1,021 research outputs found
Single-fluorophore orientation determination with multiview polarized illumination : modeling and microscope design
Author Posting. © Optical Society of America, 2017. This article is posted here by permission of Optical Society of America for personal use, not for redistribution. The definitive version was published in Optics Express 25 (2017): 31309-31325, doi:10.1364/OE.25.031309.We investigate the use of polarized illumination in multiview microscopes for determining the orientation of single-molecule fluorescence transition dipoles. First, we relate the orientation of single dipoles to measurable intensities in multiview microscopes and develop an information-theoretic metricâthe solid-angle uncertaintyâto compare the ability of multiview microscopes to estimate the orientation of single dipoles. Next, we compare a broad class of microscopes using this metricâsingle- and dual-view microscopes with varying illumination polarization, illumination numerical aperture (NA), detection NA, obliquity, asymmetry, and exposure. We find that multi-view microscopes can measure all dipole orientations, while the orientations measurable with single-view microscopes is halved because of symmetries in the detection process. We also find that choosing a small illumination NA and a large detection NA are good design choices, that multiview microscopes can benefit from oblique illumination and detection, and that asymmetric NA microscopes can benefit from exposure asymmetry.National Institute of Health (NIH) (R01GM114274, R01EB017293)
Gas and dust in the Beta Pictoris Moving Group as seen by the Herschel Space Observatory
Context. Debris discs are thought to be formed through the collisional
grinding of planetesimals, and can be considered as the outcome of planet
formation. Understanding the properties of gas and dust in debris discs can
help us to comprehend the architecture of extrasolar planetary systems.
Herschel Space Observatory far-infrared (IR) photometry and spectroscopy have
provided a valuable dataset for the study of debris discs gas and dust
composition. This paper is part of a series of papers devoted to the study of
Herschel PACS observations of young stellar associations.
Aims. This work aims at studying the properties of discs in the Beta Pictoris
Moving Group (BPMG) through far-IR PACS observations of dust and gas.
Methods. We obtained Herschel-PACS far-IR photometric observations at 70, 100
and 160 microns of 19 BPMG members, together with spectroscopic observations of
four of them. Spectroscopic observations were centred at 63.18 microns and 157
microns, aiming to detect [OI] and [CII] emission. We incorporated the new
far-IR observations in the SED of BPMG members and fitted modified blackbody
models to better characterise the dust content.
Results. We have detected far-IR excess emission toward nine BPMG members,
including the first detection of an IR excess toward HD 29391.The star HD
172555, shows [OI] emission, while HD 181296, shows [CII] emission, expanding
the short list of debris discs with a gas detection. No debris disc in BPMG is
detected in both [OI] and [CII]. The discs show dust temperatures in the range
55 to 264 K, with low dust masses (6.6*10^{-5} MEarth to 0.2 MEarth) and radii
from blackbody models in the range 3 to 82 AU. All the objects with a gas
detection are early spectral type stars with a hot dust component.Comment: 12 pages, 7 figures, 6 table
Beyond "the Relationship between the Individual and Society": broadening and deepening relational thinking in group analysis
The question of âthe relationship between the individual and societyâ has troubled group analysis since its inception. This paper offers a reading of Foulkes that highlights the emergent, yet evanescent, psychosocial ontology in his writings, and argues for the development of a truly psychosocial group analysis, which moves beyond the individual/society dualism. It argues for a shift towards a language of relationality, and proposes new theoretical resources for such a move from relational sociology, relational psychoanalysis and the âmatrixial thinkingâ of Bracha Ettinger which would broaden and deepen group analytic understandings of relationality
DZ Cha: a bona fide photoevaporating disc
DZ Cha is a weak-lined T Tauri star (WTTS) surrounded by a bright
protoplanetary disc with evidence of inner disc clearing. Its narrow \Ha line
and infrared spectral energy distribution suggest that DZ Cha may be a
photoevaporating disc. We aim to analyse the DZ Cha star + disc system to
identify the mechanism driving the evolution of this object. We have analysed
three epochs of high resolution optical spectroscopy, photometry from the UV up
to the sub-mm regime, infrared spectroscopy, and J-band imaging polarimetry
observations of DZ Cha. Combining our analysis with previous studies we find no
signatures of accretion in the \Ha line profile in nine epochs covering a
time baseline of years. The optical spectra are dominated by
chromospheric emission lines, but they also show emission from the forbidden
lines [SII] 4068 and [OI] 6300 that indicate a disc outflow. The
polarized images reveal a dust depleted cavity of au in radius and two
spiral-like features, and we derive a disc dust mass limit of
M_\mathrm{dust}
80 \MJup) companions are detected down to 0\farcs07 ( au,
projected). The negligible accretion rate, small cavity, and forbidden line
emission strongly suggests that DZ Cha is currently at the initial stages of
disc clearing by photoevaporation. At this point the inner disc has drained and
the inner wall of the truncated outer disc is directly exposed to the stellar
radiation. We argue that other mechanisms like planet formation or binarity
cannot explain the observed properties of DZ Cha. The scarcity of objects like
this one is in line with the dispersal timescale ( yr) predicted
by this theory. DZ Cha is therefore an ideal target to study the initial stages
of photoevaporation.Comment: A&A in press, language corrections include
Polar Smectic Films
We report on a new experimental procedure for forming and studying polar
smectic liquid crystal films. A free standing smectic film is put in contact
with a liquid drop, so that the film has one liquid crystal/liquid interface
and one liquid crystal/air interface. This polar environment results in changes
in the textures observed in the film, including a boojum texture and a
previously unobserved spiral texture in which the winding direction of the
spiral reverses at a finite radius from its center. Some aspects of these
textures are explained by the presence of a Ksb term in the bulk elastic free
energy density that favors a combination of splay and bend deformations.Comment: 4 pages, REVTeX, 3 figures, submitted to PR
Dissection of Molecular Assembly Dynamics by Tracking Orientation and Position of Single Molecules in Live Cells
Regulation of order, such as orientation and conformation, drives the function of most molecular assemblies in living cells but remains difficult to measure accurately through space and time. We built an instantaneous fluorescence polarization microscope, which simultaneously images position and orientation of fluorophores in living cells with single-molecule sensitivity and a time resolution of 100 ms. We developed image acquisition and analysis methods to track single particles that interact with higher-order assemblies of molecules. We tracked the fluctuations in position and orientation of molecules from the level of an ensemble of fluorophores down to single fluorophores. We tested our system in vitro using fluorescently labeled DNA and F-actin, in which the ensemble orientation of polarized fluorescence is known. We then tracked the orientation of sparsely labeled F-actin network at the leading edge of migrating human keratinocytes, revealing the anisotropic distribution of actin filaments relative to the local retrograde flow of the F-actin network. Additionally, we analyzed the position and orientation of septin-GFP molecules incorporated in septin bundles in growing hyphae of a filamentous fungus. Our data indicate that septin-GFP molecules undergo positional fluctuations within âŒ350 nm of the binding site and angular fluctuations within âŒ30° of the central orientation of the bundle. By reporting position and orientation of molecules while they form dynamic higher-order structures, our approach can provide insights into how micrometer-scale ordered assemblies emerge from nanoscale molecules in living cells
Anomalous diffusion in polymers: long-time behaviour
We study the Dirichlet boundary value problem for viscoelastic diffusion in
polymers. We show that its weak solutions generate a dissipative semiflow. We
construct the minimal trajectory attractor and the global attractor for this
problem.Comment: 13 page
Radio emission of extensive air shower at CODALEMA: Polarization of the radio emission along the v*B vector
Cosmic rays extensive air showers (EAS) are associated with transient radio
emission, which could provide an efficient new detection method of high energy
cosmic rays, combining a calorimetric measurement with a high duty cycle. The
CODALEMA experiment, installed at the Radio Observatory in Nancay, France, is
investigating this phenomenon in the 10^17 eV region. One challenging point is
the understanding of the radio emission mechanism. A first observation
indicating a linear relation between the electric field produced and the cross
product of the shower axis with the geomagnetic field direction has been
presented (B. Revenu, this conference). We will present here other strong
evidences for this linear relationship, and some hints on its physical origin.Comment: Contribution to the 31st International Cosmic Ray Conference, Lodz,
Poland, July 2009. 4 pages, 8 figures. v2: Typo fixed, arxiv references adde
α-chloralose poisoning in a cat
A five-year-old domestic cat with acute unexplainable neurological signs was presented for postmortem examination. Clinically, the cat showed an acute onset of ataxia, depressed mentation and continuous twitching/ seizure activity in the morning after having been outside overnight. Despite immediate treatment, the cat# progressed within 24 hours to a comatose state, opisthotonus and severe miosis unresponsive to light. Given a poor prognosis, euthanasia was elected. Gross findings were disappointing and consisted of a nonspecific lung oedema and congested lungs and spleen. Surprisingly, within the stomach and intestines, fragments of cockshafers were found. Histological examination confirmed the gross findings and additionally showed evidence of mild brain oedema, but failed to identify a cause for the severe clinical signs. In a final attempt to solve the case, a urine sample was tested for toxic substances and it was found to contain a significant amount of α-chloralose. This finding was unexpected
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