970 research outputs found
Non-alcoholic fatty liver disease: relationship with cardiovascular risk markers and clinical endpoints
Non-alcoholic fatty liver disease (NAFLD) is a common diagnosis and is increasing in prevalence worldwide. NAFLD is usually asymptomatic at presentation; progression of the
disease is unpredictable, leading to the development of a variety of techniques for screening, diagnosis and risk stratification. Clinical methods in current use include serum biomarker panels, hepatic ultrasound, magnetic resonance imaging, and liver biopsy.
NAFLD is strongly associated with the metabolic syndrome, and the most common cause of death for people with the condition is cardiovascular disease. Whether NAFLD is an independent cardiovascular risk factor needs exploration. NAFLD has been associated with surrogate markers of cardiovascular disease such as carotid intima-media thickness, the presence
of carotid plaque, brachial artery vasodilatory responsiveness and CT coronary artery
calcification score.
There is no effective medical treatment for NAFLD and evidence is lacking regarding the efficacy of interventions in mitigating cardiovascular risk. Health care professionals managing patients with NAFLD should tackle the issue with early identification of risk factors and aggressive modification. Current management strategies therefore comprise lifestyle change,with close attention to known cardiovascular risk factors
Liver fat in adults with GH deficiency: comparison to matched controls and the effect of GH replacement
CONTEXT:
Existing data regarding the association between growth hormone deficiency (GHD) and liver fat content are conflicting.
OBJECTIVE:
We aimed i) to assess intrahepatocellular lipid (IHCL) content in hypopituitary adults with GHD compared to matched controls and ii) to evaluate the effect of growth hormone (GH) replacement on IHCL content.
DESIGN:
Cross-sectional comparison and controlled intervention study.
PATIENTS, PARTICIPANTS:
Cross-sectional comparison: 22 hypopituitary adults with GHD and 44 healthy controls matched for age, BMI, gender and ethnicity. Intervention study: 9 GHD patients starting GH replacement (GH Rx group), 9 GHD patients not starting replacement therapy (non-GH Rx group).
INTERVENTION:
Intervention study:GH replacement for 6 months in the GH Rx group, dosage was titrated to achieve normal IGF-1 levels.
MAIN OUTCOME MEASURES:
IHCL content determined by proton magnetic resonance spectroscopy (1 H MRS).
RESULTS:
Cross-sectional comparison: There was no difference in IHCL content between GHD patients and healthy controls (1.89% (0.30, 4.03) vs. 1.14% (0.22, 2.32); p=0.2), the prevalence of patients with hepatic steatosis (IHCL of ≥ 5.56%) was similar in the two groups (22.7% vs. 15.9%; chi square probability = 0.4). Intervention study: The change in IHCL content over 6 months did not differ between the GH Rx group and the non-GH Rx group (-0.63 ± 4.53% vs. +0.11 ± 1.46%; p=0.6).
CONCLUSIONS:
In our study liver fat content and the prevalence of hepatic steatosis did not differ between hypopituitary adults with GHD and matched controls. In GHD patients GH replacement had no effect on liver fat content
Infection with the hepatitis C virus causes viral genotype-specific differences in cholesterol metabolism and hepatic steatosis
Lipids play essential roles in the hepatitis C virus (HCV) life cycle and patients with chronic HCV infection display disordered lipid metabolism which resolves following successful anti-viral therapy. It has been proposed that HCV genotype 3 (HCV-G3) infection is an independent risk factor for hepatocellular carcinoma and evidence suggests lipogenic proteins are involved in hepatocarcinogenesis. We aimed to characterise variation in host lipid metabolism between participants chronically infected with HCV genotype 1 (HCV-G1) and HCV-G3 to identify likely genotype-specific differences in lipid metabolism. We combined several lipidomic approaches: analysis was performed between participants infected with HCV-G1 and HCV-G3, both in the fasting and non-fasting states, and after sustained virological response (SVR) to treatment. Sera were obtained from 112 fasting patients (25% with cirrhosis). Serum lipids were measured using standard enzymatic methods. Lathosterol and desmosterol were measured by gas-chromatography mass spectrometry (MS). For further metabolic insight on lipid metabolism, ultra-performance liquid chromatography MS was performed on all samples. A subgroup of 13 participants had whole body fat distribution determined using in vivo magnetic resonance imaging and spectroscopy. A second cohort of (non-fasting) sera were obtained from HCV Research UK for comparative analyses: 150 treatment naïve patients and 100 non-viraemic patients post-SVR. HCV-G3 patients had significantly decreased serum apoB, non-HDL cholesterol concentrations, and more hepatic steatosis than those with HCV-G1. HCV-G3 patients also had significantly decreased serum levels of lathosterol, without significant reductions in desmosterol. Lipidomic analysis showed lipid species associated with reverse cholesterol transport pathway in HCV-G3. We demonstrated that compared to HCV-G1, HCV-G3 infection is characterised by low LDL cholesterol levels, with preferential suppression of cholesterol synthesis via lathosterol, associated with increasing hepatic steatosis. The genotype-specific lipid disturbances may shed light on genotypic variations in liver disease progression and promotion of hepatocellular cancer in HCV-G3
Infection with the hepatitis C virus causes viral genotype-specific differences in cholesterol metabolism and hepatic steatosis
Background: Lipids play essential roles in the hepatitis C virus (HCV) life cycle and patients with chronic HCV infection display disordered lipid metabolism which resolves following successful anti-viral therapy. It has been proposed that HCV genotype 3 (HCV-G3) infection is an independent risk factor for hepatocellular carcinoma and evidence suggests lipogenic proteins are involved in hepatocarcinogenesis. Aims: We aimed to characterise variation in host lipid metabolism between participants chronically infected with HCV genotype 1 (HCV-G1) and HCV-G3 to identify likely genotype-specific differences in lipid metabolism. Methods: We combined several lipidomic approaches: analysis was performed between participants infected with HCV-G1 and HCV-G3, both in the fasting and non-fasting states, and after sustained virological response (SVR) to treatment. Sera were obtained from 112 fasting patients (25% with cirrhosis). Serum lipids were measured using standard enzymatic methods. Lathosterol and desmosterol were measured by gas-chromatography mass spectrometry (MS). For further metabolic insight on lipid metabolism, ultra-performance liquid chromatography MS was performed on all samples. A subgroup of 13 participants had whole body fat distribution determined using in vivo magnetic resonance imaging and spectroscopy. A second cohort of (non-fasting) sera were obtained from HCV Research UK for comparative analyses: 150 treatment naïve patients and 100 non-viraemic patients post-SVR. Results: HCV-G3 patients had significantly decreased serum apoB, non-HDL cholesterol concentrations, and more hepatic steatosis than those with HCV-G1. HCV-G3 patients also had significantly decreased serum levels of lathosterol, without significant reductions in desmosterol. Lipidomic analysis showed lipid species associated with reverse cholesterol transport pathway in HCV-G3. Conclusions: We demonstrated that compared to HCV-G1, HCV-G3 infection is characterised by low LDL cholesterol levels, with preferential suppression of cholesterol synthesis via lathosterol, associated with increasing hepatic steatosis. The genotype-specific lipid disturbances may shed light on genotypic variations in liver disease progression and promotion of hepatocellular cancer in HCV-G3
Magnetic trapping of metastable atomic strontium
We report the magnetic trapping of metastable atomic strontium. Atoms
are cooled in a magneto-optical trap (MOT) operating on the dipole allowed
transition at 461 nm. Decay via
continuously loads a magnetic trap formed by the quadrupole magnetic field of
the MOT. Over atoms at a density of cm and
temperature of 1 mK are trapped. The atom temperature is significantly lower
than what would be expected from the kinetic and potential energy of atoms as
they are transferred from the MOT. This suggests that thermalization and
evaporative cooling are occurring in the magnetic trap.Comment: This paper has been accepted by PR
The Phospho-Dependent Dynamin-Syndapin Interaction Triggers Activity-Dependent Bulk Endocytosis of Synaptic Vesicles
Synaptic vesicles (SVs) are retrieved by more than one mode in central nerve terminals. During mild stimulation, the dominant SV retrieval pathway is classical clathrin-mediated endocytosis (CME). During elevated neuronal activity, activity-dependent bulk endocytosis (ADBE) predominates, which requires activation of the calcium-dependent protein phosphatase calcineurin. We now report that calcineurin dephosphorylates dynamin I in nerve terminals only above the same activity threshold that triggers ADBE. ADBE was arrested when the two major phospho-sites on dynamin I were perturbed, suggesting that dynamin I dephosphorylation is a key step in its activation. Dynamin I dephosphorylation stimulates a specific dynamin I-syndapin I interaction. Inhibition of this interaction by competitive peptides or by site-directed mutagenesis exclusively inhibited ADBE but did not affect CME. The results reveal that the phospho-dependent dynamin-syndapin interaction recruits ADBE to massively increase SV endocytosis under conditions of elevated neuronal activity
The Void Abundance with Non-Gaussian Primordial Perturbations
We use a Press-Schechter-like calculation to study how the abundance of voids
changes in models with non-Gaussian initial conditions. While a positive
skewness increases the cluster abundance, a negative skewness does the same for
the void abundance. We determine the dependence of the void abundance on the
non-Gaussianity parameter fnl for the local-model bispectrum-which approximates
the bispectrum in some multi-field inflation models-and for the equilateral
bispectrum, which approximates the bispectrum in e.g. string-inspired DBI
models of inflation. We show that the void abundance in large-scale-structure
surveys currently being considered should probe values as small as fnl < 10 and
fnl^eq < 30, over distance scales ~10 Mpc.Comment: Submitted to JCA
On the accretion disc properties in eclipsing dwarf nova EM Cyg
In this paper we analyzed the behavior of the unusual dwarf nova EM Cyg using
the data obtained in April-October, 2007 in Vyhorlat observatory (Slovak
Republic) and in September, 2006 in Crimean Astrophysical Observatory
(Ukraine). During our observations EM Cyg has shown outbursts in every 15-40
days. Because on the light curves of EM Cyg the partial eclipse of an accretion
disc is observed we applied the eclipse mapping technique to reconstruct the
temperature distribution in eclipsed parts of the disc. Calculations of the
accretion rate in the system were made for the quiescent and the outburst
states of activity for different distances.Comment: 6 pages, 3 figures, accepted in Astrophysics and Space Scienc
Optical and X-ray correlations during the 2015 outburst of the black hole V404 Cyg
We present a serendipitous multiwavelength campaign of optical photometry simultaneous with Integral X-ray monitoring of the 2015 outburst of the black hole V404 Cyg. Large-amplitude optical variability is generally correlated with X-rays, with lags of order a minute or less compatible with binary light travel time-scales or jet ejections. Rapid optical flaring on time-scales of seconds or less is incompatible with binary light-travel time-scales and has instead been associated with synchrotron emission from a jet. Both this rapid jet response and the lagged and smeared one can be present simultaneously. The optical brightness is not uniquely determined by the X-ray brightness, but the X-ray/optical relationship is bounded by a lower envelope such that at any given optical brightness there is a maximum X-ray brightness seen. This lower envelope traces out a Fopt∝F0.54X relation that can be approximately extrapolated back to quiescence. Rapid optical variability is only seen near this envelope, and these periods correspond to the hardest hard X-ray colours. This correlation between hard X-ray colour and optical variability (and anticorrelation with optical brightness) is a novel finding of this campaign, and apparently a facet of the outburst behaviour in V404 Cyg. It is likely that these correlations are driven by changes in the central accretion rate and geometry
A Year in the Life of the EU-CardioRNA COST Action: CA17129 Catalysing Transcriptomics Research in Cardiovascular Disease
The EU-CardioRNA Cooperation in Science and Technology (COST) Action is a European-wide consortium established in 2018 with 31 European country members and four associate member countries to build bridges between translational researchers from academia and industry who conduct research on non-coding RNAs, cardiovascular diseases and similar research areas. EU-CardioRNA comprises four core working groups (WG1-4). In the first year since its launch, EU-CardioRNA met biannually to exchange and discuss recent findings in related fields of scientific research, with scientific sessions broadly divided up according to WG. These meetings are also an opportunity to establish interdisciplinary discussion groups, brainstorm ideas and make plans to apply for joint research grants and conduct other scientific activities, including knowledge transfer. Following its launch in Brussels in 2018, three WG meetings have taken place. The first of these in Lisbon, Portugal, the second in Istanbul, Turkey, and the most recent in Maastricht, The Netherlands. Each meeting includes a scientific session from each WG. This meeting report briefly describes the highlights and key take-home messages from each WG session in this first successful year of the EU-CardioRNA COST Action. © 2020 by the authors
- …