.3 percent) fecal coliforms tested were found to carry drug resistance. Human enteric viruses were detected by DNA hybridization and/or virus isolation techniques in nine samples from seven different sites, some in the absence of fecal coliforms. Of the 161 samples tested for coliphages, 13 samples from seven sites were found positive. On two occasions, coliphages were isolated from samples in which coliforms were absent. These findings indicate that potential public health problems exist in this region

Cornering characteristics of the main-gear tire of the space shuttle orbiter

An experimental investigation was conducted at the NASA Langley Research Center to study the effects of various vertical load and yaw angle conditions on the cornering behavior of the Space Shuttle Orbiter main gear tire. Measured parameters included side and drag force, side and drag force coefficients, aligning torque, and overturning torque. Side force coefficient was found to increase as yaw angle was increased, but decreased as the vertical load was increased. Drag force was found to increase as vertical load was increased at constant yaw angles. Aligning torque measurements indicated that the tire is stable in yaw

Euler-Bessel and Euler-Fourier Transforms

We consider a topological integral transform of Bessel (concentric isospectral sets) type and Fourier (hyperplane isospectral sets) type, using the Euler characteristic as a measure. These transforms convert constructible \zed-valued functions to continuous $\real$-valued functions over a vector space. Core contributions include: the definition of the topological Bessel transform; a relationship in terms of the logarithmic blowup of the topological Fourier transform; and a novel Morse index formula for the transforms. We then apply the theory to problems of target reconstruction from enumerative sensor data, including localization and shape discrimination. This last application utilizes an extension of spatially variant apodization (SVA) to mitigate sidelobe phenomena

Flight Studies of Problems Pertinent to Low-Speed Operation of Jet Transports

Flight studies have been made of the low-speed operational regime of jet transports in order to assess potential operating problems. The study was performed utilizing a large multiengine jet airplane having geometric characteristics fairly representative of the jet transports; however, to insure general applicability of the results, the aerodynamic characteristics of the test airplane were varied to simulate a variety of jet-transport airplanes. The specific areas investigated include those of the take-off and landing, and the relation of these maneuvers to the 1 g stall speed and stalling characteristics. The take-off studies included evaluation of the factors affecting the take-off speed and attitude, including the effects of premature rotation and of over-rotation on ground run required. The approach and landing studies pertained to such factors as: desirable lateral-directional damping characteristics; lateral-control requirements; space-positioning limitations during approach under VFR or IFR conditions and requirements for glide-path controls; and evaluation of factors affecting the pilot's choice of landing speeds. Specific recommendations and some indication of desirable characteristics for the jet transports are advanced to alleviate possible operational difficulties or to improve operational performance in the low-speed range

HST Imaging Polarimetry of the Gravitational Lens FSC10214+4724

We present imaging polarimetry of the extremely luminous, redshift 2.3 IRAS source FSC10214+4724. The observations were obtained with HST's Faint Object Camera in the F437M filter, which is free of strong emission lines. The 0.7 arcsec long arc is unresolved to 0.04 arcsec FWHM in the transverse direction, and has an integrated polarization of 28 +/- 3 percent, in good agreement with ground-based observations. The polarization position angle varies along the arc by up to 35 deg. The overall position angle is 62 +/- 3 deg east of north. No counterimage is detected to B = 27.5 mag ($3\sigma$), giving an observed arc to counterimage flux ratio greater than 250, considerably greater than the flux ratio of 100 measured previously in the I-band. This implies that the configuration of the object in the source plane at the B-band is different from that at I-band, and/or that the lensing galaxy is dusty.Comment: 17 pages, 3 figures. Accepted for publication in Astronomical Journal, February 199

Enhanced 'In-situ' catalysis via microwave selective heating: catalytic chain transfer polymerisation

An extremely facile, single stage, ‘in-situ’, Catalytic Chain Transfer Polymerisation (CCTP) process has been identified, where the optimal polymerisation process was shown to depend upon a combination of catalyst characteristics (i.e. solubility, sensitivity, activity) and the method of heating applied. In comparison to the current benchmark catalyst, the preparation of which is only about 40 % efficient, this represents a significant increase in waste prevention/atom efficiency and removes the need for organic solvent. It was also shown possible to significantly reduce the overall ‘in-situ’ reaction cycle time by adopting different processing strategies in order to minimise energy use. The application of microwave heating was demonstrated to overcome system diffusion/dilution issues and result in rapid, ‘in-situ’ catalyst formation. This allowed processing times to be minimised by enabling a critical concentration of the species susceptible to microwave selective heating to dominate the heat and mass transfer involved

Civil conflict, federalism and strategic delegation of leadership

This article analyzes negative externalities that policymakers in one region or group may impose upon the citizens of neighboring regions or groups. These externalities may be material, but they may also be psychological (in the form of envy). The latter form of externality may arise from the production of 'conspicuous' public goods. As a result, decentralized provision of conspicuous public goods may be too high. Potentially, a centralized legislature may internalize negative externalities. However, in a model with strategic delegation, we argue that the median voter in each jurisdiction may anticipate a reduction in local public goods supply and delegate to a policymaker who cares more for public goods than she does herself. This last effect mitigates the expected benefits of policy centralization. The authors' theory is then applied to the setting of civil conflict, where they discuss electoral outcomes in Northern Ireland and Yugoslavia before and after significant institutional changes that affected the degree of centralization. These case studies provide support for the authors' theoretical predictions

Processing and Transmission of Information

Contains research objectives and summary of research on three research projects and reports on two research projects.National Aeronautics and Space Administration (Grant NGL 22-009-013)National Science Foundation (Grant GK-41464)National Science Foundation (Grant GK-41098)Joint Services Electronics Program (Contract DAAB07-74-C-0630)National Science Foundation (Grant GK-37582