301 research outputs found
Impact of Oxidation State on Reactivity and Selectivity Differences between Nickel(III) and Nickel(IV) Alkyl Complexes
Described is a systematic comparison of factors impacting the relative rates and selectivities of C(sp3)âC and C(sp3)âO bondâforming reactions at highâvalent Ni as a function of oxidation state. Two Ni complexes are compared: a cationic octahedral NiIV complex ligated by tris(pyrazolyl)borate and a cationic octahedral NiIII complex ligated by tris(pyrazolyl)methane. Key features of reactivity/selectivity are revealed: 1)â
C(sp3)âC(sp2) bondâforming reductive elimination occurs from both centers, but the NiIII complex reacts up to 300âfold faster than the NiIV, depending on the reaction conditions. The relative reactivity is proposed to derive from ligand dissociation kinetics, which vary as a function of oxidation state and the presence/absence of visible light. 2)â
Upon the addition of acetate (AcOâ), the NiIV complex exclusively undergoes C(sp3)âOAc bond formation, while the NiIII analogue forms the C(sp3)âC(sp2) coupled product selectively. This difference is rationalized based on the electrophilicity of the respective MâC(sp3) bonds, and thus their relative reactivity towards outerâsphere SN2âtype bondâforming reactions.The high point: This report describes a systematic comparison of factors impacting the relative rates and selectivities of C(sp3)âC and C(sp3)âO bondâforming reactions at highâvalent Ni centers as a function of oxidation state (NiIII versus NiIV). Two Ni complexes are compared: a cationic octahedral NiIV complex ligated by tris(pyrazolyl)borate and a cationic octahedral NiIII complex ligated by tris(pyrazolyl)methane.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150547/1/anie201903638.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150547/2/anie201903638-sup-0001-misc_information.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150547/3/anie201903638_am.pd
Multiâscale effects of land cover, weather, and fire on Columbian sharpâtailed grouse
Columbian sharpâtailed grouse (Tympanuchus phasianellus columbianus) are endemic to grassland and shrubâsteppe ecosystems of western North America, yet their distribution has contracted to \u3c10% of their historical range. Primary threats to Columbian sharpâtailed grouse include loss of native habitat and conversion to agriculture, reductions in habitat once provided by the Conservation Reserve Program (CRP), wildfire, and drought conditions, yet populationâlevel consequences of these threats and their spatioâtemporal scales of effect are poorly understood. We evaluated multiâscale effects of land cover, weather, and fire histories on patterns of abundance and productivity for Columbian sharpâtailed grouse populations during 1995â2020 in Idaho, USA, using mixedeffects generalized regression and remotely sensed data. We demonstrated negative effects of fire, tree encroachment, and bare ground, positive effects of spring and summer precipitation and cover of shrubs and perennial forbs and grasses, and positive effects of CRP on grouse abundance that changed in magnitude with cover of perennials and shrubs near leks (i.e., strongest effects when average cover of shrubs and perennial forbs and grasses were less abundant). We also demonstrated per capita recruitment of Columbian sharpâtailed grouse is positively associated with lateâsummer greenness. Our results show that several suspected threats have measurable, populationâlevel impacts to Columbian sharpâtailed grouse within Idaho. Moreover, our results suggest ongoing changes occurring within the core range of Columbian sharpâtailed grouse, including loss of CRP cover to tilled agriculture and changes to wildfire and precipitation dynamics are likely to have negative effects on populations
Observations of C-Band Brightness Temperature and Ocean Surface Wind Speed and Rain Rate in Hurricanes Earl and Karl (2010)
No abstract availabl
Perlecan Domain V induces VEGf secretion in brain endothelial cells through integrin α5ÎČ1 and ERK-dependent signaling pathways.
Perlecan Domain V (DV) promotes brain angiogenesis by inducing VEGF release from brain endothelial cells (BECs) following stroke. In this study, we define the specific mechanism of DV interaction with the α(5)ÎČ(1) integrin, identify the downstream signal transduction pathway, and further investigate the functional significance of resultant VEGF release. Interestingly, we found that the LG3 portion of DV, which has been suggested to possess most of DV\u27s angio-modulatory activity outside of the brain, binds poorly to α(5)ÎČ(1) and induces less BEC proliferation compared to full length DV. Additionally, we implicate DV\u27s DGR sequence as an important element for the interaction of DV with α(5)ÎČ(1). Furthermore, we investigated the importance of AKT and ERK signaling in DV-induced VEGF expression and secretion. We show that DV increases the phosphorylation of ERK, which leads to subsequent activation and stabilization of eIF4E and HIF-1α. Inhibition of ERK activity by U0126 suppressed DV-induced expression and secretion of VEGR in BECs. While DV was capable of phosphorylating AKT we show that AKT phosphorylation does not play a role in DV\u27s induction of VEGF expression or secretion using two separate inhibitors, LY294002 and Akt IV. Lastly, we demonstrate that VEGF activity is critical for DV increases in BEC proliferation, as well as angiogenesis in a BEC-neuronal co-culture system. Collectively, our findings expand our understanding of DV\u27s mechanism of action on BECs, and further support its potential as a novel stroke therapy
Ploidy Variation in Multinucleate Cells Changes Under Stress
Ploidy variation is found in contexts as diverse as solid tumors, drug resistance in fungal infection, and normal development. Altering chromosome or genome copy number supports adaptation to fluctuating environments but is also associated with fitness defects attributed to protein imbalances. Both aneuploidy and polyploidy can arise from multinucleate states after failed cytokinesis or cell fusion. The consequences of ploidy variation in syncytia are difficult to predict because protein imbalances are theoretically buffered by a common cytoplasm. We examined ploidy in a naturally multinucleate fungus, Ashbya gossypii. Using integrated lac operator arrays, we found that chromosome number varies substantially among nuclei sharing a common cytoplasm. Populations of nuclei range from 1N to \u3e4N, with different polyploidies in the same cell and low levels of aneuploidy. The degree of ploidy variation increases as cells age. In response to cellular stress, polyploid nuclei diminish and haploid nuclei predominate. These data suggest that mixed ploidy is tolerated in these syncytia; however, there may be costs associated with variation as stress homogenizes the genome content of nuclei. Furthermore, the results suggest that sharing of gene products is limited, and thus there is incomplete buffering of ploidy variation despite a common cytosol
Beyond gene-disease validity: capturing structured data on inheritance, allelic requirement, disease-relevant variant classes, and disease mechanism for inherited cardiac conditions
Background:
As the availability of genomic testing grows, variant interpretation will increasingly be performed by genomic generalists, rather than domain-specific experts. Demand is rising for laboratories to accurately classify variants in inherited cardiac condition (ICC) genes, including secondary findings.
//
Methods:
We analyse evidence for inheritance patterns, allelic requirement, disease mechanism and disease-relevant variant classes for 65 ClinGen-curated ICC gene-disease pairs. We present this information for the first time in a structured dataset, CardiacG2P, and assess application in genomic variant filtering.
//
Results:
For 36/65 gene-disease pairs, loss of function is not an established disease mechanism, and protein truncating variants are not known to be pathogenic. Using the CardiacG2P dataset as an initial variant filter allows for efficient variant prioritisation whilst maintaining a high sensitivity for retaining pathogenic variants compared with two other variant filtering approaches.
//
Conclusions:
Access to evidence-based structured data representing disease mechanism and allelic requirement aids variant filtering and analysis and is a pre-requisite for scalable genomic testing
Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy
Background
A reliable system for grading operative difficulty of laparoscopic cholecystectomy would standardise description of findings and reporting of outcomes. The aim of this study was to validate a difficulty grading system (Nassar scale), testing its applicability and consistency in two large prospective datasets.
Methods
Patient and disease-related variables and 30-day outcomes were identified in two prospective cholecystectomy databases: the multi-centre prospective cohort of 8820 patients from the recent CholeS Study and the single-surgeon series containing 4089 patients. Operative data and patient outcomes were correlated with Nassar operative difficultly scale, using Kendallâs tau for dichotomous variables, or JonckheereâTerpstra tests for continuous variables. A ROC curve analysis was performed, to quantify the predictive accuracy of the scale for each outcome, with continuous outcomes dichotomised, prior to analysis.
Results
A higher operative difficulty grade was consistently associated with worse outcomes for the patients in both the reference and CholeS cohorts. The median length of stay increased from 0 to 4 days, and the 30-day complication rate from 7.6 to 24.4% as the difficulty grade increased from 1 to 4/5 (both pâ<â0.001). In the CholeS cohort, a higher difficulty grade was found to be most strongly associated with conversion to open and 30-day mortality (AUROCâ=â0.903, 0.822, respectively). On multivariable analysis, the Nassar operative difficultly scale was found to be a significant independent predictor of operative duration, conversion to open surgery, 30-day complications and 30-day reintervention (all pâ<â0.001).
Conclusion
We have shown that an operative difficulty scale can standardise the description of operative findings by multiple grades of surgeons to facilitate audit, training assessment and research. It provides a tool for reporting operative findings, disease severity and technical difficulty and can be utilised in future research to reliably compare outcomes according to case mix and intra-operative difficulty
Biobanking and consenting to research: a qualitative thematic analysis of young peopleâs perspectives in the North East of England
Background: Biobanking biospecimens and consent are common practice in paediatric research. We need to explore children and young peopleâs (CYP) knowledge and perspectives around the use of and consent to biobanking. This will ensure meaningful informed consent can be obtained and improve current consent procedures. Methods: We designed a survey, in co-production with CYP, collecting demographic data, views on biobanking, and consent using three scenarios: 1) prospective consent, 2) deferred consent, and 3) reconsent and assent at age of capacity. The survey was disseminated via the Young Personâs Advisory Group North England (YPAGne) and participating CYPâs secondary schools. Data were analysed using a qualitative thematic approach by three independent reviewers (including CYP) to identify common themes. Data triangulation occurred independently by a fourth reviewer. Results: One hundred two CYP completed the survey. Most were between 16â18Â years (63.7%, N = 65) and female (66.7%, N = 68). 72.3% had no prior knowledge of biobanking (N = 73). Acceptability of prospective consent for biobanking was high (91.2%, N = 93) with common themes: âaltruismâ, âpotential benefits outweigh individual riskâ, 'frugality', and â(in)convenienceâ. Deferred consent was also deemed acceptable in the large majority (84.3%, N = 86), with common themes: âaltruismâ, âbody integrityâ and âsample frugalityâ. 76.5% preferred to reconsent when cognitively mature enough to give assent (N = 78), even if parental consent was previously in place. 79.2% wanted to be informed if their biobanked biospecimen is reused (N = 80). Conclusion: Prospective and deferred consent acceptability for biobanking is high among CYP in the UK. Altruism, frugality, body integrity, and privacy are the most important themes. Clear communication and justification are paramount to obtain consent. Any CYP with capacity should be part of the consenting procedure, if possible
- âŠ