Wastewater valorization: Practice around the world at pilot-and full-scale

LA/P/0140/2020Over the last few years, wastewater treatment plants (WWTPs) have been rebranded as water resource recovery facilities (WRRFs), which recognize the resource recovery potential that exists in wastewater streams. WRRFs contribute to a circular economy by not only producing clean water but by recovering valuable resources such as nutrients, energy, and other bio-based materials. To this aim, huge efforts in technological progress have been made to valorize sewage and sewage sludge, transforming them into valuable resources. This review summarizes some of the widely used and effective strategies applied at pilot-and full-scale settings in order to valorize the wastewater treatment process. An overview of the different technologies applied in the water and sludge line is presented, covering a broad range of resources, i.e., water, biomass, energy, nutrients, volatile fatty acids (VFA), polyhydroxyalkanoates (PHA), and exopolymeric substances (EPS). Moreover, guidelines and regulations around the world related to water reuse and resource valorization are reviewed.publishersversionpublishe

Microalgae-bacterial biomass outperforms PN-anammox biomass for oxygen saving in continuous-flow granular reactors facing extremely low-strength freshwater aquaculture streams

The dissolved oxygen (DO) concentration in water streams is one of the most important and critical quality parameters in aquaculture farms. The main objective of this study was to evaluate the potential of two Continuous Flow Granular Reactors, one based on Partial Nitrification-Anammox biomass (Aquammox CFGR) and the other on Microalgae-Bacteria biomass (AquaMab CFGR), for improving dissolved oxygen availability in the recirculation aquaculture systems (RAS). Both reactors treated the extremely low-strength effluents from a freshwater trout farm (1.39 mg NH4 +-N/L and 7.7 mg TOC/L). The Aquammox CFGR, removed up to 68% and 100% of ammonium and nitrite, respectively, but the DO concentration in the effluent was below 1 mg O2/L while the anammox activity was not maintained. In the AquaMab CFGR, bioaugmentation of aerobic granules with microalgae was attained, producing an effluent with DO concentrations up to 9 mg O2/L and removed up to 77% and 80% of ammonium and nitrite, respectively, which is expected to reduce the aeration costs in fish farms.info:eu-repo/semantics/publishedVersio

Granular sludge technology for valorization of water use: from high to low strength effluents in aquaculture

info:eu-repo/semantics/publishedVersio

Rapid start-up and stable maintenance of the mainstream nitritation process based on the accumulation of free nitrous acid in a pilot-scale two-stage nitritation-anammox system

Two-stage partial nitritation (PN) and anammox (AMX) systems showed promising results for applying autotrophic nitrogen removal under mainstream conditions. In this study, a pilot-scale (600 L per reactor) two-stage PN/AMX system was installed in a municipal wastewater treatment plant (WWTP) provided with a high-rate activated sludge (HRAS) system for organic carbon removal. The PN/AMX system was operated without temperature control (ranging from 11 to 28 °C) and was subjected to the same variations in wastewater characteristics as the WWTP (22 to 63 mg NH4+- N/L). The developed strategy is simple, does not require the addition of chemicals and is characterised by short start-up periods. The PN process was established by applying a high hydraulic load and maintained by in situ accumulated free nitrous acid (FNA) of 0.015–0.2 mg HNO2-N/L. Based on pH value, a controlled aeration strategy was applied to achieve the target nitrite to ammonium ratio in the effluent (1.1 g NO2–-N/g NH4+-N) to feed the AMX reactor. Although NOB were not fully washed out from the system, nitrite accumulation remained (>99 %) stable with no evidence of NOB activity. In the AMX reactor, an overall nitrogen removal efficiency of 80 % was achieved. Regarding effluent quality, 12 ± 3 mg TN/L was obtained, but 5 mg NO3–-N/L was already in the HRAS effluent. The relative abundance of NOB showed a strong negative correlation with the FNA concentration, providing a good strategy for establishing PN under mainstream conditionsThis work has been financed by the European Commission (EU) through the LIFE project ZERO WASTE WATER (LIFE19 ENV/ES/000631), the Waterworks 2014 Cofounded Call (Water JPI/Horizon) through the Pioneer_STP (PCIN-2015-022 MINECO(AEI)/ID 199 (UE)) and by the Spanish Government (AEI) through GRANDSEA (CTM2014-55397-JIN), TREASURE (CTQ2017-83225-C2-1-R) and ECOPOLYVER (PID2020-112550RB-C21 and PID2020-112550RB-C22) projects. Alba Pedrouso also acknowledges the Xunta de Galicia (Spain) for her postdoctoral fellowship (ED481B-2021-041). Authors from the USC belong to the Galician Competitive Research Group (GRC D431C-2021/37)S

Recovery of Polyhydroxyalkanoates from Cooked Mussel Processing Wastewater at High Salinity and Acidic Conditions

Polyhydroxyalkanoates (PHA) are biodegradable polymers that can be intracellularly produced by microorganisms valorizing organic-rich wastes. In the present study, a PHA production system was fed with mussel cooker wastewater after acidogenic fermentation. Besides low pH (4.0 ± 0.3) and high salt (21.7 ± 2.9 g NaCl/L) concentrations, this wastewater also contained nitrogen concentrations (0.8 ± 0.1 g N/L), which were previously reported to be a challenge to the PHA accumulating bacteria enrichment. Bacteria with a PHA storage capacity were selected in an enrichment sequencing batch reactor (SBR) after 60 days of operation. The enriched mixed microbial culture (MMC) was mainly formed by microorganisms from phylum Bacteroidetes, and genera Azoarcus, Comamonas and Thauera from phylum Proteobacteria. The MMC was able to accumulate up to 25 wt% of PHA that was mainly limited by the wastewater nitrogen content, which promoted biomass growth instead of PHA accumulation. Indeed, when the presence of nutrient was limited, PHA stored in the accumulation reactor increased to up to 40.9 wt%. This work demonstrated the feasibility of the enrichment of a MMC with a PHA storage ability valorizing the fish-canning industrial wastewater at low pH, which is generally difficult to treat in wastewater treatment plants

Monitoring morphological changes of suspended sludge during aerobic granulation process

Aerobic granular sludge (AGS) technology depends on the growth of a self immobilizing microbial community to form granules, typically achieved through environmental and operational selective pressures. Long star-up periods and granules stability in long-term operation are described as the main drawbacks of this technology. During granulation, it is expected that most of the sludge achieve a granular form however often this is not the case. Microbial flocs and filamentous bacteria may endure in the system, potentially affecting granulation and/or granules stability.Quantitative image analysis (QIA) has helped researchers to understand microbial population dynamics in activated sludge, e.g. identifying bad settling properties phenomena. In this work, we used QIA to monitor morphological changes of suspended sludge during an aerobic granulation process with two sodium acetate concentrations; i.e. R1: 250 mgCOD.L-1; R2: 500 mgCOD.L-1. R1 and R2 reached good settling properties, achieving sludge volume index (SVI5) of 36 and 55 mL.g-1, respectively. However, granules were only visually observable in R1. QIA showed differences in aggregates with equivalent diameter of 100 μm ≤ Deq ≤ 650 μm, between both reactors in early stages of granulation. After 18 days, these aggregates were similar in key parameters such diameter, length and width, day from which a higher increase was observed in R1 than in R2. After 22 days, these aggregates represented 94% and 62% of the total projected area in R1 and R2, respectively. Interestingly, differences in aggregates appeared earlier in the reactors operation by morphological descriptors. In fact from day 11 onwards, aggregates in R1 exhibited higher compactness and robustness than in R2. Furthermore, the prevalence of filamentous bacteria in R2 might have been the reason why microbial aggregates could not achieve granular form. In R1, the total length of filaments (TL) remained below 3.3 mm.μL-1 from day 25, whereas in R2, TL continued to increase up to 16.3 mm.μL-1. Overall, results showed that QIA could be used to monitor morphological changes of activated sludge during an aerobic granulation process. This could be particularly useful to decrease the long-periods required for granulation, and also to monitor AGS system stability in long term operations.info:eu-repo/semantics/publishedVersio

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

The biosynthesis of polyhydroxyalkanoates (PHAs) from industrial wastes by mixed microbial cultures (MMCs) enriched in PHA-accumulating bacteria is a promising technology to replace petroleum-based plastics. However, the populations’ dynamics in the PHA-accumulating MMCs are not well known. Therefore, the main objective of this study was to address the shifts in the size and structure of the bacterial communities in two lab-scale sequencing batch reactors (SBRs) fed with fish-canning effluents and operated under non-saline (SBR-N, 0.5 g NaCl/L) or saline (SBR-S, 10 g NaCl/L) conditions, by using a combination of quantitative PCR and Illumina sequencing of bacterial 16S rRNA genes. A double growth limitation (DGL) strategy, in which nitrogen availability was limited and uncoupled to carbon addition, strongly modulated the relative abundances of the PHA-accumulating bacteria, leading to an increase in the accumulation of PHAs, independently of the saline conditions (average 9.04 wt% and 11.69 wt%, maximum yields 22.03 wt% and 26.33% SBR-N and SBR-S, respectively). On the other hand, no correlations were found among the PHAs accumulation yields and the absolute abundances of total Bacteria, which decreased through time in the SBR-N and did not present statistical differences in the SBR-S. Acinetobacter, Calothrix, Dyella, Flavobacterium, Novosphingobium, Qipengyuania, and Tsukamurella were key PHA-accumulating genera in both SBRs under the DGL strategy, which was revealed as a successful tool to obtain a PHA-enriched MMC using fish-canning effluents

Sequencing versus continuous granular sludge reactor for the treatment of freshwater aquaculture effluents

Ammonium and nitrite levels in water are crucial for fish health preservation and growth maintenance in freshwater aquaculture farms, limiting water recirculation. The aim of the present work was the evaluation and comparison of two granular sludge reactors which were operated to treat freshwater aquaculture streams at laboratory-scale: an Aerobic Granular Sludge - Sequencing Batch Reactor (AGS-SBR) and a Continuous Flow Granular Reactor (CFGR). Both units were fed with a synthetic medium mimicking an aquaculture recycling water (1.9–2.9 mg N/L), with low carbon content, and operational temperature varied between 17 and 25 °C. The AGS-SBR, inoculated with mature granules from a full-scale wastewater treatment plant, achieved high carbon and ammonium removal during the 157 operational days. Even at low hydraulic retention time (HRT), varying from 474 to 237 min, ammonium removal efficiencies of approximately 87–100% were observed, with an ammonium removal rate of approximately 14.5 mg NH4+-N/(L⋅d). Partial biomass washout occurred due to the extremely low carbon and nitrogen concentrations in the feeding, which could only support the growth of a small portion of bacteria, but no major changes on the reactor removal performance were observed. The CFGR was inoculated with activated sludge and operated for 98 days. Biomass granulation occurred in 7 days, improving the settling properties due to a high up-flow velocity of 11 m/h and an applied HRT of 5 min. The reactor presented mature granules after 32 days, achieving an average diameter of 1.9 mm at day 63. The CFGR ammonium removal efficiencies were of approximately 10–20%, with ammonium removal rates of 90.0 mg NH4+-N/(L⋅d). The main biological processes taking place in the AGS-SBR were nitrification and heterotrophic growth, while in the CFGR the ammonium removal occurred only by heterotrophic assimilation, with the reactor also presenting complete and partial denitrification, which caused nitrite production. Comparing both systems, the CFGR achieved 6 times higher ammonium removal rates than the AGS-SBR, being suitable for treating extremely high flows. On the other hand, the AGS-SBR removed almost 100% of ammonium content in the wastewater, discharging a better quality effluent, less toxic for the fish but treated lower flows.info:eu-repo/semantics/acceptedVersio