Satzverständnisleistungen von Kindern mit und ohne SSES im TROG-D und einer schweizerdeutschen Adaptation

Background: Certain challenges are associated with identifying children with speech comprehension disorders, as those children typically acquire compensatory strategies of communication, often resul- ting in late identification. In addition, speech therapists in the German-speaking part of Switzerland must employ assessment instruments that are designed for standard German and not for Swiss Ger- man. Those instruments are therefore executed in standard German or must be spontaneously transla- ted into Swiss German. Until now it has been unclear which of these options do the Swiss German-spea- king children justice. Aims: The aim of this study was to compare the sentence comprehension performances of monolingu- al children with and without specific language impairment (SLI) on a test designed in standard German and on a Swiss German translation of the same test. This study also compared the sentence comprehen- sion performances in both versions of the test within the groups. Methods: The sample consists of 481 children living in the canton of Bern (CH). At the time of testing, they were currently attending the grades of kindergarten up to the third grade. The children were tested with the TROG-D (Fox 2013) and with a Swiss German translation of this test. The sequence of the lan- guage versions was randomized. In the statistical analysis, children with SLI (diagnosed through speech language therapist) were matched with children without SLI. Statistical significance was tested by the cal- culation of paired t-tests. Results: The only statistically significant difference in assessment scores occurred in children with SLI compared with children without SLI, when assessed with the Swiss German translation of the test (p<.05, d=.41). The performance in standard German did not differ between groups. Within the groups, no dif- ferences between the sentence comprehension performances in standard German and Swiss German were found. Conclusions: The need for a Swiss German adaptation of the test seems questionable. However, differen- tiation of the sentence comprehension performances between children with and without SLI was only possible in the Swiss German version of the test. This result may be due to the low performance of the children without SLI in the standard German test, but there is insufficient data to confirm this possibi- lity. Further data analyses and controlled studies are needed to clarify the diagnostic issues addressed in the presented study more thoroughly

FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors

Replication-dependent histone genes are up-regulated during the G1/S phase transition to meet the requirement for histones to package the newly synthesized DNA. In mammalian cells, this increment is achieved by enhanced transcription and 3′ end processing. The non-polyadenylated histone mRNA 3′ ends are generated by a unique mechanism involving the U7 small ribonucleoprotein (U7 snRNP). By using affinity purification methods to enrich U7 snRNA, we identified FUS/TLS as a novel U7 snRNP interacting protein. Both U7 snRNA and histone transcripts can be precipitated by FUS antibodies predominantly in the S phase of the cell cycle. Moreover, FUS depletion leads to decreased levels of correctly processed histone mRNAs and increased levels of extended transcripts. Interestingly, FUS antibodies also co-immunoprecipitate histone transcriptional activator NPAT and transcriptional repressor hnRNP UL1 in different phases of the cell cycle. We further show that FUS binds to histone genes in S phase, promotes the recruitment of RNA polymerase II and is important for the activity of histone gene promoters. Thus, FUS may serve as a linking factor that positively regulates histone gene transcription and 3′ end processing by interacting with the U7 snRNP and other factors involved in replication-dependent histone gene expressio

FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors.

Replication-dependent histone genes are up-regulated during the G1/S phase transition to meet the requirement for histones to package the newly synthesized DNA. In mammalian cells, this increment is achieved by enhanced transcription and 3' end processing. The non-polyadenylated histone mRNA 3' ends are generated by a unique mechanism involving the U7 small ribonucleoprotein (U7 snRNP). By using affinity purification methods to enrich U7 snRNA, we identified FUS/TLS as a novel U7 snRNP interacting protein. Both U7 snRNA and histone transcripts can be precipitated by FUS antibodies predominantly in the S phase of the cell cycle. Moreover, FUS depletion leads to decreased levels of correctly processed histone mRNAs and increased levels of extended transcripts. Interestingly, FUS antibodies also co-immunoprecipitate histone transcriptional activator NPAT and transcriptional repressor hnRNP UL1 in different phases of the cell cycle. We further show that FUS binds to histone genes in S phase, promotes the recruitment of RNA polymerase II and is important for the activity of histone gene promoters. Thus, FUS may serve as a linking factor that positively regulates histone gene transcription and 3' end processing by interacting with the U7 snRNP and other factors involved in replication-dependent histone gene expression

Lesen in der Fremdsprache Französisch

illustratorDiese Publikation widmet sich dem Erwerb von Lesekompetenzen in der Fremdsprache Französisch bei Schülerinnen und Schülern mit und ohne (Schrift-)Sprachstörungen in der dritten Primarstufe. Die Längsschnittstudie liefert empirische Evidenz für Unterschiede in verschiedenen Teillesekompetenzen zu Beginn des frühen Fremdsprachenunterrichts. Das für die Datenerhebung entwickelte Screening, dessen empirische Validierung und die Berechnung von Strukturgleichungsmodellen zur Überprüfung von Einflussfaktoren auf die Lesekompetenzen in Französisch werden präsentiert. Die Forschungsresultate zeigen, dass der Leseerwerb in der Fremdsprache Französisch von den hierarchieniederen Lesekompetenzen in der Erstschriftsprache Deutsch und den Erwerbsmechanismen auf kognitiv-linguistischer Prozessebene beeinflusst ist. Die Unterschiede in den Teillesekompetenzen sprechen für eine frühzeitige Überprüfung des Lernstandes und gezielte Fördermassnahmen, die in der Publikation skizziert werden

Lesen in der Fremdsprache Französisch: Kompetenzen von Drittklässlerinnen und Drittklässlern mit unterschiedlichen Schrift- und Sprachfähigkeiten in der Erstsprache Deutsch

Diese Publikation widmet sich dem Erwerb von Lesekompetenzen in der Fremdsprache Französisch bei Schülerinnen und Schülern mit und ohne (Schrift-)Sprachstörungen in der dritten Primarstufe. Die Längsschnittstudie liefert empirische Evidenz für Unterschiede in verschiedenen Teillesekompetenzen zu Beginn des frühen Fremdsprachenunterrichts. Das für die Datenerhebung entwickelte Screening, dessen empirische Validierung und die Berechnung von Strukturgleichungsmodellen zur Überprüfung von Einflussfaktoren auf die Lesekompetenzen in Französisch werden präsentiert. Die Forschungsresultate zeigen, dass der Leseerwerb in der Fremdsprache Französisch von den hierarchieniederen Lesekompetenzen in der Erstschriftsprache Deutsch und den Erwerbsmechanismen auf kognitiv-linguistischer Prozessebene beeinflusst ist. Die Unterschiede in den Teillesekompetenzen sprechen für eine frühzeitige Überprüfung des Lernstandes und gezielte Fördermassnahmen, die in der Publikation skizziert werden

Lesen in der Fremdsprache Französisch. Kompetenzen von Drittklässlerinnen und Drittklässlern mit unterschiedlichen Schrift- und Sprachfähigkeiten in der Erstsprache Deutsch

Diese Publikation widmet sich dem Erwerb von Lesekompetenzen in der Fremdsprache Französisch bei Schülerinnen und Schülern mit und ohne (Schrift-)Sprachstörungen in der dritten Primarstufe. Die Längsschnittstudie liefert empirische Evidenz für Unterschiede in verschiedenen Teillesekompetenzen zu Beginn des frühen Fremdsprachenunterrichts. Das für die Datenerhebung entwickelte Screening, dessen empirische Validierung und die Berechnung von Strukturgleichungsmodellen zur Überprüfung von Einflussfaktoren auf die Lesekompetenzen in Französisch werden präsentiert. Die Forschungsresultate zeigen, dass der Leseerwerb in der Fremdsprache Französisch von den hierarchieniederen Lesekompetenzen in der Erstschriftsprache Deutsch und den Erwerbsmechanismen auf kognitiv-linguistischer Prozessebene beeinflusst ist. Die Unterschiede in den Teillesekompetenzen sprechen für eine frühzeitige Überprüfung des Lernstandes und gezielte Fördermassnahmen, die in der Publikation skizziert werden. (DIPF/Orig.

Lettres de Alexandre de Humboldt à Varnhagen von Ense (1827-1858) / édition française autorisée... [traduit par C.-F. Girard]

Comprend : [Extraits du Journal de Varnhagen et lettres à Humboldt]Contient une table des matièresAvec mode text

Additional file 2: Figure S2. of Serum metabolic profiling identified a distinct metabolic signature in patients with idiopathic pulmonary fibrosis – a potential biomarker role for LysoPC

MS (top, HDMSE experiment) and MS/MS spectra (bottom, HDMSE experiment) of the metabolic features eluting at 5.9 min acquired in the positive ESI mode and potentially assigned to the [M + H]+ 3-hydroxydecanoyl carnitine ion. The detection of the characteristic acylcarnitine fragment [C4H5O2]+ at m/z 85.0284 was confirmed by MS/MS spectra (bottom, HDMSE experiment). (PDF 99 kb

Serum Free Light Chain Assay: Shift Toward a Higher κ/λ Ratio.

BACKGROUND The analysis of serum free light chains (FLCs) is clinically relevant for the diagnosis and therapeutic management of clonal plasma cell disorders. This study compares the performance of monoclonal and polyclonal FLC κ and λ assays in clinical samples determined in a single academic center. METHODS Serum FLCs were analyzed from 102 patients using the Freelite (Binding Site) and N Latex (Siemens) assays on the BN ProSpec System (Siemens). When available, data for protein electrophoresis, immunofixation, C-reactive protein, and estimated glomerular filtration rate (eGFR) were combined with FLC results to evaluate performance. RESULTS Method evaluation showed acceptable imprecision and inaccuracy measures of <4.4% and 12.9%, respectively. Poor agreement between the methods was observed, including constant and proportional bias and poor correlation (Kendall τ, 0.671-0.901). The N Latex assay was not affected by the renal impairment estimated by eGFR, unlike the FLC κ/λ ratio results by the Freelite assay. With the Freelite assay, 98% of putative controls without monoclonal gammopathy (n = 42) showed a κ/λ ratio that was above the median of the standard diagnostic range or renal diagnostic range. A shift toward higher κ/λ ratios was also observed when retrospective data between 2011 and 2017 were compared. CONCLUSIONS Unlike the Freelite assay, κ/λ ratios analyzed with the N Latex assay were not affected by renal failure. Both methods showed acceptable performances using nephelometry, but they were poorly correlated. A shift toward κ/λ ratios might impair the specificity of borderline increased κ/λ results. This should be considered when interpreting FLC κ and λ results

Exhaled breath condensate as a potential biomarker tool for idiopathic pulmonary fibrosis - a pilot study.

Idiopathic pulmonary fibrosis (IPF) is a devastating lung disease with poor survival. There is an urgent need to better diagnose and monitor IPF patients as new treatments are now available which slow down disease progression. Exhaled breath condensate (EBC) is easily and non-invasively collected, but analysis of potential biomarkers is difficult due to low concentrations and methodological limitations. We now used a non-targeted metabolomics approach to identify further discriminatory metabolic profiles that distinguish IPF patients from controls.&#13; We collected EBC from 10 stable IPF patients and 10 lung healthy controls. Samples were analyzed by ultra high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) in positive and negative ion mode. An in-house established bioinformatics pipeline was applied for data processing and analysis. In total 31 metabolites were found to be discriminative between IPF patients and controls. By univariate statistics of both datasets, 8 metabolic features remained significant (standardized abundance, FDR p-value &lt; 0.05). Of them, all were detected in positive ion mode. Changes up to 3-fold were observed and all candidates were down-regulated in IPF patients compared to controls. Even though a discriminative metabolite signature was found between IPF patients and controls, the identities of the individual metabolites still remain elusive.&#13; Our preliminary results identified a distinguished EBC profile of IPF patients compared to controls. Although these results need to be confirmed in additional individuals, EBC sampling as a method to diagnose and/or monitor IPF patients is a promising, exciting new method, which deserves further exploration.&#13; The EBC samples have been obtained within the clinical trial NCT02173145 at baseline