The 4.2 ka event in the vegetation record of the central Mediterranean

In this paper, the variation in forest cover in the central Mediterranean region, reflected by percentage changes in the arboreal pollen record, has been examined in relation to the 4.2 ka event. A total of 36 well-dated and detailed pollen records from latitudes between 45 and 36 degrees N were selected and their vegetation dynamics between 5 and 3 ka examined in relation to the physiographic and climatic features of the study area and to the influence of human activity on past vegetation, as suggested by anthropogenic pollen indicators. We have found that the sites located between 43 and 45 degrees N do not show any significant vegetation change in correspondence with the 4.2 ka event. Several sites located on the Italian Peninsula between 39 and 43 degrees N show a marked opening of the forest, suggesting a vegetation response to the climate instability of the 4.2 ka event. Between 36 and 39 degrees N, a forest decline is always visible around 4.2 ka, and in some cases it is dramatic. This indicates that this region was severely affected by a climate change towards arid conditions that lasted a few hundred years and was followed by a recovery of forest vegetation in the Middle Bronze Age. Human activity, especially intense in southern Italy, may have been favored by this natural opening of vegetation. In Sardinia and Corsica, no clear change in vegetation is observed at the same time. We suggest that during the 4.2 ka event southern Italy and Tunisia were under the prevalent influence of a north African climate system characterized by a persistent high-pressure cell

Decline of evergreen vegetation in the central Mediterranean regions during the 4 ka BP drought event

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Holocene environmental instability in the wetland north of the Tiber delta (Rome, Italy): sea-lake-man interactions

Combined analyses of pollen, seeds, woods, micro-charcoal and non-pollen palynomorphs from Stagno di Maccarese, an artificially dried out coastal basin north of the Tiber delta now occupied by the Fiumicino Airport (Rome, Italy), document marked vegetation and environmental changes during the last 8300 years. Between 8300 and 5400 cal. a BP dense mixed deciduous and evergreen forests surrounded a eutrophic freshwater basin. An abrupt change around 5400 cal. a BP marks the transition to a marshy environment, due to a lowering of the water table. An increase of cereals and micro-charcoals matches the presence of a nearby Eneolithic settlement. Between 5100 and 2900 cal. a BP there is a remarkable expansion of riparian trees, indicating an increase of the water level. Between 2900 and 2000 cal. a BP, a new development of marshlands points to a progressive lowering of the lake. After 2000 cal. a BP, during the Roman exploitation of the area, an expansion of arboreal vegetation is recorded, characterized by evergreen and deciduous oak-dominated forests, while an extensive chenopods marshland matches the presence of saltworks. On the whole, the Stagno di Maccarese area appears very unstable, due to changes in lake level, introgression of marine water, eutrophic phases, flood events, desiccations and openings of the forest vegetation

Mid to late Holocene environmental changes along the coast of western Sardinia (Mediterranean Sea)

Multiproxy analysis composed of biostratigraphy and pollen analysis allowed reconstructing the palaeoecological and palaeoenvironmental evolution of the Tirso river coastal plain in Sardinia (NW Mediterranean) in the last 6 millennia. We demonstrated that interplay between littoral and fluvial processes have significantly controlled the environmental evolution of the area and have played a key role in the pattern of historical and prehistorical settlements of this wide portion of western Sardinian coastline. At the end of Neolithic period (ca. 6.0 to 5.5 cal. ka BP) the area close to the shoreline was most likely characterized by large coastal lagoons intermittently connected to the open sea. Such saltwater influence is corroborated by faunal and pollen assemblages found in the landward portion of the Tirso coastal plain up to 2 km inland from the modern shoreline. Our data robustly document the end of the transgressive trend at ca. 5.5 cal. ka BP, and a dominant fluvial sedimentation since Final Neolithic period. At this time, a progradational trend started, causing the seaward migration of shoreline and, consequently, of the barrier-lagoon system. The major landscape modification tracked along the last 6 millennia may also explain the low density of historical and prehistorical remains in the Tirso coastal plain, especially if compared to the nearby rocky area of Sinis Peninsula densely inhabited since the Neolithic. Our data further provide new insights into the Relative Sea Level (RSL) evolution in this sector of the Mediterranean. In particular, we improved the mid-Holocene RSL record in Sardinia, where only scarce data were previously available

Quaternary disappearance of tree taxa from Southern Europe: timing and trends

A hundred pollen and plant macrofossil records from the Iberian Peninsula, Southern France, the Italian Peninsula, Greece and the Aegean, and the southwestern Black Sea area formed the basis for a review of the Quaternary distribution and extirpation of tree populations from Southern Europe. Following a discussion of the caveats/challenges about using pollen data, the Quaternary history of tree taxa has been reconstructed with attention to Taxodium/Glyptostrobus, Sciadopitys, Cathaya, Cedrus, Tsuga, Eucommia, Engelhardia, Carya, Pterocarya, Parrotia, Liquidambar, and Zelkova. The timing of extinction, distributed over the whole Quaternary, appears very diverse from one region to the other, in agreement with current biodiversity in Southern Europe. The geographical patterns of persistence/disappearance of taxa show unexpected trends and rule out a simple North to South and/or West to East trend in extirpations. In particular, it is possible to detect disjunct populations (Engelhardia), long-term persistence of taxa in restricted regions (Sciadopitys), distinct populations/species/genera in different geographical areas (Taxodium type). Some taxa that are still widespread in Europe have undergone extirpation in Mediterranean areas in the lateglacial period and Holocene (Buxus, Carpinus betulus, Picea); they provide an indication of the modes of disappearance of tree populations that may be useful to evaluate correctly the vulnerability of modern fragmented plant populations. The demographic histories of tree taxa obtained by combined palaeobotanical and genetic studies is a most challenging field of research needed not only to assess species/population differentiation, but also to reach a better understanding of extinction processes, an essential task in the current global change scenario

La Piccola Era Glaciale e il suo clima tra mare, terra e arte

La Piccola Era Glaciale e il suo clima tra mare, terra e arte

Blackwater Fever Treated with Steroids in Nonimmune Patient, Italy

: Causes of blackwater fever, a complication of malaria treatment, are not completely clear, and immune mechanisms might be involved. Clinical management is not standardized. We describe an episode of blackwater fever in a nonimmune 12-year-old girl in Italy who was treated with steroids, resulting in a rapid clinical resolution

46, XX DSD due to androgen excess in monogenic disorders of steroidogenesis: Genetic, biochemical, and clinical features

The term ‘differences of sex development’ (DSD) refers to a group of congenital conditions that are associated with atypical development of chromosomal, gonadal, or anatomical sex. Disorders of steroidogenesis comprise autosomal recessive conditions that affect adrenal and gonadal enzymes and are responsible for some conditions of 46, XX DSD where hyperandrogenism interferes with chromosomal and gonadal sex development. Congenital adrenal hyperplasias (CAHs) are disorders of steroidogenesis that mainly involve the adrenals (21-hydroxylase and 11-hydroxylase deficiencies) and sometimes the gonads (3-beta-hydroxysteroidodehydrogenase and P450-oxidoreductase); in contrast, aromatase deficiency mainly involves the steroidogenetic activity of the gonads. This review describes the main genetic, biochemical, and clinical features that apply to the abovementioned conditions. The activities of the steroidogenetic enzymes are modulated by post-translational modifications and cofactors, particularly electron-donating redox partners. The incidences of the rare forms of CAH vary with ethnicity and geography. The elucidation of the precise roles of these enzymes and cofactors has been significantly facilitated by the identification of the genetic bases of rare disorders of steroidogenesis. Understanding steroidogenesis is important to our comprehension of differences in sexual development and other processes that are related to human reproduction and fertility, particularly those that involve androgen excess as consequence of their impairment

Untargeted Metabolic Profiling of 4-Fluoro-Furanylfentanyl and Isobutyrylfentanyl in Mouse Hepatocytes and Urine by Means of LC-HRMS

The diffusion of new psychoactive substances (NPS) is highly dynamic and the available substances change over time, resulting in forensic laboratories becoming highly engaged in NPS control. In order to manage NPS diffusion, efficient and innovative legal responses have been provided by several nations. Metabolic profiling is also part of the analytical fight against NPS, since it allows to identify the biomarkers of drug intake which are needed for the development of suitable analytical methods in biological samples. We have recently reported the characterization of two new analogs of fentanyl, i.e., 4-fluoro-furanylfentanyl (4F-FUF) and isobutyrylfentanyl (iBF), which were found for the first time in Italy in 2019; 4F-FUF was identified for the first time in Europe and was notified to the European Early Warning System. The goal of this study was the characterization of the main metabolites of both drugs by in vitro and in vivo experiments. To this end, incubation with mouse hepatocytes and intraperitoneal administration to mice were carried out. Samples were analyzed by means of liquid chromatography-high resolution mass spectrometry (LC–HRMS), followed by untargeted data evaluation using Compound Discoverer software with a specific workflow, designed for the identification of the whole metabolic pattern, including unexpected metabolites. Twenty metabolites were putatively annotated for 4F-FUF, with the dihydrodiol derivative appearing as the most abundant, whereas 22 metabolites were found for iBF, which was mainly excreted as nor-isobutyrylfentanyl. N-dealkylation of 4F-FUF dihydrodiol and oxidation to carbonyl metabolites for iBF were also major biotransformations. Despite some differences, in general there was a good agreement between in vitro and in vivo samples