Torsion and bending of nucleic acids studied by subnanosecond time-resolved fluorescence depolarization of intercalated dyes

Subnanosecond time‐resolved fluorescence depolarization has been used to monitor the reorientation of ethidium bromide intercalated in native DNA, synthetic polynucleotide complexes, and in supercoiled plasmid DNA. The fluorescence polarization anisotropy was successfully analyzed with an elastic model of DNA dynamics, including both torsion and bending, which yielded an accurate value for the torsional rigidity of the different DNA samples. The dependence of the torsional rigidity on the base sequence, helical structure, and tertiary structure was experimentally observed. The magnitude of the polyelectrolyte contribution to the torsional rigidity of DNA was measured over a wide range of ionic strength, and compared with polyelectrolyte theories for the persistence length. We also observed a rapid initial reorientation of the intercalated ethidium which had a much smaller amplitude in RNA than in DNA

Large wind turbines: A utility option for the generation of electricity

The wind resource is such that wind energy generation has the potential to save 6-7 quads of energy nationally. Thus, the Federal Government is sponsoring and encouraging the development of cost effective and reliable wind turbines. One element of the Federal Wind Energy Programs, Large Horizontal Axis Wind Turbine Development, is managed by the NASA Lewis Research Center for the Department of Energy. There are several ongoing wind system development projects oriented primarily toward utility application within this program element. In addition, a comprehensive technology program supporting the wind turbine development projects is being conducted. An overview is presented of the NASA activities with emphasis on application of large wind turbines for generation of electricity by utility systems

Large wind turbines: A utility option for the generation of electricity

The economic and technical potential of wind energy in the United States is discussed. Particular attention is given to the status of wind turbine operational experience as well as the environmental posture of the technology

Time-resolved spectroscopy of macromolecules: Effect of helical structure on the torsional dynamics of DNA and RNA

The torsional rigidity of DNA and RNA is measured via the fluorescence depolarization technique

The contact of certain elastic shells with rigid flat surfaces Technical report no. 3

Stress and shear deformation analysis for simple symmetric elastic shells in contact with rigid flat surfac

Implant Combinations and Reimplanting Strategies for Yearling Steers Fed High Concentrate Diets

Crossbred yearling steers were used to determine the relative efficacy of specific anabolic implant combinations and sequences on feedlot performance and carcass traits. Steers were fed a high concentrate finishing diet for 112 days. lmplanting was done on days 1 and 42 of the feeding period. lmplanting improved (P \u3c .05) average daily gain (ADG) 22% and feed efficiency 15%. Implant treatment generally increased dry matter intake. Lmplanting increased (P\u3c.05) the rib eye area of carcasses 6.5% and tended to cause a reduction in percentage choice carcasses. The percentage of abscessed implants ranged from \u3c 1 % to 10%, depending on the type of implant used even though implant needles were disinfected between each use

Toward the End of Time

The null-brane space-time provides a simple model of a big crunch/big bang singularity. A non-perturbative definition of M-theory on this space-time was recently provided using matrix theory. We derive the fermion couplings for this matrix model and study the leading quantum effects. These effects include particle production and a time-dependent potential. Our results suggest that as the null-brane develops a big crunch singularity, the usual notion of space-time is replaced by an interacting gluon phase. This gluon phase appears to constitute the end of our conventional picture of space and time.Comment: 31 pages, reference adde

Comparative testing of anthropomorphic dummy and a standardized impactor for glazing materials

Consumer Product Safety Commission, Bethesda, Office of the Medical Director, Md.http://deepblue.lib.umich.edu/bitstream/2027.42/740/2/35897.0001.001.pd

Quantification of thoracic response and injury: the gathering of data. appendix B: rib test data. Final report

Notes: Report Covers the period July 1974 - Aug 1982National Highway Traffic Safety Administration, Washington, D.C.http://deepblue.lib.umich.edu/bitstream/2027.42/291/2/72989.0001.001.pd