CLÍNICA AMPLIADA À ESCOLA INCLUSIVA DURANTE A PANDEMIA DA COVID-19: RELATO DE EXPERIÊNCIA DE ESTÁGIO PROFISSIONALIZANTE EM PSICOLOGIA DA PUC MINAS BETIM

Em março de 2020 a comunidade escolar do país entrou em quarentena para evitar a proliferação do coronavírus. O isolamento social prolongado exigiu que a formação em Psicologia incluísse novas formas de transmissão do saber e de prestação de serviços, usando das tecnologias digitais. O estágio profissionalizante da ênfase voltada ao estudo e as práticas da Psicologia nas “Políticas Sociais” do curso de psicologia da PUC-MG unidade de Betim, tem uma parceria como Centro de Referência e Apoio à Educação Inclusiva Rafael Veneroso, visando qualificar as(os) professores das escolas da rede municipal para tratar da inclusão usando de metodologias lúdicas e coletivas. O presente artigo visa apresentar o que pudemos fazer de forma remota para não deixarmos de prestar nosso serviço a rede municipal da cidade de Betim

Antioxidant activity and acute toxicity of Neoglaziovia variegata (Bromeliaceae)

Antioxidant activities of Neoglaziovia variegata were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching and was compared with ascorbic acid, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The total phenolics content of the extracts was determined by the Folin-Ciocalteu method. Total flavonoid was also determined. The most significant total phenolic content was of 543.50 ± 9.38 mg of gallic acid equivalent/g for ethyl acetate extract (AcOEt), which presented the best antioxidant activity (IC50 5.08 ± 0.20 μg/ml) for DPPH scavenging. The acute toxicity of Nv-EtOH was performed 2.0 g/kg intraperitoneally and 5.0 g/kg orally in mice. No mortality and no toxicity signs were observed, indicating low toxicity of the extract. Blood was removed after 14 days for laboratory analysis of hematological and biochemical parameters. Alterations of aspartate aminotransferase (AST) and creatinine were observed. The data obtained showed that the doses induced microscopic alterations in the liver and kidney. In conclusion, the Nv-EtOH can be considered of low toxicity.Keywords: Antioxidant activity, acute toxicity, Neoglaziovia variegata, Bromeliacea

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Intolerância na adolescência: a resistência de adolescentes LGBTI+ à homofobia

The article discusses the homophobia experienced by young people and adolescents, and the way they deal with this problem. Our objective is to identify the attitude of these subjects in situations of discrimination and intolerance due to sexual orientation. The theme was dealt with in three parts: in the first one, an analysis of the concept of adolescence is made; then, a historical-conceptual analysis of homosexualities within a psychoanalytical reference and, finally, an intertwining of concepts about intolerance due to homophobia which is, from an early age, faced, disguised in games by LGBTI + people.O presente artigo trata da questão da homofobia vivenciada por jovens e adolescentes e do modo como eles vêm lidando com essa problemática. O objetivo é identificar a postura desses jovens e adolescentes perante situações de discriminação e intolerância por orientação sexual. Percebeu-se a importância de tratar da temática das sexualidades na adolescência, mais especificamente as homossexualidades, visto que o homossexual continua sendo marginalizado na sociedade heteronormativa. Para tratar de um assunto tão complexo, o presente artigo se divide em três partes: na primeira faz-se uma análise do conceito de adolescência; na segunda, uma análise histórico-conceitual das homossexualidades e na terceira há um entrelaçamento de ambos os conceitos no que tange à intolerância propiciada pela homofobia, que é travestida em brincadeiras e enfrentada desde cedo por pessoas LGBTI+

Soroepidemiologia da doença de Newcastle em plantéis de avestruzes dos Estados da Bahia e de São Paulo Serologic occurrence of Newcastle disease in ostriches raised in Bahia and São Paulo

Estudos sorológicos em Avestruzes (Struthio camelus) são ferramentas úteis para analisar os riscos relacionados à Doença de Newcastle nesses plantéis e à avicultura nacional. No presente estudo, amostras de sangue foram obtidas de avestruzes de ambos os sexos, de diferentes faixas etárias e sem apresentação de sintomatologia clínica, criadas nos Estados da Bahia e de São Paulo com o objetivo de avaliar a presença de anticorpos contra o vírus da Doença de Newcastle por meio de ELISA indireto. Foram testadas 339 amostras provenientes do Estado da Bahia e 105 amostras do Estado de São Paulo. Apesar de os proprietários afirmarem que não foi utilizada vacina em seus animais, foi verificada positividade na Bahia de 17,9% e de 4,7% em São Paulo, em avestruzes, sugerindo contato com vírus vacinal ou de campo.Serological studies in ostriches (Struthio camelus) are important tools to assess the risk of Newcastle disease in these herds and to the national poultry industry. In the present study blood samples were obtained from male and female ostriches without symptoms of the disease, raised in Bahia and São Paulo in order to evaluate the presence of antibodies against Newcastle disease virus using an indirect ELISA. There were collected 339 samples in Bahia and 105 samples in São Paulo. Although the owners guarantee that animals were not vaccinated, it was verified the presence 17,9% positives in Bahia and 4,7% in São Paulo, suggesting contact with vaccinal or field strain

Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae <i>Tetradesmus obliquus</i>

The high protein content of several microalgal species makes them attractive and unconventional candidates for use in the food and pharmaceutical industries. Due to the robust cell walls of microalgae, cell rupture is necessary to improve the extraction of intracellular proteins. Thus, choosing a suitable cell-breaking treatment before protein extraction is a vital downstream processing step. Additionally, it is necessary to use an effective technique for monitoring and measuring the impact of rupture treatments on microalgal cell walls. In our study, Tetradesmus obliquus cells were disrupted using three different mechanical rupture methods: high-pressure homogenization (HPH), ultrasound (US), and ball milling (BM). The ruptured biomass cells were counted, and soluble proteins were extracted and quantified. The cell-counting technique did not detect any differences between intact and damaged cells after BM treatment because the dye (erythrosine B) did not permeate the microalgal biomass accurately. The US treatment promoted the highest yield of total protein extraction (19.95%), while the highest yields in the HPH and BM treatments were 15.68% and 14.11%, respectively. Since the cell breakage method affects protein extraction from microalgal biomass, protein release can be used as a central indicator of the degree of cell disruption